• Laccases (EC 1.10.3.2) are multicopper oxidases found in plants, fungi, and bacteria. (wikipedia.org)
  • Laccases (benzendiol: oxygen oxidoreductase, EC 1.10.3.2) are belong to polyphenol oxidase, catalyze the reduction of dioxygen to water while oxidize its substrates ( Baldrian, 2006 ). (scialert.net)
  • The laccase (EC 1.10.3.2) from Trametes versicolor was purchased from Fluka (specific activity 0.53 U mg -1 ) and was used without further purification. (scialert.net)
  • The smaller polypeptide of M(r) ~73 kDa yielded an N-terminal amino-acid sequence that was homologous to laccase-like polyphenol oxidases (E.C. 1.10.3.2) from loblolly pine (Pinus taeda L.), poplar (Populus euramericana (Dode) Guinier) and Arabidopsis. (who.int)
  • Laccase (EC 1.10.3.2 ) (urishiol oxidase), an enzyme found in fungi and plants, which oxidizes many different types of phenols and diamines. (expasy.org)
  • Other laccases, such as those produced by the fungus Pleurotus ostreatus, play a role in the degradation of lignin, and can therefore be classed as lignin-modifying enzymes. (wikipedia.org)
  • However, thus far only a limited number of laccase or laccase -like enzymes identified through metagenomics has been subsequently biochemically characterized. (uninsubria.it)
  • This work describes the successful bio-mining of bacterial laccase-like enzymes in an acidic bog soil metagenome and the characterization of the first acidobacterial laccase-like multicopper oxidase (LMCO). (uninsubria.it)
  • With this source laccases are one of enzymes with high amount of literatures could be observed. (scialert.net)
  • These unique properties made laccases to be promising enzymes for bioremediation. (scialert.net)
  • However, many substrates of laccases are not oxidized due to the enzymes have relatively low redox potentials. (scialert.net)
  • Designer laccases: a vogue for high-potential fungal enzymes? (google.co.uk)
  • These biosensors are complexes of graphene oxide and biomacromolecules, including enzymes such as glucose oxidase, horseradish peroxidase, laccase, and nucleic acids such as DNA and RNA. (intechopen.com)
  • This phytopathogen has an abundance of secreted oxidases, peroxidases, and hydrolytic enzymes for degrading cell wall polysaccharides and lignocelluloses to penetrate into the host tissue. (biomedcentral.com)
  • Comparing soluble and co-immobilized catalysts for 2-ketoaldose production by pyranose 2-oxidase and auxiliary enzymes. (boku.ac.at)
  • The ability of bacterial laccases to be generated both intracellularly and extracellularly, with active enzymes across a broad pH and temperature range, was investigated. (biomedcentral.com)
  • Have been clarified some issues such as about synthesis of hydrolase and oxidase enzymes by some of the mushroom and regulation of synthesis in selected mushroom as an active producer. (scirp.org)
  • Taking this into account, the presented work was devoted to the evaluation of xylotroph macromycetes spread in the nature of Azerbaijan by the activity of enzymes such as phenol oxidase, more accurate laccase, peroxidase, and lignin. (scirp.org)
  • This chromosomal region contains two laccase genes (HvLAC1 and HvLAC2) that are highly expressed at the seedling stage and may encode enzymes that oxidize pCA during the formation of HA. (bvsalud.org)
  • Molecular docking was adopted to predict the catalytic interaction of chosen toxic diazo colorant, Congo red (CR), with four dye-degrading enzymes (laccase, lignin peroxidase, azoreductase, and aryl alcohol oxidase). (bvsalud.org)
  • In-depth investigations through preliminary bioinformatic analyses and enzymatic assays on the supernatant of R7 grown in the presence of PE confirmed the activation of genes encoding laccase-like enzymes. (unimib.it)
  • Laccases oxidize a variety of phenolic substrates, performing one-electron oxidations, leading to crosslinking. (wikipedia.org)
  • Bilirubin oxidase (BOD) is capable of oxidizing laccase substrates. (sigmaaldrich.com)
  • Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)
  • Using molecular oxygen as an electron acceptor, laccase oxidizes its substrates by an electron transfer mechanism that generates unstable free radical intermediates and causes non-enzymatic reactions that break down substrate molecules [ 4 ]. (biomedcentral.com)
  • Laccase substrate specificity differs from one organism to another, and in the presence of adequate redox mediators, the range of laccase oxidizable substrates can be greatly extended [ 5 ]. (biomedcentral.com)
  • Studies utilizing fungal and bacterial laccases to degrade emerging pollutants have also been conducted. (wikipedia.org)
  • The known fungal producers of laccase are deuteromycetes, ascomycetes, and basidiomycetes. (biomedcentral.com)
  • Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. (sigmaaldrich.com)
  • Regioselective Control of beta-D-Glucose Oxidation by Pyranose 2-Oxidase Is Intimately Coupled to Conformational Degeneracy. (boku.ac.at)
  • Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration. (boku.ac.at)
  • Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
  • Geszvain, K., McCarthy, J.K. and Tebo, B.M. Elimination of manganese(II,III) oxidation in Pseudomonas putida GB-1 by a double knockout of two putative multicopper oxidase genes. (enzyme-database.org)
  • In the laccase-ABTS mediator system, the best molar ratio of dye/mediator was 1:10 and dye/enzyme ratio was 0.5 μmol U -1 . (scialert.net)
  • This enzyme firstly found in Japanese lacquer tree, Rhus vernicifera and not long after that laccases from fungi has been reported, especially in white-rot fungi. (scialert.net)
  • Crystal structure of the 270 kDa homotetrameric lignin-degrading enzyme pyranose 2-oxidase. (boku.ac.at)
  • L-ascorbate oxidase (EC 1.10.3.3 ), a higher plant enzyme. (expasy.org)
  • The enzyme, which belongs to the multicopper oxidase family, is found in many bacterial strains. (enzyme-database.org)
  • Pyranose oxidase: A versatile sugar oxidoreductase for bioelectrochemical applications. (boku.ac.at)
  • H-Bonding and Positive Charge at the N(5)/O(4) Locus Are Critical for Covalent Flavin Attachment in Trametes Pyranose 2-Oxidase. (boku.ac.at)
  • ** Halada, P., Leitner, C., Volc, J., Haltrich, D., Havlicek, V. Peptide sequencing by matrix-assisted laser desorption/ionization and post-source decay mass spectroscopy: a rapid method to design oligonucleotide hybridisation probes for cloning cDNA encoding of pyranose 2-oxidase from Trametes multicolor. (boku.ac.at)
  • To explore new bioresources for mycoremediation, a high laccase-producing novel white-rot fungus (WRF), Trametes flavida WTFP2, was employed. (bvsalud.org)
  • In this study, we investigated the expression profile of laccase (POXA3) and ß-glucan synthase (FKS) genes during different developmental stages in two strains of P. ostreatus. (bvsalud.org)
  • putative laccase, a member of laccase family of genes (17 members in Arabidopsis). (riken.jp)
  • Other laccases produced by fungi can facilitate the biosynthesis of melanin pigments. (wikipedia.org)
  • Laccase is found in a wide range of higher plants, insects, bacteria, and mainly in fungi. (biomedcentral.com)
  • Basidiomycetes, particularly white rot fungi, are thought to be effective laccase producers among them. (biomedcentral.com)
  • electrophoretic analysis of coniferyl alcohol oxidase and related laccases. (liverpool.ac.uk)
  • gradient gel electrophoretic methods enabled a distinction to be made between coniferyl alcohol oxidase (cao) of lignifying cell walls and a pi approximately 9 pine 'laccase' recently implicated in lignification (science 1993 260, 672). (liverpool.ac.uk)
  • in the following order: A-A-B-A-C-C. The A-type domain is related to the multicopper oxidases. (expasy.org)
  • For example, laccases play a role in the formation of lignin by promoting the oxidative coupling of monolignols, a family of naturally occurring phenols. (wikipedia.org)
  • Cholesterol Oxidase from Streptomyces sp. (sigmaaldrich.com)
  • Biochemical studies of the multicopper oxidase (small laccase) from Streptomyces coelicolor using bioactive phytochemicals and site-directed mutagenesis. (gc.ca)
  • Other instances of bacteria that generate laccases comprise Streptomyces antibioticus, Pseudomonas putida , Campylobacter jejuni , E. coli , Bacillus spp. (biomedcentral.com)
  • The type I copper center consists of a single copper atom that is ligated to a minimum of two histidine residues and a single cysteine residue, but in some laccases produced by certain plants and bacteria, the type I copper center contains an additional methionine ligand. (wikipedia.org)
  • The type III copper can be replaced by Hg(II), which causes a decrease in laccase activity. (wikipedia.org)
  • Using molecular screening of a small metagenomic library (13,500 clones), a gene encoding a three-domain LMCO (LacM) was detected, showing the highest similarity to putative copper oxidases of Candidatus Solibacter (Acidobacteria). (uninsubria.it)
  • Bilirubin Oxidase is composed of three cupredoxin-like domains that result in two active sites formed by four copper ions. (sigmaaldrich.com)
  • Structurally ceruloplasmin exhibits internal sequence homology, and seem to have evolved from the triplication of a copper-binding domain similar to that found in laccase and ascorbate oxidase. (expasy.org)
  • Both patterns are derived from the same region, which in ascorbate oxidase, laccase, in the third domain of ceruloplasmin, and in copA, contains five residues that are known to be involved in the binding of copper centers. (expasy.org)
  • Laccase is a copper-containing polyphenol oxidase capable of oxidising polyphenols, methoxy-containing substituted phenols, diamines and a number of other compounds. (vtrbiotech.com)
  • Due to its high capability in binding metals, it reduces the activity of oxidases (tyrosinase and laccase) by blocking copper. (enartis.com)
  • Bilirubin oxidase (BOD) belongs to the subclass of the multicopper oxidase family. (sigmaaldrich.com)
  • Bilirubin Oxidase from Myrothecium verrucaria has been used to eliminate the interference of bilirubin. (sigmaaldrich.com)
  • Bilirubin oxidase is used to degrade bilirubin. (sigmaaldrich.com)
  • Here we will show bioelectrocatalytic behaviour of earlier proposed electrodes modified with adsorbed bilirubin oxidase from Myrothecium sp. (science24.com)
  • Francis, C.A., Casciotti, K.L. and Tebo, B.M. Localization of Mn(II)-oxidizing activity and the putative multicopper oxidase, MnxG, to the exosporium of the marine Bacillus sp. (enzyme-database.org)
  • For instance, laccase from Bacillus subtilis is most active at 75 °C, and at 80 °C, it has a 4 h half-life [ 11 ]. (biomedcentral.com)
  • Interestingly, one of the identified potential host interactors of smut_5844 was laccase-10 protein (OsLAC10), which has been predicted to be involved in plant lignification and iron metabolism. (biomedcentral.com)
  • In researches, were evaluated 103 strains of 25 species of xylotrophic micromycetes causing white decay which spreads in ecologically different regions of Azerbaijan for the synthesis of phenoloxidases (laccase, peroxidase, and ligninase). (scirp.org)
  • Two laccase isoenzymes (LacA and LacB) were isolated from a novel Trichoderma harzianum S7113 isolate employing ammonium sulfate precipitation, Sephadex G100, and DEAE Sepharose ion exchange chromatography. (biomedcentral.com)
  • Laccases catalyze ring cleavage of aromatic compounds. (wikipedia.org)
  • In addition, MCO and laccase will be applied in combination with silica or other metal oxide-forming proteins (recombinant silicatein and silintaphin-1) to generate nanoparticles containing multiple shells of various materials, which can be doped with fluorescent dyes and proteins during their formation at mild conditions. (irb.hr)
  • Omics approaches for identification of gene candidate(s) encoding extracellular laccase like multicopper oxidase from Psychrobacter sp. (sintef.no)
  • Laccases efficiency could be enhanced when the low molecular weight compounds are included as redox mediators. (scialert.net)
  • Researchers in China have now reported a nanowire-based biofuel cell based on a single proton conductive polymer nanowire for converting chemical energy from biofluids into electricity, using glucose oxidase and laccase as catalyst. (nanowerk.com)
  • Laccases have the potential to crosslink food polymers such as proteins and nonstarch polysaccharides in dough. (wikipedia.org)
  • Although laccase is known to crosslink AX, under the microscope it was found that the laccase also acted on the flour proteins. (wikipedia.org)
  • The ability of laccases to degrade various aromatic polymers has led to research into their potential for bioremediation and other industrial applications. (wikipedia.org)
  • Our findings indicate that purified laccase has a promising capacity for bisphenol A (BPA) bioremediation across a broad pH range. (biomedcentral.com)
  • Our group shown that the nanoparticulate film electrode prepared by layer by layer method combined with sol-gel process exhibits mediated bioelectrocatalysis with laccase dissolved in electrolyte. (science24.com)
  • Laccases affects the oxygen reduction reaction at low overpotentials. (wikipedia.org)
  • However, the reactions catalyze by LiP or MnP need hydrogen peroxide as co-substrate, this substance is not necessary for laccases catalysis. (scialert.net)
  • In all species where oxidase activity was detected histochemically, it was expressed in cell walls of lignifying, differentiating xylem cells and was absent from old wood, cambium and phloem, suggesting that oxidases have a conservative role in lignification of tree xylem. (who.int)
  • and gymnosperms (Ginkgo biloba L.), oxidase activity was associated with cell walls of developing xylem and was enriched in extracts of cell wall-associated glycoproteins. (who.int)
  • A portion of the total oxidase activity had high affinity for immobilized zinc ions and this feature allowed it to be separated from the bulk of oxidase activity. (who.int)
  • Two polypeptides that could have been responsible for the bound oxidase activity were enriched by this procedure. (who.int)
  • In particular, it has been shown that laccases can be applied to catalyze the degradation and detoxification a large range of aromatic contaminants, including azo dyes, bisphenol A and pharmaceuticals. (wikipedia.org)
  • In non-starch polysaccharides, such as arabinoxylans (AX), laccase catalyzes the oxidative gelation of feruloylated arabinoxylans by dimerization of their ferulic esters. (wikipedia.org)
  • Laccase is a glycosylated monomer with a molecular mass ranging from 54 to 97 kDa, relying on the source species. (biomedcentral.com)
  • Laccase is also able to oxidize peptide-bound tyrosine, but very poorly. (wikipedia.org)
  • Laccase was first studied by Hikorokuro Yoshida in 1883 and then by Gabriel Bertrand in 1894 in the sap of the Japanese lacquer tree, where it helps to form lacquer, hence the name laccase. (wikipedia.org)
  • Rhus vernicifera , a Japanese lacquer tree, is where laccase was initially discovered. (biomedcentral.com)