In particular, the p16/cyclin-dependent kinase inhibitor 2A (CDKN2A) gene located on chromosomal region 9p21 frequently is altered in several types of cancer. (iiarjournals.org)
The tumor suppressor gene p16/cyclin-dependent kinase inhibitor-2A (CDKN2A) is located within the chromosomal region 9p21 and encodes a cell-cycle protein that is an inhibitor of cyclin-dependent kinases (CDK)-4 and -6. (iiarjournals.org)
Mice lacking the tumor suppressors p16(Ink4a) (Cdkn2a, cyclin-dependent kinase inhibitor 2a), p19(Arf) (an alternative reading frame product of Cdkn2a,), and p27(Kip1) (Cdkn1b, cyclin-dependent kinase inhibitor 1b) result in malignant progression of epithelial cancers, sarcomas, and melanomas, respectively. (koreamed.org)
Although the senescent cells remain viable, they show typical changes with enlarged and flattened cell bodies, apoptosis resistance, increased activity of senescence-associated β -galactosidase (SA- β -gal), and upregulation of cyclin-dependent kinase (CDK) inhibitors including p16 INK4A , ARF proteins, and p21 [ 13 - 16 ]. (hindawi.com)
Defects of the retinoblastoma protein (pRb)/cyclin-D1/p16 pathway have been shown to play a critical role in the development of human malignancies. (iiarjournals.org)
A series of 76 cases of gastrointestinal tract endocrine tumors, including 21 poorly differentiated endocrine carcinomas (PDECs, small cell carcinomas) and 55 well-differentiated endocrine neoplasms (WDENs, carcinoids), 18 metastatic and 37 nonmetastatic rectal carcinoids, were examined by immunohistochemical analysis for p16 INK4a , cyclin D1, and retinoblastoma protein (pRB) expression. (nycu.edu.tw)
9. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Immunohistochemical overexpression of p16 protein associated with intact retinoblastoma protein expression in cervical cancer and cervical intraepithelial neoplasia. (journal-imab-bg.org)
Utility of p161
A comparison of the clinical utility of p16(INK4a) immunolocalization with the presence of human papillomavirus by hybrid capture 2 for the detection of cervical dysplasia/neoplasia. (journal-imab-bg.org)
CDKN2A1
A number of studies have shown that p16/CDKN2A is frequently altered in melanoma ( 9 ), pancreatic ( 10 ), urinary bladder ( 11 ) and lung cancer ( 12 ). (iiarjournals.org)
Genes8
Promoter methylation of p16 INK4A , c-myc and hMSH2 genes was assayed by methylation-specific PCR (MSP) and sequencing (mapping). (wjgnet.com)
The transcriptional levels of p16 INK4A and MeCP2 genes did not display any difference between gastric cancerous and matched non-cancerous tissues. (wjgnet.com)
Common secondary abnormalities include mutations in TP53, RB, and p16/INK4A, or overexpression of genes such as EVI1. (genome.jp)
Previous studies have shown the involvement of cell-autonomous factors in this phenomenon, particularly the decline of polycomb genes and accumulation of p16/INK4A. (diabetesjournals.org)
The lentivirus-mediated overexpression of psoriasin, mimicking the psoriatic milieu, gave rise to an altered regulation of differentiation genes and an expression pattern reminiscent of that in psoriatic epidermis. (medicaljournals.se)
P16INK4A and P19ARF (P14ARF in humans) are two tumor suppressor genes encoded by INK4a-ARF and inhibited by Bmi-1. (docksci.com)
Furthermore, the cell cycle regulatory genes p16 INK4a and p14 ARF are noted to be altered in lesions of proliferative verrucous leukoplakia. (medscape.com)
Conclusão: Os resultados suportam a investigação revelando que os genes MIG, CK14, p63, Cal A e Cys A se apresentam fortemente evidentes nos tumores de cavidade oral e o p16 suprimido, sugerindo que esta proteína pode exercer um papel de regulador negativo do ciclo celular. (bvsalud.org)
Immunohistochemical3
In situ hybridization (ISH) assays for high-risk human papillomavirus (HR-HPV) and immunohistochemical (IHC) assays for surrogate markers such as p16 INK 4a can be useful in detecting HR-HPV in cervical dysplasia. (journal-imab-bg.org)
Karcheva M, Popovska S, Nachev R. Immunohistochemical investigations of P16 INK 4a expression in carcinomas and high grade cervical lesions. (journal-imab-bg.org)
7. Negri G1, Egarter-Vigl E, Kasal A, Romano F, Haitel A, Mian C. p16INK4a is a useful marker for the diagnosis of adenocarcinoma of the cervix uteri and its precursors: an immunohistochemical study with immunocytochemical correlations. (journal-imab-bg.org)
Tumor2
The aim of the present study was to determine the significance of this tumor suppressor gene for ovarian tumorigenesis, investigating both numerical aberrations of chromosome 9 and p16 gene alterations in 28 cases of ovarian tumors, by the fluorescence in situ hybridization (FISH) technique. (iiarjournals.org)
The aim of the following study was to find difference the level for expression of the tumor specific gene p16 INK 4a in reactive, dysplastic and invasive changes in biopsy specimens from uterine cervix. (journal-imab-bg.org)
Cervical2
8. Sano T, Oyama T, Kashiwabara K, Fukuda T, Nakajima T. Expression status of p16 protein is associated with human papillomavirus oncogenic potential in cervical and genital lesions. (journal-imab-bg.org)
Although p16 INK4a has been well established as sensitive and accurate diagnostic biomarker for endocervical adenocarcinoma in situ and invasive adenocarcinoma in histologic sections and in cervical cytology specimens, clinical and diagnostic features alone have limited power to predict the survival of patients with endocervical adenocarcinoma. (medscape.com)
Proteins3
In contrast overexpression of PcG proteins correlates with the severity and invasiveness of several cancer types. (wikipedia.org)
Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)
Telomerase is excessively activated in most of tumors and is involved in the regulation of cell cycle proteins, such as p16Ink4a and p21WAF1, which induce epithelial cell apoptosis [7]. (docksci.com)
Methylation2
Loss of function of the p16 gene has been reported to occur mainly by homozygous deletion, mutation or aberrant DNA methylation of the promoter of the region ( 5 - 8 ). (iiarjournals.org)
Overexpression of MBD4 inhibited CD70 expression and enhanced the DNA methylation level of CD70 in CD4 + T cells of SLE patients. (biomedcentral.com)
Alterations2
Materials and Methods: To investigate both the presence of numerical abnormalities of chromosome 9 and p16 gene alterations in ovarian cancer, we studied 28 cases by the fluorescence in situ hybridization (FISH) technique using a DNA p16 probe and an a-satellite probe specific for chromosome 9. (iiarjournals.org)
Frequent alterations of p16INK4a and p14ARF in oral proliferative verrucous leukoplakia. (medscape.com)
Analyses1
E and F) Western blot analyses for p16 in FVB (E) and B6 (F) mouse embryonic fibroblasts. (koreamed.org)
Carcinoma1
Objetivo: O objetivo deste estudo foi avaliar, através de iomunohistoquímica, a expressão dos marcadores protéicos MIB, CK14, p63, p16, Cal A e Cys A em lesões bucais com diagnóstico histopatológico de carcinoma espinocelular. (bvsalud.org)
Biomarker2
Immunocytochemical staining for p16, a proven biomarker for high grade dysplasia associated with Human Papillomavirus (HPV) infection, has been previously applied successfully to cervicovaginal cytology specimens 4 . (cytojournal.com)
Based on the similar pathogenesis of squamous and glandular lesions of the cervix, we hypothesized that K17 overexpression could also be a diagnostic and/or prognostic biomarker for endocervical neoplasia. (medscape.com)
Specimens2
We included p16 based upon recent published data by our group indicating thatTrichomonas vaginalisorganisms in cervicovaginal specimens are immunoreactive for p16 3 . (cytojournal.com)
However, p16 immunoreactivity is not specific for HPV-infected epithelium, as immunoreactivity has previously been documented with inflammatory cells, multinucleated giant cells, bacteria and mucus in cervicovaginal specimens [ 4 - 6 ]. (cytojournal.com)
Invasive2
Almost 100% of the high grade lesions and the invasive carcinomas demonstrated high level of expression of p16 INK 4a while non dysplastic epithelium is always negative. (journal-imab-bg.org)
It absolutely was witnessed in which 87.9% of the patients along with invasive patch confirmed overexpression involving p16(INK4a), in comparison to Thirty-seven.6% of those with in situ patch (X-2: Tough luck. (survivinsignaling.com)
Protein1
Conclusion: p63, p16, MIB, Cal A, Cys A are markedly expressed and p16 is strongly suppressed in oral cavity tumors, which suggests that the latter protein may play a role in negative regulation of cell cycle progression. (bvsalud.org)
Regulatory1
The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
Lesions2
In the lesions of the uterine cervix, over expression of p16 INK 4a is induced by HPV and is associated with the process of cancerogenesis in the epithelium. (journal-imab-bg.org)
Role of P16(INK4a) expression in identifying CIN2 or more severe lesions among HPV-positive patients referred for colposcopy after abnormal cytology. (journal-imab-bg.org)
Chromosome2
Conclusion: Numerical aberrations of chromosome 9 and p16 gene deletion are common findings in ovarian cancer. (iiarjournals.org)
Data suggest that the p16 gene, located in the short arms of chromosome 9, may play a role in ovarian carcinogenesis. (iiarjournals.org)
Transcription3
Transcription levels of Dnmt1, mbd2, MeCP2, p16 INK4A , hMSH2 and c-myc were detected by using real-time PCR or RT-PCR. (wjgnet.com)
The p16(Ink4a) and p19(Arf) knockout mice were generated via transcription activator-like effector nucleases (TALENs), and p27(Kip1) knockout mice via clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9 (CRISPR/Cas9). (koreamed.org)
A and B) Representative views of the transcription activator-like effector nuclease (TALEN) targeting strategy for generation of p16Ink4a KO mice in both FVB (A) and B6 (B) strains. (koreamed.org)
Expression2
The expression of p16/INK4A mRNA did not change in heterochronic parabiosis, suggesting the involvement of other pathways. (diabetesjournals.org)
The p16 INK4a /cyclin D1/pRB pathway was altered in gastrointestinal tract endocrine tumors, and the loss of expression of pRB may be helpful in identifying patients at high risk of metastasis in rectal WDENs. (nycu.edu.tw)
Diagnostic1
Based on this finding, we recommend p16 immunocytochemistry as a diagnostic tool for unexpected patients or specimen types in which potential trichomonads are identified following routine cytologic evaluation. (cytojournal.com)
There are few studies focusing on the molecular analysis of p16 gene in ovarian cancer ( 13 - 16 ). (iiarjournals.org)
Human1
In order to differentiate parasites from degenerated urothelial cells in our case we prepared additional ThinPrep[TM] slides from the residual specimen to perform immunocytochemistry for cytokeratin (using a cocktail of high- and low-molecular weight keratins) and p16 (using primary purified mouse anti-human p16 antibody, clone G175-405, supplied by BD Biosciences Pharmingen, San Diego, CA, USA). (cytojournal.com)
Psoriasin (S100A7) was first identified for its overexpression in psoriatic keratinocytes (3), where it was found to be located to the nucleus and the cytoplasm (4). (medicaljournals.se)
High1
In this system very high levels of E 2 F 1 overexpression cause. (wikigenes.org)
Mouse1
Figure 1 TALEN-mediated generation of a p16Ink4a knockout mouse model. (koreamed.org)
Strong1
vaginaliswere p16 positive and demonstrated strong cytoplasmic staining. (cytojournal.com)