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  • absence
  • How the replication machinery that forms this inclusion remains associated in the absence of a membrane has been an enduring mystery. (asm.org)
  • 3,14-21 The liquid-like nature and absence of an encapsulating membrane give rise to a direct link with processes taking place in the cytoplasm or nucleoplasm, 3 providing for functions such as sequestration, buffering and reaction rate modulation. (rsc.org)
  • In the absence of DPPC and water, the compounds showed thermotropic liquid-crystalline behavior with a transition between polyphilic and amphiphilic assembly (see part 1 in this issue). (mdpi.com)
  • cell
  • Organization at the shortest and longest ends of these scales is increasingly well understood through work in structural biology/biophysics and cell biology, respectively. (utsouthwestern.edu)
  • Hydrophobic
  • Most of these proteins contain a C-terminal putative transmembrane (TM) fragment and a pair of hydrophobic alpha helices (alpha5-alpha6) similar to the membrane insertion fragments of the ion-channel domain of diphtheria toxin and colicins. (jove.com)
  • cellular organization
  • Our work is directed toward both understanding the structure and dynamics of individual proteins and their signaling complexes, as well as discovering how and why these discrete entities produce cellular organization and activities at longer length and time scales. (utsouthwestern.edu)
  • The study's senior author, Amy S. Gladfelter of University of North Carolina, Chapel Hill, began this work as part of the HHMI / HCIA Summer Institute at the MBL, a group of 70 scientists who explored this emerging paradigm of cellular organization over five summers of intense, synergistic research. (uncommondescent.com)
  • synthesis
  • Nonsegmented negative-strand (NNS) RNA viruses, which include some of the most significant human, animal, and plant pathogens extant, form inclusions that are sites of RNA synthesis and are not circumscribed by a membrane. (asm.org)
  • cells
  • Expression of the individual viral components of the replication machinery in cells demonstrates that the 3 viral proteins required for replication are sufficient to drive cytoplasmic phase separation. (asm.org)
  • 1,2 Besides membrane-encapsulated organelles, cells are known to form different types of membraneless organelles with varying compositions. (rsc.org)
  • domains
  • We record coexisting liquid domains over a range of composition and temperature significantly wider than previously reported. (jove.com)
  • structures
  • Phase separation is increasingly recognized as responsible for cellular structures as diverse as processing bodies (P-bodies) and nucleoli and was recently demonstrated for rabies virus. (asm.org)
  • diseases
  • Thus, understanding cytoplasmic organization may help us find a cure for these diseases and it may bring us closer to solving the enigma of aging. (mpi-cbg.de)
  • structure
  • In DPPC membranes, various trends in the domain morphologies were observed upon structure variations, which entailed branched alkyl chains of various sizes, alkyl chain semiperfluorination and size expansion of the headgroups. (mdpi.com)
  • MRNA structure determines specificity of a polyQ-driven phase separation. (uncommondescent.com)
  • complex
  • Implementing complex reaction networks in a biomimetic compartment requires not only a detailed understanding of the reactions involved, but also control over their spatial organization. (rsc.org)