• The expression of gene transcripts for Runx2, Bmp2, Fgfr1, collagen type 1 (Col1), osteocalcin (Oc), and osteopontin (Op) were evaluated by real-time RT-PCR. (techscience.com)
  • Osteoblast cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and Op. Maternal hyperthyroidism increases the activity of matrix synthesis, alkaline phosphatase activity, and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts, extracted from the calvaria of the offspring, which may be one of the mechanisms of premature fusion of cranial sutures. (techscience.com)
  • For phenotypic characterization, cells had been incubated with Fluorescein isothiocyanate (FITC)-conjugated anti-CD90, PerCP-Cy5.5-conjugated anti-CD105, APC-Cy7-conjugated anti-CD45 (all purchased from BD Pharmingen), and PE-conjugated anti-CD34 (Miltenyi Mouse monoclonal to XRCC5 Biotech) and FITC-conjugated anti-bone sialo-protein (BSP) (Biorbyt), anti-CFS-conjugated anti-osteopontin (OPN) (R&D Systems) for the evaluation of osteogenic differentiation. (oscars2019info.com)
  • Runx2 is responsible for expression of osteogenic marker genes, including osteopontin, osteocalcin and ALP. (adenosine-receptor.com)
  • Alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2) are commonly used to evaluate differentiation and maturation of osteoblasts. (frontiersin.org)
  • Moreover, ES in 3D aNFC led to an increased expression of the osteogenic markers osteopontin and osteocalcin and a rearrangement and alignment of the actin cytoskeleton. (upmbiomedicals.com)
  • Generally, alkaline phosphatase (ALP), COL1A1, BSP, RUNX2, transforming growth factor-beta 1 (TGFB1), osteonectin (ON), and bone morphogenetic protein-2 (BMP2) are known to be early markers of osteoblastic differentiation, whereas OC and osteopontin (OPN) are expressed later in the differentiation process [ 20 ]. (biomedcentral.com)
  • Gene expression of runt-related transcription factor 2 (RUNX-2), alkaline phosphatase (ALP), osteocalcin (OC), osteopontin (OPN), bone sialoprotein (BSP), and osterix (OST) was assessed by means of Real-Time PCR at days 7 and 10. (umich.edu)
  • At this later timepoint , SPP1 (Osteopontin) expression was also decreased, whilst COL1A1 (Type-I-collagen) levels elevated and no signifi-cant alterations were observed for SPARC (Osteonectin) or BGLAP (Osteocalcin) expression (Fig. 3D). (itkinhibitor.com)
  • The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. (vutbr.cz)
  • For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)
  • Cell proliferation was assessed using MTT assay, revealing that exendin-4 increased cell proliferation at effective concentrations between 10 −10 and 10 −5 M. Quantitative PCR analysis showed that exendin-4 increased the mRNA expression of the differentiation markers alkaline phosphatase (ALP), collagen-1 (COL1), osteocalcin (OC), and runt-related transcription factor 2 (RUNX2) under osteogenic conditions. (endocrinology-journals.org)
  • This pro-osteogenic effect was demonstrated by the increase in the alkaline phosphatase (ALP) activity, deposition of mineralized nodules and the gene expression of osteogenic markers such as bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin and bone sialoprotein (BSP). (fapesp.br)
  • DDM liposomes promoted the upregulation of 'osteodentine' markers osteocalcin and RUNX2 in DPSCs after 9 days of treatment, determined by Real Time quantitative PCR. (cardiff.ac.uk)
  • During the fusion course of action a metaplastic shift appeared from the arch centra wherever cells inside the intermediate zone concerning osteoblasts and chon drocytes co transcribed col1a, col2a, runx2, osteocalcin make it clear and osteonectin, as visualized by ISH. (pkc-inhibitors.com)
  • Chondrogenesis ( COL2 , ACAN , COL10 ) and osteogenesis ( OSC , ALP , RUNX2 ) marker expression was studied by quantitative RT-PCR. (bmj.com)
  • Determination of gene expression at 7 days showed that the early osteogenic transcription components RUNX2, MSX2 and DLX5 had been drastically upregulated in MPCs treated with CHIR (Fig. 3C). (itkinhibitor.com)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. (nature.com)
  • results showcase the life of a cross-talk between GR and HDAC2, offering a mechanistic description for the impact from the HDAC inhibitor (specifically VPA) on osteogenic differentiation in MSCs. (oscars2019info.com)
  • Thirteen miRNAs promoted and 17 suppressed odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • hsa-miR-140-5p, hsa-miR-218 and hsa-miR-143 were more frequently reported suppressing the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • KEGG pathway analysis revealed MAPK, PI3K-Akt and FoxO as key signaling pathways involved in the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • Recent studies have suggested that both exposure to electrical fields and cultivation in 3D can positively affect osteogenic potential of MSCs. (upmbiomedicals.com)
  • Mesenchymal stem cells (MSCs) are known for their potential to differentiate towards multiple lineages, including osteogenic, chondrogenic, and myogenic29. (healthyguide.info)
  • The differentiation potential of hNDP-SCs and hBM-MSCs to adipogenic, osteogenic, chondrogenic, myogenic and neurogenic was shown. (hacettepe.edu.tr)
  • Osteogenic effects of TSC-EVs on MSCs were assessed by alkaline phosphatase (ALP) activity, calcium assays, and calvarial bone defect animal models. (biomedcentral.com)
  • Osteocalcin binds to hydroxyapatite just in post-proliferative older osteoblasts that are connected with mineralized osteoid [41,42]. (oscars2019info.com)
  • At fused stage, early markers for osteoblasts and chondrocytes had been upregulated whereas the osteoblast inhibitor and genes concerned in chon drocyte hypertrophy have been downregulated, success also supported by ISH. (pkc-inhibitors.com)
  • Osteogenic differentiation of osteoblasts is of great importance, and the regulatory mechanisms are urgent to be studied. (edu.pl)
  • The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch-Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS). RESULTS: Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. (nyu.edu)
  • In order to synthesize bone ex-vivo, electrospun scaffolds of fibrinogen (Fg), polydioxanone (PDO), and Fg:PDO blends were seeded with early passage ASCs, fibroblasts, or osteosarcoma cells and were maintained for 21 days in osteogenic or regular growth media. (vcu.edu)
  • Using SEM, the appearance of regular, mineralized-appearing structures were found in osteogenic-induced ASC seeded scaffolds beyond 14 days, only in the scaffolds containing Fg. (vcu.edu)
  • Further, at 21 days of culture, Fg scaffolds with ASCs in osteogenic media became hard and brittle. (vcu.edu)
  • To confirm our gene expression results, osteogenic-induced ASCs on Fg scaffolds were also shown to secrete osteocalcin in the extracellular matrix, a key marker in osteoblastogenesis. (vcu.edu)
  • hADSCs seeded on CPO scaffolds adhered, spread, and underwent osteogenic differentiation up to 28 days, as shown by SEM, alkaline phosphatase activity, alizarin red staining, and elevated levels of osteocalcin, a late stage bone matrix protein. (uconn.edu)
  • Ashe, Keshia, "Design and Fabrication of Osteogenic and Angiogenic Composite PLAGA/CPO Sintered Microsphere Scaffolds for Bone Tissue Engineering" (2016). (uconn.edu)
  • On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
  • The leaves contain several methoxy-isoflavones and a novel compound that improves the trabecular microarchitecture of the long bones, increases their biomechanical strength, and decreases clinical bone turnover markers (osteocalcin and CTX1), and expression of skeletal osteoclastogenic genes. (vigyanprasar.gov.in)
  • The medicine also helps in new bone formation via the expression of osteogenic genes. (vigyanprasar.gov.in)
  • The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
  • Alkaline phosphatase (ALP) manifestation serves as an early marker, specific osteogenic genes (e.g. collagen I, osteocalcin) are consequently upregulated, and mineralization of the matrix is considered the final and definitive state of osteogenic differentiation30. (healthyguide.info)
  • Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
  • In this study, we assayed the effect of exogenous mouse recombinant GDF-5 on cell proliferation of dental pulp cells and determined the expression levels of odontoblast differentiation marker genes in the cells of isolated mouse dental pulp tissues in the presence of GDF-5. (ispub.com)
  • Here, we investigated the effect of GDF-5 on the proliferation of dental pulp cells and their differentiation into odontoblasts by assaying the expression levels of odontoblast marker genes. (ispub.com)
  • Analysis of gene expression (qRT-PCR) indicated that electrospun Fg supported osteoblastogenesis through the upregulation of alkaline phosphatase and osteocalcin gene expression. (vcu.edu)
  • The giant-cell tumour stromal cells (GCTSC) constitute the neoplastic cells, which are from an osteoblastic origin and are classified based on expression of osteoblast cell markers such as alkaline phosphatase and osteocalcin. (wikipedia.org)
  • The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
  • Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation. (biomedcentral.com)
  • Traditionally and rapidly isolated ASCs were thoroughly characterized as multipotent, having osteogenic, adipogenic, and chondrogenic differentiation potential, and they exhibited comparable proliferative lifespans. (vcu.edu)
  • The influence of PLGA/AgNpPGA/AscH particles on (i) viability and super-oxide production of human umbilical vein endothelial cells in vitro, (ii) morphology and expression of osteogenic markers in osteoblastic MC3T3-E1 cells in vitro, and (iii) antimicrobial activity against a Gram-positive bacterium, methicillin-resistant Staphylococcus aureus , and a Gram-negative bacterium, Escherichia coli , was investigated. (chapman.edu)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
  • He S , Zhang H , Lu Y , Zhang Z , Zhang X , Zhou N , Hu Z , . Nampt promotes osteogenic differentiation and lipopolysaccharide-induced interleukin-6 secretion in osteoblastic MC3T3-E1 cells. (aging-us.com)
  • This study aimed to explore the effect of Nampt in osteogenic differentiation and inflammatory response of osteoblastic MC3T3-E1 cells. (aging-us.com)
  • The dexamethasone (Dex) was used to induce osteogenic differentiation of MC3T3-E1 cells. (edu.pl)
  • test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro. (nyu.edu)
  • 1,2,4] A recent in vitro study also showed an osteogenic effect of MK-7 administration on human mesenchymal cell differentiation. (nuvisionhealthcenter.com)
  • The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
  • Considering that CsinCPI-2 induced the differentiation of human dental pulp cells into osteogenic phenotype, it is important to know the signaling pathways involved in this differentiation, for a better understanding of the effect of the protein on undifferentiated mesenchymal cells and, consequently, to indicate it or not for reparative therapy in endodontics and other areas. (fapesp.br)
  • and (iii) multiple-fold upregulation of two osteogenic markers: osteocalcin and type I procollagen. (chapman.edu)
  • In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in a perfusion bioreactor. (utwente.nl)
  • More osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture. (utwente.nl)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • Results published by our research group showed that cystatin CsinCPI-2, derived from citrus sinensis (sweet orange), was able to inhibit the gene expression and enzymatic activity of human cysteine cathepsins, showed anti-inflammatory activity and, had pro-osteogenic effect on human dental pulp cells. (fapesp.br)
  • Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
  • Objective: The aim of this study was to evaluate the effect of a novel calcium aluminate cement (CAC+) on gene expression of key osteoblastic markers in primary osteogenic cell cultures. (umich.edu)
  • Both cell lines were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. (hacettepe.edu.tr)
  • This ease of availability and their ability to undergo osteogenic differentiation makes ASCs promising candidates for cell-based therapies for bone fractures. (upmbiomedicals.com)
  • Taken together, our data suggest that a combination of ES with 3D cell culture can increase the osteogenic potential of ASCs. (upmbiomedicals.com)
  • To accelerate their osteogenic differentiation, we overexpressed the osteogenic transcription factor Cbfa1 (Core binding factor alpha-1, Til-1) gene using an adenovirus vector in rat ASCs. (jarde.jp)
  • The results revealed that the osteoblastic markers, such as ALP activity, the osteocalcin expression, and calcium content, in Cbfa1-overexpressing ASCs were markedly greater than those of control ASCs. (jarde.jp)
  • These findings suggested that overex-pression of the Cbfa1 gene induced a remarkable and rapid differentiation of ASCs into osteogenic cells. (jarde.jp)
  • ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications. (vutbr.cz)
  • Bone grafting is a common procedure in orthopaedic surgery and the implantation of autologous bone grafts supplying osteoinductive growth factors, osteogenic cells, and a structural scaffold, has become the gold standard for the surgical treatment of bone defects caused by trauma, tumor, infection or congenital abnormalities. (biomedcentral.com)
  • Furthermore, a relationship was identified by us between GR and HDAC2 inhibition after VPA treatment that affects osteocalcin appearance in DPSCs. (oscars2019info.com)
  • Individual DPSCs had been sorted for Compact disc34 and Compact disc90 positive markers utilizing a Fluorescence Activated Cell Sorting (FACS) Aria III BD (BD Biosciences, Milan, Italy). (oscars2019info.com)
  • Alizarin Red staining and alkaline phosphatase (ALP) staining were used to evaluate the role of RAD51 in osteogenic differentiation of OP model cells. (edu.pl)
  • Expression of osteogenesis markers and Alizarin red staining was comparable in the HLA-B27 + and the HLA-B7 + hPDCs in monolayers. (bmj.com)
  • They are derived from an osteoclast-monocyte lineage determined primarily by expression of CD68, a marker for monocytic precursor cells. (wikipedia.org)
  • The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
  • To study direct osteogenesis in hPDCs, cells were cultured as monolayers and stimulated with osteogenic media. (bmj.com)
  • During bone tissue development, there is certainly little osteocalcin creation, and it generally does not reach maximal amounts until the past due levels of mineralization. (oscars2019info.com)
  • There may be a relationship between vitamin K2-MK7-dependent regulation of endothelial function via the MGP to inhibit osteogenic properties in vascular endothelial cells. (aawconline.org)
  • Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. (nyu.edu)
  • The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
  • Method: Osteogenic cells were enzimatically isolated from newborn rat calvarial bone, plated on Thermanox coverslips at 20,000 cells/well, and cultured for up to 10 days. (umich.edu)
  • Overexpression of Nampt overrode the effect of LPS and rescued LPS-induced inhibition on osteogenic differentiation. (aging-us.com)
  • Thus, electrospun Fg is an excellent material for ASC growth, proliferation, and osteogenic differentiation, providing an ideal system for furthering basic bone model-based research and for advancing regenerative medicine. (vcu.edu)
  • In conclusion, AT-MSC present higher osteogenic potential than BM-MSC when extracted from adult dogs. (scielo.br)
  • Overexpressed RAD51 promoted osteogenic differentiation by activating IGF1R/PI3K/AKT signaling pathway in OP. RAD51 could be a potential therapeutic marker for OP. (edu.pl)
  • The absolute maximal increase in osteocalcin was significantly higher in adolescent and child donors than in adults. (tau.ac.il)
  • CONCLUSION: All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media. (nyu.edu)
  • Conclusion: These results indicate that CAC+ supports the up-regulation of key markers of osteoblastic differentiation in osteogenic cell cultures irrespective of the timing of exposure. (umich.edu)
  • The present study supports the existence of sufficient variation among musk melon genetic resources in India, and their classification based on molecular markers will be helpful to accelerate the breeding programme for specific traits. (cambridge.org)
  • Results There was no difference in chondrogenesis markers or in colorimetric tests between HLA-B27 + and HLA-B7 + micromasses. (bmj.com)
  • It has been shown recently in experimental animals that regeneration of bone marrow after ablation is associated with enhanced osteogenic growth factor activity and a systemic increase in bone formation. (tau.ac.il)
  • To assess the possible occurrence of a similar phenomenon in humans, serum markers of bone formation, osteocalcin and alkaline phosphatase, were measured in marrow donors before the aspiration of large amounts of iliac marrow and 1 day to 5 weeks thereafter. (tau.ac.il)
  • When evaluated together with studies on systemic changes during fracture healing and marrow regeneration, these findings suggest that marrow aspiration in humans evokes a systemic osteogenic response. (tau.ac.il)
  • AT-MSC submitted to osteogenic differentiation showed higher MTT conversion in formazan than BM-MSC, under the same conditions on days 7 and 21. (scielo.br)
  • Odontoblast marker gene levels, on the other hand, were significantly elevated after seven days of culture in the presence of GDF-5. (ispub.com)
  • However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs). (utwente.nl)
  • Thus, the objective of the scientific initiation project is to study the possible role of the BMP2-Smad1/5/8 signaling pathway in differentiating of human pulp cells in osteogenic phenotype induced by CsinCPI-2. (fapesp.br)
  • Four groups were formed: BM-MSC in osteogenic medium (1), BM-MSC in basal medium (2), AT-MSC in osteogenic medium (3) and ATMSC in basal medium (4). (scielo.br)
  • In this study, a total of fifty (50) simple sequence repeat (SSR) markers were used to investigate the genetic diversity and population structure of 46 muskmelon accessions of different horticultural or varietal groups. (cambridge.org)
  • Scholarly interests include investigation of molecular markers of human disease that provide diagnostic function, serve as targets for possible therapeutic manipulation, or provide insight into mechanisms of human disease. (stanford.edu)