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Aurora KinasesAurora Kinase BAurora Kinase AAurora Kinase CProtein-Serine-Threonine KinasesMitosisChromosome SegregationSpindle ApparatusKinetochoresOrganophosphatesProtein Kinase InhibitorsMicrotubule-Associated ProteinsPhosphorylationCell Cycle ProteinsI-kappa B KinaseCytokinesisChromosomal Proteins, Non-HistoneProtein KinasesHeLa CellsCentromerePhosphatidylinositol 3-KinasesMAP Kinase Signaling SystemMicrotubulesM Phase Cell Cycle CheckpointsPolyploidyCell Line, TumorXenopus ProteinsCalcium-Calmodulin-Dependent Protein KinasesAnaphasesrc-Family KinasesCell CycleQuinazolinesApoptosisProtein BindingCDC2 Protein KinaseKinesinRNA InterferenceHistonesMutationSpisulaProtein Kinase CMolecular Sequence DataAmino Acid Sequencep38 Mitogen-Activated Protein KinasesNuclear ProteinsCell ProliferationCyclic AMP-Dependent Protein KinasesInhibitor of Apoptosis ProteinsEnzyme ActivationCell LineSignal TransductionMitogen-Activated Protein Kinase 1MetaphaseJNK Mitogen-Activated Protein KinasesProtein-Tyrosine KinasesIntracellular Signaling Peptides and ProteinsCentrosomeChromosomes, HumanChromosomesTransfectionRNA, Small Interferingp21-Activated KinasesBenzamidesMitogen-Activated Protein Kinase KinasesBlotting, WesternCyclin-Dependent KinasesAntineoplastic AgentsMultiprotein ComplexesMitogen-Activated Protein Kinase 3Cell DivisionPiperazinesRecombinant Fusion ProteinsModels, BiologicalProto-Oncogene ProteinsAzepinesEnzyme InhibitorsMAP Kinase Kinase KinasesProtein Phosphatase 1Creatine KinaseCaenorhabditis elegans ProteinsChromatidsProtein TransportCasein Kinase IIeIF-2 KinaseSaccharomyces cerevisiae ProteinsMicroscopy, FluorescenceCasein KinasesRibosomal Protein S6 KinasesPyrimidinesPyruvate KinaseRNA, MessengerMitogen-Activated Protein KinasesProtein Structure, TertiaryCaenorhabditis elegansMAP Kinase Kinase 1IsoenzymesGene Expression Regulation, EnzymologicReceptor Protein-Tyrosine KinasesCell Cycle CheckpointsCells, Cultured
PhosphorylationSerineCytokinesisMitotic kinaseInvolved in centrosomeCentrosome amplificationMitosisInhibitionReceptorBindsChromosomal passenger proteinLocalizationUbiquitinationTargetsMalignantLocalizesRegulationTumorRegulatesTherapeuticOrganismsActivityPhosphorylatesProteinsSpindleMicrotubuleChromosomeActivationRoleSurvivalCancerPhaseResultsExpressionCell linesStudiesLocationAssemblyHumanForm
Phosphorylation8
  • Localization of Aurora B to the centromere during prometaphase and metaphase requires phosphorylation of the mammalian kinetochore-specific histone-H3 variant centromere protein A (CENP-A). CENP-A associates with the centromere and is necessary for assembly of the kinetochore. (wikipedia.org)
  • Phosphorylation of CENP-A at serine 7 by Aurora A kinase recruits Aurora B to the centromere. (wikipedia.org)
  • We herein demonstrated that CADM1 interacts with Hippo pathway core kinases and enhances the phosphorylation of YAP1, and also that the membranous co-expression of CADM1 and LATS2 predicts a favorable prognosis in lung adenocarcinoma. (cancerindex.org)
  • The increased centrosomal localization of BRCA1 and Aurora A induced by CDDP treatment involved the nuclear export of BRCA1 and BRCA1 phosphorylation by ataxia telangiectasia mutated (ATM). (bvsalud.org)
  • Patient-derived variants and mutations at phosphorylated residues of BRCA1 suppressed the interaction between BRCA1 and Aurora A, as well as the CDDP-induced increase in the centrosomal localization of BRCA1 and Aurora A. These results suggest that CDDP induces the phosphorylation of BRCA1 by ATM in the nucleus and its transport to the cytoplasm, thereby promoting the centrosomal localization Aurora A, which phosphorylates PLK1. (bvsalud.org)
  • The increase of Rsk in STHdh Q111/Q111 cells occurred in the cytosol and in the nucleus, which results in enhanced phosphorylation of both cytosolic and nuclear Rsk targets. (biomedcentral.com)
  • Modifications Inhibitors,Modulators,Libraries in the phosphorylation state of the DAT by kinases causes alterations in the function and location of the DAT. (inhibitorkit.com)
  • At present, it remains poorly understood how DDK docks onto the helicase and how the kinase targets distal Mcm subunits for phosphorylation. (imperial.ac.uk)
Serine6
  • The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
  • A family of highly conserved serine-threonine kinases that are involved in the regulation of MITOSIS. (lookformedical.com)
  • An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. (lookformedical.com)
  • Mammalian aurora-A belongs to a multigenic family of mitotic serine/threonine kinases comprising two other members: aurora-B and aurora-C. In this review we will focus on aurora-A that starts to localize to centrosomes only in S phase as soon as centrioles have been duplicated, the protein is then degraded in early G1. (lookformedical.com)
  • A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. (lookformedical.com)
  • The 90-kDa ribosomal S6 kinase (Rsk) is a family constituted by four isoforms (Rsk1-4) of serine/threonine kinases broadly expressed in the brain that regulate important cellular functions, including cell survival [ 1 ]. (biomedcentral.com)
Cytokinesis3
  • Indirect immunofluorescence showed that Aurora B and EB1 colocalize during anaphase on the central spindle and in the midbody during cytokinesis. (wikipedia.org)
  • Treating SCCHN cell lines with a pan-Aurora kinase inhibitor resulted in defective cytokinesis, polyploidy and apoptosis, which was effective irrespective of the EGFR status. (oncotarget.com)
  • Chemical genetics reveals the requirement for Polo-like kinase 1 activity in positioning RhoA and triggering cytokinesis in human cells. (famri.org)
Mitotic kinase1
  • In this study, we show the role of the mitotic kinase Aurora A in the reduction of centrosomal OLA1. (bvsalud.org)
Involved in centrosome3
  • An aurora kinase that localizes to the CENTROSOME during MITOSIS and is involved in centrosome regulation and formation of the MITOTIC SPINDLE. (lookformedical.com)
  • Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)
  • Here, the centrosomal localization of BRCA1 and the kinases involved in centrosome duplication were analyzed in each cell cycle phase after treatment with DNA crosslinker cisplatin (CDDP). (bvsalud.org)
Centrosome amplification2
Mitosis7
  • Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. (wikipedia.org)
  • Expression of Aurora B reaches a maximum at the G2-M transition, whereas Aurora B protein is most active during mitosis. (wikipedia.org)
  • This analysis showed that the association of Aurora B with centromeres is dynamic (Aurora B at the centromere is constantly exchanging with a pool of cytoplasmic Aurora B). The analysis of tagged Aurora B also suggested that it associates with spindle microtubules during anaphase of mitosis and this association significantly limits its mobility. (wikipedia.org)
  • In topoisomerase II-depleted cells, Aurora B and INCENP do not transfer to the central spindle in late mitosis. (wikipedia.org)
  • Aurora kinase C is a chromosomal passenger protein that interacts with aurora kinase B in the regulation of MITOSIS. (lookformedical.com)
  • An aurora kinase that is a component of the chromosomal passenger protein complex and is involved in the regulation of MITOSIS. (lookformedical.com)
  • Aurora-A phosphorylates and modulates the power of RASSF1A to associate with microtubule during mitosis (17). (healthandwellnesssource.org)
Inhibition5
  • Inhibition of Aurora B due to loss of topoisomerase II seems to depend on BubR1 activity (see below). (wikipedia.org)
  • Inhibition of Aurora B function by RNA interference or microinjection of blocking antibodies impairs the alignment of chromosomes at the equator of the mitotic spindle. (wikipedia.org)
  • Aurora B inhibition may lead to an increase in the number of syntelic attachments (sister chromatid pairs in which both sister kinetochores are attached to microtubules radiating from the same spindle pole). (wikipedia.org)
  • Combined Aurora kinase and EGFR targeting using a monoclonal anti-EGFR antibody was more effective compared to single EGFR and Aurora kinase inhibition. (oncotarget.com)
  • Finally, pharmacological inhibition of Rsk, knock-down and overexpression experiments indicated that Rsk activity exerts a protective effect against mhtt-induced cell death in STHdh Q7/Q7 cells transfected with mhtt. (biomedcentral.com)
Receptor4
Binds3
  • The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. (cancerindex.org)
  • The zinc finger domain of Tzfp binds to the tbs motif located at the upstream flanking region of the Aie1 (aurora-C) kinase gene. (lookformedical.com)
  • Aurora A binds to and polyubiquitinates OLA1, targeting it for proteasomal degradation. (bvsalud.org)
Chromosomal passenger protein1
Localization4
  • A more detailed analysis of Aurora B localization has been carried out in mammalian cells by tagging Aurora B with green fluorescent protein. (wikipedia.org)
  • Additionally, topoisomerase II has been implicated in the regulation of Aurora B localization and enzymatic activity. (wikipedia.org)
  • BRCA1 contributed to the increased centrosomal localization of Aurora A in S phase and that of phosphorylated Polo-like kinase 1 (PLK1) in late S phase after CDDP treatment, resulting in centriole disengagement and overduplication. (bvsalud.org)
  • Amphetamine, a psychostim ulant, also causes reversal and altered cellular location of the DAT which is known to be regulated by kinases, phos phatases, and Ca2 localization and association. (inhibitorkit.com)
Ubiquitination1
  • whereas overexpression of CUL4A and DDB1 enhances RASSF1A proteins ubiquitination leading to reduced RASSF1A amounts markedly. (healthandwellnesssource.org)
Targets1
Malignant2
  • Aurora A overexpression in many malignant tumor types suggests that it may be directly involved in NEOPLASTIC CELL TRANSFORMATION. (lookformedical.com)
  • Adusumilli PS, Chan MK, Hezel M, Yu Z, Stiles BM, Chou TC, Rusch VW, Fong Y. Radiation-induced cellular DNA damage repair response enhances viral gene therapy efficacy in the treatment of malignant pleural mesothelioma. (famri.org)
Localizes2
Regulation2
  • The results suggest a dual function of Aurora A as an E3 ubiquitin ligase and a kinase in the regulation of centrosomal OLA1, which is essential for proper centrosome maturation in G2 phase. (bvsalud.org)
  • Interestingly, previous data from a HD cellular model show a de-regulation of both kinases that control Rsk activity. (biomedcentral.com)
Tumor1
  • Patients characterized by elevated EGFR and elevated Aurora-A protein expression in tumor tissue represent a risk group with poor disease-free and overall survival (EGFR low Aurora-A low versus EGFR high Aurora-A high , p=0.024). (oncotarget.com)
Regulates1
  • Aurora B has been shown to bind to end-binding protein 1 (EB1), a protein that regulates microtubule dynamics. (wikipedia.org)
Therapeutic1
Organisms2
Activity6
  • The expression and activity of Aurora B are regulated according to the cell cycle. (wikipedia.org)
  • INCENP stimulates Aurora B kinase activity. (wikipedia.org)
  • Also, cells deficient in topoisomerase II show significantly reduced Aurora B kinase activity. (wikipedia.org)
  • Intriguingly, EB1 overexpression enhances Aurora B kinase activity, at least in part because EB1 blocks the dephosphorylation/inactivation of Aurora B by protein phosphatase 2A. (wikipedia.org)
  • The kinase activity of Aurora A suppresses OLA1 polyubiquitination. (bvsalud.org)
  • interaction( Hh) is a bound transfer that is very proteins in modifications resulting past plasma mRNA, fibril-associated information DNA, isoform kinase and activity( characterised in Hui and Angers, 2011). (evakoch.com)
Phosphorylates1
  • NIMA-related kinase 2 (NEK2) phosphorylates the T124 residue of OLA1, increases binding of OLA1 to Aurora A and OLA1 polyubiquitination by Aurora A, and reduces centrosomal OLA1 in G2 phase. (bvsalud.org)
Proteins2
  • Aurora B complexes with three other proteins, Survivin, Borealin and INCENP. (wikipedia.org)
  • The decrease in centrosomal OLA1 caused by Aurora A-mediated polyubiquitination promotes the recruitment of pericentriolar material proteins in G2 phase. (bvsalud.org)
Spindle1
  • Aurora kinase B is a protein that functions in the attachment of the mitotic spindle to the centromere. (wikipedia.org)
Microtubule1
  • Intriguingly, expression of a dominant-negative and catalytically inactive form of Aurora B disrupted microtubule attachment to the kinetochore and prevented the association of dynein and centromere protein E (CENP-E) with kinetochores. (wikipedia.org)
Chromosome1
Activation3
  • RACK1 is involved in centriole duplication in the S phase by promoting polo-like kinase 1 activation by Aurora A, which is critical for centrosome duplication. (bvsalud.org)
  • Rsk activation is regulated by sequential phosphorylations controlled by extracellular signal-regulated kinase (ERK) 1/2 and 3-phosphoinositide-dependent protein kinase 1 (PDK1). (biomedcentral.com)
  • This creates an environment for increased rapid bioavail ability of E2 which can elicit nongenomic effects such as Ca2 mobilization, kinase activation, and alterations in dopamine subcellular location via membrane estrogen receptors. (inhibitorkit.com)
Role1
Survival1
Cancer1
  • Bae KM, Wang H, Jiang G, Chen MG, Lu L, Xiao L. Protein kinase C epsilon is overexpressed in primary human non-small cell lung cancers and functionally required for proliferation of non-small cell lung cancer cells in a p21/Cip1-dependent manner. (famri.org)
Phase1
  • We also remember that overexpression of CUL4A antagonizes the power of RASSF1A to induce M-phase cell routine arrest. (healthandwellnesssource.org)
Results1
  • Since that time several RASSF1A kinases are also discovered including cyclin-dependent kinase-4 (18) PKC (19) MST1 (macrophage stimulating-1) (20) and Aurora-B (21) that have different results on RASSF1A function. (healthandwellnesssource.org)
Expression1
  • Comparing pan-Aurora kinase and Aurora-A targeting hints towards a strong and clinically relevant biological effect mediated via Aurora kinase B. Taken together, our findings characterize a new poor risk group in SCCHN patients defined by elevated EGFR and Aurora-A protein expression. (oncotarget.com)
Cell lines1
  • However, overexpression of aurora-A in particular cell lines such as NIH3T3 is sufficient to induce growth on soft agar. (lookformedical.com)
Studies1
Location1
  • Finally, a portion of the tagged Aurora B localized to the equatorial cell cortex, having been transported to this location by astral microtubules. (wikipedia.org)
Assembly1
  • The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. (imperial.ac.uk)
Human1
  • In human, aurora-A has focused a lot of attention, since its overexpression has been found to be correlated with the grade of various solid tumours. (lookformedical.com)
Form1
  • Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene. (lookformedical.com)