• Endogenous ouabain and its binding globulin: effects of physical exercise and study on the globulin's tissue distribution," Hypertension Research , vol. 23, supplement, pp. (hindawi.com)
  • J. M. Hamlyn and M. P. Blaustein, "Salt sensitivity, endogenous ouabain and hypertension," Current Opinion in Nephrology and Hypertension , vol. 22, pp. 51-58, 2013. (hindawi.com)
  • If so, physical exercise associated with the increase in epinephrine and norepinephrine should consequently increase endogenous ouabain. (ahajournals.org)
  • Here, we investigated the effect of physical exercise on the endogenous ouabain plasma concentration in several experimental settings as well as the influence of β-blockade and angiotensin-converting enzyme (ACE) inhibition. (ahajournals.org)
  • Several reports have also pointed to the existence of endogenous ouabain- and digoxin-like compounds, but their precise roles in mammalian physiology and various disorders of the circulation are not clear. (jimmunol.org)
  • In an attempt to produce specific Abs for the purification and identification of endogenous ouabain-like compounds, somatic cell fusion was used to produce mAbs specific for ouabain. (jimmunol.org)
  • Since endogenous ouabain (EO) is a neuro-endocrine hormone involved in renal damage, we evaluated the role of EO in renal ischemia-reperfusion injury (IRI). (mdpi.com)
  • Elevated levels of an endogenous ouabain (EO) occur in many patients with essential hypertension (e.g. (ahajournals.org)
  • It may affect the action of endogenous ouabain on heart and circulation, but the physiological function of this protein is unclear. (nii.ac.jp)
  • may affect blood concentrations of endogenous ouabain and which function the cardiotonic binding protein may have in modulating ouabain effects, the effect of physical exercise on endogenous ouabain was studied and the tissue distribution of its binding protein was investigated. (nii.ac.jp)
  • We found that endogenous ouabain changes rapidly in blood upon physical exercise and behaves like expected for a hormone of circulation. (nii.ac.jp)
  • The effects of the opioid-type stressor, immobilization, on severity of ouabain-induced cardiac arrhythmias and the possible involvement of serum catecholamines were investigated in rats. (biomedsearch.com)
  • Thus, we prepared cochlear organotypic cultures from postnatal day-3 rats and treated these cultures with ouabain at 50, 500, and 1000 μ M for different time to elucidate the ototoxic effects of ouabain in vitro and to provide insights that could explain the comparative ototoxic effects of ouabain in vivo . (hindawi.com)
  • Erttmann RR, Damm KH (1974) Probenecid and hepatic transport of 3 H-ouabain in rats. (springer.com)
  • Proof of principle that ouabain rescues development of embryonic kidneys exposed to malnutrition was obtained from studies on pregnant rats given a low-protein diet and treated with ouabain or vehicle throughout pregnancy. (pubmedcentralcanada.ca)
  • To examine whether ouabain-calcium-NF-κB signalling might rescue the development of fetal kidneys exposed to malnutrition, we first used kidney explants from 14-day-old embryonic rats held in culture for 3 days. (pubmedcentralcanada.ca)
  • Normal male SD rats received infusions of vehicle (VEH), rhamnose (RHA), ouabain (OUA), ouabagenin (OGN), dihydroouabain (DHO), isoouabain (ISO), and ouabain in which the lactone ring was opened (ORO)at 30 μg/Kg/24 hr for 4-5 weeks via s.c. osmotic pumps. (ahajournals.org)
  • DR region specific antibody ameliorated but ouabain worsened renal injury in nephrectomized rats through regulating Na,K-ATPase mediated signaling pathways. (bioportfolio.com)
  • Here, we show in a longitudinal magnetic resonance imaging study that Δ 9 -tetrahydrocannabinol (Δ 9 -THC), the main active compound in marijuana, reduces neuronal injury in neonatal rats injected intracerebrally with the Na + /K + -ATPase inhibitor ouabain to elicit excitotoxicity. (jneurosci.org)
  • 1. The influence of single-dose intravenous ouabain on haemodynamics, renal function and vasoactive hormones was investigated in healthy subjects. (clinsci.org)
  • We measured plasma and renal EO variations and markers of glomerular and tubular damage (nephrin, KIM-1, Kidney-Injury-Molecule-1, α1 Na-K ATPase) and the protective effect of the ouabain inhibitor, rostafuroxin. (mdpi.com)
  • The major apoptotic pathways in ouabain-induced spiral ganglion neuron apoptosis culminated in the stimulation of the p53 pathway and triggering of apoptosis by a network of proapoptotic signaling pathways. (hindawi.com)
  • Downstream effects of ouabain signalling include activation of the calcium-dependent transcription factor nuclear factor (NF)-κB and protection from apoptosis 16 . (pubmedcentralcanada.ca)
  • Considering that Ouabain (OUA) may prevent the apoptosis process, in this study we evaluated if OUA is able to avoid the damage caused by Stx2 and SubAB on human glomerular endothelial cells (HGEC) and the human proximal tubule epithelial cell (HK-2) line. (mdpi.com)
  • Due to antagonism between ouabain and K + , the structure represents a low-affinity ouabain-bound state. (pnas.org)
  • Yet, most of the mutagenesis data obtained with the high-affinity state are readily explained by the present crystal structure, indicating that the binding site for ouabain is essentially the same. (pnas.org)
  • Although it is established that it binds with high affinity to the ATPase in E2P-like states [ K d ≈0.7 nM for shark enzyme used here ( 13 )], the ATPase-ouabain complexes formed by the forward (i.e., in the presence of ATP + Na + + Mg 2+ ) and backward (i.e., in the presence of Pi + Mg 2+ ) reactions have different properties ( 14 ). (pnas.org)
  • Yet, ouabain binds to the ATPase even in the E2·2K + state, although at a mM affinity, and stabilizes the occlusion of K + ( 15 ). (pnas.org)
  • As K + ions are found in the transmembrane binding sites, the structure is considered to represent a low-affinity ouabain-bound state ( 14 , 15 ). (pnas.org)
  • These data suggest that, among the groups we studied, black girls with hypertensive parents had erythrocytes with the lowest binding affinity for ouabain. (ahajournals.org)
  • In assays of fluorescence quenching and saturation equilibrium with tritiated ouabain, Ab 1-10 exhibited 200 nM affinity for ouabain. (jimmunol.org)
  • Ouabain, a representative cardenolide, was found to potently diminish TGEV titers and inhibit the TGEV-induced production of IL-6 in a dose dependent manner, with 50% inhibitory concentrations of 37 nM and 23 nM respectively. (bioportfolio.com)
  • Ouabain as a mammalian hormone," Annals of the New York Academy of Sciences , vol. 986, pp. 678-684, 2003. (hindawi.com)
  • The results demonstrate a new mechanism for steroid signaling via plasma membrane receptors and underline a novel role for the steroid hormone, ouabain, as a physiological inducer of [Ca2+](i) oscillations involved in transcriptional regulation in mammalian cells. (diva-portal.org)
  • Ouabain is a highly toxic compound with a LD50 of 5 mg/kg when administered orally to rodents. (wikipedia.org)
  • Identification and characterization of a ouabain-like compound from human plasma," Proceedings of the National Academy of Sciences of the United States of America , vol. 88, pp. 6259-6263, 1991. (hindawi.com)
  • 5. The present study demonstrates that ouabain has significant although minor, acute effects on plasma levels of vasoactive hormones. (clinsci.org)
  • It fails, however, to confirm that ouabain is an acute pressor or natriuretic substance. (clinsci.org)
  • SUMMARY The effects of ouabain, 20 μg/kg, and isoproterenol, 0.03 μg/kg/min, were compared in conscious dogs with acute myocardial ischemia. (ovid.com)
  • Ouabain, that has been isolated from bovine adrenals and hypothalamus, is a new cardiotonic steroid hormone, which is either synthesized in the adrenals or stored there after it has absorbed from the diet. (nii.ac.jp)
  • As expected, serum-deprived kidneys displayed severe retardation of nephron formation and robust increase in apoptotic index (AI), but these effects were abolished if the kidneys were exposed to ouabain in concentrations that had no effect on intracellular sodium. (pubmedcentralcanada.ca)
  • An increase 9 as well as a decrease of plasma concentrations of ouabain-like compounds (OLCs) 10,11 have been reported. (ahajournals.org)
  • 7 Bovine adrenocortical cells in tissue culture release ouabain on exposure to epinephrine, angiotensin II, or corticotropin. (ahajournals.org)
  • Ouabain activates NF κ B through an NMDA signaling pathway in cultured cerebellar cells," Neuropharmacology , vol. 73, pp. 327-336, 2013. (hindawi.com)
  • NEM activates ouabain-insensitive Cl-dependent K efflux in low K sheep and goat red blood cells. (wikipedia.org)
  • It is concluded, first that hepatocellular uptake of ouabain is mediated by a carrier for steroids and second that the pathway of release is distinct from that of uptake. (springer.com)
  • Kupferberg HJ, Schanker LS (1968) Biliary secretion of ouabain- 3 H and its uptake by liver slices in the rat. (springer.com)
  • Cornog, G. L., Gonatas, N. K., Feierman, J. R.: Effects of intracerebral injection of ouabain on the fine structure of rat cerebral cortex. (springer.com)
  • 2. After ouabain injection there was a significant reduction in minimum and average hourly heart rates. (clinsci.org)
  • 4. Ouabain injection was associated with a fall in plasma angiotensin II levels and a rise in plasma adrenaline levels, but plasma noradrenaline levels were unchanged. (clinsci.org)
  • The downstream signals through ouabain-triggered protein phosphorylation events include activation of the mitogen-activated protein kinase (MAPK) signal cascades, mitochondrial reactive oxygen species (ROS) production, as well as activation of phospholipase C (PLC) and inositol triphosphate (IP3) receptor (IP3R) in different intracellular compartments. (wikipedia.org)
  • The predominant fraction of intracellular ouabain beeing unbound. (springer.com)
  • To assess the role of the ouabain-calcium-NF-κB signalling pathway, we deprived the intracellular stores of calcium and blocked the transcriptional capacity of NF-κB. (pubmedcentralcanada.ca)
  • 500 μ M ouabain and 1000 μ M ouabain were destructively traumatic to both spiral ganglion neurons and cochlear hair cells in an apoptotic signal-dependent pathway. (hindawi.com)
  • Our results suggest that the synergism between ouabain and BDNF occurs through the activation of the Src pathway, JNK, PLC, and PKCδ. (springer.com)
  • We propose that if ouabain is present in the human circulation, its concentration is less than 0.005 nmol/L. (ahajournals.org)
  • Post had recently discovered that three sodium ions were pumped out of the cell for every two potassium ions pumped in, and in his research he had made use of a substance called ouabain which had recently been shown to inhibit the pump. (wikipedia.org)
  • At this stage Skou was unaware that ouabain inhibited the pump, but he immediately telephoned to his lab and arranged for the experiment to be done. (wikipedia.org)
  • Furthermore, the presence of a sugar group in ouabain and the absence of one in arenobufagin suggests that it may me more lipophilic that ouabain and as such form a more stable complex with the Na+-K+ pump. (wikipedia.org)
  • Our attempts to produce ouabain-specific mAbs were unsuccessful when ouabain was coupled to exogenous proteins such as bovine γ-globulins, BSA, and human serum albumin. (jimmunol.org)
  • The structure was solved by molecular replacement starting from the ouabain-unbound form ( 16 ) and refined to an R free of 29.3% at 2.8 Å resolution ( Table 1 ). (pnas.org)
  • As a whole, structural changes caused by ouabain binding are rather small, limited to transmembrane helices M1-M4 ( Figs. 1 A and 2 A and B ). If the ouabain-unbound structure is aligned to the bound one with the transmembrane region, the cytoplasmic headpiece appears slightly inclined toward the M5-M1 direction (i.e., around the y-axis), reflecting a small change of the M5 helix. (pnas.org)
  • Ouabain appeared to alleviate the ischemic condition, whereas isoproterenol intensified ischemia. (ovid.com)
  • The results showed that ouabain-induced damage in vitro was dose and time dependent. (hindawi.com)