Organization membrane separation liquid phases. Medical search. Frequent questions

The contributors discuss the 3D organization of chromatin, the various nuclear bodies and compartments that have been identified, and the roles of RNA and actin in shaping nuclear organization, as well as how these structures interact with each other and with peripheral features (e.g., the nuclear pore complex and inner nuclear membrane proteins) to carry out the work of the nucleus. (cshlpress.com)
Coacervate / condensate phase of polyelectrolytes and proteins. (aalto.fi)
For protein systems, we focus on block proteins that undergo liquid-liquid phase separation (LLPS) as a preassembly step for structural protein materials. (aalto.fi)
However, constructing such molecular systems is a challenge because of the difficulties in working with membrane proteins, very hydrophobic molecules that are prone to aggregate. (uni-muenchen.de)
In this way, a hydrophobic environment was supplied to the membrane proteins to fold and function to interact with the extravesicular environment. (uni-muenchen.de)
By assessing the functions of membrane proteins with a fluorescence-activated cell sorter, the artificial cell could evolve based on the function of the membrane proteins. (uni-muenchen.de)
GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane. (mpg.de)
Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains. (mpg.de)
To form these phase-separated compartments, HCMV uses its UL112-113 proteins. (leibniz-interact.de)
In the study now published in Cell Reports , the research team led by Prof. Wolfram Brune (HPI) and Prof. Jens B. Bosse (MHH, CSSB, HPI) used live-cell imaging and photo-oligomerization methods to show that the HCMV proteins UL112-113 undergo liquid-liquid phase separation, which supports the formation of replication compartments in the nucleus. (leibniz-interact.de)
Phase separation by the UL112-113 proteins is not only important for the spatial organization of HCMV pre-replication compartments, but also essential for the recruitment of proteins for viral DNA replication," Prof. Jens B. Bosse, head of the HPI-associated Quantitative Virology group, comments on the results. (leibniz-interact.de)
Studying the architecture, conformational dynamics and interactions of proteins that undergo liquid-liquid phase separation. (leeds.ac.uk)
Microdomains or lipid rafts greatly affect the distribution of proteins and peptides in the membrane and play a vital role in the formation and activation of receptor/protein complexes. (frontiersin.org)
Our simulation experiments indicate, that for receptor/raft ratios smaller than 1, i.e., when the number of raft compartments clearly exceeds the number of pathway specific membrane proteins, we observe significant decrease in LRP6 phosphorylation and downstream pathway activity. (frontiersin.org)
It is known that GSLs interact with both intracellular as well as exogenous proteins and are critical for membrane organisation, signalling, and recognition events. (springer.com)
Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
2011 ) have reviewed the processes by which matrix and membrane proteins are incorporated into the peroxysome. (tcdb.org)
In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)
The membrane bound docking subcomplex includes several Pex proteins, Pex13p, 14p and 17p. (tcdb.org)
Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)
Video microscopy technology resolving single molecule movement was employed to monitor the dynamic interaction of membrane-associated signalling proteins with TCR signalling clusters in plasma membrane domains. (biomedcentral.com)
These studies tracked the movement of Lck and LAT in the T cell plasma membrane and showed retention of these proteins in the submicron TCR signalling domains and their subsequent release. (biomedcentral.com)
Numerous intracellular signalling proteins interact with plasma membrane lipids of the cytoplasmic leaflet via specific lipid headgroup-binding domains. (biomedcentral.com)
While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)
Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)

In particular, the impact of α-spinasterol on the structure and organization of lipid membranes was investigated and compared with those of cholesterol. (mdpi.com)
STED microscopy detects and quantifies liquid phase separation in lipid membranes using a new far-red emitting fluorescent phosphoglycerolipid analogue. (mpg.de)

We are aiming at characterizing the signaling mechanisms that regulate the liquid-liquid phase transitions in the synaptic vesicle cycle, mitochondrial functions, and degradation pathways at nerve terminals to clarify how do they become affected at the early stages of neurodegenerative diseases and which molecular steps lead to protein aggregate formation. (ki.se)
In spite of the relevance of lipid-protein interactions, the phase behaviour of the lipid components of the cell membrane is believed to be of major importance to uncover some underlying principles behind the membrane function. (hindawi.com)
We encapsulated a reconstituted in vitro transcription-translation system inside a cell-sized phospholipid vesicle together with the DNA encoding membrane protein of interest. (uni-muenchen.de)
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane. (mpg.de)
Elegant studies have demonstrated Dvl-mediated formation of a membrane proximal "signalosome," which enables critical protein interactions at the PM ( 5 ). (rupress.org)
In our research we use a mass spectrometry-based integrative structural biology toolkit, and a combination of other biochemical and biophysical techniques, to elucidate the functional mechanisms of protein complexes, and characterise dynamic and transient assemblies involved in essential cellular functions, cellular organisation and disease. (leeds.ac.uk)
Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
Being typically a minor component of the cell membrane, they are of utmost importance for biological functions that rely on lipid-lipid and lipid-protein interactions (Schnaar and Kinoshita 2015 ). (springer.com)
Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)
Here we review new biophysical and biochemical data which provide an updated picture of the highly dynamic nanometer-sized cholesterol/sphingolipid-rich raft domains stabilised by protein-networks to form TCR signalling platforms in the T cell plasma membrane. (biomedcentral.com)
We will here discuss recent data which provide a detailed picture of the membrane raft characteristics of the plasma membrane domains supporting active TCR signalling protein complexes. (biomedcentral.com)
Three prizes were awarded to six researchers working across the life sciences on cellular organization, protein structure, and the genetic underpinnings of a chronic sleep disorder. (the-scientist.com)
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
Mounting evidence suggests that the genetic disorders/mutation and diseases change not only the protein expression patterns but also membranes themselves. (lu.se)

Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane. (ki.se)
STED-FLCS: An advanced tool to reveal spatiotemporal heterogeneity of molecular membrane dynamics. (mpg.de)
Molecular plasma membrane dynamics dissected by STED nanoscopy and Fluorescence Correlation Spectroscopy (STED-FCS). (mpg.de)
To connect microscopic dynamics to macroscale behaviors we measured the phase diagram of a new system of microtubule filaments and end-accumulating kinesin molecular motors. (aps.org)
With this Dynamics workshop, our goal is to bring together leading experts in the field of membrane dynamics, with a particular focus on neutrons and X-rays, but also complementary techniques including NMR, single molecule spectroscopy and computer modeling. (lu.se)

Swi6 - an HP1 orthologue in Schizosaccharomyces pombe (S. pombe) - can both undergo liquid-liquid phase separation (LLPS) and compact nucleosomes in vitro [Sanulli, 2018]. (emerging-researchers.org)
We hypothesize that swi6-sm1, an allele of Swi6, may be such a separation-of-function allele, as the mutations that comprise the swi6-sm1 allele are in the N-terminal extension, a region of Swi6 implicated in its LLPS activity [Larson, 2017]. (emerging-researchers.org)
Ultimately, we hope to understand the role of LLPS in chromatin organization and its potential contribution to the integrity of the nuclear envelope. (emerging-researchers.org)
Recent studies found Axin ( 6 ) and the DC ( 7 ) undergo liquid-liquid phase separation (LLPS) and drive DC assembly. (rupress.org)
However, in the cytoplasm and nucleus, membrane-less organelles (MLOs) form by liquid-liquid phase separation (LLPS). (leeds.ac.uk)
Pex13 undergoes liquid-liquid phase separation (LLPS) with Pex5-cargo. (tcdb.org)

Both techniques give consistent phase diagrams, which compare well with literature results, showing their capability to map the phase behaviour of pure lipids as well as lipid mixtures. (hindawi.com)
During the last decades, the approach to overcome the complexity of biological membranes is to use simplified biomimetic models mainly consisting of binary or ternary mixtures of lipids [ 1 , 2 ]. (hindawi.com)
One of the most debated issues in the biophysics of lipids is lipid miscibility and its relevance to the function and organization of biological membranes. (hindawi.com)

Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes. (mpg.de)
The analysis of detergent-resistant T cell membranes had initiated the concept that early TCR signalling steps take place in cholesterol/sphingolipid-rich raft domains of T cell plasma membranes [ 10 ] (see Box for an overview of the current perception of raft domains in cell membranes). (biomedcentral.com)

Liquid-liquid phase separation in cells has emerged as a common principle for the organization of membrane-less compartments. (ki.se)
In nerve terminals in the central nervous system, liquid-liquid phase separation has been implicated in the organization or assembly of several functional compartments. (ki.se)
A research team from the Leibniz Institute for Experimental Virology (HPI) and the Centre for Structural Systems Biology (CSSB) has now shown that the formation of phase-separated compartments around viral genomes is necessary to recruit the viral DNA polymerase. (leibniz-interact.de)
These phase-separated pre-replication compartments are necessary to recruit viral DNA polymerase for viral genome replication. (leibniz-interact.de)
Our results show that phase separation is crucial for the formation of pre-replication compartments and viral DNA replication. (leibniz-interact.de)
Many cellular compartments are enclosed by membranes, with channels, pores and transporters regulating their composition. (leeds.ac.uk)

https://doi.org/10.1083/jcb.202205069 ) show Dvl2 undergoes liquid-liquid phase separation to stabilize β-catenin by pulling Axin into its biomolecular condensate at the plasma membrane. (rupress.org)

These complexes assemble into submicron TCR signalling domains in the T cell plasma membrane which were first studied by confocal fluorescence microscopy at the contact zone of a T cell with TCR-activating glass coverslips. (biomedcentral.com)
Confocal fluorescence microscopy was employed to monitor the distribution of TCR signalling plasma membrane sites and to relate their distribution to that of clustered raft markers which show resistance to Triton X 100 detergent solubilisation. (biomedcentral.com)

A lipid bound actin meshwork organizes liquid phase separation in model membranes. (mpg.de)

Polymerization-induced phase separation (PIPS) is the occurrence of phase separation in a multicomponent mixture induced by the polymerization of one or more components. (wikipedia.org)
However, in most cases in nature and technology, liquid systems are multicomponent and multiphase, with phase transitions between the phases, and thus far from equilibrium. (balzan.org)
A successful quantitative description and one-to-one comparison between well-controlled experiments and theory and numerics can potentially be achieved for such systems, making it possible to identify the underlying principles, and to place these examples in the context of relevant applications of multicomponent and multiphase liquid systems. (balzan.org)
These developments are more than timely, as the relevance of physiochemical hydrodynamics of multicomponent and multiphase liquids has become increasingly important in facing challenges to mankind in the twenty-first century. (balzan.org)

Extrapolating/Translating these values to the membrane of a "normal sized" cell yields a raft abundance, that, by far, outnumbers the membrane-associated pathway components of most individual signaling pathway, such as receptor and kinases. (frontiersin.org)
For most individual signaling pathway, this quantitative point of view would imply, that rafts clearly outnumber the membrane-associated pathway components, such as receptor and kinases. (frontiersin.org)
The results clearly highlight the influence of the different fatty acids of the Gb 3 sphingolipids on the phase behaviour and the binding properties of Shiga toxin B subunits, even though the membranes were only doped with 5 mol% of the receptor lipid. (springer.com)
2018 ). Here, we will solely focus on the less recognized globosides and in particular the globoside Gb 3 , which is the membrane receptor for vero toxins. (springer.com)
The ATP-dependent dislocation of the PTS1 receptor from the peroxisomal membrane into the cytosol is mediated by the AAA peroxins Pex1p and Pex6p ( Platta et al . (tcdb.org)

From the molecular analysis inside the artificial cells, we have found that the properties and phase transitions of polymers confined to the artificial cell often differ from those in bulk systems. (uni-muenchen.de)
We have qualitatively explained these phenomena by considering the length-dependent membrane wettability of polymers. (uni-muenchen.de)

I am basically a Liquid chromatography and mass spectrometry specialist for the Bionanosience department. (basyc.nl)
Multifunctional Separation Mechanism on Poly(oxyethylene) Stationary Phases in Capillary Liquid Chromatography. (quantabiodesign.com)
Nano-flow liquid chromatography tandem mass spectrometry (nano-flow LC-MS/MS) is the mainstay in proteome research because of its excellent sensitivity but often comes at the expense of robustness. (nature.com)
In this article we focus on challenges and opportunities for greening chromatography, adsorption and other separation technologies currently used in the pharmaceutical industry. (ddw-online.com)
The technique of chromatography was invented by botanist Mikael Tswett in 1903, and originally used for the separation of plant pigments (8,9). (ddw-online.com)
Chromatography relies on the selective partition of different molecules between a stationary phase and a mobile phase, as depicted in Figure 1. (ddw-online.com)
Liquid chromatography, most commonly used for purification in the pharmaceutical industry (10), typically employs modified silica or polymer particles as a solid phase, with water, organic solvents, or a mixture of the two as a mobile phase. (ddw-online.com)
Analytical chromatography is typically performed using a stationary phase column of 4.6mm diameter and 25cm length at a mobile phase flow rate of about 1mL/min. (ddw-online.com)
In contrast, preparative chromatography is carried out using much larger columns, sometimes in excess of 1 metre diameter, with mobile phase flow rates of many litres per minute. (ddw-online.com)
High performance liquid chromatography (HPLC) is one of the most commonly used analytical techniques in pharma today10. (ddw-online.com)
A method of analysis of eight flavones using high-performance liquid chromatography (HPLC)-diode array detection mass spectrometry in root and aerial tissues of the medicinal plant Scutellaria baicalensis was developed by Christopher RH et al (2005).2 The identity of the analytes was confirmed using retention time UV-visible and mass spectral comparisons to commercial standards. (webmedcentral.com)
A high-speed counter-current chromatography (HSCCC) method was developed by Li H B et al (2005)3 for the preparative separation and purification of three bioactive flavonoids, namely, baicalein, wogonin and oroxylin A, from Scutellaria baicalensis Georgi. (webmedcentral.com)
Composition of pigments from four species of brown seaweeds (Phaeophyceae) collected from Panjang Island, Central Java, Indonesia, was investigated with spectroscopic method and reverse-phase high-performance liquid chromatography (RP-HPLC). (pdfslide.us)

In 1997, Simons and Ikonen renewed the interest in lipid phase studies, suggesting that lateral phase separation in biological membranes could be functionally important [ 3 ]. (hindawi.com)
Separation of dibromochloromethane and bromoform from biological samples is most often achieved by headspace analysis, purge-and-trap collection, solvent extraction, or direct collection on adsorbent resins. (cdc.gov)
Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
In my talk, I will show you some examples how such biological cues influence the dynamic properties of membranes. (lu.se)

The increase in molecular weight of the reactive component renders one or more components to be mutually immiscible in one another, resulting in spontaneous phase segregation. (wikipedia.org)
The current study reports data on the membrane properties of the phytosterol (3β,5α,22 E )-stigmasta-7,22-dien-3-β-ol (α-spinasterol), which represents an important component of argan oil and have not been investigated so far in molecular detail. (mdpi.com)
we tend to get especially excited about assembly phenomena, the influence of interfaces (surfaces), and external driving of the material self-organization via molecular and energy flows. (aalto.fi)
This non-equilibrium situation implies several simultaneous transport processes across the interfacial layer separating the condensed and the gas phases, which in turn can have consequences on the molecular organization in the interfacial layer. (lu.se)
To help answer this question, scientists at the University of Cambridge have been probing the spontaneous self-organization of a simple chiral amino acid, alanine, into regular molecular arrays on copper single-crystal surfaces. (nanowerk.com)

A total of 1187 participants from 14 hospitals completed the survey in two phases: September 2013-April 2014 and May 2015-April 2017. (pubfacts.com)

These membrane-less organelles emerge as round structures and increase in size over time. (leibniz-interact.de)
Debackere and Rappa (1994) have suggested that technological paradigms typically emerge in two phases: bootlegging and bandwagon. (hyle.org)

Even though LRP6 is homogenously distributed in the membrane and only a minor fraction is raft-associated, its localization to lipid rafts is vital for the activation of the LRP6 signalosome, hence wnt/β-catenin signaling. (frontiersin.org)
Lipid rafts are local assemblies of highly concentrated sphingolipids and cholesterol in the cell membrane. (frontiersin.org)
Considerable controversy arose over the concept that cholesterol/sphingolipid-rich rafts in the T cell plasma membrane serve as a platform for TCR signalling reactions. (biomedcentral.com)
This controversy was founded on the initial definition of rafts as detergent resistant membranes which later turned out to misrepresent many features of cell membrane organisation under physiological conditions. (biomedcentral.com)
The results led to a refined perception of membrane rafts which resolves the controversies. (biomedcentral.com)

In turn, only recently surface-sensitive techniques have explored the phase behaviour of lipid layers in contact with solid surfaces, such as supported lipid bilayers (SLBs) and supported layers of vesicles (SLVs). (hindawi.com)
We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)

From a fundamental viewpoint, the interest in lipid phase behaviour stems from the nature and the thermodynamics of phase transitions, the mechanisms behind lipid miscibility and lateral phase separation, and the existence of critical phenomena [ 8 ]. (hindawi.com)
Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure. (mpg.de)
Early T cell signalling reactions are embedded in the complex and dynamic lipid bilayer matrix of the T cell plasma membrane and are critically defined by their lateral compartmentalisation in plasma membrane domains [ 1 , 2 ]. (biomedcentral.com)
The methodology presented allows the amount of native membrane material in the nSLB to be precisely controlled and display a uniform lateral distribution. (lu.se)
HN - 2008 BX - Lateral Sinus MH - Atrial Septum UI - D054087 MN - A07.541.459.249 MS - The thin membrane-like muscular structure separating the right and the left upper chambers (HEART ATRIA) of a heart. (bvsalud.org)

However, according to various experimental measurements, approximately 25 to 35 % of the cell plasma membrane is covered by nanoscopic raft domains with diameters ranging between 10 to 200 nm. (frontiersin.org)
Raft-organisation was subsequently studied using a number of detergent-free experimental approaches. (biomedcentral.com)

ESCPE-1 sorting of NRP1 may therefore play a role in the intracellular membrane trafficking of NRP1-interacting viruses such as SARS-CoV-2. (bvsalud.org)

In system containing of self-assembling amphiphilic molecules or colloidal suspensions, there is then a possibility of transport-generated interfacial phase separation. (lu.se)
With these molecules in hand, it became possible to investigate the phase behaviour of liquid ordered/liquid disordered supported membranes doped with the Gb 3 species by means of fluorescence and atomic force microscopy. (springer.com)
In one of these, the self-organisation is driven by hydrogen bonding between the molecules, while the chiral centre has no discernable impact on the regular array. (nanowerk.com)

2023 ). Imaging fluorescence cross-correlation spectroscopy showed that cargo import correlates with transient focusing of GFP-Pex13 and GFP-Pex14 on the peroxisome membrane. (tcdb.org)

The phase transitions of binary lipid mixtures are studied by a combination of Peltier-element-based adiabatic scanning calorimetry (pASC) and quartz crystal microbalance with dissipation monitoring (QCM-D). pASC, a novel type of calorimeter, provides valuable and unambiguous information on the heat capacity and the enthalpy, whereas QCM-D is proposed as a genuine way of determining phase diagrams by analysing the temperature dependence of the viscosity. (hindawi.com)
This work can be considered as a departure point for further investigations on more complex lipid mixtures displaying relevant phases such as the liquid-ordered phase and solid-lipid interfaces with biologically functional importance. (hindawi.com)
the gas mixtures subjected to separation are an oxygen/nitrogen system and a carbon monoxide/nitrogen system. (osti.gov)
Longitudinal Relaxation (T 1) of Methane/Hydrogen Mixtures for Operando Characterization of Gas-Phase Reactions. (uni-bremen.de)

Recently, a model for heterochromatin organization based on distinct liquid-like phase has been proposed. (emerging-researchers.org)

It is these waste mobile phase solvents that offer a significant target for green chemistry and engineering improvements. (ddw-online.com)

Unravelling this information could help in engineering more robust membranes in other organisms and in synthetic cells. (basyc.nl)
Glycosphingolipids (GSLs) are a subclass of glycolipids that are found in cell membranes of various organisms ranging from bacteria to humans. (springer.com)

Not much is known about the lipid biosynthesis of tetraether membranes which confer extremophilic characteristics to archaea. (basyc.nl)

Despite its prominence and importance, our understanding of how the nucleus and its DNA are organized in space and time-and the implications of that organization for proper function-has lagged behind that of other cellular structures. (cshlpress.com)

Operando characterization of heterogeneously catalyzed gas-and multi-phase reactions using nuclear magnetic resonance imaging. (uni-bremen.de)

The TCR signalling cascade is initiated by phosphorylation of critical tyrosines of the TCR/CD3 complex by the Src-family kinases Lck or Fyn which are anchored in the cytoplasmic leaflet of the T cell plasma membrane. (biomedcentral.com)

Tran-Cong-Miyata and co-workers using periodic irradiation in photoreactive polymer blends to control morphology, specifically width of the resultant spinodal modes in the phase separated morphology. (wikipedia.org)
The process is commonly used in control of the morphology of polymer blends, for applications in thermoelectrics, solid-state lighting, polymer electrolytes, composites, membrane formation, and surface pattern formations. (wikipedia.org)
In addition, both the pristine polymer and the dispersion exhibited an interfacial Maxwell-Wagner relaxation, likely due to spatial heterogeneities associated with phase disproportionation in this polymer. (ibecbarcelona.eu)
In the case of two-component polymer solutions, phase separation is induced within the cell size space even under conditions that maintain a uniform phase in bulk. (uni-muenchen.de)

Here, we found that SARS-CoV-2 infection causes marginal activation of signaling sensor IRE1α leading to its phosphorylation, clustering in the form of dense ER-membrane rearrangements with embedded membrane openings, and XBP1 splicing. (bvsalud.org)

For water-insoluble materials such as fat or other tissues, the most common separation procedure is extraction with an organic solvent such as diethyl ether (Zlatkis and Kim 1976). (cdc.gov)
Opportunities for reducing solvent consumption and waste generation in separation sciences used to support industrial pharmaceutical discovery and development are presented and discussed in this article. (ddw-online.com)
Preparative HSCCC with a two-phase solvent system composed of n-hexane-ethyl acetate-n-butanol-water (1:1:8:10, v/v/v/v) was successfully performed by increasing the flow-rate of the mobile phase stepwise from 1.0 to 2.0 ml min−1 after 4 h. (webmedcentral.com)

Within this pathway, Dishevelled interprets and propagates signals from 19 Wnts engaging 10 Frizzled (Fzd) receptors/co-receptors embedded in the plasma membrane (PM). For years, interest in how Dishevelled receives, interprets, and relays this information has been immense. (rupress.org)

The state of non-equilibrium can be driven by flow, mixture, phase transitions, chemical reactions, electrical current, or heat, to name some of the driving factors. (balzan.org)

If phase separation could happen in a bottle at dinner, why not within a cell? (princeton.edu)
Recently, structural MS methods have been shown to be promising tools to achieve this endeavour, including XL-MS and in-cell FPOP, yielding unprecedented insights into the structure and organisation of cellular macromolecular assemblies. (leeds.ac.uk)
The cytosolic ZAP-70 tyrosine kinase is recruited to the T cell plasma membrane via binding to the tyrosine phosphorylated TCR-complex [ 3 ]. (biomedcentral.com)
However specific lipid-mediated interactions at the T cell plasma membrane bilayer are also a central functional element in early TCR signalling. (biomedcentral.com)
As cell membranes are both dynamic and compositionally complex, replicating these aspects in a model membrane are essential. (lu.se)

We examine assembly and adsorption in reverse micellar systems but also driven self-organization of colloidal assemblies in apolar media. (aalto.fi)

To identify chemical agents with pro- or antiviral effects we conducted arrayed high-content image-based multi-cycle infection screens of 1,280 mainly FDA-approved compounds with three human viruses, rhinovirus (RV), influenza A virus (IAV), and herpes simplex virus (HSV) differing in genome organization, composition, presence of an envelope, and tropism. (bvsalud.org)

Li and co-workers employed holography, a process of holographic polymerization, in to order to direct the phase separated structure to have the same patterns as the holographic field. (wikipedia.org)

Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells. (mpg.de)

The morphology of the final phase separated structures are generally random owing to the stochastic nature of the onset and process of phase separation. (wikipedia.org)
Recently, Hosein and co-workers demonstrated that nonlinear optical pattern formations that occur in photopolymer systems may be used to direct the organization of blends to have the same morphology as the light pattern. (wikipedia.org)

Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Biology covers recent advances in our understanding of nuclear organization and function. (cshlpress.com)
Our group has also found that heterochromatin contributes to the stiffness of the nuclear membrane. (emerging-researchers.org)

In most cases, the condensed phase is not in equilibrium with the vapor phase, and as a consequence there will be evaporation of water and possible also other volatile components. (lu.se)
Similar conditions also exist in living system, with obvious examples found in the lipid tear film formed on our eyes, which prevent evaporation and dry eyes, and the membrane system in the alveoli of the lung. (lu.se)

The organic analytes partition between the water sample and the stationary phase coating of a fused silica fiber before they are thermally desorbed in a GC injector. (cdc.gov)

At the interface between two regions, for example the air-liquid interface of a lipid solution, there can arise non-equilibrium situations. (lu.se)

In particular, the water selective permeation membrane for a mixture of water and alcohol has already arrived at the stage of bench plant demonstration. (osti.gov)

This subcomplex also exists in the peroxisome membrane. (tcdb.org)

Separation and Purification Technology, 300, 121792. (uni-bremen.de)

2018 ). Pex5p and Pex7p have been shown to traverse the membrane ( Kerssen et al . (tcdb.org)

As a result of this self-organization, cells drive fluid flows that circulate around the colony at a constant peak speed of ~30 µm s −1 , providing a stable and high-speed avenue for directed material transport at the macroscopic scale. (nature.com)

Self-Assembly of Solid-Supported Membranes Using a Triggered Fusion of Phospholipid-Enriched Proteoliposomes Prepared from the Inner Mitochondrial Membrane. (quantabiodesign.com)

Anatomy43

Organisms12

Diseases1

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Psychiatry and Psychology2

Phenomena and Processes63

Disciplines and Occupations6

Technology, Industry, Agriculture9

Information Science5

Health Care10

Proteins27

Lipid membranes2

Protein14

Dynamics5

LLPS6

Lipids3

Plasma membranes2

Compartments6

Undergoes liquid-liquid phase separation1

Fluorescence microscopy2

Actin1

Multicomponent4

Receptor5

Polymers2

Chromatography13

Biological4

Molecular5

20171

Emerge2

Rafts5

Vesicles2

Lateral5

Raft2

Intracellular1

Molecules3

20231

Mixtures4

Distinct1

SOLVENTS1

Organisms2

Characteristics1

Nucleus1

Characterization1

Leaflet1

Polymer4

Phosphorylation1

Solvent3

Receptors1

Transitions1

Cell5

Assemblies1

Genome1

Patterns1

Interaction1

Morphology2

Nuclear2

Evaporation2

Organic1

Regions1

Selective1

Subcomplex1

Purification1

20181

Flows1

Self-Assembly1