Osteogenic bone derived osteocalcin. Medical search. Frequent questions

The aim of this study was to conduct a literature review on the use and clinical viability of human bone morphogenetic protein (rhBMP-2) for grafting bone in Dentistry. (bvsalud.org)
Interestingly Chinese medicine which could promote the secretion of osteogenic factors such as collagen type 1 (COL1) bone morphogenetic protein 2 (BMP-2) osteocalcin (OCN) and osteopontin (OPN) and the gene manifestation of BMP-4 runt-related transcription element 2 (Runx2) OCN and OPN has been increasingly applied in osteogenic induction study [18-20]. (researchassistantresume.com)
The expression of bone-associated genes (runt-related transcription factor 2, type I collagen, bone morphogenetic protein 2, osteonectin and osteocalcin) was significantly increased by a culture time of up to 2 weeks, particularly for the composite scaffold loaded with 10% bioactive glass. (mst.edu)
This pro-osteogenic effect was demonstrated by the increase in the alkaline phosphatase (ALP) activity, deposition of mineralized nodules and the gene expression of osteogenic markers such as bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin and bone sialoprotein (BSP). (fapesp.br)
Growth/differentiation factor-5 (GDF-5) belongs to the bone morphogenetic protein (BMP) family, which is expressed in dental pulp tissues. (ispub.com)
Growth/differentiation factor-5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family, which is a subgroup of the transforming growth factor- (TGF-) superfamily. (ispub.com)
In addition, transforming growth factor and bone morphogenetic protein BMP-2 induce Runx2 /Cbfa1 transcriptional expression via the p38 MAPK pathway. (cusabio.com)
Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. (novusbio.com)
works in concert with bone morphogenetic protein-2 (BMP-2) to potentiate osteogenic differentiation of bone marrow-derived mesenchymal stem/stromal cells (BMSCs). (omicsdi.org)
potentiates bone morphogenetic protein-2-stimulated osteoinduction of genetically engineered bone marrow-derived mesenchymal stem cells in vitro. (omicsdi.org)
can potentiate bone morphogenetic protein-2 (BMP-2)-stimulated osteogenic differentiation and chemotaxis of BMSCs in vitro. (omicsdi.org)
Generally, alkaline phosphatase (ALP), COL1A1, BSP, RUNX2, transforming growth factor-beta 1 (TGFB1), osteonectin (ON), and bone morphogenetic protein-2 (BMP2) are known to be early markers of osteoblastic differentiation, whereas OC and osteopontin (OPN) are expressed later in the differentiation process [ 20 ]. (biomedcentral.com)

Cells used in bone engineering are the osteoblasts, osteoprogenitor cells, periosteal cells, and stem cells. (allenpress.com)
The human bone morphogenet ic protein (rhBMP) developed by genetic engineering, was isolated by Urist, in 1965, and it is considered a substance capable of inducing differentiation of mesenchymal stem cells into osteoblasts, the cells that are responsible for the synthesis of bone matrix. (bvsalud.org)
Among them, Runx2 is a key target gene affecting osteogenic activity, and Cbfa1 regulates the differentiation of MSCs into osteoblasts at the transcriptional level. (cusabio.com)
Moreover, AAV9-NEM localized strongly in vivo to cells lining the calcified trabecular bone and transduced primary murine osteoblasts in culture, while WT AAV9 transduced undifferentiated bone marrow stromal cells as well as osteoblasts. (nature.com)
Immunohistochemical studies of antigen localization on cryostat sections of rat calvaria, long bone, and soft tissues demonstrated that five of these MAbs, UBIM 1,2,3,12, and 17, recognize antigens that are restricted to normal rat osteoblasts and chondrocytes. (docksci.com)
This bone turnover comprises the temporal and spatial coordination of two processes performed in bone remodeling units at discrete sites throughout the skeleton: removal of old and damaged bone by osteoclasts, called resorption, which can last 4-6 weeks, followed by new bone formation by osteoblasts, which produce collagen and mineralized bone matrix in a process lasting 4-5 months [ 3 ]. (biomedcentral.com)
Mesenchymal stem cells (MSCs), which are activated by secreted, active transforming growth factor beta 1 (TGFβ) to migrate to bone-resorptive sites and differentiate into osteoblasts (see below). (biomedcentral.com)
They are derived from terminally differentiated osteoblasts surrounded by unmineralized matrix (osteoid) during bone formation. (biomedcentral.com)
Once the osteoid mineralizes, the osteocytes are trapped there and form an extensive network with each other, with osteoblasts, and with the lining cells on the bone surface (explained below). (biomedcentral.com)
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
Here we show that bone marrow endosteal SSCs are defined by fibroblast growth factor receptor 3 ( Fgfr3 ) and osteoblast-chondrocyte transitional (OCT) identities with some characteristics of bone osteoblasts and chondrocytes. (nature.com)
Therefore, Fgfr3 + endosteal SSCs are abundant in young bone marrow and provide a robust source of osteoblasts, contributing to both normal and aberrant osteogenesis. (nature.com)
Osteoblasts and osteoclasts differ in their function in the maintenance of bones. (pediaa.com)
The osteogenic cells in bones are developed into osteoblasts. (pediaa.com)
The osteoblasts secrete the collagen matrix and the calcium salts of a bone. (pediaa.com)
When osteoblasts are trapped inside the bone by calcification, they are transformed into the more mature type of bone cells called osteocytes. (pediaa.com)
Osteoblasts help in both initial bone formation and later bone remodeling processes. (pediaa.com)
Osteoblasts are developed from osteogenic cells in the periosteum. (pediaa.com)
Many products of osteoblasts such as growth factors, collagen , hormones such as osteocalcin, and enzymes such as collagenase and alkaline phosphatase are involved in the bone formation. (pediaa.com)
These trapped osteoblasts become mature bone cells called osteocytes. (pediaa.com)
Both osteoblasts and osteoclasts are found on the surface of bones. (pediaa.com)
The TPF also promoted osteoblasts differentiation and bone formation demonstrated by increasing bone formation markers in cultured mouse primary osteoblasts. (biomedcentral.com)
Therefore, a sustainable drug is desirable to identify better and safe anabolic agents with low toxicity that act by either increasing the osteoblasts proliferation or inducing osteoblasts differentiation to enhance bone formation [ 8 ]. (biomedcentral.com)
Previous studies showed that different plant-derived flavonoid compounds could stimulate osteoblasts function, and inhibit osteoclasts functions either alone or in combination. (biomedcentral.com)
Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)

Osteoprogenitor MSCs not only differentiate into bone, but they also exert modulatory effects on immune cells via a variety of mechanisms. (hindawi.com)
Insight into this paradigm can provide valuable clues in identifying cellular and noncellular targets that can potentially be modulated to enhance both natural bone healing and bone repair augmented by the exogenous addition of MSCs. (hindawi.com)
Coupled with reports that allogeneic MSCs have immunoprivileged status and immunomodulatory properties, there has been considerable interest in exploring the use of these cells as a therapeutic option for bone repair. (hindawi.com)
MSCs were initially isolated from bone marrow but are now known to exist in a wide range of tissues in the human adult, including brain, thymus, lung, liver, spleen, kidney, and dental pulp [ 11 , 12 ]. (hindawi.com)
MSCs have also been derived from embryonic tissues, such as Wharton's jelly and umbilical cord blood [ 13 , 14 ]. (hindawi.com)
Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
By performing local transplants, other non-periodontal-derived MSCs such as dental pulp stem cells (DPSCs), bone marrow stem cells (BMSCs), and adipose tissue-derived stem cells (ADSCs) also aid in the regeneration of periodontal tissue [ 12 ]. (biomedcentral.com)
Adipose-derived stem cells (ASCs) are a readily available population of mesenchymal stem/stromal cells (MSCs) that can be isolated within minimally invasive surgery. (upmbiomedicals.com)
Recent studies have suggested that both exposure to electrical fields and cultivation in 3D can positively affect osteogenic potential of MSCs. (upmbiomedicals.com)
p38 pathway plays a very important role in the osteogenic differentiation of mesenchymal stem cells (MSCs). (cusabio.com)
A comparison was done between the properties of NDP-SCs and the properties of mesenchymal stem cells (MSCs) from bone marrow (BM) of the human. (hacettepe.edu.tr)
The differentiation potential of hNDP-SCs and hBM-MSCs to adipogenic, osteogenic, chondrogenic, myogenic and neurogenic was shown. (hacettepe.edu.tr)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
We reconstituted type I collagen nanofibers prepared by electrospin technology and examined the morphology, growth, adhesion, cell motility, and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) on three nano-sized diameters (50-200, 200-500, and 500-1,000 nm). (duke.edu)
Therefore, type I collagen nanofibers support the growth of MSCs without compromising their osteogenic differentiation capability and can be used as a scaffold for bone tissue engineering to facilitate intramembranous bone formation. (duke.edu)
Mesenchymal stem cells (MSCs) can easily be isolated and expanded from bone marrow (BM) aspirates. (biomedcentral.com)
For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)

The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
Both cell lines were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. (hacettepe.edu.tr)
Traditionally and rapidly isolated ASCs were thoroughly characterized as multipotent, having osteogenic, adipogenic, and chondrogenic differentiation potential, and they exhibited comparable proliferative lifespans. (vcu.edu)
In recent decades, the osteogenic and adipogenic properties of BMSCs have been found to maintain a dynamic balance ( 5 - 8 ). (researchpad.co)
In addition, whether M2 macrophages can regulate the balance of osteogenic and adipogenic differentiation of BMSCs needs to be further explored. (researchpad.co)

8 It stimulates DNA synthesis of rat calvarial cells and increases the production of osteopontin but decreases bone sialoprotein, osteocalcin, alkaline phosphatase activity, and mineralization in bone cells. (allenpress.com)
Moreover, ES in 3D aNFC led to an increased expression of the osteogenic markers osteopontin and osteocalcin and a rearrangement and alignment of the actin cytoskeleton. (upmbiomedicals.com)

On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
Alkaline phosphatase activity demonstrated no differences after 12 days of osteogenic differentiation, and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed comparable osteogenic gene expression of osteocalcin, osteonectin, and ostepontin between cells differentiated on polystyrene and nanofiber surfaces. (duke.edu)

The overexpression of Rev-erbα in osteoblast precursors attenuated the expression of osteoblast marker genes including Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteocalcin (OC). (molcells.org)

These include molecular and cellular materials that tissue engineer the bone. (allenpress.com)
2 , 3 Bone engineering involves the delivery of cells or biologic molecules such as growth factors to a defect site for tissue regeneration. (allenpress.com)
In vitro and in vivo results implied that Cbfa1-overexpressing ASCs may be an alternative cell source for BMSCs for bone tissue engineering. (jarde.jp)
In Dentistry autogenous bone is the most useful tissue used in pre-prosthetic surgery and rehabilitation treatment of bone defects, thereby contributing to the function and aesthetics 8 . (bvsalud.org)
The success of rehabilitation with dental implants is directly related to the amount of bone tissue of the patient. (bvsalud.org)
Bone tissue marrow-derived mesenchymal stem cells (BMSCs) are widely used in regenerative medicine in light of their ability to differentiate along the chondrogenic and osteogenic lineages. (researchassistantresume.com)
The regeneration of bone tissue defects specifically includes a series of complicated procedures that are controlled by a number of cytokines and natural signals. (researchassistantresume.com)
since bone tissue regeneration cannot effectively occur with out a bloodstream supply achieving the bone tissue defect angiogenesis CEP-28122 is certainly important for bone tissue regeneration in vivo [6-10]. (researchassistantresume.com)
It really is reported that angiogenesis may be the foundational part of bone tissue regeneration particularly in calvarial and limb bone tissue defects [11-13]. (researchassistantresume.com)
It really is proven that angiogenesis takes place before osteogenesis in the recovery of bone tissue defects. (researchassistantresume.com)
A prior study within a rabbit calvarial defect model additional revealed that there is seductive spatial and temporal relationship between newly produced blood vessels and further skeletal bone tissue development [12]. (researchassistantresume.com)
Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. (utwente.nl)
The features of BFPSCs were compared with bone marrow-derived stem cells (BMSCs) as a well known cell source for bone tissue engineering. (utwente.nl)
The aim of this study was to compare the osteogenic potential of mesenchymal stem cells obtained from bone marrow (BM-MSC) with those extracted from adipose tissue (AT-MSC) of adult dogs. (scielo.br)
These cells, which can be derived from liposuction fat and lipoaspirate saline, are sources for patient-derivable extracellular matrix (ECM), fibrinogen (Fg) and adipose tissue extracellular matrix, and may prove useful for synthesizing new bone tissue analogues in vitro. (vcu.edu)
These ASCs also shared an indistinguishable immunophenotype when compared to bone marrow-derived mesenchymal stem cells, suggesting that these cells are an excellent source for bone following tissue engineering experimentation. (vcu.edu)
We demonstrated that AAV9 capsid modified with N -ethyl Maleimide (NEM) increased its tropism more towards murine bone marrow (osteoblast lineage ) while decreased transduction of liver tissue compared to the unmodified capsid. (nature.com)
MAbs UBIM 9 and 21 bind to marrow cells in long bone sections, to the 7-day-old nonadherent cell population from cultured marrow, and to lymphoid tissue in sections of spleen. (docksci.com)
In humans, bone is a highly active tissue which undergoes continuous self-regeneration throughout adulthood to maintain structural integrity in a process called bone remodeling. (biomedcentral.com)
Bone is a very dynamic and heterogeneous tissue formed by two components which are in close relationship with each other during the bone remodeling process: extracellular matrix (ECM) and bone cells. (biomedcentral.com)
The periosteum is the tissue that covers the outer surface of the bone. (pediaa.com)
Since bone is a dynamic tissue, the continuous formation and breakdown occur in it. (pediaa.com)
Sarcomas are classified according to the histologic tissue from which they are derived, and more than 30 histologic subtypes have been described. (medscape.com)
Because of their capacity for ex vivo proliferation and differentiation they provide a good source of osteoprogenitor cells within custom-shaped scaffolds for implantable autologous bone tissue thus allowing the generation of a large transplantable cell population from a small biopsy [ 5 - 11 ]. (biomedcentral.com)
We show that each of these topographic scale ranges represents a scale range found in natural bone tissue. (toysis.pics)
Therefore, in natural bone remodeling, the bonding phenomenon is limited to submicron interlocking [3], however, the long-term physical stability of the interface between new and old bone, which is crucial for the stability of skeletal tissue, depends on the higher-order morphology properties. (toysis.pics)
Beginning at 5 weeks, the mesenchymal tissue organizes to form cartilaginous plates and later, bone that comprises the skull. (medscape.com)

Therefore the most ideal induction method would simultaneously promote the osteogenic differentiation of BMSCs and enhance the manifestation of angiogenic factors. (researchassistantresume.com)
As early as 2002, Champaign found that macrophages could promote the osteogenic differentiation of BMSCs ( 11 ). (researchpad.co)

While some signals are mitogenic and proosteogenic, others function to inhibit osteogenesis and increase bone resorption, and it appears that a well-controlled, delicate balance of inflammatory factors is necessary for proper fracture repair [ 3 - 6 ]. (hindawi.com)
after that both angiogenesis and osteogenesis participate in the bone regeneration and promote the effects of each additional [13]. (researchassistantresume.com)
Bone regeneration is a complex process that requires the coordination of osteogenesis and osteoclastogenesis. (researchpad.co)
The balance between osteogenesis and adipogenesis of bone marrow mesenchymal stem cells (BMSCs) plays a major role in the process of bone formation. (researchpad.co)
In this experiment, we found that exosomes derived from M2 macrophages (M2D-Exos) could inhibit adipogenesis and promote osteogenesis of BMSCs. (researchpad.co)
Taken together, the results of our study indicate that exosomes isolated from M2 macrophages could facilitate osteogenesis and reduce adipogenesis through the miR-690/IRS-1/TAZ axis and might be a therapeutic tool for bone loss diseases. (researchpad.co)
Thus, regulating the balance between osteogenesis and adipogenesis may be a potential treatment strategy for bone loss diseases. (researchpad.co)
Osteal macrophages are located on the bone surface and are tightly related to the osteogenesis of BMSCs ( 10 ). (researchpad.co)
At the cellular level, the mesenchymal stem cell pool in the bone marrow niche shows a biased differentiation into adipogenesis at the cost of osteogenesis. (biomedcentral.com)
In this study, we report a novel population of SSCs with osteoblast-chondrocyte transitional (OCT) identities in the bone marrow endosteal space, which highly expresses Fgfr3 and contributes to both normal and aberrant osteogenesis. (nature.com)
Overall, our findings indicate that Fgfr3 + endosteal stem/stromal cells with OCT identities dictate active and aggressive osteogenesis, identifying these cells as an important regulator of long-term bone homeostasis. (nature.com)
However, contact osteogenesis can result in bone attachment to the implant surface if the implant has a surface topography with submicron physical undercuts [3]. (toysis.pics)

The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)

This study investigated the effect of composite scaffolds composed of poly (octanediol citrate) (POC) and a bioactive glass (composition, 48%SiO2-12%CaO-32%ZnO-8%Ga2O3) on the growth and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). (mst.edu)
All the scaffolds, regardless of the amount of bioglass incorporation, were able to support the growth of hBMSCs and guide their osteogenic differentiation without osteogenic media stimulation. (mst.edu)
These results suggest that composite scaffolds of POC and a bioactive glass can provide favourable conditions for osteogenic differentiation of hBMSCs and can potentially be used to induce bone healing and regeneration. (mst.edu)
In order to synthesize bone ex-vivo, electrospun scaffolds of fibrinogen (Fg), polydioxanone (PDO), and Fg:PDO blends were seeded with early passage ASCs, fibroblasts, or osteosarcoma cells and were maintained for 21 days in osteogenic or regular growth media. (vcu.edu)
Using SEM, the appearance of regular, mineralized-appearing structures were found in osteogenic-induced ASC seeded scaffolds beyond 14 days, only in the scaffolds containing Fg. (vcu.edu)
Further, at 21 days of culture, Fg scaffolds with ASCs in osteogenic media became hard and brittle. (vcu.edu)
Pronounced mineralization was found throughout bone-induced ASC scaffolds, while control scaffolds (BJ foreskin fibroblasts) showed no mineral deposition (although they did demonstrate excellent cellularity). (vcu.edu)
To confirm our gene expression results, osteogenic-induced ASCs on Fg scaffolds were also shown to secrete osteocalcin in the extracellular matrix, a key marker in osteoblastogenesis. (vcu.edu)

Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation. (biomedcentral.com)

After 16 weeks, animals were sacrificed and defect areas evaluated for bone regeneration. (allenpress.com)
Transplanting the VSM/cells constructs to CSDs revealed significant reduction of bone regeneration upon pretreatment of the cells with PDGF-BB. (allenpress.com)
However, short-term application of PDGF-BB to CSD stimulated bone regeneration. (allenpress.com)
However, adding PDGF-BB at the time of surgery had stimulated bone regeneration. (allenpress.com)
Various materials have been used to promote bone regeneration around dental implants, including autografts, allografts, xenografts, and alloplasts. (allenpress.com)
Of these materials, autogenous bone grafts remain the gold standard for bone regeneration. (allenpress.com)
9 , 10 Nevertheless, the role of PDGF in bone regeneration is controversial. (allenpress.com)
The pretreatment of osteoblastic cells with PDGF-BB before transplantation into osseous defects is investigated in the present study to further investigate the role of this growth factor in bone regeneration. (allenpress.com)
In Dentistry, many studies have aimed to improve bone regeneration through the use of BMPs for bone replacement. (bvsalud.org)
Several studies demonstrate the biological advantages of rhBMP-2 on bone regeneration of the jaws. (bvsalud.org)
Methods: Demonstration of this system included (1) development of a piezoelectric PLLA mesh, (2) verification of its piezoelectric efficacy and degradation, (3) manufacturing of a PLLA scaffold, (4) in vitro testing of the system's ability to enhance bone regeneration compared to a control, and (5) using assessments of cell proliferation and differentiation through protein, mineral, and gene assays. (uconn.edu)
Abstract Osteogenic differentiation of various adult stem cell populations such as neural crest-derived stem cells is of great interest in the context of bone regeneration. (uni-bielefeld.de)
It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). (novusbio.com)

The results showed that quercetin significantly enhanced the cell proliferation osteogenic differentiation and angiogenic factor secretion of rBMSCs in a dose-dependent manner with a concentration of 2 μM achieving the best stimulatory effect. (researchassistantresume.com)
These data indicated that quercetin could promote the proliferation osteogenic differentiation and angiogenic factor secretion of rBMSCs in vitro partially through the ERK CEP-28122 and p38 signaling pathways. (researchassistantresume.com)
Thus, electrospun Fg is an excellent material for ASC growth, proliferation, and osteogenic differentiation, providing an ideal system for furthering basic bone model-based research and for advancing regenerative medicine. (vcu.edu)
A recent study suggested that human bone marrow mesenchymal stem cells showed stronger proliferation and osteogenic differentiation when cocultured with inactive human monocytes ( 12 ). (researchpad.co)
BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). (novusbio.com)
Increasing evidence suggests that SDF-1 is involved in bone formation through regulation of recruitment, engraftment, proliferation, and differentiation of stem/progenitor cells. (omicsdi.org)
This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)

In vitro testing showed that relative to a control, in cells subjected to the experimental conditions alkaline phosphatase production increased 5-fold, mineral production increased 18-fold, osteocalcin gene 40-fold, and osterix gene 100-fold. (uconn.edu)
Moreover, we demonstrate an increase of osteogenic transcripts including Osterix, Osteocalcin and up-regulation of Integrin β1 and α2 in the 30 nm pore approach only. (uni-bielefeld.de)

A specific population of adipose-derived stem cells (ASCs) is expected to be an alternate source of bone marrow-derived mesenchymal stem cells (BMSCs). (jarde.jp)
Results: Ultimately a 3000rpm electrospun PLLA nanofiber film that could output 40mV when stimulated with 40kHz 0.4W/cm2 ultrasound was assembled into a bone scaffold and seeded with adipose-derived stem cells (ADSCs). (uconn.edu)
Here, we characterize patient-derivable mesenchymal stem cell types, termed adipose-derived stem cells (ASCs). (vcu.edu)
To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
Mei H, González S, Nakatsu MN, Baclagon ER, Chen FV, Deng SX (2017) Human adipose-derived stem cells support the growth of limbal stem/progenitor cells. (drcremers.com)

Research given to BMPs the capacity of fracture repair, osteogenic, osteoinductive, and osteoconductive potential of the graft 22 . (bvsalud.org)
The autogenous graft type, considered the gold standard, is best used for the rehabilitation of the jaws, because of their osteogenic, osteoconductive, and osteoinductive properties. (bvsalud.org)
Along with other BMPs, including BMP-2, -4 and -7, which are well known as potent osteoinductive growth factors, GDF-5 plays important roles in the development of bones, cartilage and tendons, as evidenced by the tendency for the gene and protein expression levels of GDF-5 to increase over time in these tissues. (ispub.com)
To elucidate the osteoinductive potential of porous substrates with different pore diameters (30 nm, 100 nm), human neural crest-derived stem cells isolated from the inferior nasal turbinate were cultivated on the surface of nanoporous titanium covered membranes without additional chemical or biological osteoinductive cues. (uni-bielefeld.de)
Bone grafting is a common procedure in orthopaedic surgery and the implantation of autologous bone grafts supplying osteoinductive growth factors, osteogenic cells, and a structural scaffold, has become the gold standard for the surgical treatment of bone defects caused by trauma, tumor, infection or congenital abnormalities. (biomedcentral.com)
Among them, bone morphogenic proteins (BMPs) are thought to be the most potent osteoinductive factors and play a key role in the process of bone formation during DO. (hindawi.com)

188 PTH and 1,25-&hydroxyvitamin D3 11,25-(OH)2D,), synthesize and secrete matrix proteins, such as collagen type 1 and osteocalcin, and are osteogenic when implanted in v~vo. (docksci.com)
3%). The organic phase is mainly formed by collagen type I fibers, which provide elasticity and flexibility to bone, but also non-collagenous proteins as well, such as cytokines, growth factors, and proteoglycans. (biomedcentral.com)

Cultivation of human neural crest-derived stem cells on 30 nm pores resulted in osteogenic differentiation as demonstrated by alkaline phosphatase activity after seven days as well as by calcium deposition after 3 weeks of cultivation. (uni-bielefeld.de)

Taken together, our results demonstrate a molecular mechanism of Rev-erbs in the bone remodeling, and provide a molecular basis for a potential therapeutic target for treatment of bone disease characterized by excessive bone resorption. (molcells.org)
The main difference between osteoblast and osteoclast is that osteoblast is involved in the formation and mineralization of bones whereas osteoclast is involved in the breakdown and resorption of bones . (pediaa.com)
An osteoclast refers to a type of bone cell responsible for the bone resorption. (pediaa.com)
Microvilli are extended from osteoclasts to the bone surface, forming a brush-like structure at the active sites of bone resorption. (pediaa.com)
An osteoclast in an area of bone resorption is shown in figure 2 . (pediaa.com)
An osteoclast is a type of bone cell that is responsible for the bone resorption. (pediaa.com)
Recently, we found that the T. procumbens flavonoids (TPF) significantly suppressed the RANKL-induced osteoclasts differentiation and bone resorption. (biomedcentral.com)
Recently, we found the inhibitory effects of the TPF on osteoclast differentiation bone resorption. (biomedcentral.com)
Although those bone substitutes have proven utility, they have often resulted in complications such as stress shielding-induced resorption of the surrounding bone and fatigue failure of the implant. (biomedcentral.com)
This sequence mimics the events that occur at sites of natural bone remodeling where the surface left by resorption of active osteoclasts presents a nanotopographically complex surface with undercuts [3], [4]. (toysis.pics)
Growth at these ossification centers proceeds in a radial fashion with new bone formation (osteoblastic) at the edges and osteoclastic resorption toward the center. (medscape.com)

Considering that CsinCPI-2 induced the differentiation of human dental pulp cells into osteogenic phenotype, it is important to know the signaling pathways involved in this differentiation, for a better understanding of the effect of the protein on undifferentiated mesenchymal cells and, consequently, to indicate it or not for reparative therapy in endodontics and other areas. (fapesp.br)
Inhibition of the p38 MARK pathway down-regulates the activity of protein kinase C ( PKC ), which plays an important role in osteogenic differentiation of cells. (cusabio.com)
Through carboxylation, vitamin K activates osteocalcin, the protein needed to bind calcium to the mineral matrix in bone. (nuvisionhealthcenter.com)

However, limited studies have been conducted on the osteogenic capability of stem cells derived from BFP (BFPSCs). (utwente.nl)
Osteogenic Differentiation of Mesenchymal Stem Cells on a Poly (Octane" by Ehsan Zeimaran, Saktiswaren Mohan et al. (mst.edu)
E. Zeimaran and S. Mohan and S. Pourshahrestani and B. Pingguan-Murphy and N. A. Kadri and M. R. Murali and H. R. Raghavendran and K. Hasikin and T. Kamarul and M. R. Towler, "Osteogenic Differentiation of Mesenchymal Stem Cells on a Poly (Octanediol Citrate)/bioglass Composite Scaffold in Vitro," Materials and Design , vol. 109, pp. 434 - 442, Elsevier, Nov 2016. (mst.edu)
For example, humans are derived from fertilized eggs, and this process involves the differentiation of embryonic stem cells. (cusabio.com)
Dental pulp stem cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar teeth. (hacettepe.edu.tr)
Bone marrow mesenchymal stem cells (BMSCs) are stromal stem cells that are derived from bone marrow and possess multiplex differentiation potential ( 3 ). (researchpad.co)
The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
These Fgfr3 + stem/stromal cells with OCT identities are abundant in the young bone marrow and depleted in the old bone marrow, denoting their transitional nature. (nature.com)
Mesenchymal stem cell expression of stromal cell-derived factor-1? (omicsdi.org)
Numerous studies have revealed that bone marrow-derived mesenchymal stem/stromal cells (BMSCs) hold great potential for regenerative therapies. (omicsdi.org)
Skeletal injuries are among the most prevalent clinical problems and bone marrow-derived mesenchymal stem/stromal cells (BMSCs) have successfully been used for the treatment thereof. (omicsdi.org)
The Tridax procumbens extracts (TPE) are known for their ethno-medicinal properties to increase osteogenic functioning in mesenchymal stem cells. (biomedcentral.com)
These adult stem cells, such as from fat (adipose derived) or bone marrow, have the ability to transform into different cells. (drcremers.com)
The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)

The giant-cell tumour stromal cells (GCTSC) constitute the neoplastic cells, which are from an osteoblastic origin and are classified based on expression of osteoblast cell markers such as alkaline phosphatase and osteocalcin. (wikipedia.org)
JNK can change the mRNA level of osteocalcin , so the activation of JNK can induce osteoblast differentiation. (cusabio.com)
The antigens appear to be localized to the cell surface of the osteoblast, with no apparent staining of bone matrix in either undecalcified or decalcified sections. (docksci.com)
Studies of the interactions of this panel of MAbs with osteogenic tissues and cell lines may have an important impact on the understanding of osteoblast physiology. (docksci.com)
INTRODUCTION HE OSTEOBLAST (OB) is a key cell in bone formation, synthesizing and secreting the organic matrix of bone, and is involved in the regulation of matrix mineralization. (docksci.com)
Osteoblast and osteoclast are two types of cells found in the bone. (pediaa.com)
An osteoblast is a type of bone-forming cell, which is involved in the formation and mineralization of bones. (pediaa.com)
Both osteoblast and osteoclast are involved in the repair and breakdown of bones. (pediaa.com)
An osteoblast is a bone-forming cell that is derived from the mesenchymal osteoprogenitor cells and is involved in the bone remodeling processes. (pediaa.com)
Bone formation-related indices like osteoblast number, osteoblast surface, bone volume, mineralizing surface, mineral apposition rate and bone formation rate were significantly increased in the TPF-treated mice compared to the control mice. (biomedcentral.com)

In this study, the BFPSCs were characterized for their osteogenic differentiation potential especially in contact with a synthetic scaffold in a perfusion bioreactor. (utwente.nl)

Vitamin K is believed to be necessary for bone mineralization. (nuvisionhealthcenter.com)

The results revealed that the osteoblastic markers, such as ALP activity, the osteocalcin expression, and calcium content, in Cbfa1-overexpressing ASCs were markedly greater than those of control ASCs. (jarde.jp)
Serum bone remodelling markers [C-terminal crosslinked telopeptide of type-1 collagen (CTX), procollagen type-1 N-terminal propeptide (P1NP), bone alkaline phosphatase (BAP) and sclerostin] were measured at fasting. (frontiersin.org)
Overall, HR-pQCT, DXA bone parameters and bone turnover markers remained unaffected. (frontiersin.org)

To accelerate their osteogenic differentiation, we overexpressed the osteogenic transcription factor Cbfa1 (Core binding factor alpha-1, Til-1) gene using an adenovirus vector in rat ASCs. (jarde.jp)
These findings suggested that overex-pression of the Cbfa1 gene induced a remarkable and rapid differentiation of ASCs into osteogenic cells. (jarde.jp)
This ease of availability and their ability to undergo osteogenic differentiation makes ASCs promising candidates for cell-based therapies for bone fractures. (upmbiomedicals.com)
Taken together, our data suggest that a combination of ES with 3D cell culture can increase the osteogenic potential of ASCs. (upmbiomedicals.com)

Results published by our research group showed that cystatin CsinCPI-2, derived from citrus sinensis (sweet orange), was able to inhibit the gene expression and enzymatic activity of human cysteine cathepsins, showed anti-inflammatory activity and, had pro-osteogenic effect on human dental pulp cells. (fapesp.br)
The aim of this study was to isolate and extensively characterise SCs derived from human natal dental pulp (hNDP). (hacettepe.edu.tr)

Analysis of gene expression (qRT-PCR) indicated that electrospun Fg supported osteoblastogenesis through the upregulation of alkaline phosphatase and osteocalcin gene expression. (vcu.edu)

However, the availability of viable, autologous bone grafts is limited making alternative cell-based strategies a promising therapeutic alternative. (upmbiomedicals.com)

However, this relationship may not be valid for individual alcohol consumers, as small quantities of alcohol can have detrimental skeletal effects and not all studies report clinically relevant bone loss with long-duration alcohol abuse. (springer.com)
We propose that the interplay between intrinsic factors (e.g., age, sex, skeletal site) and extrinsic factors (e.g., age of onset of drinking, duration of drinking, comorbidities) influence the precise impact of alcohol consumption on bone health. (springer.com)
Skeletal turnover, bone mineral density, and fractures in male chronic abusers of alcohol. (springer.com)

1,2,4] A recent in vitro study also showed an osteogenic effect of MK-7 administration on human mesenchymal cell differentiation. (nuvisionhealthcenter.com)

After the scientific evidence of osseointegration in the ending of the 1970s, which made viable Implantology, increased the interest of researchers in search by new natural or synthetic substances that could replace tissues bone lost 9 . (bvsalud.org)
Biomaterials can be defined as "any material, natural or synthetic, that acts in tissues/organs in order to replace the bone defect lost and s function" 13 . (bvsalud.org)
Approximately 80% of sarcomas originate from soft tissues, while 20% arise from bone. (medscape.com)
Sarcomas are commonly classified according to their site of origin: soft tissues or bone. (medscape.com)

The ability of osteoprogenitor cells to regenerate bone was significantly reduced upon pretreatment with PDGF-BB in vitro. (allenpress.com)
As demonstrated in previous studies many approaches have been applied to stimulate angiogenesis in the process of bone healing including the combination with endothelial cells the application of vascular growth factors vascular redesigning by microsurgery and gene transfection techniques [14-17]. (researchassistantresume.com)
They are derived from an osteoclast-monocyte lineage determined primarily by expression of CD68, a marker for monocytic precursor cells. (wikipedia.org)
this distinguishes the lesion from other osteogenic lesions which commonly have (benign) osteoclast-type giant cells. (wikipedia.org)
More osteogenic differentiation potential was observed for both cells under dynamic culture in a perfusion bioreactor compared with static culture. (utwente.nl)
Thus, the objective of the scientific initiation project is to study the possible role of the BMP2-Smad1/5/8 signaling pathway in differentiating of human pulp cells in osteogenic phenotype induced by CsinCPI-2. (fapesp.br)
The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
specifically, enamel is deposited by ameloblasts derived from the epithelium, while dentin is deposited by odontoblasts derived from mesenchymal cells. (ispub.com)
In the bone marrow, AAV9-NEM transduced Cd31, Cd34, and Cd90 expressing cells at a higher percentage than unmodified AAV9. (nature.com)
In vitro, these MAbs recognize cell surface antigens present on two additional cell lines, ROS 24/1 and Rat 2 cells, and on the adherent cell population cultured from rat long bone marrow. (docksci.com)
This latter cell line is derived from the same rat osteosarcoma but is fibroblastic in morphology and expresses none of the OB-like characteristics of ROS 17/2.8 cells. (docksci.com)
Recently, intercellular communication between bone cells and surrounding cells has been gradually recognized, and macrophages on the surface of bone have been proven to regulate bone metabolism. (researchpad.co)
Recently, our understanding of the bone system has moved beyond bone cells. (researchpad.co)
The crosstalk between bone cells and surrounding cells as well as the relationship between the bone and bone microenvironment has been gradually recognized and regarded as a relatively important factor in bone metabolism ( 9 ). (researchpad.co)
These cells have a relatively short-lifespan, estimated at 3 months in human bones [ 5 ]. (biomedcentral.com)
Osteocytes, which are the most abundant cells in bone, composing 90-95% of all bone cells in an adult. (biomedcentral.com)
Further, Fgfr3 + endosteal stromal cells give rise to aggressive osteosarcoma-like lesions upon loss of p53 tumor suppressor through unregulated self-renewal and aberrant osteogenic fates. (nature.com)
The term "transitional" emphasizes the unique feature of these cells that are particularly abundant in the young bone marrow. (nature.com)
These cells are found as a closely packed, sheath of cells on the surface of the bone. (pediaa.com)
The four types of bone cells and their functions are shown in figure 1 . (pediaa.com)
Osteoclasts are derived from circulating cells such as monocytes and macrophages. (pediaa.com)
Following standard protocols cells were stimulated lineage specific towards the osteogenic and chondrogenic lines. (biomedcentral.com)
Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)

The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
We have recently demonstrated that stromal cell-derived factor-1? (omicsdi.org)
Increasing evidence suggests that stromal cell-derived factor-1 (SDF-1) is involved in bone formation. (omicsdi.org)
Stromal cell-derived factor-1? (omicsdi.org)

The study evaluates the ability of osteoprogenitors treated with platelet-derived growth factor BB (PDGF-BB) delivered on vinyl styrene microbeads (VSM) to regenerate rat calvarial critical-size defects (CSDs). (allenpress.com)
however, we hypothesize that the VSM can be used to deliver osteoblastic progenitors into bone defects. (allenpress.com)
Background: Reconstruction of bone fractures and defects remains a big challenge in orthopedic surgery. (uconn.edu)
Several biomaterials such as metal alloys, ceramics or bone cements have been used for decades as permanent implants to overbridge or stabilize bone defects. (biomedcentral.com)
Large bone defects resulting from trauma, congenital defects, neoplasm, failed arthroplasty, and infection are quite common [ 1 , 2 ], and the incidences of nonunion and delayed union are very high [ 3 ]. (hindawi.com)
It remains a great challenge for orthopedic surgeons to achieve osseous reconstruction for nonunion and bone defects. (hindawi.com)

As conventional pharmacological therapy options for osteoporosis are often associated with severe side effects, bone grafts are still considered the clinical gold standard. (upmbiomedicals.com)
In addition, bone grafts are frequently used for spinal fusion, joint revision surgery, corrective osteotomy and bone reconstruction. (biomedcentral.com)

In contrast, cultivation on flat titanium and on membranes equipped with 100 nm pores was not sufficient to induce osteogenic differentiation. (uni-bielefeld.de)

It is distinguished by pathologic loss of alveolar bone and the periodontal ligament (PDL), thus causing tooth mobility and even adult tooth loss [ 1 ]. (biomedcentral.com)
In conclusion, AT-MSC present higher osteogenic potential than BM-MSC when extracted from adult dogs. (scielo.br)
Alcohol: a simple nutrient with complex actions on bone in the adult skeleton. (springer.com)
The neurocranium (calvaria in the adult) and viscerocranium (facial bones and portions of cranial base in the adult) combine to form the skull and grow independently through separate mechanisms. (medscape.com)
Slightly more rostral, the hypophyseal cartilages coalesce to form the body of the adult sphenoid bone. (medscape.com)
Trabeculae cranii fuse to form the body of the adult ethmoid bone. (medscape.com)

BMSCs, augments the cell-mediated therapeutic effects resulting in enhanced bone formation, as evidenced by ex vivo ?CT and bone histomorphometry. (omicsdi.org)
but the overall therapeutic process lasts for a relatively long period, and a variety of complications may arise, such as pin loss, infection around the transmucosal pin, bone fracture, and restriction in joint motion [ 8 ]. (hindawi.com)

Bone mineral density (BMD) of the whole-body, proximal femur and lumbar vertebrae was measured by dual-energy X-ray absorptiometry (DXA). (frontiersin.org)
accordingly, a decrease in bone mineral density occurs. (biomedcentral.com)
As a consequence, bone aging is the main risk factor for primary osteoporosis, characterized by a reduction in bone mineral density, predisposing the elderly population to an increased risk of fractures. (biomedcentral.com)
Bone mineral density and bone mineral content of the TPF-treated mice were significantly increased compared to the control mice. (biomedcentral.com)
Alcohol consumption and bone mineral density in elderly women. (springer.com)

Furthermore, intraperitoneal administration of GSK4112, a Rev-erb agonist, protects RANKL-induced bone loss via inhibition of osteoclast differentiation in vivo . (molcells.org)
augments bone formation in vivo in a model of local BMSC transplantation following irradiation. (omicsdi.org)
However, the effects of the TPF on in vivo bone formation remain unclear. (biomedcentral.com)
In this study, we investigated the effects of the TPF on in vivo bone formation, injected the TPF (20 mg/kg) twice a day in the low calcium diet mice and killed them after 21 day. (biomedcentral.com)

The orderly progress of bone reconstruction is a complex process that involves many factors, including enzymes, hormones, and cytokines ( 2 ). (researchpad.co)

However, axial views of the subarticular bone (bony area adjacent to the articular cartilage) is not accurate due to voxel signal averaging. (wikipedia.org)
In addition, development and genetic studies have shown that GDF-5 null mutation or transgenic mice exhibit abnormal growth patterns or overgrowth of limbs, long bones, cartilage, joints and digits (Storm et al. (ispub.com)
Parachordal cartilage, located at the end of the notochord, combines with occipital somites to form the base of the occipital bone, later growing to boundaries of the foramen magnum. (medscape.com)

Aging is a high risk factor for the development of osteoporosis, a multifactorial age-related progressive disease characterized by reduced bone mass and increased risk of fractures. (biomedcentral.com)

To evaluate whole-body vibration (WBV) osteogenic potential in physically inactive postmenopausal women using high-frequency and combined amplitude stimuli. (frontiersin.org)
Bone has the potential for spontaneous healing. (omicsdi.org)
Our findings point towards the stimulation of bone formation by TPF, suggested that the TPF could be a potential natural anabolic agent to treat patients with bone loss-associated diseases such as osteoporosis. (biomedcentral.com)
The application of allograft bone as an alternative treatment option carries the potential risk of infection and graft failure as a consequence of the reduced osteoinducitvity of allograft bone [ 4 ]. (biomedcentral.com)

Conclusion: The production of surface-level charge from ultrasonic stimulation of PLLA and the use of that charge to promote osteogenic differentiation in ADSCs was successfully demonstrated. (uconn.edu)

Thus, BMPs have been developed as bone anabolic agents and approved for clinical use [ 6 ]. (biomedcentral.com)

Anatomy44

Organisms4

Diseases20

Chemicals and Drugs74

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes32

Disciplines and Occupations1

Technology, Industry, Agriculture10

Information Science2

Morphogenetic12

Osteoblasts25

MSCs21

Adipogenic5

Osteopontin2

Osteonectin2

Sialoprotein1

Tissue27

Promote the osteogenic differentiation2

Osteogenesis12

Runx21

Scaffolds8

Chondrogenic differentiation1

Regeneration13

Proliferation11

Osterix2

Adipose-derived stem5

Osteoinductive6

Collagen type2

Alkaline phosphatase activity1

Resorption11

Protein3

Stem15

Osteoblast10

Scaffold1

Mineralization1

Markers3

ASCs4

Dental pulp2

Gene expression1

Autologous bone grafts1

Skeletal3

Mesenchymal cell1

Tissues4

Cells23

Stromal4

Defects6

Grafts2

Induce1

Adult6

Therapeutic2

Mineral5

Vivo4

Reconstruction1

Cartilage3

Fractures1

Potential4

ADSCs1

BMPs1