• Ribonucleotide reductases are essential enzymes which synthesize deoxyribonucleotides used in the replication of DNA. (ibecbarcelona.eu)
  • Every organism uses ribonucleotide reductase (RNR) enzymes to make the nucleotide building blocks needed for DNA replication and repair," says lead author Amie Boal, an assistant professor of chemistry and of biochemistry and molecular biology at Penn State. (futurity.org)
  • An enzyme of the oxidoreductase class that catalyzes the formation of 2'-deoxyribonucleotides from the corresponding ribonucleotides using NADPH as the ultimate electron donor. (nih.gov)
  • The diiron(II) cluster in the R2 subunit of Escherichia coli ribonucleotide reductase (RNR) activates oxygen to generate a μ-oxodiiron(III) cluster and the stable tyrosyl radical that is critical for the conversion of ribonucleotides to deoxyribonucleotides. (psu.edu)
  • Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. (proteopedia.org)
  • RNRs perform complex chemistry to convert ribonucleotides-the building blocks of RNA, which are present in the cell-into deoxyribonucleotides-the building blocks of DNA. (futurity.org)
  • The R2 subunit of Escherichia coli ribonucleotide reductase contains a dinuclear iron center that generates a catalytically essential stable tyrosyl radical by one electron oxidation of a nearby tyrosine residue. (proteopedia.org)
  • His current research interests focus on the identification of new antimicrobial therapies and unravel the molecular mechanism underlying the transcripcional regulation of bacterial ribonucleotide reductase genes. (ibecbarcelona.eu)
  • X-ray structure of a hydroxylase-regulatory protein complex from a hydrocarbon-oxidizing multicomponent monooxygenase, Pseudomonas sp. (anl.gov)
  • Like those in other diiron carboxylate proteins, such as methane monooxygenase (MMO), the R2 diiron cluster is proposed to activate oxygen by formation of a peroxodiiron(III) intermediate followed by an oxidizing high-valent cluster. (psu.edu)
  • Whereas kinetic and spectroscopic studies of the reaction have generally been conducted at pH 7.6 with reactant produced by the addition of Fe(II) ions to the apo protein, the available crystal structures of diferrous R2 have been obtained by chemical or photoreduction of the oxidized diiron(III) protein at pH 5-6. (proteopedia.org)
  • Variable coordination geometries at the diiron(II) active site of ribonucleotide reductase R2. (proteopedia.org)
  • All known RNRs used during aerobic metabolism require a metal ion cofactor, which acts as a powerful oxidizing agent to drive the conversion. (futurity.org)