• Furthermore, in AD brains, mitochondrially associated APP formed stable ∼480 kDa complexes with the translocase of the outer mitochondrial membrane 40 (TOM40) import channel and a super complex of ∼620 kDa with both mitochondrial TOM40 and the translocase of the inner mitochondrial membrane 23 (TIM23) import channel TIM23 in an "N in mitochondria -C out cytoplasm " orientation. (jneurosci.org)
  • Solution NMR-based characterization of the structure of the outer mitochondrial membrane protein Tom40 and a novel method for NMR resonance assignment of large intrinsically disordered proteins. (mpg.de)
  • Within the cell, TSPO is predominantly located in mitochondria, specifically in the outer mitochondrial membrane. (uni-regensburg.de)
  • Members of this group include: PagC, required by Salmonella typhimurium for survival in macrophages and for virulence in mice Rck outer membrane protein of the S. typhimurium and S. enteritidis virulence plasmid Ail, a product of the Yersinia enterocolitica chromosome capable of mediating bacterial adherence to and invasion of epithelial cell lines OmpX from Escherichia coli that promotes adhesion to and entry into mammalian cells. (wikipedia.org)
  • Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli. (wikigenes.org)
  • Modulations in lipid A and phospholipid biosynthesis pathways influence outer membrane protein assembly in Escherichia coli K-12. (wikigenes.org)
  • Based on the Escherichia coli OmpA model, an eight-stranded amphipathic beta-barrel model for the membrane assembly of the N-terminal half of OmpAI and OmpAII was predicted. (shengsci.com)
  • An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. (shengsci.com)
  • 2006. Genetic characterization of Escherichia coli O157: H7-strains carrying the stx2 gene but not producing Shiga toxin 2. (ipb.ac.id)
  • Escherichia coli DegS is an integral inner membrane protein that breaks down incorrectly assembled proteins and helps regulate the σ ᴱ stress response pathway. (emerginginvestigators.org)
  • The repressor role of SepR was established by conducting tests with a sep-lacZ transcriptional fusion in Escherichia coli and PpF1, expression of SepR as a maltose-binding fusion protein in a DNA binding assay, and mRNA analysis. (canada.ca)
  • We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
  • The method harnesses the intrinsic intracellular folding quality control mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway to display an scFv library on the E. coli inner membrane. (jove.com)
  • In this publication, we characterize a novel outer membrane protein of Moraxella catarrhalis which exists in two variant forms associated with particular genetic lineages, and both forms are suggested to contribute to bacterial clearance from the lungs. (eur.nl)
  • Virulence-related outer membrane proteins, or outer surface proteins (Osp) in some contexts, are expressed in the outer membrane of gram-negative bacteria and are essential to bacterial survival within macrophages and for eukaryotic cell invasion. (wikipedia.org)
  • This family consists of several bacterial and phage Ail/Lom-like proteins. (wikipedia.org)
  • Lom is found in the bacterial outer membrane, and is homologous to virulence proteins of two other enterobacterial genera. (wikipedia.org)
  • This protein disappears from the bacterial surface around 2 weeks after infection. (wikipedia.org)
  • Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. (bmj.com)
  • Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
  • Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis. (bmj.com)
  • In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)
  • This is the first report of any bacterial species possessing two genes for homologs of this major outer membrane protein. (shengsci.com)
  • To date, only one bacterial factor has been shown to mediate intestinal adherence by O157:H7, the outer membrane protein intimin which was originally discovered in the Principal Investigator's laboratory. (usda.gov)
  • The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial virulence and viability. (biomedcentral.com)
  • The deduced amino acid sequences encoded by sepABC show a high homology to bacterial proteins known to be involved in solvent efflux or multidrug pumps. (canada.ca)
  • Further analysis indicated that these two proteins possessed almost identical gene sequences, apart from two insertion/deletion events in predicted external loops present within the putative barrel-like structure of the proteins. (eur.nl)
  • encodes a putative lipoprotein, designated outer membrane protein P4 (OmpP4). (biomedcentral.com)
  • In this study the expression of an outer membrane component of a putative drug efflux system of B. burgdorferi was shown for the first time. (uni-wuerzburg.de)
  • RePub, Erasmus University Repository: Identification and characterization of a novel outer membrane protein (OMPJ) of Moraxella catarrhalis that exists in two major forms. (eur.nl)
  • Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis. (nih.gov)
  • Identification and characterisation of antigens and outer membrane proteins of the periodontopathic bacteria P. gingivalis , T. denticola and B. forsythus . (edu.au)
  • In the first part of this study outer membrane preparations of three Borrelia relapsing fever strains have been studied for pore-forming activity in the black lipid bilayer assay. (uni-wuerzburg.de)
  • In the second part, for the B. burgdorferi HB19 and p66 knock out strain HB19/K02, their outer membrane preparations were characterized in the black lipid bilayer assay. (uni-wuerzburg.de)
  • The purified mutant proteins were reconstituted into lipid bilayer membranes and the single-channel conductance was studied. (uni-wuerzburg.de)
  • Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis. (wikigenes.org)
  • Four lipid molecules are located within or above an inner-membrane funnel formed by Tic214, Tic20, Simp1 and Ctap5. (nature.com)
  • Fig. 4: Arrangement of protein subunits and lipid molecules in the inner-membrane complex. (nature.com)
  • We characterize the lipid and protein components in intact stratum corneum using natural abundance PT ssNMR, scattering and sorption techniques. (lu.se)
  • Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
  • Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
  • We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)
  • The protein identified was FrpB3, its respective gene was overexpressed with haemoglobin. (walshmedicalmedia.com)
  • Expression of ompAI and ompAII separately in E. coli indicated that both genes could be independently transcribed from their own promoters and that both gene products were assembled into the E. coli outer membrane. (shengsci.com)
  • The sepR gene encodes a 260-residue polypeptide that is a member of the E. coli IclR repressor protein family. (canada.ca)
  • Amplification of the meningococcal porB gene for non-culture serotype characterization. (ox.ac.uk)
  • The NDP gene provides instructions for making a protein called norrin. (medlineplus.gov)
  • NDP gene mutations that cause this condition affect the ability of the norrin protein to bind with frizzled-4, interfering with the specialization of retinal cells for their unique sensory function. (medlineplus.gov)
  • HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
  • Characterization of three fimbrial genes, sefABC, of Salmonella enteritidis. (wikigenes.org)
  • In this study, we describe the isolation and characterization of nanobodies (Nb) recognizing multiple Campylobacter strains. (biomedcentral.com)
  • Some strains produce a protein that confers resistance to complement by interfering with the formation of the membrane attack complex. (medscape.com)
  • Although OmpX has the same beta-sheet topology as the structurally related outer membrane protein A (OmpA) InterPro: IPR000498, their barrels differ with respect to the shear numbers and internal hydrogen-bonding networks. (wikipedia.org)
  • Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. (shengsci.com)
  • The encoded proteins (Mr = 33,564 and 32,536 for mature OmpAI and OmpAII, respectively) had only 64% identity with each other and otherwise had the highest level of homology to OmpA proteins from the members of the family Enterobacteriaceae. (shengsci.com)
  • Characterization of virulence of Leptospira isolates in a hamster model. (fiocruz.br)
  • The most potent compound, darobactin B, was used for co-crystallization with the target BamA, revealing an identical binding site to darobactin A. Besides its potency, darobactin B did not exhibit cytotoxicity and was slightly more active against Acinetobacter baumanii isolates than darobactin A. Furthermore, we evaluated the plasma protein binding of darobactin A and B, indicating their different pharmacokinetic properties. (sdsc.edu)
  • One such lipoprotein, OmpP4, is homologous to the outer membrane lipoprotein e (P4) of H. influenzae . (biomedcentral.com)
  • pneumophila), and subsequently performed a detailed characterisation of the dynamics and impact of homologous recombination. (pacb.com)
  • We identified genomic "hotspots" of homologous recombination that include regions containing outer membrane proteins, the lipopolysaccharide (LPS) region and Dot/Icm effectors, which provide interesting clues to the selection pressures faced by L. pneumophila. (pacb.com)
  • Moreover, these loops form a protruding beta sheet, the edge of which presumably binds to external proteins. (wikipedia.org)
  • The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans. (fiocruz.br)
  • Specifically, FHA binds directly to the ciliary membrane host-receptor glycospingolipids, allowing "B. bronchiseptica" to attach to the host's cells. (kenyon.edu)
  • Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
  • Genetic and Molecular Regulation of Seed Storage Proteins (SSPs) to Improve Protein Nutritional Value of Oilseed Rape (Brassica napus L.) Seeds. (uni-bielefeld.de)
  • Molecular cloning, characterization and three-dimensional structure prediction of Lipoxygenase from Finger millet [Eleusine coracana (L.) Gaertn. (molquest.com)
  • We aim at a characterization of the material properties of the SC by coupling macroscopic SC barrier properties to its molecular structure and dynamics. (lu.se)
  • Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts. (nature.com)
  • Genetic and structural characterization of the human mitochondrial inner membrane translocase. (nih.gov)
  • Mounting evidence suggests that the genetic disorders/mutation and diseases change not only the protein expression patterns but also membranes themselves. (lu.se)
  • In the present study, we explored the structural aspect of HomA and HomB proteins using various computational, biophysical and small-angle X-ray scattering (SAXS) techniques. (nature.com)
  • Our study provides essential structural information of unexplored proteins of the Hom family that can help in a better understanding of H. pylori pathogenesis. (nature.com)
  • Cleavage of structural proteins during the assembly of the head of bacteriophage T4. (microbiologyresearch.org)
  • Since, karilysin has been already characterized, in this study we propose to study about mirolysin through structural, biochemical and biological characterization. (bepress.com)
  • This is a protein known to fold extremely well in the periplasm of E. Coli. (igem.org)
  • A range of proteins (n = 20) previously associated with the mar locus in E. coli were also found including the key MAR effectors AcrA, TolC and OmpF. (reading.ac.uk)
  • Proteins in this family are predicted to consist of eight transmembrane beta-sheets and four cell surface-exposed loops. (wikipedia.org)
  • Most variation between the two proteins was localized to the predicted surface loops and periplasmic turns, while the transmembrane strands and C-terminals domains were highly conserved. (shengsci.com)
  • Predicted to enable protein transmembrane transporter activity. (nih.gov)
  • This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)
  • During studies to investigate the outer membrane proteins of this bacterium, two novel major proteins, of approximately 19 kDa and 16 kDa (named OMP J1 and OMP J2, respectively), were identified. (eur.nl)
  • This bacterium synthesizes three membrane proteins of 48, 50 and 77 kDa capable of binding haem, unfortunately their identities remains undetermined [ 17 ]. (walshmedicalmedia.com)
  • Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)
  • The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)
  • Following removal of the outer membrane, the scFvs displayed on the inner membrane are panned against a target antigen immobilized on magnetic beads to isolate scFvs that bind to the target antigen. (jove.com)
  • Borrelia species are able to induce cycles of disease by varying antigen expression and by displaying new outer-surface proteins during the disease course. (medscape.com)
  • NLPE is an outer membrane lipoprotein that literature has shown actibates the cpX pathway. (igem.org)
  • SepA, SepB and SepC are referred to be periplasmic, inner membrane and outer membrane efflux proteins respectively. (canada.ca)
  • Sigma factors are a class of proteins constituting essential dissociable subunits that confer sequence-specific DNA binding properties to RNA polymerase (RNAP). (hindawi.com)
  • Accumulation of APP across mitochondrial import channels, which varied with the severity of AD, inhibited the entry of nuclear-encoded cytochrome c oxidase subunits IV and Vb proteins, which was associated with decreased cytochrome c oxidase activity and increased levels of H 2 O 2 . (jneurosci.org)
  • The major subunits of the TOC complex (Toc75, Toc90 and Toc34) and TIC complex (Tic214, Tic20, Tic100 and Tic56), three chloroplast translocon-associated proteins (Ctap3, Ctap4 and Ctap5) and three newly identified small inner-membrane proteins (Simp1-3) have been located in the supercomplex. (nature.com)
  • Type Ix Secretion System : Characterization Of An Effector Protein And An Insight Into The Role Of C-Terminal Domain Dimeration In Outer Membrane Translocation. (bepress.com)
  • Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. (nih.gov)
  • Detection of Known and Novel Small Proteins in Pseudomonas stutzeri Using a Combination of Bottom-Up and Digest-Free Proteomics and Proteogenomics. (mpg.de)
  • Proteomic Characterization of the Pseudomonas sp. (mpg.de)
  • A single molecule of LPS is selected (purified away) from an aggregate by lipopolysaccharide binding protein (LBP)or potentially as a monomer where serum albumin has been found to serve in lieu of LBP. (europeanpharmaceuticalreview.com)
  • Here we report that nonglycosylated full-length and C-terminal truncated amyloid precursor protein (APP) accumulates exclusively in the protein import channels of mitochondria of human AD brains but not in age-matched controls. (jneurosci.org)
  • Using a human cortical neuronal (HCN) culture system, we showed that full-length APP is targeted to both mitochondria and plasma membrane (PM) by virtue of N-terminal chimeric signals. (jneurosci.org)
  • The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix. (nih.gov)
  • This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with activation of the complement system and a range of antimicrobial peptides, such as defensins, cathelicidin and saposins. (bvsalud.org)
  • Predicted to be part of TIM23 mitochondrial import inner membrane translocase complex. (nih.gov)
  • The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. (rndsystems.com)
  • It belongs to a multimeric complex and is associated with other proteins.The pleiotropic actions of TSPO render this protein an interesting target in the nervous system. (uni-regensburg.de)
  • The major constituent of a senile plaque is β-amyloid (Αβ), which is a 40-43 amino acid peptide produced by the action of secretory pathway-associated proteases, namely β and γ secretases, at the C terminus of a type I membrane-spanning glycoprotein termed amyloid precursor protein (APP). (jneurosci.org)
  • Monoclonal antibodies against LipL32, the major outer membrane protein of pathogenic Leptospira: production, characterization, and testing in diagnostic applications. (fiocruz.br)
  • Chen, L. J. & Li, H. M. Stable megadalton TOC-TIC supercomplexes as major mediators of protein import into chloroplasts. (nature.com)
  • Major surface protein 2 (MSP2) is an immunodominant and antigenically variant protein in the outer membrane of the rickettsia Anaplasma marginale. (oregonstate.edu)
  • It also has a role in the resistance against attack by the human complement system a Bacteriophage lambda outer membrane protein, Lom OspA/B are lipoproteins from Borrelia burgdorferi. (wikipedia.org)
  • Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
  • Captured proteins are visualized using chemiluminescent detection reagents (Step 3) . (rndsystems.com)
  • After detection, the array data were quantitated to generate a protein profile (histogram). (rndsystems.com)
  • A six-dimensional alpha proton detection-based APSY experiment for backbone assignment of intrinsically disordered proteins. (mpg.de)
  • NMR-based detection of hydrogen/deuterium exchange in liposome-embedded membrane proteins. (mpg.de)
  • Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins. (mpg.de)
  • The phage protein Lom is expressed during lysogeny, and encode host-cell envelope proteins. (wikipedia.org)
  • A total of 816 +/- 11 individual proteins were identified which included 371 +/- 33, 565 +/- 15 and 262 +/- 5 from the cytosolic, cell envelope and OMP preparations, respectively. (reading.ac.uk)
  • We then transofmed in exprressio constructs for our malE and mutant malE proteins. (igem.org)
  • Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. (bmj.com)
  • Characterization of four outer membrane proteins that play a role in utilization of starch by Bacteroides thetaiotaomicron. (research.com)
  • Biochemical evidence that starch breakdown by Bacteroides thetaiotaomicron involves outer membrane starch-binding sites and periplasmic starch-degrading enzymes. (research.com)
  • OspA from Borrelia burgdorferi is an unusual outer surface protein, it has two globular domains which are connected with a single-layer β-sheet. (wikipedia.org)
  • investigated pathogenesis and immunological response caused by HomB protein in clinical patients and 190 H. pylori strain isolated from patients with peptic ulcer disease (PUD) or gastritis were evaluated for the clinical importance of homB . (nature.com)
  • As most meningococcal strain characterization methods depend on a clinical isolate, this has resulted in a loss of epidemiological information. (ox.ac.uk)
  • The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. (rndsystems.com)
  • These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)
  • Area A will delineate the diagnostic potential of TSPO imaging by means of positron emission tomography (PET) in brain tumors in vivo and will enable the complementary characterization of brain tissue in vitro to delineate the neurobiological characteristics underlying TSPO PET labeling in the human brain. (uni-regensburg.de)
  • Three of the specific aims will focus on characterization of three newly discovered potential colonization factors of EHEC. (usda.gov)
  • The release of mitochondrial-intermembrane-space pro-apoptotic proteins, such as cytochrome c , is a key step in initiating apoptosis. (biologists.com)
  • Antibody responses to outer-membrane proteins have been obtained, predominantly in the IgG3 subclass. (medscape.com)
  • This protein is highly soluble, contains a large number of Lys and Glu residues. (wikipedia.org)
  • DNA homologies, G + C content of the DNA, shape and ultrastructure of the cells, salt requirements, ammonia tolerance, utilization of urea as ammonia source, and whole-cell protein patterns were analysed. (microbiologyresearch.org)
  • Characterization of the epidemiology and clinical features of these and other possible agents of viral hepatitis will await the development of diagnostic assays. (cdc.gov)
  • Borrelia burgdorferi (responsible for Lyme disease) outer surface proteins play a role in persistence within ticks (OspA, OspB, OspD), mammalian host transmission (OspC, BBA64), host cell adhesion (OspF, BBK32, DbpA, DbpB), and in evasion of the host immune system (VlsE). (wikipedia.org)
  • In the first part outer membrane preparation of three relapsing fever Borrelia were investigated. (uni-wuerzburg.de)