• Youssef M, De Sanctis JB, Kanagaratham C, Tao S, Ahmed E, Radzioch D. Efficacy of Optimized Treatment Protocol Using LAU-7b Formulation against Ovalbumin (OVA) and House Dust Mite (HDM) -Induced Allergic Asthma in Atopic Hyperresponsive A/J Mice. (harvard.edu)
  • A Chlamydia trachomatis Strain Expressing Ovalbumin Stimulates an Antigen-Specific CD4+ T Cell Response in Mice. (harvard.edu)
  • Mice were sensitized with s.c. injection of ovalbumin (OVA) on days 1 and 8. (frontiersin.org)
  • All mice strains were systemically sensitized and exposed to filtered air or ovalbumin (OVA) aerosol for two weeks to create a subchronic model of allergen-induced airway inflammation. (hindawi.com)
  • In a previous study, we have shown several allergic and metabolic alterations after 7 days of ovalbumin (OVA) ingestion by sensitized mice. (wiley.com)
  • Splenocytes from mice sensitized with ovalbumin (OVA) were isolated for analyses of cytokine production and cell viability. (mdpi.com)
  • Alternaria extract, when administered to mice intranasally with ovalbumin (OVA) protein, induces a rapid (1-48 h) innate response while also priming an OVA-specific Th2 response that can be evoked 14 days later by intranasal administration of OVA alone. (nih.gov)
  • Shibayagi's OVA-IgE ELISA KIT is a useful tool for studying mouse immune system by measuring specific anti-OVA IgE after immunization of mice with OVA (ovalbumin). (biovendor.com)
  • Intradermal immunization with ovalbumin-loaded poly-epsilon-caprolactone microparticles conferred protection in ovalbumin-sensitized allergic mice. (cun.es)
  • Baseline lung resistance and methacholine induced AHR were measured in mice sensitised to ovalbumin (OVA) and also in mice infected with respiratory syncytial virus (RSV) following ovalbumin sensitisation (OVA/RSV). (bmj.com)
  • Gloriosaol isomeric mixtures (GLM) are isolated from the extract, structurally confirmed FIA-MS/MS. Both YGE and GLM are evaluated against ovalbumin-induced airway hyperresponsiveness (AHR) in Balb/C mice. (omicsonline.org)
  • In this study, we investigated the acute oral toxicity of an Insampaedok-san water extract (ISSE) in rats and the antiasthmatic effects of ISSE and its mechanism in a model of asthma induced by ovalbumin (OVA) in mice. (biomedcentral.com)
  • The plasticizer di-(2-ethylhexyl) phthalate (DEHP) has been shown to stimulate a non-allergy related immune response with increased levels of IgG1 and IgG2a, but not IgE, after co-administration with the model allergen ovalbumin (OVA) in mice. (biomedcentral.com)
  • IgE, IgG and lymph node T cell responses in mice were studied using ovalbumin mixed with topical transdermal cream formulations containing a potential immune modulator applied to the skin. (longdom.org)
  • BALB/c mice were immunized with Ovalbumin (OVA) mixed with alum to induce an IgE response. (longdom.org)
  • Mice developed sIgE to ovalbumin after 14 days and application of cream formulations showed a dose dependent reduction in IgE compared to controls at 42 and 70 days. (longdom.org)
  • Therefore, it was hypothesized that the topical application of an allergen contained in a transdermal base cream would produce Anti-Ovalbumin (OVA) antibody production in mice. (longdom.org)
  • All mice received a single injection of 2 nanograms (ng) chicken Ovalbumin (OVA) mixed with alum intraperitoneally (i.p.) on day 0 in order to stimulate an immune IgG and IgE response. (longdom.org)
  • Cytotoxic T lymphocytes (CTL) from the ovalbumin-hsp70-immunized mice and a highly effective CTL clone (4G3) raised against ovalbumin-expressing EL4 tumor cells (EG7-OVA) were equally effective in terms of the concentration of this compound required for half-maximal lysis in a CTL assay. (bachem.com)
  • Ovalbumin-treated mice were used as an asthma model. (elsevier.com)
  • FL- or GM-CSF-treated mice injected with soluble ovalbumin display dramatic increases in antigen-specific antibody titers, but the isotype profiles seem critically dependent on the cytokine used. (pnas.org)
  • Xiong J, Liu S, Pan Y, Zhang B, Chen X and Fan L: Combination of fish oil and ethanol extracts from Spirulina platensis inhibits the airway inflammation induced by ovalbumin in mice. (spandidos-publications.com)
  • Alloferon remarkably decreased the number of eosinophils, macrophages, and neutrophils in the bronchoalveolar lavage fluid (BALF) from ovalbumin (OVA)-induced asthma mice. (bvsalud.org)
  • Methods: BALB/c mice were sensitized to ovalbumin (OVA) and challenged with one or three OVA aerosols. (elsevier.com)
  • Turner, D. J. / Ovalbumin-sensitized mice are good models for airway hyperresponsiveness but not acute physiological responses to allergen inhalation . (elsevier.com)
  • In this study, we investigated the HMT effects on ovalbumin-induced asthmatic in mice. (jikm.or.kr)
  • The acute allergen challenge involves repeated exposure to ovalbumin for a period of four or five weeks in mice or two or three weeks in rats (several protocols available, depending on endpoints). (fraunhofer.de)
  • Allergic asthma was induced in BALB/c mice by sensitization and challenge with ovalbumin (OVA). (ac.ir)
  • Ovalbumin (257-264) is an antigenic peptide fragment of ovalbumin that contains a CD8+ cytotoxic T-cell (CTL)-specific epitope with the sequence 'SIINFEKL. (lktlabs.com)
  • Dissection of major histocompatibility complex (MHC) and T cell receptor contact residues in a Kb-restricted ovalbumin peptide and an assessment of the predictive power of MHC-binding motifs. (lktlabs.com)
  • SIINFEKL, an ovalbumin-derived peptide, is known to arise from natural processing of ovalbumin H-2b mouse cells. (bachem.com)
  • From measurements of the displacement of the precipitin curve of irradiated ovalbumins from the position of the curve for native ovalbumin the loss of activity due to primary ionizations was determined. (jimmunol.org)
  • Solutions of the γ-irradiated ovalbumins were fractionated by promptly precipitating the radiation-denatured molecules at the isoelectric point of native ovalbumin, and by heating the neutral systems at 50°, 60° and 68°C and by precipitating the thermolabile constituents at the isoelectric point. (jimmunol.org)
  • A particular set of biopolymer materials was employed: native ovalbumin (OVA), OVA nanosized aggregate (OVAn) and high methoxyl pectin (HMP). (gob.ar)
  • We also investigated the anti-asthmatic effects of SE in an ovalbumin (OVA)-induced BALB/c mouse model. (biomedcentral.com)
  • Molecular mechanism of chicken ovalbumin upstream promoter-transcription factor (COUP-TF) actions" (PDF). (wikipedia.org)
  • Several studies have demonstrated interaction of these factors with chicken ovalbumin upstream promoter transcription factor II (Coup-TFII). (nih.gov)
  • studies of serpin structure and function (the fact that ovalbumin does not inhibit proteases means that by comparing its structure with that of inhibitory serpins, the structural characteristics required for inhibition can be determined). (wikipedia.org)
  • Aim: To determine the efficacy of murine model of ovalbumin (OVA)-induced AHR inhibition by morin and decipher the molecular mechanism involved. (eurekaselect.com)
  • Dose-dependent thiol and immune responses to ovalbumin challenge in Brown Norway rats. (cdc.gov)
  • Adjuvant effect of aqueous extract of Rhodiola imbricata rhizome on the immune responses to tetanus toxoid and ovalbumin in rats. (semanticscholar.org)
  • Actively immunised Brown Norway rats received ovalbumin inhalation with or without treatment of anti-MIF Ab. (elsevier.com)
  • An immunohistochemical study revealed positive immunostaining for MIF in bronchial epithelium, even in nonsensitised rats, and the MIF staining in bronchial epithelium was enhanced after the ovalbumin challenge. (elsevier.com)
  • CB2 Agonist (AM1241) Improving Effect on Ovalbumin-Induced Asthma in Rats', Iranian Journal of Pharmaceutical Research , 19(1), pp. 3-17. (ac.ir)
  • In order to better understand the role of the cannabinoid (CB) 2 receptor in the ovalbumin (OVA)-induced asthma model, a combination of both selective CB2 agonist (AM1241) and antagonist (AM630) was used to improve inflammatory hypersensitivity and edema in rats. (ac.ir)
  • In sensitized rats, three intratracheal instillations of ovalbumin resulted in EGF-R expression and goblet-cell production in airway epithelium. (pnas.org)
  • We show that stimulation of EGF-R by its ligands results in mucin-producing goblet cells and that ovalbumin sensitization in rats causes induction of EGF-R and goblet-cell production in airways. (pnas.org)
  • The aim of this work was to evaluate the properties of poly-epsilon-caprolactone (PCL) microparticles as adjuvants in immunotherapy using ovalbumin (OVA) as an allergen model. (cun.es)
  • Characterization of the steroid-dependent regulatory element (SDRE) in the check ovalbumin gene (-892 to -796) suggests that it functions as a hormone response unit. (ebscohost.com)
  • Transfection experiments using linker scanning and point mutations support the contention that the binding of these three complexes is essential for induction of the ovalbumin gene by steroid hormones. (ebscohost.com)
  • Highly purified hen oviduct progesterone receptor A subunit has been found to interact preferentially with the 5'-flanking region of the chicken ovalbumin gene. (springer.com)
  • Saturation kinetics showed that 2-fold less receptor was required for half-maximal binding to ovalbumin gene flanking sequences than to plasmid pBR322 sequences. (springer.com)
  • M. LeMeur, N. Glanville, J. L. Mandel, P. Gerlinger, R. Palmiter, P. Chambon, The ovalbumin gene family: Hormonal control of X and Y gene transcription and mRNA accumulation. (springer.com)
  • G. E. Swaneck, J. L. Nordstrom, F. Kruezaler, M. J. Tsai, O'Malley, The effect of estrogen on gene expression in the chicken oviduct: Evidence for transcriptional control of ovalbumin gene. (springer.com)
  • The expression of the ovalbumin gene in hen oviduct decreases during ageing. (mysciencework.com)
  • The ovalbumin gene. (scripps.edu)
  • Purified ovalbumin messenger RNA was employed to selectively enrich the concentration of the gene coding for ovalbumin from total chick DNA by molecular hybridization. (scripps.edu)
  • The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. (scripps.edu)
  • Since ovalbumin mRNA has a complexity of 1890 nucleotides, the resulting DNA was more than twice the length of ovalbumin mRNA and thus should contain DNA sequences adjacent to the structural portion of the ovalbumin gene. (scripps.edu)
  • Pulmonary dendritic cell distribution and prevalence in guinea pig airways: effect of ovalbumin sensitization and challenge. (cdc.gov)
  • Four weeks after sensitization with ovalbumin (0.1 mg x k(-1)), the guinea-pigs were anaesthetized and mechanically ventilated. (ersjournals.com)
  • This study aims to evaluate the effect of KOB03 on ovalbumin (OVA)-induced allergic rhinitis (AR) in guinea pigs. (springer.com)
  • Our previous study demonstrated that a complex traditional Chinese medicine (CTCM) administered in nebulized form through the intratracheal route is effective against early-phase air-flow obstruction and can inhibit IL-5 production in ovalbumin (OVA)-sensitized guinea pigs. (biomedcentral.com)
  • Northern transfer of electrophoretically fractionated total RNA from oviducts of mature egg-laying and old non-egg-laying hens, and hybridization to nick-translated plasmid pOV230 (ovalbumin complementary DNA cloned into pMB9) revealed with age increasing concentrations of distinct high-molecular-weight ovalbumin pre-mRNA species as compared with that of functional 18S ovalbumin mRNA. (mysciencework.com)
  • The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. (scripps.edu)
  • When apparent Cot1/2 values of these reactions were compared to the apparent Cot1/2 value obtained from the hybridization reaction between 125I-ovalbumin mRNA and complementary DNA synthesized against ovalbumin mRNA using the enzyme reverse transcriptase, purification of the coding ovalbumin DNA strand over total chick DNA was estimated to be approximately 9,600-fold. (scripps.edu)
  • Translation of ovalbumin mRNA in Xenopus laevis oocytes. (elsevier.com)
  • Ovalbumin messenger RNA (mRNA(ov)) purified from hen oviduct was injected into Xenopus laevis oocytes. (elsevier.com)
  • Injection of increasing amounts of mRNA(ov) was found to elicit a linear response in terms of ovalbumin synthesis. (elsevier.com)
  • Ovalbumin mRNA activity was present in RNA preparations from chicks treated with estrogen but was undetectable in animals withdrawn from the hormone. (elsevier.com)
  • Thermo Scientific™ Imject Maleimide-Activated Ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens. (fishersci.ca)
  • Although FL treatment induces up to a 10,000-fold increase in ovalbumin-specific IgG2a and a more modest increase in IgG1 titers, GM-CSF treatment favors a predominantly IgG1 response with little increase in IgG2a levels. (pnas.org)
  • The purpose of the present study was to determine the effects of orally administered bromelain in an ovalbumin (OVA)-induced murine model of acute allergic airway disease (AAD). (pubmedcentralcanada.ca)
  • This study aimed to evaluate the preventive activity of IMP in an ovalbumin (OVA)-induced murine model of asthma and to investigate its possible molecular mechanisms. (ovid.com)
  • In addition, we examined the antioxidant activity of ISS and its antiasthmatic effects by using a mouse model of asthma induced by ovalbumin (OVA) in vivo to investigate the mechanism underlying the effectiveness of ISS in the treatment of asthma. (biomedcentral.com)
  • BUF048 is a preparation of HRP conjugated ovalbumin, which may be used for the measurement of ovalbumin specific IgE in immunoassay systems, without species restriction. (bio-rad-antibodies.com)
  • The crystal structure of uncleaved, hen ovalbumin was solved by the molecular replacement method using the structure of plakalbumin, a proteolytically cleaved form of ovalbumin, as a starting model. (uniprot.org)
  • The final refined model, including four ovalbumin molecules, 678 water molecules and a single metal ion, has a crystallographic R-factor of 17.4% for all reflections between 6.0 and 1.95 A resolution. (uniprot.org)
  • Secondary structure of OVAX is similar to that of ovalbumin, but the three-dimensional model of OVAX reveals the presence of a cluster of exposed positive charges, which potentially explains the affinity of this ov-serpin for heparin, as opposed to ovalbumin. (sigmaaldrich.com)
  • Experimental animal models, such as the ovalbumin (OVA)-induced model of allergic airway disease (AAD), are utilized to investigate the underlying mechanisms and potential therapies for asthma. (pubmedcentralcanada.ca)
  • To explore the potential pharmaceutical effects of Lactobacillus rhamnosus GG (LGG) in treating allergic rhinitis in a rat model sensitized by ovalbumin (OVA). (alliedacademies.org)
  • Therefore, HMT might have an anti-asthmatic effect in the ovalbumin-induced mouse model. (jikm.or.kr)
  • Ovalbumin-induced acute allergic asthma in mouse or rat is a very well-characterized model, which can be used to study immunologic and inflammatory pathomechanisms. (fraunhofer.de)
  • Purified, carrier-grade ovalbumin has been modified with SMCC crosslinker to attach maleimide groups capable of forming covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other molecules. (fishersci.ca)
  • Stoichiometric, kinetic and thermodynamic aspects of complex formation between heat-induced aggregates of ovalbumin (ovalbumin nanoparticles, OVAn) and linoleic acid (LA) were evaluated. (gob.ar)
  • Ovalbumin is a member of serpin superfamily, but it does not have any proteinase inhibitor activity. (nii.ac.jp)
  • Altogether, these results suggest that OVAX and ovalbumin, although highly similar in sequence, have peculiar sequential and/or structural features that are likely to impact their respective biological functions. (sigmaaldrich.com)
  • The accumulation of ovalbumin sequence-containing processing intermediates in old hen oviducts indicates lowered rates of RNA splicing and/or altered steady state concentrations of splicing intermediates during post-transcriptional maturation of heterogeneous nuclear RNA in old animals. (mysciencework.com)