DiseasesChemicals and Drugs
Orotate PhosphoribosyltransferaseOrotidine-5'-Phosphate DecarboxylaseOrotic AcidPentosyltransferasesPhosphoribosyl PyrophosphateDihydroorotate OxidaseHypoxanthine PhosphoribosyltransferaseAzauridineAdenine PhosphoribosyltransferaseUridine PhosphorylaseCarboxy-LyasesDihydroorotaseUracilAspartate CarbamoyltransferaseNicotinamide PhosphoribosyltransferaseLesch-Nyhan SyndromePyrimidinesATP PhosphoribosyltransferaseHypoxanthinesAnthranilate Phosphoribosyltransferase
PyrE2.10OPRTOrotidineUracil phosphoribosyltransferasePyrimidine nucleotidesSynthaseEnzymeRiboseMoleculeOrotidylatePRPPPyrophosphateResiduesProteinTransitionActivityDihydropyrimidine dehydrogenaseUridineOPRTaseOrotidine-5-monophosphaPhosphoribosyl transferaseOrotic acidDecarboxylaseInhibitorsReactionActive
PyrE2
  • 2.1 angstrom resolution crystal structure of an orotate phosphoribosyltransferase (pyre) from vibrio cholerae o1 biovar eltor str. (edu.pl)
  • d) pMTL-MPD24: positive control for cargo delivery at the pheA locus through carriage of the FAST reporter gene flanked by the thiolase ( thl ) promoter (P thl ) and pyrE-hydA (orotate phosphoribosyltransferase and hydrogenase I) terminator from Clostridium acetobutylicum . (plasmidvectors.com)
2.101
OPRT2
  • We have previously shown that orotate phosphoribosyltransferase (OPRT) and orotidine 5'-monophosphate decarboxylase (OMPDC) in human malaria parasite Plasmodium falciparum form an enzyme complex, containing two subunits each of OPRT and OMPDC. (unboundmedicine.com)
  • Segments of the human UMP synthase cDNA coding for the orotate phosphoribosyl transferase (OPRT) and orotidylate decarboxylase (ODC) domains of the bifunctional protein UMP synthase were produced by polymerase chain reaction techniques and cloned into a eukaryotic expression vector. (unboundmedicine.com)
Orotidine5
  • It catalyzes the formation of orotidine 5'-monophosphate (OMP) from orotate and phosphoribosyl pyrophosphate. (wikipedia.org)
  • The enzyme Uridine monophosphate synthase (EC 4.1.1.23, UMPS) (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) catalyses the formation of uridine monophosphate (UMP), an energy-carrying molecule in many important biosynthetic pathways. (wikipedia.org)
  • After addition of ribose-P to orotate by OPRTase to form orotidine-5'-monophosphate (OMP), OMP is decarboxylated to form uridine monophosphate by ODCase. (wikipedia.org)
  • Uridine monophosphate is the enzyme that catalyzes orotate phosphoribosyltransferase and orotidine-5 ′ -monophosphate decarboxylase reactions. (msdmanuals.com)
  • After the pyrimidine ring forms, 5-phospho-α-D-ribosyl 1-pyrophosphate (PRPP), a ribose phosphate, reacts to orotate to form orotidine-5-monophosphate (OMP). (biologyonline.com)
Uracil phosphoribosyltransferase1
  • Uracil phosphoribosyltransferase in the cytoplasm provides the major pyrimidine salvage for the parasite. (tmu.edu.tw)
Pyrimidine nucleotides2
  • The anaerobic parasitic protozoa Tritrichomonas foetus is found incapable of de novo pyrimidine biosynthesis by its failure to incorporate bicarbonate, aspartate, or orotate into pyrimidine nucleotides or nucleic acids. (tmu.edu.tw)
  • As a phosphoribosyltransferase is … metabolism of purine & pyrimidine nucleotides participate in many organisms carbamoyl phosphate is by. (euroasfalti.net)
Synthase2
  • In yeast and bacteria, orotate phosphoribosyltransferase is an independent enzyme with a unique gene coding for the protein, whereas in mammals and other multicellular organisms, the catalytic function is carried out by a domain of the bifunctional enzyme UMP synthase (UMPS). (wikipedia.org)
  • Orotate phosphoribosyltransferase is located at the N-terminal domain of UMP synthase. (wikipedia.org)
Enzyme1
  • Orotate phosphoribosyltransferase (OPRTase) or orotic acid phosphoribosyltransferase is an enzyme involved in pyrimidine biosynthesis. (wikipedia.org)
Ribose1
  • This process happens in multiple steps with orotate phosphoribosyltransferase responsible for the first step of adding a ribose ring to orotate. (wikipedia.org)
Molecule1
  • Transition state analyses have used isotopic effects and quantum calculations to reveal a completely dissociated dianionic orotate structure, a ribocation, and a nucleophilic pyrophosphate molecule. (wikipedia.org)
Orotidylate1
  • The reaction of orotic acid (orotate) to orotidylate is catalyzed by orotate phophoribosyltransferase with the cofactor PRPP, which is a cofactor commonly used for nucleotide synthesis. (wikipedia.org)
PRPP2
  • Two main interactions attract PRPP to assist orotate phophoribosyltransferase in this reaction. (wikipedia.org)
  • First, orotate phophoribosyltransferase has Aspartic acid- Aspartic acid residues next to its PRPP-binding motif which interact with the ribosyl 2-/3- hydroxyl groups that stabilize the movement of Carbon-1 of the bound ribosyl group. (wikipedia.org)
Pyrophosphate1
  • When orotate is present, pyrophosphate binding affinity is increased fourfold and the reaction undergoes burst kinetics, with rapid phosphoribosyl transfer and then slow release of products. (wikipedia.org)
Residues1
  • The N-terminal has a pair of antiparallel strands, with residues that interact with bound orotate and Lys 26 that extends to the active site and forms a bond with the flexible loop in its closed form. (wikipedia.org)
Protein2
  • The protein has two type I phosphoribosyltransferase folds, related by 2-fold pseudosymmetry. (biomedcentral.com)
  • Lastly , the dihydroorotate is oxidized by dihydroorotate dehydrogenase (an integral membrane protein in the inner mitochondrial membrane ) to convert into orotate . (biologyonline.com)
Transition1
  • Pyrimidine phosphoribosyltransferases such as orotate phosphoribosyltransferase activate their substrates by forming SN1-like transition states, facilitating migration of the ribosyl anomeric carbon region to MgPPi. (wikipedia.org)
Activity2
  • Orotate PRTase activity is activated by low concentrations of OMP, phosphate, and ADP. (wikipedia.org)
  • METHODS AND RESULTS: The pyrimidine biosynthetic pathway enzymes were measured in cell extracts from P. resinovorans ATCC 14235 and from an auxotroph lacking orotate phosphoribosyltransferase activity. (bvsalud.org)
Dihydropyrimidine dehydrogenase1
  • 26. Expression of thymidylate synthase, orotate phosphoribosyltransferase and dihydropyrimidine dehydrogenase in thymic epithelial tumors. (nih.gov)
Uridine3
  • The enzyme Uridine monophosphate synthase (EC 4.1.1.23, UMPS) (orotate phosphoribosyl transferase and orotidine-5'-decarboxylase) catalyses the formation of uridine monophosphate (UMP), an energy-carrying molecule in many important biosynthetic pathways. (wikipedia.org)
  • After addition of ribose-P to orotate by OPRTase to form orotidine-5'-monophosphate (OMP), OMP is decarboxylated to form uridine monophosphate by ODCase. (wikipedia.org)
  • Uridine monophosphate is the enzyme that catalyzes orotate phosphoribosyltransferase and orotidine-5 ′ -monophosphate decarboxylase reactions. (msdmanuals.com)
OPRTase2
Orotidine-5-monophospha2
  • It catalyzes the formation of orotidine 5'-monophosphate (OMP) from orotate and phosphoribosyl pyrophosphate. (wikipedia.org)
  • The last two enzymes of the pathway, orotate phosphoribosyltransferase and orotidine-5-monophosphate decarboxylase, are fused in a multi-domain enzyme and are duplicated in some P. infestans strains. (unboundmedicine.com)
Phosphoribosyl transferase2
  • The individual orotate phosphoribosyl transferase and orotidine monophosphate domains have been well characterized, but little is known about the overall structure of the protein and how the organization of domains impacts function. (bvsalud.org)
  • Our analysis suggests that the transition between dimer and multimer is driven primarily by oligomerization of the orotate phosphoribosyl transferase domain. (bvsalud.org)
Orotic acid1
  • The reaction of orotic acid (orotate) to orotidylate is catalyzed by orotate phophoribosyltransferase with the cofactor PRPP, which is a cofactor commonly used for nucleotide synthesis. (wikipedia.org)
Decarboxylase1
  • Fluoroorotic acid (FOA) has long been used to inhibit orotidlyate decarboxylase or orotate phosphoribo- syltransferase, encoded by URA3 and URA5, respectively. (forextrading-madeeasy.com)
Inhibitors1
  • There trigger inhibitors that NOTCH2 long is with orotate small psychosis 1-beta( HNF1B) in ability electromyography( Massa et al. (evakoch.com)
Reaction2
  • Two main interactions attract PRPP to assist orotate phophoribosyltransferase in this reaction. (wikipedia.org)
  • Orotate phosphoribosyltransferase has a core part plus a flexible loop, which when closed prevents solvent from entering during reaction. (wikipedia.org)
Active2
  • This slow release is thought to be due to the solvent-exposed loop of orotate phosphoribosyltransferase that protects the active site during the first step. (wikipedia.org)
  • The N-terminal has a pair of antiparallel strands, with residues that interact with bound orotate and Lys 26 that extends to the active site and forms a bond with the flexible loop in its closed form. (wikipedia.org)