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  • vivo
  • We tested the effects of activated NRas(Q61K) on the proliferation, motility, and invasiveness of melanoblasts and melanocytes in the developing mouse and ex vivo explant culture as well as in a melanoma transplant model. (nih.gov)
  • Left anterior descending coronary arteries (LAD) were harvested, cut into cylindric 5 mm segments, and cultured as ex vivo porcine organ cultures (POCs). (nih.gov)
  • Micrographs of sections of porcine coronoary organ cultures at day 28, staining with elastica van gieson: untreated control (A), ex vivo ballooning (B) and ex vivo ballooning with simultaneous intra/extravascular antiproliferative drug therapy (C). arrow = area of neointimal thickening. (nih.gov)
  • Protocol
  • In the attempt to avoid these disadvantages, transepithelial CXL (TE-CXL) technique was introduced, with a protocol based on a specially formulated riboflavin solution, Ricrolin TE (Sooft, Montegiorgio, FM, Italy), featuring two enhancers, trometamol and sodium EDTA, in order to improve stromal penetration. (hindawi.com)
  • advantages
  • This chapter, however, is limited to just one example, i.e., limb bud, that may suffice to bring out feasibility, advantages, and shortcomings of organ culture system in general for short-term teratologic screening purposes. (springer.com)
  • studies
  • Preliminary studies show a transient, early (48 hours) induction of trabecular cell division in response to LTP treatment i human cornealscleral explant organ culture. (elsevier.com)
  • Cell
  • Cell death pattern of a varicose vein organ culture model. (ox.ac.uk)
  • The study aimed to investigate the viability of a varicose vein (VV) organ culture model by assessing cell death pattern. (ox.ac.uk)
  • To assess pattern of cell death with time, VV organ cultures were incubated for up to 14 days with regular medium changed. (ox.ac.uk)
  • To assess viability, cell death of VV organ cultures treated with sodium azide and their untreated counterparts was assayed. (ox.ac.uk)
  • Increased cell death was measured in VV organ cultures from day 0 to 2. (ox.ac.uk)
  • Cell death decreased gradually after day 2 and plateaued from day 8 to 14.VV organ cultures treated with sodium azide demonstrated significantly more cell death in tissue (P = 0.001). (ox.ac.uk)
  • Cell death measured in cultures treated with sodium azide continued to increase until day 7. (ox.ac.uk)
  • In conclusion, this study demonstrated the viability of a VV organ culture model with most cell death occurred within the first two days and then declined to a relatively low level. (ox.ac.uk)
  • Cold Spring Harbor Protocols features organ and cell cultu. (bio-medicine.org)
  • Development
  • 1976). Any of these or other developing organs are suitable subjects for the development of a teratologic screening system. (springer.com)
  • study
  • A preliminary organ culture study was carried out during a sabbatical at the Instituto Superiore di Sanita in Rome in collaboration with Drs. Rita Levi-Montalcini and Domenica Attardi, and the study subsequently was continued at Vanderbilt University. (docme.ru)
  • human
  • Stress-strain in human corneas did not show significant differences among techniques, possibly because of the small sample size of groups. (hindawi.com)
  • systems
  • This work is revealing that the control of body and organ size does not reside in any specific cellular or molecular mechanism but that it is a systems property in which cellular, physiological and environmental signals all contribute in inextricable ways to produce the final phenotype. (duke.edu)