Outer bacterial membrane proteins. Medical search. Frequent questions

These transport proteins come in two forms: carrier proteins and channel proteins. (phys.org)
Carrier proteins are involved in using the energy released from ATP being broken down to facilitate active transport and ion exchange. (phys.org)

In this review, we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson's disease, with a special emphasis on functional bacterial amyloid proteins, bacterial biosurfactants, endotoxins and short-chain fatty acids. (techscience.com)
Panning combinatorial phage libraries against recombinant MMADHC allowed mapping of putative sites of interaction on each protein. (mcgill.ca)
Uncharacterized protein of 472 aas and 22 putative β-strands and one α-TMS. (tcdb.org)
Uncharacterized protein of 512 aas and 16 putative β-strains and one α-TMS. (tcdb.org)
Uncharacterized protein of 575 aas with 13 putative β-strains and one N-terminal α-TMS. (tcdb.org)
Further analysis indicated that these two proteins possessed almost identical gene sequences, apart from two insertion/deletion events in predicted external loops present within the putative barrel-like structure of the proteins. (eur.nl)
Another putative protein is also predicted to be an integral membrane protein, while the third is an ABC-transporter protein. (pitt.edu)
We propose that these three putative proteins form a mycobacterial membrane-bound complex involved in protein-dependent transport. (pitt.edu)
In addition, some diatom genera expressed components of a putative Fe-siderophore uptake system, including an Fe-siderophore outer membrane receptor protein and corresponding ABC transporter components. (uri.edu)

Numerous sRNAs have been identified using both computational analysis and laboratory-based techniques such as Northern blotting, microarrays and RNA-Seq in a number of bacterial species including Escherichia coli, the model pathogen Salmonella, the nitrogen-fixing alphaproteobacterium Sinorhizobium meliloti, marine cyanobacteria, Francisella tularensis (the causative agent of tularaemia), Streptococcus pyogenes, the pathogen Staphylococcus aureus, and the plant pathogen Xanthomonas oryzae pathovar oryzae. (wikipedia.org)
Sueki A, Stein F, Savitski MM, Selkrig J * & Typas A* (2020) Systematic Localization of Escherichia coli Membrane Proteins. (ukaachen.de)
For import of iron-siderophore complexes, seven proteins in the cell envelope of Escherichia coli are essential. (mcgill.ca)
2013). Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli . (tcdb.org)
2003). A novel outer membrane protein, Wzi, is involved in surface assembly of the Escherichia coli K30 group 1 capsule. (tcdb.org)
We provide a method to simultaneously screen a library of antibody fragments for binding affinity and cytoplasmic solubility by using the Escherichia coli twin-arginine translocation pathway, which has an inherent quality control mechanism for intracellular protein folding, to display the antibody fragments on the inner membrane. (jove.com)
The method harnesses the intrinsic intracellular folding quality control mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway to display an scFv library on the E. coli inner membrane. (jove.com)

Is the Subject Area "Mitochondrial membrane" applicable to this article? (plos.org)
Many of the pro-inflammatory pathways activated during cell death occur upon mitochondrial outer membrane permeabilization (MOMP), the pivotal commitment point to cell death during mitochondrial apoptosis. (nature.com)
Some of these mitochondrial-activated inflammatory pathways can be traced back to the bacterial ancestry of mitochondria. (nature.com)
The bacterial origin of mitochondria suggests that inflammatory pathways found in cytosol-invading bacteria may be relevant to mitochondrial-driven inflammation after MOMP. (nature.com)
Mitochondrial-derived DAMPs can be bacterial-like. (nature.com)
Is mitochondrial-driven inflammation during cell death driven by bacterial-like DAMPs? (nature.com)
Some of these mitochondrial DAMPs share similarities with pathogen-associated molecular patterns (PAMPs) found in bacteria and may be derived from their bacterial ancestors. (nature.com)
Due to its bacterial ancestry, mitochondrial formylation of methionine is required for translation initiation of mtDNA-derived mRNA [ 7 ] and will therefore be recognised by the same formyl peptide receptors. (nature.com)
The invaginations of the inner mitochondrial membrane & reflects the respiratory activity of the cell. (flashcardmachine.com)
The compartment between the inner and outer mitochondrial membranes. (flashcardmachine.com)
The matrix also contains the mitochondrial genetic machinery-DNA, RNA, and ribosomes-that generates several (but by no means all) mitochondrial proteins. (flashcardmachine.com)
The outer mitochondrial membrane permeable to what? (flashcardmachine.com)
The inner mitochondrial membrane contains what? (flashcardmachine.com)
proteins mediating electron transport and oxidative phosphorylation are bound in the inner mitochondrial membrane, so the respiration rate varies with membrane surface area. (flashcardmachine.com)

Outer membrane porins in Gram-negative bacteria facilitate antibiotic influx. (imperial.ac.uk)
During her postdoctoral studies at the University of Massachusetts Medical School (UMASS), she focused on the structure and function of outer membrane proteins, mainly Occ-family of porins from P. aeruginosa , and their role in bacterial pathogenesis and antibiotic resistance. (nih.gov)

Viruses that infect eukaryotic cells typically bind to a plasma membrane receptor to initiate the reproduction cycle. (virology.ws)
Antibiotics are 'magic bullets' with the aim of targeting parts of the bacterial physiology that are not present in eukaryotic cells. (futurelearn.com)
I attended a Systems biology meeting ESF 2008 where Ron Weiss explained how he used a bacterial to manipulate eukaryotic cells. (igem.org)
CT load was based on the copy number of the major outer-membrane protein (MOMP) gene normalised per copy number of eukaryotic cells (HLA gene). (bmj.com)

MicF in E. coli was found to regulate the expression of a key structural gene that makes up the outer membrane of the E. coli cell. (wikipedia.org)
Structural Insights into the Mechanism of Dynamin Superfamily Proteins. (nih.gov)
Structural proteins are attached to microfilaments in the cytoskeleton which ensures stability of the cell. (phys.org)
The researchers report on studies investigating the structure and function of the Mla protein, in Nature Microbiology , in a paper entitled " Structural Basis for Maintenance of Bacterial Outer Membrane Lipid Asymmetry . (genengnews.com)
In 2015, she joined the Laboratory of Structural Biology Research and the Protein Expression Laboratory at the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS). (nih.gov)
Dr. Eren's research focuses on understanding the structural and mechanistic aspects of Rev-host protein interactions and finding target regions to inhibit Rev activity using antibodies, synthetic peptides, and aptamers. (nih.gov)
How does an amino acid sequence that is unfolded when it leaves the ribosome manage to fold properly into a highly ordered, lightning-fast enzyme or sturdy structural protein? (anl.gov)
Our goal is to use high-resolution structural modeling techniques guided by constraints taken from lower resolution experimental data to generate structural models of important biological protein assemblies for which high resolution structural determination is unfeasible. (lu.se)

The space between the two membranes is referred to as the periplasmic space in gram-negative bacteria and the intermembrane space in mitochondria [ 6 ]. (nature.com)
Using c-Myc epitope tag insertions and cysteine substitution mutagenesis, we discovered that HMW1B contains an N-terminal surface-localized domain, an internal periplasmic domain, and a C-terminal membrane anchor. (duke.edu)
Functional and biochemical analysis of the c-Myc epitope tag insertions and a series of HMW1B deletion constructs demonstrated that the periplasmic domain is required for secretion of HMW1 and that the C-terminal membrane anchor (HMW1B-(234-545)) is capable of oligomerization and pore formation. (duke.edu)
We speculate that all TpsB proteins have a modular structure, with a periplasmic domain that interacts with the cognate TpsA protein and with pore forming activity contained within the C terminus. (duke.edu)
The periplasmic chaperone community ensures the biogenesis of bacterial outer membrane proteins (OMPs) and has just lately been recognized as a promising goal for antibiotics. (eqalix.com)

We use FhuA from E. coli as a model bacterial outer MP. (mcgill.ca)
The accompanying video shows a scale model for interaction of known proteins of E. coli that participate in transport of iron-siderophores across the cell envelope. (mcgill.ca)
2016). Key diffusion mechanisms involved in regulating bidirectional water permeation across E. coli outer membrane lectin. (tcdb.org)
This plasmid is designed to express tagged proteins in E. coli. (sigmaaldrich.com)

Cryo-EM of the dynamin polymer assembled on lipid membrane. (nih.gov)
Proteins in lipid membranes are one of the fundamental building blocks of biological functionality. (phys.org)
Blocking the protein, which is part of the Mla (maintenance of outer membrane lipid asymmetry) system, would effectively disrupt the composition of one of the two layers of the bacterium's outer membrane, which normally acts as a barrier to toxic chemicals, such as antibiotics. (genengnews.com)

These NFPs are a chemoattractant for host phagocytes and can be recognised by formyl peptide receptors on the plasma membrane [ 8 , 9 ]. (nature.com)
The structure and spontaneous curvature of clathrin lattices at the plasma membrane. (nih.gov)

At least eight different chaperones, arranged in an assembly line, transport β-barrel outer membrane proteins (Omps) and fold them into the membrane. (unibas.ch)

Unlike crystals that are grown for soluble proteins (usually to 100 µm in size), the MP crystals of the ExbB-ExbD complex that we reproducibly grow in lipidic cubic phase are "showers", only 5 to 10 µm in all dimensions. (mcgill.ca)
This is thought to make globular proteins stable and soluble in the watery environment of the cell. (anl.gov)
Moreover, each forms of protein are poorly soluble close to their isoelectric level. (eqalix.com)
The Tat pathway ensures that only soluble, well-folded proteins are transported out of the cytoplasm and displayed on the inner membrane, thereby eliminating poorly folded scFvs prior to interrogation for antigen-binding. (jove.com)

The 6S RNA binds to RNA polymerase and regulates transcription, tmRNA has functions in protein synthesis, including the recycling of stalled ribosomes, 4.5S RNA regulates signal recognition particle (SRP), which is required for the secretion of proteins and RNase P is involved in maturing tRNAs. (wikipedia.org)
Scholars@Duke publication: Translocator proteins in the two-partner secretion family have multiple domains. (duke.edu)
The two-partner secretion pathway in Gram-negative bacteria consists of a TpsA exoprotein and a cognate TpsB outer membrane translocator protein. (duke.edu)
Protein secretion systems are complex molecular machineries that translocate proteins through the outer membrane and sometimes through multiple other barriers. (bvsalud.org)
Here, we describe how to identify protein secretion systems in bacterial genomes using the MacSyFinder program. (bvsalud.org)
It can be used with a set of predefined MacSyFinder models, "TXSScan," to identify all major secretion systems of diderm bacteria (i.e., with inner and LPS-containing outer membranes) as well as evolutionarily related cell appendages (pili and flagella). (bvsalud.org)
For this, it identifies and clusters co-localized genes encoding proteins of secretion systems using sequence similarity search with Hidden Markov Model (HMM) protein profiles. (bvsalud.org)
In this chapter, we describe a complete pipeline of analysis, starting from (i) the integration of information from a reference set of experimentally studied systems, (ii) the identification of conserved proteins and the construction of their HMM protein profiles, (iii) the definition and optimization of "macsy-models," and (iv) their use and online distribution as tools to search genomic data for secretion systems of interest. (bvsalud.org)
Previous work in this area has been on modeling the assembly structure of the type III secretion system and prediction of the high-resolution structures of homo-oligomeric proteins using limited experimental data from Nuclear Magnetic Resonance (NMR) in order to speed up the process of structure determination. (lu.se)

NFPs are mainly found in bacteria where formyl modified methionine initiates protein synthesis [ 7 ]. (nature.com)
Quinolones inhibit two enzymes that are required for bacterial DNA synthesis, i.e. (cdc.gov)
The _____ membrane encloses the stroma, a concentrated solution of ______, including those required for ______ synthesis. (flashcardmachine.com)
The stroma also contains the ___,____, and ribosomes involved in the synthesis of several chloroplast proteins. (flashcardmachine.com)
2022 ) Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae . (lu.se)
This review summarizes some immunological factors involved in the development and control of this oral disease, such as: the participation of inflammatory cells in local inflammation, the synthesis of chemotaxis proteins with activation of the complement system and a range of antimicrobial peptides, such as defensins, cathelicidin and saposins. (bvsalud.org)

alters an outer membrane protein which prevents the drug getting into the bacterial cell and accessing its intracellular target. (futurelearn.com)
While the molecular nature of intracellular cobalamin metabolism in mammals remains poorly understood, the proteins MMACHC, MMADHC, LMBD1 and ABCD4 are implicated in its early uptake and processing. (mcgill.ca)
Mechanisms by which E. chaffeensis establishes intracellular infection, and avoids host defenses are not well understood, but involve functionally relevant host-pathogen interactions associated with tandem and ankyrin repeat effector proteins. (frontiersin.org)
Despite its small genome and limited number of effector proteins, Ehrlichia efficiently establishes an intracellular infection and avoids immune defenses in vertebrate and invertebrate hosts through complex molecular and cellular reprogramming strategies. (frontiersin.org)
Commonly used methods for the display and screening of recombinant antibody libraries do not incorporate intracellular protein folding quality control, and, thus, the antigen-binding capability and cytoplasmic folding and solubility of antibodies engineered using these methods often must be engineered separately. (jove.com)

The Scholars of the Podcast reveal ribosomal proteins encoded in viral genomes, and a protein cell receptor for bat influenza viruses. (virology.ws)
Membrane receptor proteins serve as connection between the cell's internal and external environments. (phys.org)

Researchers have developed models of bacterial outer membranes that can help develop better antibiotics to fight antibiotic-resistant bacteria. (phys.org)
Maintaining dosing is important for some antibiotics in minimising the selection of antibiotic resistant mutants in high density bacterial populations. (futurelearn.com)
On the 2016 WAP cruise, a bacterial addback experiment was completed with these previously antibiotic-treated diatoms under Fe-rich (+Fe) and Fe-limited (-Fe) conditions. (uri.edu)

Molecular chaperones play a key role in cellular processes, including protein homeostasis, but also in membrane protein transport and biogenesis. (unibas.ch)
Figure 2: Outer membrane biogenesis by BAM. (unibas.ch)
The outer membrane group 1 antigen capsule biogenesis/assembly protein, Wzi of 477 aas. (tcdb.org)

Furthermore, gram-negative bacteria and mitochondria both have a double phospholipid membrane layer-the inner and outer membrane. (nature.com)

The inner membrane encapsulates the cytosol of gram-negative bacteria and the matrix of mitochondria and is rich in the phospholipid cardiolipin. (nature.com)
Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
Gram-negative bacteria are surrounded by both an inner membrane (IM) and an outer membrane (OM). (genengnews.com)
OM asymmetry, or more precisely the LPS in the outer leaflet, is the basis for the high intrinsic resistance of Gram-negative bacteria toward antibiotics and other toxic compounds," van den Berg explained to GEN . "This is due to the very special properties of LPS that are unique to Gram-negative bacteria. (genengnews.com)
Hijacking pathogenic membrane proteins to engineer cellular entry: A molecular biophysics approach Invasive pathogenic bacteria feature many cellular niches and life cycles, for which they have developed functions that are potentially attractive in biotechnology and therapeutic delivery applications. (umich.edu)
Endotoxin, or lipopolysaccharide (LPS), is a constituent of the outer leaflet (OL) of the outer membrane (OM) of gram-negative bacteria. (europeanpharmaceuticalreview.com)
It is a unique molecule used as a marker of prokaryotic invasion by metazoan immune systems and occurs as a singular constituent among multiple gram-negative bacteria OM constituents, including phospholipids and surface proteins. (europeanpharmaceuticalreview.com)
it is the product of a bacterial manufacturing process that involves nine separate enzymatic events, followed by export to the gram-negative bacteria cell surface. (europeanpharmaceuticalreview.com)
Unique sugars in LPS include the core sugar, KDO (3 deoxy-α-D-manno-octulosonic acid), and unique arrangements of sugars in the O-antigen moiety, which are used to distinguish bacterial strains when characterising various gram-negative bacteria foodborne illness outbreaks (serotyping). (europeanpharmaceuticalreview.com)
Recently, darobactin A, a novel bicyclic heptapeptide that selectively kills Gram-negative bacteria by targeting the outer-membrane protein BamA, was discovered. (sdsc.edu)
The system involves the study of what are termed autotransporter proteins, which pathogenic bacteria secrete as virulence factors for infection. (anl.gov)
The secretory pathway translocates unfolded proteins from the reducing cytoplasm into the endoplasmic reticulum lumen in yeast or into the periplasm in bacteria. (jove.com)

Molecular mechanics underlying flat-to-round membrane budding in live secretory cells. (nih.gov)
This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. (sigmaaldrich.com)
These methods are powerful and effective for identifying antibodies that bind to targets, yet they depend on the secretory pathway to transport proteins that will be displayed 14-16 . (jove.com)

Recently, we have systematically investigated how molecular chaperones interact with the intrinsically disordered protein α-synuclein. (unibas.ch)
The possible roles of molecular hydrogen, a common by-product of bacterial fermentation, are also addressed. (techscience.com)
Conclusions This study provides the first evidence that quantifiable bacterial protein signals are associated with CD, which can have a profound impact on future molecular diagnosis. (bmj.com)
There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)

In this study, we characterized the functional domains of the Haemophilus influenzae HMW1B protein, a TpsB protein that interacts with the H. influenzae HMW1 adhesin. (duke.edu)
The researchers carried out X-ray crystallography to elucidate the 3D structure of MlaA, combined with computation and functional assays to identify important residues in the protein. (genengnews.com)
The enzymatic cascade used to "build" LPS, as encoded in the bacterial genome, is not shown but is extensively detailed by Wang and Quinn and supports that LPS is a specific functional unit. (europeanpharmaceuticalreview.com)
This has important functional implications because it would result in a locally greater pH gradient across cristal membranes than across inner membranes that are not part of cristae, thereby significantly influencing the rate of oxidative phosphorylation (Section 18-3). (flashcardmachine.com)

Bacterial sRNAs affect how genes are expressed within bacterial cells via interaction with mRNA or protein, and thus can affect a variety of bacterial functions like metabolism, virulence, environmental stress response, and structure. (wikipedia.org)
Attachment of bacteriophages to bacterial cells is more diverse. (virology.ws)
Each of our cells is surrounded by a complex membrane that functions as a biological border, letting ions and nutrients such as salt, potassium and sugar in and out. (phys.org)
Cell recognition proteins allow cells to identify each other and interact. (phys.org)
One such function is tissue-specific bacterial engulfment in human cells which do not normally undergo phagocytosis. (umich.edu)
States have been and continue to be prepared from suspensions of inactivated or disrupted Bordetella pertussis whole bacterial cells. (cdc.gov)
They do this by either collapsing proteins in bacterial cells or damaging the outer membrane of viruses and similar pathogens. (purewatergazette.net)
99.9% of the bacterial cells by serum treatment, the colorimetric assay was used to screen 1000 colonies, of which 35 showed enhanced serum resistance. (uni-wuerzburg.de)
They have the ability to modulate protein activity by binding to a target protein inside cells to prevent protein-protein interactions, disrupt protein-nucleic acid interactions, or prevent substrate access to enzymes 1-5 . (jove.com)

Unexpectedly, MlaA is an alpha-helical integral membrane protein (not a humble lipoprotein as predicted), mostly located in the inner leaflet of the OM but partly inserted into the outer leaflet," writes lead co-research and lead author, Javier Abellon-Ruiz, in an accompanying blog . (genengnews.com)
Four closely-spaced open reading frames (ORF) were identified although two of these may cooperate to produce an integral membrane component of the transport system via a programmed translational frameshift. (pitt.edu)

However, previous work by this team of researchers from the University of Notre Dame and The University of Chicago has shown that, contrary to popular belief, intrinsically disordered proteins, which sample a variety of conformational forms the way the autotransporter proteins do, can remain quite extended in water even if they have high ratios of hydrophobic to charged amino acids. (anl.gov)

Scientists at the U.K.'s University of Newcastle suggest that a bacterial membrane protein that acts as a type of membrane vacuum cleaner could represent a promising new target for rendering harmful Gram-negative strains susceptible to existing as well as new antibiotics. (genengnews.com)
Evidence for this reconnaissance emerged in 2009, when University of California, Davis, plant pathologist Pamela Ronald discovered a bacterial protein called Ax21 in some strains of rice. (discovermagazine.com)
Nowadays, the production of full Wolbachia genomes does not require the physical isolation of the bacterial strains from their respective hosts, and the bacterium is often sequenced as a by-catch of host genomic projects. (bvsalud.org)

Many proteins, largely derived from Bacteroides species, were over-represented, while under-represented proteins were mostly from Firmicutes and some Prevotella members. (bmj.com)
The β-lactam family of drugs target the bacterial cell wall and therefore, would not have any effect on Mycoplasma species. (futurelearn.com)
MPORTANCE Regardless of being prevalent in quite a few ecologically and clinically related bacterial species, the organic function of proteins with a website of unknown perform, DUF1127, is unclear. (eqalix.com)
Our group is interested in the research of clinically relevant bacterial species that mainly cause upper and lower airway infections and their interactions with the host. (lu.se)

This finding suggests that spacing of hydrophobic amino acids must be under selective pressure in bacterial proteins to facilitate their virulence functions. (anl.gov)
Most of them are single-domain proteins, and a big fraction qualifies as small proteins with fewer than 50 amino acids. (eqalix.com)
PapB/FocB family proteins are small DNA-binding transcriptional regulators consisting of approximately 100 amino acids that have been reported to regulate the expression of various fimbriae, including P, F1C, and type 1 fimbriae, and adhesins. (bvsalud.org)

Following removal of the outer membrane, the scFvs displayed on the inner membrane are panned against a target antigen immobilized on magnetic beads to isolate scFvs that bind to the target antigen. (jove.com)

The seven proteins fall into two subclasses on the premise of their size, sequence, and reciprocal regulation by the LysR-type transcription issue LsrB. (eqalix.com)

So, if you are a Master's student looking for a challenging but also rewarding research project, who is interested in bacterial genetics, we encourage you to apply to our project. (lu.se)

These proteins are synthesized in the bacterial cytoplasm and cross one membrane into the bacterial periplasm. (anl.gov)

For instance, mtDNA and bacterial DNA are highly similar thereby activating similar cell autonomous immune signalling pathways. (nature.com)
In this review, we discuss how mitochondria can initiate inflammation during cell death highlighting parallels with bacterial activation of inflammation. (nature.com)
A membrane protein is a protein molecule that is attached to, or associated with the membrane of a cell or an organelle. (phys.org)
Membrane enzymes produce a variety of substances essential for cell function. (phys.org)
Also, all applications where a bacterial cell will be used within a eukaryotic organism (medical applications) are far from being ethically approved. (igem.org)
The "molecule" could for instance be a cancer cell or a viral protein. (igem.org)
The Coulton research group studies membrane proteins (MPs) that are required for transport of iron, an essential nutrient, across the bacterial cell envelope. (mcgill.ca)
The mechanism by which the capsule assembles around the bacterial cell is poorly understood. (tcdb.org)
Why don't all the proteins in the cell instead just stick to each other, aggregating into a big mess? (anl.gov)
The proteins then fold under oxidizing conditions and are displayed on the cell surface or packaged into phage particles to screen for binding affinity 17,18 . (jove.com)
This macromolecular assembly comprises more than 20 conserved proteins that form a series of ring-like structures and a needle-shaped protrusion, which allows the transport of proteins into a host cell. (lu.se)

the objective of this thesis was to characterize the protein-protein interactions that coordinate this process. (mcgill.ca)
In this publication, we characterize a novel outer membrane protein of Moraxella catarrhalis which exists in two variant forms associated with particular genetic lineages, and both forms are suggested to contribute to bacterial clearance from the lungs. (eur.nl)
The primary targets of this analysis have been to develop and characterize Maillard conjugates from pea protein (PPI) or caseinate and dextran, and to guage the bodily stability of nanoemulsions made with such emulsifiers at numerous ionic strengths, pH = 4.6, and temperatures throughout storage, in addition to lutein coloration retention over storage. (eqalix.com)

Mutants functionally deficient in vivo show no binding to K30 polymer in vitro.Apparently, Wzi is a novel outer-membrane lectin that assists in the formation of the bacterial capsule via direct interaction with capsular polysaccharides. (tcdb.org)
Neisseria meningitidis employs polysaccharides and outer membrane proteins to cope with human serum complement attack. (uni-wuerzburg.de)

Similar to our observations with HMW1B, examination of a Bordetella pertussis TpsB protein called FhaC revealed that the C terminus of FhaC (FhaC-(232-585)) is capable of pore formation. (duke.edu)
For this work, they turned to the N-terminal passenger domain of the pertactin virulence protein from Bordetella pertussis, a typical autotransporter. (anl.gov)

We systematically recognized and characterised the seven DUF1127 members of the plant pathogen Agrobacterium tumefaciens All of them give rise to genuine proteins and are differentially expressed as proven on the RNA and protein ranges. (eqalix.com)
Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)

Mutations that result in alterations of the outer membrane porin proteins of Gram-negative organisms lead to decreased permeability of the drug through the outer membrane so less drug reaches the target enzyme. (cdc.gov)

It is now known that most bacterial sRNAs are encoded by free-standing genes located in the intergenic regions (IGR) between two known genes. (wikipedia.org)
Protein profiling and transcriptome sequencing (RNA-seq) evaluation of the wild sort and triple mutant revealed a lot of differentially regulated genes in late exponential and stationary development. (eqalix.com)
In the bacterial metatranscriptome, upregulation of Fe-siderophore uptake genes was observed, possibly indicating increased competition for Fe among the stimulated diatom-associated bacterial community. (uri.edu)

Generally, sRNAs can bind to protein targets and modify the function of the bound protein. (wikipedia.org)
Transcription factors are specific proteins that bind to distinct sites on the genome. (biomedcentral.com)
Opacity-associated (Opa) proteins of Neisseria gonorrhoeae and N. meningitides are eight-stranded outer membrane proteins that bind to different host receptors, triggering engulfment of the bacterium. (umich.edu)

The most potent compound, darobactin B, was used for co-crystallization with the target BamA, revealing an identical binding site to darobactin A. Besides its potency, darobactin B did not exhibit cytotoxicity and was slightly more active against Acinetobacter baumanii isolates than darobactin A. Furthermore, we evaluated the plasma protein binding of darobactin A and B, indicating their different pharmacokinetic properties. (sdsc.edu)

During studies to investigate the outer membrane proteins of this bacterium, two novel major proteins, of approximately 19 kDa and 16 kDa (named OMP J1 and OMP J2, respectively), were identified. (eur.nl)

Results The overall mean logarithmic bacterial load was 1.50 MOMP/HLA for women and 1.15 MOMP/HLA for men. (bmj.com)

They correspond to functions allowing opportunistic pathogens to colonise the mucus layers, breach the host barriers and invade the mucosae, which could still be aggravated by decreased host-derived pancreatic zymogen granule membrane protein GP2 in CD patients. (bmj.com)
There is great need for therapeutics against multi-drug resistant, Gram-negative bacterial pathogens. (sdsc.edu)

Depending on the degree of dehydration, dry mucous membranes, hypotension, prolonged capillary refill time, and poor skin turgor may be present. (medscape.com)

Finally, transport proteins play an important role in the maintenance of concentrations of ions. (phys.org)
Dr. Eren received her Ph.D. from Worcester Polytechnic Institute (WPI), where she studied metal transport mechanisms across membranes by P-type ATPases. (nih.gov)
It is freely permeable only to GAS O2, CO2, and H2O and contains, in addition to respiratory chain proteins, numerous transport proteins that control the passage of metabolites such as ATP, ADP, pyruvate, Ca2+, and phosphate. (flashcardmachine.com)
Contain transport Proteins for transportation of metabolites! (flashcardmachine.com)

More than half of all proteins interact with membranes. (phys.org)
Rev has been reported to interact with many host proteins including importin-ß, histone chaperons (Nap1 and B23), and tubulin through its ARM domain. (nih.gov)
Chapter 4 investigated how three SO diatoms ( Fragilariopsis cylindrus , Pseudo-nitzschia arenysensis , and Thalassiosira tumida ) interact with associated bacterial communities under Fe stress. (uri.edu)

The fluoroquinolone susceptibility profile for each clinical isolate is determined by the number and location of mutational changes in specific enzyme target sites, porin proteins, and efflux mechanisms. (cdc.gov)

Tong J, Dolezal P, Selkrig J , Crawford S, Simpson AGB, Noinaj N, Buchanan SK, Gabriel K & Lithgow T (2011) Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana. (ukaachen.de)
Fe addition elicited varying responses by different diatom genera, with more prevalent diatoms experiencing more differential regulation of Fe-responsive pathways and proteins. (uri.edu)

The targets of quinolone activity are the bacterial DNA gyrase and topoisomerase IV, enzymes essential for DNA replication and transcription. (cdc.gov)

The screening used a genetically modified sequence type (ST)-41/44 clonal complex (cc) strain lacking LPS sialylation, polysaccharide capsule, the factor H binding protein (fHbp) and MutS, a protein of the DNA repair mechanism. (uni-wuerzburg.de)

Some attach to bacterial outer membrane proteins, while others attach to appendages such as pili or flagella. (virology.ws)

Here we explore the feasibility of extracting bacterial protein signals relevant to CD, by interrogating myriads of intestinal bacterial proteomes from a small number of patients and healthy controls. (bmj.com)
Results Our 2D-DIGE-based discovery approach revealed an imbalance of intestinal bacterial functions in CD. (bmj.com)
To date, only one bacterial factor has been shown to mediate intestinal adherence by O157:H7, the outer membrane protein intimin which was originally discovered in the Principal Investigator's laboratory. (usda.gov)

Periodontal disease is a chronic bacterial infection that affects the gingiva and bone that supports the teeth. (bvsalud.org)

TonB, partner protein of FhuA, is one of three proteins from the energy-transducing complex TonB-ExbB-ExbD that is embedded in the cytoplasmic membrane. (mcgill.ca)

She currently studies the underlying mechanisms of viral pathogenesis, viral protein-host protein interactions, and the potential use of humanized scFvs and nanobodies for treatment. (nih.gov)

During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. (sigmaaldrich.com)
Because of the porosity of the outer membrane, the intermembrane space is equivalent to the cytosol in its small-molecule composition. (flashcardmachine.com)

Biological membranes consist of a phospholipid bilayer and a variety of proteins that accomplish vital biological functions. (phys.org)
While the IM is formed by a symmetric phospholipid (PL) bilayer, the OM is structured as an asymmetric bilayer, comprising an inner leaflet of PLs and an outer leaflet that is composed of lipopolysaccharides (LPSs). (genengnews.com)

The HIV-1 Rev protein is a key regulatory factor that is essential for both early and late phases of viral replication cycles, and therefore represents an important viral target for drug development. (nih.gov)

Bradley D, Viéitez C, Rajeeve V, Selkrig J , Cutillas PR & Beltrao P (2021) Sequence and Structure-Based Analysis of Specificity Determinants in Eukaryotic Protein Kinases. (ukaachen.de)

After her postdoctoral studies, Dr. Eren joined the National Institute of Allergy and Infectious Diseases (NIAID) at NIH as a research fellow where she studied host immune system manipulation by Rotavirus proteins. (nih.gov)

Anatomy39

Organisms22

Diseases1

Chemicals and Drugs105

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes56

Disciplines and Occupations1

Technology, Industry, Agriculture7

Information Science4

Health Care1

Carrier Proteins2

Putative9

Escherichia7

Mitochondrial14

Porins2

Eukaryotic cells4

Structural8

Periplasmic5

Coli4

Lipid3

Plasma membrane2

Omps1

Soluble4

Secretion9

Synthesis6

Intracellular5

Receptor2

Antibiotic3

Biogenesis3

Inner and outer1

Bacteria12

Secretory3

Molecular4

Functional4

Cells9

Integral2

Intrinsically disordered1

Strains3

Species4

Amino acids3

Antigen1

Seven proteins1

Genetics1

Cytoplasm1

Cell11

Characterize3

Polysaccharides2

Bordetella2

Pathogen2

Permeability1

Genes3

Bind3

BamA1

Bacterium1

MOMP1

Pathogens2

Hypotension1

Transport4

Interact3

Efflux1

Pathways2

Enzymes1

Mechanism1

Appendages1

Intestinal3

Infection1

Tonb1

ScFvs1

Cytosol2

Phospholipid2

Viral1

Determinants1

Immune System1