Organism metabolic engineering. Medical search. Frequent questions

To quickly elucidate the metabolism of this non-model organism, several parallel 13 C-fingerprinting experiments were designed to probe multiple pathways in Rhodococcus opacus PD630. (confex.com)
Metabolic engineers tinker with the series of reactions that the host organisms use to build new molecules, adding genes for new enzymes that reshape these natural synthetic pathways. (mit.edu)
On the other hand, the complexity of plant synthetic pathways means that in many cases, it may be easier to modify the plant rather than try to reconstitute the entire plant pathway in a different organism such as bacteria. (mit.edu)
This is achieved by rational engineering of the endogenous metabolic genes and pathways using genetic switches and circuits as well as transplanting genes from heterologous sources by using recombinant DNA methods. (ibab.ac.in)
Using gene editing technologies we also seek to modify metabolic pathways in yeast cells to generate "humanised yeast stains" for production of human therapeutic proteins and biologics. (ibab.ac.in)
A Unique Bioengineering Platform for the Efficient Optimization of Metabolic Pathways- Kedar Patel, DNA2.0 Inc. (aiche.org)
Steady-state and dynamic isotope tracer experiments can provide information on metabolic network structure, metabolic fluxes, and thermodynamics of metabolic reactions and pathways. (energy.gov)
Life Technologies offers a comprehensive portfolio of tools and services designed for metabolic engineering, enabling a more effective approach to optimizing organisms and bioproduction pathways, empowering the development of biofuels, energy crops, biobased chemicals, and CO 2 sequestration methods. (thermofisher.com)
and metabolic flux analysis, and protein signaling pathways and engineering. (washington.edu)
The authors describe, in depth, the biosynthetic pathways, physical properties and industrial production processes and discuss in detail the genetic and metabolic engineering of microorganisms for biopolymer production. (caister.com)
Scientists and Engineers at Conagen use the latest synthetic biology tools to design metabolic pathways, improve production organisms, optimize production procedures, and ensure the quality as well as safety of the final products. (massbio.org)
We are passionate about understanding and engineering metabolic pathways. (massbio.org)
The essential biochemistry, genetics, cell biology, and metabolic pathways underlying the survival and success of all living organisms. (queensu.ca)
However, the promoters used in most pathway engineering studies in S. cerevisiae have not been characterized and compared in parallel under multiple conditions that are routinely operated in laboratory and the number of known promoters is rather limited for the construction of large biochemical pathways. (nih.gov)
Compared to non-living systems, living organisms have more complex biochemical processes, which can be comprehended by studying the most important metabolic pathways and their control mechanisms. (lu.se)
Additionally, specific genes can be inserted into the virus that then can repair defective metabolic, enzymatic, or synthetic pathways in the host. (medscape.com)

This work provides a strategy to improve carbon fixation efficiency in photosynthetic organisms. (osti.gov)

We then implement the pathway in a photosynthetic organism Synechococcus elongates PCC7942, and show that it increases the intracellular acetyl-CoA pool and enhances bicarbonate assimilation by roughly 2-fold. (osti.gov)
Ethanol pathway engineering. (researchgate.net)
This session emphasizes pathway-level engineering. (confex.com)
Example areas include: strategies to enhance the production of natural products, pathway engineering for the production of novel products, and the application of evolutionary techniques for improving production of a metabolite. (confex.com)
Metabolic engineering rarely succeeds at the first attempt to introduce and activate a heterologous pathway in a host organism. (biomedcentral.com)
Our analysis of metabolic regulation shows that UV activates DNA repair, aromatic amino acid and carotenoid biosynthesis and represses central carbon metabolism and the fungal-like apoptotic pathway. (biorxiv.org)
These gaps in sequence data significantly limit the accuracy of genome annotation and metabolic pathway prediction, and are a barrier for metabolic engineering. (sri.com)
Corynebacterium glutamicum has recently been engineered to produce biofuel additives 2-methyl-1-butanol and 3-methyl-1-butanol isomers via the Ehrlich pathway to accumulate 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. (pharmiweb.com)
As an example of application for these promoters in metabolic engineering, the genes involved in xylan degradation and zeaxanthin biosynthesis were subsequently cloned under the control of promoters with medium to high strength and assembled into a single pathway. (nih.gov)

Engineering new genes into plants has been done before: In the 1990s, scientists developed corn that could produce an insecticide called Bt, which comes from a bacterial gene. (mit.edu)
Most metabolic engineers use bacteria as their host organism, in part because their genes are easier to manipulate. (mit.edu)
This is a very nice study that might get more people to think about engineering plants rather than using them as a source of genes," says Eisenstein, who was not involved with the research. (mit.edu)
In the new study, they engineered the cells to express genes that code for enzymes that attach chlorine or bromine to vinblastine precursors and other alkaloids. (mit.edu)
However, writing such summaries is a daunting task, given the number of genes in each organism (e.g. 13,929 protein coding genes in Drosophila melanogaster). (stanford.edu)
CSE 488: Laboratory Methods In Synthetic Biology Designs and builds transgenic bacterial using promoters and genes taken from a variety of organisms. (washington.edu)
New metabolic engineering approaches have recently been developed to modulate carotenoid content, including the employment of CRISPR technologies for single-base editing and the integration of exogenous genes into specific "safe harbors" in the genome. (frontiersin.org)
The virus itself can be engineered to remove its replicative capacity by removing essential genes. (medscape.com)

Metabolic modeling, which helps making sense of the metabolism in a biological network, is an important tool for engineering biocatalysts, with applications in biofuels, drug design, microbial community analysis, and personalized medicine. (nature.com)
In addition, provided herein are conjugate vaccine carriers comprising engineered tetanus toxin variants and methods of using such conjugate vaccines to elicit T-cell dependent immune memory responses which can target a broad spectrum of microbial pathogens as a single vaccine. (justia.com)
Abstracts describing new developments in these topics are sought, and the session is open to papers focused on any type of organism, microbial consortium, or cell-free system. (confex.com)
Understanding of metabolism is also of central importance to the microbial production of drugs, bioproducts and biofuels through metabolic engineering [ 5 ]. (biomedcentral.com)
3. Microbial preparations produced from natural or artificially-modified microbe organisms or their metabolic products, and used to control air pollution, water pollution, or soil pollution, treat waste, or control environmental sanitation disease vectors. (gov.tw)
This work will aid in the construction of accurate metabolic models that incorporate thermodynamic constraints and guide fast rational engineering of microbial networks. (energy.gov)
The editor and the authors have produced an excellent up-to date compendium which is extremely useful for all students of biotechnology, engineering and scientists in the biotechnological and microbiological branches and is recommended for all biotechnological and microbial laboratories and enterprises in this field. (caister.com)
Genomics has become the primary way to explore microbial diversity, because genetic tools are currently difficult to develop in non-model organisms. (biorxiv.org)
Because lipids are present in all living organisms, other areas of applications such as plant, microbial and nutritional research could also benefit from improvements in lipid analysis and a better understanding of lipid metabolism. (chromatographyonline.com)

By applying engineering principles to biological research we have the potential to shape the future of industrial compounds, biofuels and new medicines, and to provide exciting insights into fundamental biological mechanisms," BBSRC Chief Executive Douglas Kell said in a statement from the council. (genomeweb.com)
jQMM will facilitate the design and metabolic engineering of organisms for biofuels and other chemicals, as well as investigations of cellular metabolism and leveraging -omics data. (biomedcentral.com)
Corynebacterium glutamicum is known for its role in the industrial production of L-glutamate and L-lysine and as a platform organism for the production of a variety of fine chemicals, biofuels and polymers. (pharmiweb.com)

In between these two levels is the proteome that performs the biochemical transformations according to the genetic template, though it is this middle step in the process that cannot yet be robustly and efficiently incorporated into models of metabolic systems. (nature.com)
Some areas of biotechnology include genetic engineering, metabolic engineering, personalized medicine, drug development, applied microbiology and fermentation techniques, and biological control of insect pests. (schoolfinder.com)
The yeast Saccharomyces cerevisiae is a favored industrial organism due to its robustness, low nutritional requirements, high tolerance to low pH, suitability for large-scale fermentation, and ease of genetic manipulation. (aiche.org)
The curriculum explores the rapidly expanding field of genetic engineering and the nearly unlimited potential that controlled genetic experiments hold for improving the quality of life. (rit.edu)
The disclosure discloses cathelicidin-expressing lactic acid bacteria, and belongs to the technical field of genetic engineering. (justia.com)
Through development of genetic tools, support of a metabolic model, and characterization of different growth modes such as biofilm formation, we seek to improve the prospects for cyanobacteria as industrial production platforms. (ucsd.edu)
Explore our solutions for De novo and transcriptome sequencing, plant genotyping, QTL mapping, plant genetic engineering and more here. (thermofisher.com)
The genetic manipulation of microorganisms opens up an enormous potential for the biotechnological production of biopolymers with tailored properties suitable for high-value medical application such as tissue engineering and drug delivery. (caister.com)
Here, we demonstrate that -omics can be leveraged to accelerate genetic tool development for the basidiomycete yeast Xanthophyllomyces dendrorhous CBS 6938, the sole biotechnologically relevant organism in the Tremellomycete family. (biorxiv.org)
Thus, -omics-to-parts workflows can simultaneously provide useful genomic data and advance genetic tools for non-model microbes, particularly those without a closely related model organism. (biorxiv.org)
Themes and case studies could range from the application of genetic engineering in biotechnology to the role of cellular dysregulation in inheritable diseases. (queensu.ca)
Genetic engineering of carotenogenesis made possible the enhancement of the nutritional value of many crops. (frontiersin.org)

This method is a qualitative alternative to 13 C-Metabolic Flux Analysis that significantly reduces the time and expense for analyzing cell metabolisms. (confex.com)
Firstly, it presents a complete toolbox for simultaneously performing two different types of flux analysis that are typically disjoint: Flux Balance Analysis and 13 C Metabolic Flux Analysis. (biomedcentral.com)
Moreover, it introduces the capability to use 13 C labeling experimental data to constrain comprehensive genome-scale models through a technique called two-scale 13 C Metabolic Flux Analysis (2S- 13 C MFA). (biomedcentral.com)
One salient example of this is metabolic flux analysis in industrially relevant cellulolytic microbes, such as Clostridium thermocellum , that metabolize complex substrates (i.e., lignocellulosic biomass). (energy.gov)

Metabolic engineering is the targeted improvement of the cellular metabolic phenotype using recombinant DNA technology. (confex.com)

FAST, a small 14 kDa protein, was engineered from the photoactive yellow protein (PYP) by directed evolution. (wikipedia.org)
However, O'Connor's approach, known as metabolic engineering, goes beyond simply adding a gene that codes for a novel protein. (mit.edu)
Electrical engineers also have determined methods to analyze circuits, which exist inside cells in the form of webs of protein interactions. (mit.edu)
I have expertise from gene characterization to protein engineering. (queensu.ca)
While the functions of protection and thermoregulation are well recognized, skin also has important metabolic functions in protein and vitamin D metabolism. (medscape.com)

As a scientist, I have 30 years of experience conducting research on various molecular problems using a range of organisms, from bacteria to plants. (queensu.ca)
For example, a diet rich in dietary carbohydrate such as refined sugar favors bacteria such as Streptococcus mutans , the organism that causes dental caries. (medscape.com)

Saccharomyces cerevisiae is an important platform organism for synthesis of chemicals and fuels. (nih.gov)

Yeast is recognized as GRAS organism and used extensively for industrial production of valuable molecules. (ibab.ac.in)
Because cyanobacteria grow photosynthetically using water and CO 2 and are easy to manipulate genetically, they are attractive organisms for the production of molecules that have industrial applications. (ucsd.edu)
regulation of antibiotic production through signalling molecules and noncoding RNAs in Streptomyces coelicolor , the model organism of the most important group of industrial antibiotics producers. (manchester.ac.uk)
We engineer live organisms to produce molecules that are essential to our well-beings. (massbio.org)
Most of the metabolic engineering studies on these yeast species focused on other molecules compounds than lipids. (hal.science)

Integrate advanced mass spectrometry, computational modeling, and metabolic engineering to develop an experimental-computational approach for the in vivo genome-scale determination of Gibbs free energies ( ΔG ) in metabolic networks suitable for high-throughput thermodynamic profiling of engineered organisms and emerging model systems. (energy.gov)

In metabolic engineering, strains are modified and controlled at the genome level through the transcriptome, and the effects are observed at the fluxome level, which accounts for the range of metabolic reactions in an organism. (nature.com)
We are generating value-added yeast strains that overexpress phytochemicals, food supplements, human metabolic enzymes and nutraceuticals. (ibab.ac.in)
Therefore, yeast strains engineered to produce other products are suboptimal due to their production of ethanol as a major byproduct that significantly diminishes the yield of the desired product. (aiche.org)
Strains have been developed to overcome this central challenge in yeast metabolic engineering by deleting either pyruvate decarboxylases, or alcohol dehydrogenases involved in ethanol formation, which dramatically reduces ethanol formation. (aiche.org)
The use of engineered strains as a basis for production of acetoin, a widespread food flavor, is proposed. (pharmiweb.com)

We utilize latest genome editing technologies and DNA assembly methods to engineer microorganisms, mosquito and human cells. (ibab.ac.in)
Furthermore, the utilization of engineered microorganisms allowed efficient mass production of carotenoids, making it convenient for industrial practices. (frontiersin.org)

Among different modeling schemes, the quantification of intracellular metabolic fluxes (i.e. the rate of each reaction in cellular metabolism) is of particular interest for metabolic engineering because it describes how carbon and energy flow throughout the cell. (biomedcentral.com)
The jQMM library presented here provides an open-source, Python-based framework for modeling internal metabolic fluxes and leveraging other -omics data for the scientific study of cellular metabolism and bioengineering purposes. (biomedcentral.com)
Metabolism is the set of physical and chemical processes through which living organisms extract energy and mass from their environment in order to sustain life [ 1 ]. (biomedcentral.com)
Most importantly, the very structure of organisms is dependent on the continuous metabolism of nutrients into ATP, and its use in maintaining homeostasis. (lu.se)

Chemical engineers learn how to maximize the products of reactions, a skill that is useful when engineering cellular metabolic processes to produce certain compounds. (mit.edu)
However, studies from the biological sciences indicate that much of the brain's processes are in place to maintain allostasis, both of the brain itself and of the organism as a whole. (lu.se)
We will try to show that this belief disregards the central characteristics of the complex self-organized adaptive evolutionary processes of organisms in general. (bvsalud.org)

Classically, model-based strain design has relied on tools that use the DNA sequence of an organism and homology with well-studied organisms to infer a network of metabolic reactions that happen inside a cell of that organism, which is called a genome-scale model (GEM). (nature.com)

Now MIT chemists have found a new way to expand plants' pharmaceutical repertoire by genetically engineering them to produce unnatural variants of their usual products. (mit.edu)

Sequence and annotate whole genomes of a variety of organisms using the Illumina MiSeq in the Genomics Lab . (rit.edu)

MIT chemists engineer the periwinkle plant to produce compounds that could become more effective cancer drugs. (mit.edu)
MIT associate professor Sarah O'Connor, right, and graduate student Weerawat Runguphan have engineered periwinkle root cells that can produce variations of compounds that the plant normally synthesizes. (mit.edu)
Department of Chemical Engineering, Lund University, derived compounds. (lu.se)

Third, feedstocks must be pretreated so fickle organisms or unit operations downstream won't be corrupted. (biocycle.net)

Abstract: When organisms locomote and interact in nature, they must navigate through complex habitats that vary on many spatial scales, and they are buffeted by turbulent wind or water currents and waves that also vary on a range of spatial and temporal scales. (usc.edu)

and inserting artificial networks into cells and organisms that provide new functions. (genomeweb.com)
Cells of diverse organisms, from cyanobacteria to humans, use a circadian (24 h) clock to control physiological events and gene expression. (ucsd.edu)
Combining rich optical contrast and scalable ultrasonic resolution, photoacoustic imaging is the only modality capable of providing multiscale high-resolution structural, functional, metabolic, and molecular imaging of organelles, cells, tissues, and organs as well as small-animal organisms in vivo. (caltech.edu)

This project will center on applying nonlinear causal inference to identify interaction between scales of complexity in natural systems (e.g. single fish populations and ecosystem functioning or single cell expression and organism physiology). (bu.edu)

However, incorporating expression information into metabolic networks poses a significant challenge, and most current models do not even attempt it-effectively excluding an important network in biological systems that can drastically affect results. (nature.com)
The emergence of biological engineering can be compared to the growth of electrical engineering from physics and chemical engineering from chemistry. (mit.edu)
Synthetic biology, a particular field in biological engineering, uses what is already known in biology to redesign or design from scratch biological systems to carry out useful functions. (mit.edu)
Perhaps engineering concepts in other disciplines can be transferred to work with biological systems. (mit.edu)
NEW YORK (GenomeWeb News) - A European Commission-led synthetic biology program plans to use €15.5 million ($20 million) to fund international, multi-partner projects that will use synthetic biology in a range of research areas, including genomics, biological nanoscience, metabolic engineering, and other areas. (genomeweb.com)

Though humans have long recognized the value of medicinal plants, "there has been a lot less effort to engineer plants to produce things that could be of even more value," says Edward Eisenstein, associate professor at the University of Maryland Biotechnology Institute. (mit.edu)
Biotechnology applies the principles of biochemistry and biology to the study and manipulation of living organisms for industrial, medical, agricultural and environmental applications. (schoolfinder.com)
Eriko Takano is Professor of Synthetic Biology in the Manchester Institute of Biotechnology, Department of Chemistry, School of Natural Sciences, Faculty of Science and Engineering, at the University of Manchester. (manchester.ac.uk)

Traditional biochemical tests and an API20Strep system assay (BioMérieux, Marcy l'Etoile, France) suggested that the organism was S. suis . (cdc.gov)

This optimized strain can be engineered to produce different fuels or chemicals of interest from glucose, with significantly enhanced yields. (aiche.org)
Current uses of synthetic biology include engineering synthetic rubber to be used for tires, generating "green chemicals" from agricultural waste, and creating energy saving fuels. (renewable-carbon.eu)

Mathematical modeling helps provide a mechanistic understanding for the studied process, separating the main drivers from the circumstantial ones, bounding the outcomes of experiments and guiding engineering approaches. (biomedcentral.com)

This work represents one of the first metabolic engineering of B. adeninivorans for lipid production. (hal.science)

Ujor's research deploys metabolic engineering and fermentation as tools for understanding the complex metabolic networks in fermentative organisms. (asm.org)

Because of the complex interplay between these different layers of control, understanding expression and incorporating this into future models is key for improving metabolic engineering. (nature.com)
The EMBL-EBI Complex Portal (www.ebi.ac.uk/intact/complex) is a central service that provides manually curated information on stable, on macromolecular complexes from model organisms. (stanford.edu)

This approach will be broadly useful in current efforts to engineer diverse microbes. (biorxiv.org)

Since synthetic biology is such a new discipline, how does one go about engineering biology? (mit.edu)

Joining AIChE gives you access to an amazing network of top professionals in chemical engineering and related fields. (aiche.org)
I grew up in Albuquerque New Mexico and did my undergraduate in Chemical Engineering at the New Mexico Institute of Mining and Technology. (wrfseattle.org)
This simplifies the model definition greatly and covers systems such as porous membranes, filters, and other common devices in chemical engineering and material science. (comsol.de)

Marcus received his PhD, which focused on the production of cellulose-based scaffolds for tissue engineering, from the University of Bath, UK. (ubc.ca)
We chose oleaginous yeasts species of Blastobotrys genus for defining a new model organism for fatty acid production and lipids, because these oleaginous yeasts natively produce higher lipids rate than Yarrowia lipolytica in the same conditions and can metabolize glucose and xylose. (hal.science)
La plus forte production de lipides vient du cellobiose (35%) et du glucose (32%).Ensuite, afin de mieux comprendre le métabolisme des lipides des levures du genre Blastobotrys, nous avons exploré l'effet de la température sur leur physiologie, production de lipides et le profil lipidique en utilisant un milieu YNB contenant 30 g/L de glucose. (hal.science)
Nous n'avons pas trouvé de différence marquée de transition de formes entre les formes hyphes et les levures en milieu YNB sous l'effet de quatre températures (28°C, 37°C, 42°C, 45°C), mais la production des lipides est favorisée à 28°C et le C18:1 est l'acide gras le plus abondant dans le profil lipidique. (hal.science)
Corynebacterium glutamicum is the most important industrial organism for amino acid production. (pharmiweb.com)

Model accuracy is instrumental to the success of these applications through an efficient engineering of the host organisms. (nature.com)

various species of organisms from the It is important to ascertain the cause surface burn wounds were roughly pro- of the burn because this may be helpful portional to those from blood specimens or in determining burn depth. (who.int)

Provided herein are engineered non-catalytic, non-toxic tetanus toxin variants and methods of using such engineered tetanus toxin variants as low dose, protective vaccines that are non-toxic and more potent than their respective chemically inactivated toxoids. (justia.com)

[ 1 ] In addition to providing a physical barrier to pathogenic organisms, skin functions as an active immune organ with distinctive antigenic properties that play a significant role with particular regard to composite tissue allotransplantation. (medscape.com)

They are widely distributed in plants from different families and their effect stems from the ability to interact with membrane sterols, disrupting the cell integrity from target organisms ( 34 - 37 ). (elifesciences.org)

Field and laboratory measurements enabled us to quantify the fine scale, rapidly changing patterns of water velocity vectors and of chemical cue concentrations near coral reefs and along fouling communities (organisms growing on docks and ships). (usc.edu)

Anatomy2

Organisms26

Chemicals and Drugs65

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes40

Disciplines and Occupations10

Technology, Industry, Agriculture19

Information Science7

Health Care3

Pathways16

Photosynthetic organisms1

Pathway9

Genes8

Microbial9

Biofuels3

Genetic12

Flux Analysis4

Recombinant DNA techno1

Protein5

Bacteria2

Platform organism1

Molecules5

High throughput1

Strains5

Microorganisms2

Metabolism4

Processes3

Genome1

Genetically1

Variety of organisms1

Compounds3

Feedstocks1

Abstract1

Cells3

Physiology1

Biological5

Biotechnology3

Biochemical1

Chemicals2

Approaches1

Lipid Production1

Fermentation1

Complex2

Microbes1

Synthetic Biology1

Chemical Engineering3

Production5

Model1

Important1

Variants1

Barrier1

Cell1

Concentrations1