• There is no true consensus among laboratories in terms of a reference range of values for the osmotic fragility of erythrocytes. (medscape.com)
  • The susceptibility of osmotic lysis of erythrocytes is a function of surface area to volume ratio. (medscape.com)
  • At times, the result of the osmotic fragility test may be expressed as the concentration of NaCl in solution that causes 50% hemolysis of erythrocytes. (medscape.com)
  • The osmotic fragility test can be performed on freshly drawn blood (within 2 hours from collection), but some laboratories incubate collected samples at 37°C for 24 hours to improve the sensitivity of the test, since a greater extent of osmotic lysis is noted for abnormal erythrocytes than normal ones. (medscape.com)
  • In a disease such as hereditary spherocytosis, erythrocytes have a smaller ratio of surface area to volume and are thus more susceptible to osmotic stress, as opposed to the increased resistance characteristic of thalassemia, iron deficiency anemia, or any other condition that would cause an increased surface area-to-volume ratio. (medscape.com)
  • The affected erythrocytes have increased osmotic fragility. (wikipedia.org)
  • The temperature dependence of osmotic fragility in calf and adult cattle erythrocytes was compared. (eurekamag.com)
  • This enzyme defect disrupts heme biosynthesis and leads to an accumulation of uroporphyrin in erythrocytes, which, in turn, increases their osmotic fragility and results in hemolysis. (standardofcare.com)
  • The effects of eleven flavonoids on lipid peroxidation, protein degradation, deformability and osmotic fragility of human erythrocytes exposed in vitro to 10 mM H 2 O 2 for 60 min at 37˚C have been studied. (scirp.org)
  • Quercetin and 3,5,7-trihydroxy-4'-methoxy flavone-7-rutinoside significantly protected erythrocytes against loss of deformability and increased osmotic fragility, indicating that the loss of erythrocyte deformability and the increase in osmotic fragility of erythrocytes exposed to H 2 O 2 are related to protein degradation rather than to lipid peroxidation. (scirp.org)
  • In vitro exposure of erythrocytes to oxygen radical generating systems (such as H 2 O 2 , ascorbate/Fe 3+ , cumene hydroperoxide, tert-butyl hydroperoxide, etc.) was shown to induce lipid peroxidation, protein degradation, loss of deformability, an increase in osmotic fragility, membrane lipid bilayer perturbation, inhibition of enzymes and hemolysis [5-12]. (scirp.org)
  • The osmotic fragility test (OFT) is used to measure erythrocyte resistance to hemolysis while being exposed to varying levels of dilution of a saline solution. (medscape.com)
  • Below the arrangement of tubes onset of hemolysis is at 0.85% of Nacl and complete hemolysis at 0.58% of NaCl, therefore increased fragility than above. (medicalbasic.net)
  • Effectiveness of one tube osmotic fragility screening in detecting beta-thalassaemia trait. (bmj.com)
  • Osmotic fragility test of RBCs. (medicalbasic.net)
  • Stored RBCs develop complex and multiple membrane changes leading to increased osmotic fragility (due to shape change), decrease in the level of 2,3-DPG (increasing the oxygen affinity of haemoglobin), reduced ATP levels, loss of potassium ions with seeping in of sodium ions, loss of deformability of RBCs and reduced life span. (ndtv.com)
  • In this study, we investigated if the osmotic tolerance of RBCs of tegu lizard under hibernation is different from the cells obtained from animals while normal activity. (bvsalud.org)
  • Our findings suggest that RBCs are more fragile during the hibernation period, although we did not find evidence of an oxidative stress scenario associated with the accentuated fragility. (bvsalud.org)
  • Fetal red blood cells (RBCs) obtained during in utero transfusion had extremely abnormal osmotic fragility. (psu.edu)
  • Results are then plotted against the NaCl concentrations, yielding an osmotic fragility curve that is then compared to obtained normal control values. (medscape.com)
  • Osmotic gradient ektacytometry: A valuable screening test for hereditary spherocytosis and other red blood cell membrane disorders. (cdc.gov)
  • The osmotic fragility test is mainly used in helping with the diagnosis of hereditary spherocytosis but it is also used in some countries as a method to screen for beta thalassemia, especially where laboratory resources are limited. (medscape.com)
  • A maternal history of mild autosomal dominant hereditary spherocytosis was present, and the father, who was hematologically normal, had a slightly abnormal osmotic fragility test. (psu.edu)
  • Molecular diagnosis of hereditary spherocytosis by multi-gene target sequencing in Korea: matching with osmotic fragility test and presence of spherocyte. (cdc.gov)
  • A microtechnique for measuring red cell osmotic fragility of infants. (bmj.com)
  • Flow Cytometric Analysis of Red Blood Cell Osmotic Fragility. (medscape.com)
  • Red cell fragility can be helpful in establishing the functional state of RBC. (medicalbasic.net)
  • Cell wall effects were supported by gene disruption and osmotic fragility experiments. (st-andrews.ac.uk)
  •  3.4.1.1 Low dosage CATT3: Computer-Aided Thermodynamic Tables (CATT) for Mac .Determination of the area under the concentration time curve for erythrocyte osmotic fragility in human blood. (enricmcatala.com)
  • Walski T, Chludzinska L, Komorowska M, Witkiewicz W. Individual osmotic fragility distribution: a new parameter for determination of the osmotic properties of human red blood cells. (medscape.com)
  • Parpart AK, Lorenz PB, Parpart ER, Gregg JR, Chase AM. The osmotic resistance (fragilità) of human red cells. (medscape.com)
  • Some researches have shown that melatonin (MLT) has effects on the erythrocyte deformability and on the osmotic fragility which, currently, seem to be heterogeneous and fragmentary. (nel.edu)
  • They characterized vitamin C concentrations from human and mouse red blood cells and plasma, and showed an inverse relationship between red blood cell vitamin C concentrations and deformability, measured by osmotic fragility. (nih.gov)
  • The absence of the Rh complex alters the RBC shape, increases its tendency to break down (osmotic fragility), and shortens its lifespan, resulting in a hemolytic anemia that is usually mild. (nih.gov)
  • A complete clinical history and biochemical investigations, including thyroid function tests namely, thyroid -stimulating hormone (TSH) and free T4 (fT4), RBC membrane osmotic fragility test by gradient concentration of NaCl, and RBC membrane peroxidation using thiobarbituric acid reactive substances (TBARS) tests were performed among thirty two hypothyroidism patients and healthy controls. (niscair.res.in)
  • The osmotic fragility test is mainly used in helping with the diagnosis of hereditary spherocytosis but it is also used in some countries as a method to screen for beta thalassemia , especially where laboratory resources are limited. (medscape.com)
  • Often genetic disorders like Spherocytosis or Thalassemia diagnosis are conducted via the process of osmotic fragility test. (flebo.in)
  • The underlying cause behind the fragility of the red blood cells is that the mutations caused by spherocytosis make the RBC cell membrane rigid and that decreases its flexibility, causing it to break apart easily. (flebo.in)
  • Comparison of a modified flow cytometry osmotic fragility test with the classical method for the diagnosis of hereditary spherocytosis. (bvsalud.org)
  • Previously, we reported the genetic background of hereditary spherocytosis, which can be distinguished by morphological characteristics of RBCs and osmotic abnormalities of RBC membranes 10 . (nature.com)
  • Osmotic gradient ektacytometry (OGE) is currently considered the gold standard for the diagnosis of red cell membrane disorders, especially hereditary spherocytosis (HS). (mdpi.com)
  • Kattamis C, Efremov G, Pootrakul S. Effectiveness of one tube osmotic fragility screening in detecting beta-thalassaemia trait. (medscape.com)
  • Typical laboratory findings in EPB42-HS include anemia (decreased hemoglobin [Hgb] level) and reticulocytosis (increased percentage of reticulocytes), with high mean corpuscular Hgb concentration, presence of spherocytes in the peripheral blood smear, significantly decreased or absent haptoglobin, mildly increased osmotic fragility in osmotic fragility assay, increased O min (osmolality at which 50% of red blood cells hemolyze), and decreased maximal elongation index (EI max ) in osmotic gradient ektacytometry. (nih.gov)
  • or intravascular osmotic lysis following the generation of terminal membrane attack complexes of the complement pathway. (vin.com)
  • Ascorbic acid supplementation also abolished the long tail of the erythrocyte osmotic fragiligram and increased the resistance of the cells to lysis. (unboundmedicine.com)
  • The osmotic fragility test (OFT) is used to measure erythrocyte resistance to hemolysis while being exposed to varying levels of dilution of a saline solution. (medscape.com)
  • Parpart AK, Lorenz PB, Parpart ER, Gregg JR, Chase AM. The osmotic resistance (fragilità) of human red cells. (medscape.com)
  • The present study identified, quantified, and characterized the combinations of phytocomponents from fractionated leaf extracts of Anacardium occidentale , Psidium guajava , and Terminalia catappa that stabilized sickle erythrocyte membrane against osmotic stress, or otherwise, using standard chromatographic-spectrophotometric techniques, namely GC-MS, FT-IR, and UV-visible systems. (springeropen.com)
  • The effect of ascorbic acid supplementation (100 mg/day for 6 weeks) on blood pressure, packed cell volume, irreversibly sickled cells, per cent fetal hemoglobin, hemoglobin concentration, and erythrocyte osmotic fragility was assessed in children suffering from sickle cell anemia. (unboundmedicine.com)
  • 8. Protective effect of gallic acid on alloxan-induced oxidative stress and osmotic fragility in rats. (nih.gov)
  • Other laboratory tests (eg, stool testing, incubated osmotic fragility testing, measurement of lead in tissue, and bone marrow aspiration) are useful for establishing the etiology of iron deficiency anemia and for excluding or establishing a diagnosis of 1 of the other microcytic anemias. (medscape.com)
  • People suffering from anemia too are prescribed to opt for the osmotic fragility test to understand the exact causal effect for the patient to become anemic. (flebo.in)
  • In conclusion, the results indicated that RBC osmotic fragility increases significantly in hypothyroidism possibility due to increased membrane lipid peroxidation as observed in the study. (niscair.res.in)
  • Patients are diagnosed via the osmotic fragility test, and treatment includes folic acid supplementation, and eventually splenectomy. (picmonic.com)
  • Slightly increased osmotic fragility was present, and bone marrow biopsy reported a hypercellular marrow with predominance of erythroid precursors. (hindawi.com)
  • With osmotic fragility, the test results can either be negative or positive. (flebo.in)
  • Scaglione, L. 2007-05-03 00:00:00 The osmotic fragility and erythrocyte size were studied on 80 blood samples (40 of each) obtained from both genusCaiman species present in Argentina, maintained in captive condition in the Northeastern region of the country (Province of Corrientes). (deepdyve.com)
  • This work wished to evaluate in vitro the possible modifying action of MLT at pharmaceutical doses on the osmotic fragility of rat blood samples treated with cytochalasin B (CB). (nel.edu)
  • Walski T, Chludzinska L, Komorowska M, Witkiewicz W. Individual osmotic fragility distribution: a new parameter for determination of the osmotic properties of human red blood cells. (medscape.com)
  • The osmotic fragility test subjects the blood sample to stress by adding the samples to solutions with different salinity levels. (flebo.in)
  • High mean erythrocyte fragility values were common in infected cattle groups. (biomedcentral.com)
  • Certain hereditary conditions call for the osmotic fragility test. (flebo.in)