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  • meiotic arrest
  • Many studies have shown that cyclic adenosine-5-monophosphate (cAMP)-dependent protein kinase A (PKA) and G-protein-coupled receptor 3 (GPR3) are crucial for controlling meiotic arrest in oocytes. (jove.com)
  • retrieval
  • Therefore, it can be used to promote the start of oocyte maturation, when given as a trigger prior to oocyte retrieval. (cochrane.org)
  • This review focuses on the administration of human chorionic gonadotrophin (hCG) prior to immature oocyte retrieval. (cochrane.org)
  • For each cycle, men whose female partner was undergoing IVF or IUI provided one spot urine sample at the time of oocyte retrieval or insemination, respectively. (thefreedictionary.com)
  • From a medical standpoint, this case of postmortem oocyte retrieval is fraught with difficulty. (thefreedictionary.com)
  • Additionally, it avoids ethical concerns regarding ovarian stimulation and oocyte retrieval in children. (thefreedictionary.com)
  • The drops were prepared in Falcon #3002 Petri dishes (Becton-Dickinson Labware, Lincoln Park, NJ) one to three hours prior to oocyte retrieval with media that were equilibrated overnight in a 36. (thefreedictionary.com)
  • Women who underwent oocyte retrieval at our institution between August 1995 and February 1997 and agreed to participate in the study were enrolled consecutively. (thefreedictionary.com)
  • Table 1 gives an overview of the mean number of days of stimulation, the mean of the total number of ampoules of FSH used during the stimulation, the mean of the final E2 concentrations before oocyte retrieval , and the mean of the daily percent E2 increase. (thefreedictionary.com)
  • Maturation in vitro of human oocytes from unstimulated cycles: selection of the optimal day for ovum retrieval based on follicular size. (springer.com)
  • stimulation
  • Most GJC between cumulus cells and oocyte ceased immediately after FSH stimulation and recommenced after the cAMP surge. (jove.com)
  • Tregs can be generated from naive CD4 T cells in vitro with TCR- and co-stimulation in the presence of TGFβ and IL-2. (jove.com)
  • Assisted reproduction usually involves ovarian stimulation to obtain a higher yield (or number) of oocytes (immature egg cells). (cochrane.org)
  • The major benefit of IVM is that significant numbers of oocytes can be collected from ovaries without recourse to ovarian stimulation. (whsmith.co.uk)
  • Although initially limited to women with polycystic ovary syndrome who were at higher risk of problems following ovarian stimulation, IVM is increasingly used in women with normal ovulatory menstrual cycles. (whsmith.co.uk)
  • Exogenous gonadotropin stimulation for short courses seems to improve the ultrastructure of the oocytes expected to mature in in vitro conditions. (tjoddergisi.org)
  • phosphorylation
  • PKAI activation inhibited mitogen-activated protein kinase (MAPK) phosphorylation in the oocytes of COCs, which participated in the initiation of FSH-induced meiotic maturation in vitro. (jove.com)
  • Just before FSH-induced meiotic maturation, cAMP, PKAI, and GPR3 returned to basal levels, and PDE3A activity and MAPK phosphorylation increased markedly. (jove.com)
  • Recombinant tUCHL1 absorbed p34(cdc2), a component of MPF, in obviously larger quantities from mature oocytes than from immature oocytes, and p13(suc1) was isolated from tUCHL1 with a dependence on the ATP regeneration system, suggesting that still other functions may be involved in their association that require phosphorylation. (jove.com)
  • donor
  • Oocytes are manually defolliculated from donor ovary tissue, injected or treated in culture as desired, and then stimulated with progesterone to induce maturation. (jove.com)
  • theca cells
  • From this tissue, they extracted the cells which would form the foundations for their artificial ovaries, called theca cells. (healthbo.com)
  • mechanism
  • In oocytes from hibernation-interrupted toads, the p34(cdc2) protein level was significantly lower than in oocytes from toads in artificial hibernation, providing an explanation for the different quantities isolated by recombinant tUCHL1 pull-down and, more importantly, identifying a mechanism involved in the toad oocytes dependence on a low environmental temperature during winter hibernation. (jove.com)
  • Mechanism of oocyte maturation. (whsmith.co.uk)
  • unclear
  • However, it is unclear how gonadotropins modulate these factors to regulate oocyte maturation, especially by gap junctional communication (GJC). (jove.com)
  • tissue
  • Neonatal myocytes are a common cell source used for in vitro studies in cardiac tissue formation and engineering 4 . (jove.com)
  • The researchers first collected human ovarian tissue from women who had had their ovaries removed for reasons other than cancer. (healthbo.com)
  • ovarian hypersti
  • Although hMGs increased the number of oocytes and the chance of pregnancy, they brought about Ovarian Hyperstimulation syndrome (OHSS), which could even be fatal for an otherwise healthy young woman (16) . (tjoddergisi.org)
  • donors
  • Study I investigated the influence of age and reproductive status of female donors on the number and quality of oocytes. (vin.com)
  • a larger number of oocytes were recovered at interestrus/anestrus when only adult donors were considered (p=0.0075). (vin.com)
  • (17) reported the first IVM birth from immature oocytes derived from oocyte donors. (tjoddergisi.org)
  • reproductive
  • A significant association was observed between the reproductive phases and oocyte quality (p=0.002). (vin.com)
  • In vitro maturation (IVM) is one of the most controversial aspects of assisted reproductive technology. (tjoddergisi.org)
  • Oocyte collection is a highly specialized assisted reproductive technique, which requires technical expertise and specialized equipment. (vetstream.com)
  • PCOS affects the ovaries but since it is an endocrine disease, it has an impact on the entire hormonal balance and can lead to various metabolic, cardiovascular, reproductive and general complications. (mediologiest.com)
  • assays
  • Computational tools, expression and proteomics assays, and chromatin-immunoprecipitation-based techniques provide important clues for identifying mRNAs that are direct targets of a particular miRNA. (jove.com)
  • novel
  • The simulated ovary is a novel, versatile tool which can be used in the planning phase of experiments to estimate the expected number of animals and workload, ensuring appropriate statistical power of the resulting measurements. (jove.com)
  • We have applied a powerful and novel well-tempered ensemble parallel tempering (PT-WTE) technique along with conventional molecular dynamics (MD) simulation to investigate the molecular-level consequences of selective methionine oxidation of fibrinogen. (jove.com)
  • Racowsky, C. A novel system for in vitro maturation of human oocytes. (springer.com)
  • development
  • this allows one to combine genetic or pharmacologic perturbations with in vitro development to define the effects of such manipulations on the processes occurring during specific periods of development. (jove.com)
  • Our observations provide potential explanation for the effects of Glut on follicular development in rat ovary and a role for eCG in the regulation of ovarian glucose uptake. (jove.com)
  • Atresia remains the major form of follicular expenditure at all stages since development of ovary. (jove.com)
  • 0,0001), showing that puberty influences oocyte capacity for in vitro development. (vin.com)
  • This book is about the development, biology and pathology of the oocyte. (leedsmedics.org.uk)
  • Cells
  • During FSH-induced maturation, rapid cAMP surges were observed in both cumulus cells and oocyte. (jove.com)
  • The soft agar colony formation assay is a well-established method for characterizing this capability in vitro and is considered to be one of the most stringent tests for malignant transformation in cells. (jove.com)
  • Our aim was to develop and characterize a highly reproducible rat syngeneic in vitro model of the BBB using co-cultures of primary rat brain endothelial cells (RBEC) and astrocytes to study receptors involved in transcytosis across the endothelial cell monolayer. (jove.com)
  • The researchers set out to create a three-dimensional artificial structure that could act as an "artificial ovary" and mature human oocytes (immature egg cells). (healthbo.com)
  • These granulosa cells came from oocytes obtained in IVF procedures. (healthbo.com)
  • characteristics
  • We developed computer algorithms, which generate models of neonatal mouse ovaries (simulated ovaries), with characteristics derived from experimental measurements already available in the published literature. (jove.com)
  • Histological evaluation in both groups showed that morphological characteristics of the ovaries were the same. (vin.com)
  • pregnancy
  • In case of pregnancy, the rat would have estrous reaction and "blut punkte" (blood points) in the ovary, determined by a laparotomy approximately 100 h after the injection. (ispub.com)
  • The process we want to concentrate on here is auto-grafting: Once a patient is cleared for pregnancy, auto-grafting involves re-implanting surgically little strips of ovarian cortex where the ovary used to be. (centerforhumanreprod.com)
  • If successful transplantation is accomplished, this technique, therefore, potentially offers a woman multiple chances of achieving pregnancy. (centerforhumanreprod.com)
  • gene
  • Additionally, many of the techniques to overexpress or inhibit gene function in organisms such as Xenopus and zebrafish fail to sufficiently target critical maternal signaling pathways, such as Wnt signaling. (jove.com)
  • In Xenopus , manipulating gene function in cultured oocytes and subsequently fertilizing them can ameliorate these problems to some extent. (jove.com)
  • Because generation of transgenic and gene-targeted mouse mutants is costly and can take long periods of time, manipulation of female gametes in vitro is a more economical and time-saving strategy. (jove.com)
  • Gene expression during oocyte maturation. (whsmith.co.uk)
  • alginate
  • Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. (jove.com)
  • experiments
  • the proposed approach provides the necessary number of ovaries and sampling frequency to be used in the experiments given a specific biological variability and a desirable degree of accuracy. (jove.com)
  • Previous antibody-blocking experiments have demonstrated that toad ubiquitin carboxyl-terminal hydrolase L1 (tUCHL1) is necessary for germinal vesicle breakdown during toad oocyte maturation. (jove.com)
  • In the context of MPF (maturation promoting factor) controlling oocyte maturation and to further understand the role of UCHL1 in oocyte maturation, we performed adsorption and co-immunoprecipitation experiments using toad oocyte protein extracts and determined that tUCHL1 is associated with MPF in toad oocytes. (jove.com)