Organic acids corynebacterium glutamicum. Medical search. Frequent questions

2022. "Cg1246, a New Player in Mycolic Acid Biosynthesis in Corynebacterium Glutamicum. (paris-saclay.fr)
Using E.coli cells as an example, it was shown that the aromatic biosynthesis pathway (shikimic acid pathway) in bacteria could be successfully optimised for the production of aromatic amino acids such as L-phenylalanine, L-tryptophan, p-aminophenylalanine and phenylglycine. (uni-stuttgart.de)
[1] the ionic form is known as glutamate ) is an α- amino acid that is used by almost all living beings in the biosynthesis of protein s. (explained.today)
Ergothioneine production by Corynebacterium glutamicum harboring heterologous biosynthesis pathways. (hokudai.ac.jp)

Corynebacterium glutamicum is the most important industrial organism for amino acid production. (pharmiweb.com)

The manipulation of organisms in order to yield a specific product has many applications to the real world like the production of some antibiotics, vitamins, enzymes, amino acids, solvents, alcohol and daily products. (wikipedia.org)
Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase. (microbiologyresearch.org)
Correlation of organic acids concentration and key enzymes involved in the central carbon metabolism was further determined by enzymatic assays. (biomedcentral.com)
The synthesis of the peptide stem of bacterial peptidoglycan involves four enzymes, the Mur ligases (MurC, D, E and F). Among them, MurD is responsible for the ATP-dependent addition of d -glutamic acid to UDP-MurNAc- l -Ala, a reaction which involves acyl-phosphate and tetrahedral intermediates. (degruyter.com)
For example, in an environment rich in amino acids, cells do not expend energy for making amino acid biosynthetic enzymes. (go.jp)
However, if the environment becomes depleted of amino acids (nutritional downshift), cells will be exposed to a lack of both the amino acid biosynthetic enzymes and the amino acids required to make these enzymes. (go.jp)
Once amino acid biosynthetic enzymes start to accumulate, the cell is able to produce its own amino acids, and a new growth phase begins. (go.jp)
In Escherichia coli , besides the PEPCK reaction, there is another pathway to form PEP from C 4 -dicarboxylic acid which involves the NAD- and NADP-dependent malic enzymes (MAEA and MAEB) and phosphoenolpyruvate synthase (PPS). (asm.org)
Strains deficient in all three enzymes cannot grow on C 4 -dicarboxylic acids as a sole carbon source, whereas strains retaining one of these enzymes are still able to grow ( 14 ). (asm.org)

Corynebacterium pseudotuberculosis is a facultative intracellular pathogen that belongs to the so-called CMN ( Corynebacterium-Mycobacterium-Nocardia ) group, a distinct subgroup of the Actinobacteria that also includes other highly important bacterial pathogens, such as Corynebacterium diphtheriae and Mycobacterium tuberculosis . (biomedcentral.com)

Corynebacterium glutamicum is known for its role in the industrial production of L-glutamate and L-lysine and as a platform organism for the production of a variety of fine chemicals, biofuels and polymers. (pharmiweb.com)
[14] Chemical synthesis was supplanted by the aerobic fermentation of sugars and ammonia in the 1950s, with the organism Corynebacterium glutamicum (also known as Brevibacterium flavum ) being the most widely used for production. (explained.today)

Reyes, A.M. PrxQ B from Mycobacterium tuberculosis is a monomeric, thioredoxin-dependent and highly efficient fatty acid hydroperoxide reductase. (uba.ar)
Trujillo, M. 'PrxQ B from Mycobacterium tuberculosis is a monomeric, thioredoxin-dependent and highly efficient fatty acid hydroperoxide reductase' (2016) Free Radical Biology and Medicine. (uba.ar)

Corynebacterium glutamicum has recently been engineered to produce biofuel additives 2-methyl-1-butanol and 3-methyl-1-butanol isomers via the Ehrlich pathway to accumulate 2-keto-3-methylvalerate and 2-ketoisocaproate, respectively. (pharmiweb.com)
Overall, this study has provided insights on the understanding on the effect of triglycerides and sugar as carbon source in fungi global metabolic pathway, which might become important for future optimization of carbon flux engineering in fungi to improve organic acids production when vegetable oil is applied as the sole carbon source. (biomedcentral.com)
This reaction is the first step in the gluconeogenic pathway, in which citric acid cycle intermediates are converted to hexose. (asm.org)
Conversely, PEPCK also functions in the anaplerotic pathway of some organisms, such as Anaerobiospirillum succiniciproducens ( 37 ), Alcaligenes eutrophus ( 39 ), Ruminococcus flavefaciens ( 40 ), Dipetalonema viteae ( 4 ), and Trypanosoma cruzi ( 6 ), where it forms OAA from PEP, which in turn enters the citric acid cycle. (asm.org)
The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. (brenda-enzymes.info)

analyses gradually elucidated the perfected genome-scale metabolic map of C. glutamicum , expanding the scope of its application. (frontiersin.org)
Metabolic network analysis during fed-batch cultivation of Corynebacterium glutamicum for pantothenic acid production: first quantitative data and analysis of by-product formation. (microbiologyresearch.org)
Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. (microbiologyresearch.org)
We are also working on the targeted modification of the metabolic pathways of E. coli and Corynebacterium glutamicum for the production of aromatic compounds, e.g. amino acids such as phenylalanine or tryptophan, violacein and other fine chemicals. (uni-stuttgart.de)
Metabolic engineering for glycoglycerolipids production in E. coli: tuning phosphatidic acid and UDP-glucose pathways. (iqs.edu)

At pH values between about 2.5 and 4.1, [4] the carboxylic acid closer to the amine generally loses a proton, and the acid becomes the neutral zwitterion − OOC−CH− 2 −COOH. (explained.today)
At even higher pH, the other carboxylic acid group loses its proton and the acid exists almost entirely as the glutamate anion − OOC−CH− 2 −COO − , with a single negative charge overall. (explained.today)

Corynebacterium glutamicum is a traditional food-grade industrial microorganism, in which an efficient endotoxin-free recombinant protein expression factory is under development in recent years. (pharmiweb.com)

Ten peaks were obtained in the C. glutamicum ATCC 14067 genome including two new peaks related to three operons (RS_01910-RS_01915, RS_15995, and RS_16000). (frontiersin.org)
2020. "Genome-Wide Identification of Novel Genes Involved in Corynebacteriales Cell Envelope Biogenesis Using Corynebacterium Glutamicum as a Model. (paris-saclay.fr)

There has been much research on AmtR structure, functions, and regulons in the type strain C. glutamicum ATCC 13032, but little research in other C. glutamicum strains. (frontiersin.org)

CRISPR interference (CRISPRi) technology was applied to repress the competing pathways ( pgi , pck , and pykgenes) in C. glutamicum, resulting in titer enhancement ratios of l-lysine and l-glutamate production comparable to levels achieved by gene deletion. (pharmiweb.com)
Glutamic acid is produced on the largest scale of any amino acid, with an estimated annual production of about 1.5 million tons in 2006. (explained.today)
alpha-ketoisovalerate decarboxylase Kivd from Lactococcus lactis combined with alcohol dehydrogenase Adh3 from Zymomonas mobilis are the optimum candidates for 3-methyl-1-butanol production in Corynebacterium glutamicum. (brenda-enzymes.info)

Fermentation is a reaction where sugar can be converted into a gas, alcohols or acids. (wikipedia.org)

Glucose depletion led to a rapid repression of most glycolytic genes and a corresponding two- to fivefold increased expression of the gluconeogenic genes pps, pck and malE , which are induced by pyruvate, lactate, acetate and/or other organic acids. (microbiologyresearch.org)
In the case of both E. coli and C. glutamicum, it was shown that the targeted chromosomal integration of genes (partly in higher gene copy numbers) has advantages over plasmid-based expression. (uni-stuttgart.de)
Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)

In Escherichia coli , amino acid starvation leads to the accumulation of an unusual molecule, polyphosphate (polyP), a linear polymer of many hundreds of orthophosphate residues. (go.jp)

L-Glutamic acid and its salts are used as flavor enhancers. (pharmiweb.com)
thiooxidans are significantly enriched in hydroxy (serine and threonine) and amide (glutamine and asparagine) residues, and significantly reduced in charged residues (aspartic acid, glutamic acid, arginine and lysine). (bvsalud.org)
When glutamic acid is dissolved in water, the amino group (−) may gain a proton , and/or the carboxyl groups may lose protons, depending on the acidity of the medium. (explained.today)
Although they occur naturally in many foods, the flavor contributions made by glutamic acid and other amino acids were only scientifically identified early in the 20th century. (explained.today)
[10] In 1908, Japanese researcher Kikunae Ikeda of the Tokyo Imperial University identified brown crystals left behind after the evaporation of a large amount of kombu broth as glutamic acid. (explained.today)
He then patented a method of mass-producing a crystalline salt of glutamic acid, monosodium glutamate . (explained.today)

Grisemycin, salinipeptin, and cypemycin belong to the linaridin class of ribosomally synthesized and posttranslationally modified peptides that contain multiple dehydrobutyrine and D-amino acid residues. (hokudai.ac.jp)
Furthermore, we demonstrated that GrmH/GrmL complex accepted salinipeptin and cypemycin precursor peptides, which possess three additional amino acids. (hokudai.ac.jp)

A basic pattern of gene expression and of relative expression levels during different growth phases was obtained for Corynebacterium glutamicum R grown on different carbon sources. (microbiologyresearch.org)
Targeted metabolomics on central carbon metabolism of tricarboxylic acid (TCA) cycle and glyoxylate cycle were analysed using LC-MS/MS-TripleQ and GC-MS, while untargeted metabolite profiling was performed using LC-MS/MS-QTOF followed by multivariate analysis. (biomedcentral.com)
A R. palustris No. 7 PEPCK-deficient strain showed growth characteristics identical to those of the wild-type strain either anaerobically in the light or aerobically in the dark when a C 4 -dicarboxylic acid, such as succinate or malate, was used as a carbon source. (asm.org)
In Rhizobium species, a PEPCK-deficient mutant fails to grow on minimal medium with succinate or other citric acid cycle intermediates but can grow on glucose and glycerol as a sole carbon source, suggesting that PEPCK functions here as a key gluconeogenic enzyme ( 30 ). (asm.org)

It serves as the precursor for the synthesis of the inhibitory gamma-aminobutyric acid (GABA) in GABAergic neurons. (explained.today)

2020. "The C-Terminal Domain of Corynebacterium Glutamicum Mycoloyltransferase A Is Composed of Five Repeated Motifs Involved in Cell Wall Binding and Stability. (paris-saclay.fr)

The RS_01910, RS_01915, RS_15995, and RS_16000 are not present in the type strain C. glutamicum ATCC 13032. (frontiersin.org)
The research on transcription factors in C. glutamicum was mainly focused on the type strain C. glutamicum ATCC 13032. (frontiersin.org)
By type, the global corynebacterium glutamicum technology market is segmented into S strain and R strain. (pharmiweb.com)

Recently on Mar 07, 2018, a novel L-glutamate exporter of C. glutamicum (MscCG2) was discovered and characterized by Laboratory of Systems & Synthetic Biotechnology led by Prof. SUN Jibin and Prof. ZHENG Ping at Tianjin Institute of Industrial Biotechnology (TIB), Chinese Academy of Sciences. (pharmiweb.com)

To solve this dilemma, cells must use their own proteins as sources of amino acids in response to the nutritional downshift. (go.jp)

Dominique Mengin-Lecreulx was trained in biochemistry, enzymology, molecular biology and organic chemistry and obtained his PhD degree at the University Paris-Sud in 1987. (degruyter.com)

Significant differences in organic acids concentration of about 4- to 8-fold were observed for citric acid, succinic acid, malic acid, and oxaloacetic acid. (biomedcentral.com)

citation needed] Another steroid used is testosterone, which was produced from dehydroepiandrosterone by using the Corynebacterium species. (wikipedia.org)

L-Amino acids find various applications in biotechnology. (pharmiweb.com)

Organic & Biomolecular Chemistry , January. (paris-saclay.fr)
He was trained in organic and medicinal chemistry, enzymology and X-ray crystallography. (degruyter.com)
He was trained in organic chemistry, biochemistry and enzymology, and received his PhD degree in natural sciences from the University Paris-Sud in 1979. (degruyter.com)

Purple nonsulfur bacteria (PNSB) are metabolically versatile organisms which are able to grow either aerobically in the dark or anaerobically in the light using hydrogen or organic compounds as electron donors and subsequently fixing CO 2 ( 16 ). (asm.org)

Binding of common organic UV-filters to the thyroid hormone transport protein transthyretin using in vitro and in silico studies: Potential implications in health. (iqs.edu)

In the genus Corynebacterium , AmtR is a key component of the nitrogen regulatory system, and it belongs to the TetR family of transcription regulators. (frontiersin.org)
Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)

However, in the solid state and mildly acid ic water solutions, the molecule assumes an electrically neutral zwitterion structure − OOC−CH− 2 −COOH. (explained.today)

It is a colorless organic compound that is produced by animals, plants and bacteria. (springeropen.com)

The objectives were to evaluate the effects of Corynebacterium glutamicum cell mass (CGCM) supplementation, replacing blood plasma in diets, on the gene expressions of biomarkers associated with peptidoglycan and their correlation with intestinal health in nursery pigs. (usda.gov)

This study investigated the movements of moisture and acid in skim milk clots formed during dynamic gastric digestion and the effects of milk type (regular or calcium-rich) and the presence/absence of pepsin. (bvsalud.org)

In this study, chromatin immunoprecipitation and massively parallel DNA sequencing (ChIP-seq) was performed to identify the AmtR regulon in C. glutamicum ATCC 14067. (frontiersin.org)
2019. "First Access to a Mycolic Acid-Based Bioorthogonal Reporter for the Study of the Mycomembrane and Mycoloyltransferases in Corynebacteria. (paris-saclay.fr)

Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium Corynebacterium pseudotuberculosis , the etiological agent of caseous lymphadenitis (CLA) in sheep and goats. (biomedcentral.com)

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