• It remains to be shown whether further improvements in storage methology also can bring other fields of regenerative medicine forward by improving the availability and safety of transplantation of cultured cells. (uio.no)
  • Factors such as increasing approval from the government bodies, increasing awareness regarding the 3D cell structure, augmenting demand for organ transplantation, the rising prevalence of cancer, and widespread application of 3D cell structure in the pharmaceutical and biotech sector are stimulating the market growth to a large extent. (medgadget.com)
  • The 3D cell culture market has been identified as one of the fastest growing markets due to the growing prevalence of cancer across the globe coupled with the growing demand for organ transplantation due to organ failure. (medgadget.com)
  • The labs in Section 2 focus on stem cell transplantation and meiotic staging of germ cells, follicular culture and in vitro techniques related to maturation of oocytes, sperm physiology, fertilization of various animal models, intracytoplasmic sperm injection, and other micromanipulation procedures. (mbl.edu)
  • A tympanal organ is a hearing organ in insects, consisting of a membrane (tympanum) stretched across a frame backed by an air sac and associated sensory neurons. (wikipedia.org)
  • TGF-beta3 maintains the phenotype of disc cells in organ culture. (nih.gov)
  • This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (hindawi.com)
  • This work is revealing that the control of body and organ size does not reside in any specific cellular or molecular mechanism but that it is a systems property in which cellular, physiological and environmental signals all contribute in inextricable ways to produce the final phenotype. (duke.edu)
  • This will include elements such as system priming and cell seeding, the development of optimised and automated cell culture protocols, evaluation of barrier tightness and characterisation of genotype and phenotype of the cultured cells and relate this to their culture environment. (uu.se)
  • It is meritorious for the candidate to show documentation on culture optimisation, immunofluorescent staining, genotypic characterisation, phenotype evaluation and studies of cell growth and proliferation, especially focused on blood-brain barrier specific cells. (uu.se)
  • Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. (google.com)
  • The medium with agar provides the mechanical support for organ culture. (wikipedia.org)
  • For example, in the event of a bacteriological suspicion, specimens from the lesions and internal organs of the fish are inoculated onto agar. (wur.nl)
  • Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for investigating the behavior of animal cells in an isolated and highly controlled environment. (fsu.edu)
  • An improved method of renal tissue engineering, by combining renal dissociation and reaggregation with a low-volume culture technique, results in development of engineered kidneys complete with loops of Henle. (nc3rs.org.uk)
  • Scleroderma refers to a spectrum of disorders characterized by diffuse fibrosis of the skin, blood vessels, synovia, and vital organs, such as the kidneys. (brainscape.com)
  • The bactericidal activity of contaminated standard organ culture medium containing 100 IU/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 μg/ml amphotericin B was evaluated after 48 hours of incubation at 31°C with five inocula of 14 bacteria. (bmj.com)
  • Ellman's Reagent or the GSH-400 assay were used to assay glutathione levels in lenses cultured for different times and under a variety of altered culture conditions. (elsevier.com)
  • Moreover, the emergence of kits, 3D optimized assays, and protocols, coupled with the development of several assay techniques is likely to influence the market growth. (medgadget.com)
  • He has over 25 years experience in cell biology with specific interests in cell culture technology, tissue engineering and stem cell research. (smi-online.co.uk)
  • You are also expected to be a good teacher, as most people in the research team do not have a background in biology and so it is critical that the person filling this position will be able to support team members in several on-going research projects and collaborations on the topic of on-chip cell cultures. (uu.se)
  • A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. (google.com)
  • To define the role of GLUT1 in vascular biology, we established cultured vascular smooth muscle cells (VSMCs) with constitutive upregulation of GLUT1, which led to a threefold increase in glucose uptake as well as significant increases in both nonoxidative and oxidative glucose metabolism as assessed by 13 C-nuclear magnetic resonance spectroscopy. (diabetesjournals.org)
  • For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (labx.com)
  • Inhalation exposure studies of mice were conducted to determine if multi-walled carbon nanotubes (MWCNT) distribute to the parietal pleura, respiratory musculature and systemic organs. (cdc.gov)
  • Through cryopreserving testicular tissue and using an organ culture system that triggered spermatogenesis, the team was able to produce eight healthy baby mice. (medicalnewstoday.com)
  • In past research, the team created an organ culture system that triggered complete spermatogenesis - the process in which the testicles produce sperm - in mice. (medicalnewstoday.com)
  • Using an assisted reproduction technique called "microinsemination" - a method whereby sperm is delivered directly into immature egg cells - the team was able to produce eight healthy baby mice. (medicalnewstoday.com)
  • While cultures are predominantly neuronal, they also contain glia and stem progenitor cells. (zfin.org)
  • We have applied a recently reported technique of embryo explant culture to the analysis of neuronal development and migration in the zebrafish hindbrain. (zfin.org)
  • These results suggest that hindbrain explant culture can be employed effectively in zebrafish to analyze neuronal migration and other dynamic processes using pharmacological and imaging techniques. (zfin.org)
  • We will assess the fates available to each line in an organotypic context and will assess their ability to produce renal structures, up to a whole kidney, in novel tissue engineering culture systems based on our current state-of-the-art. (nc3rs.org.uk)
  • Thus, in the present study, possible involvement of cSox2 and cSox21 in the organogenesis of the digestive organs was demonstrated. (nii.ac.jp)
  • 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. (bu.edu)
  • The bovine pulmonary artery endothelial ( BPAE ) cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 488 conjugated to phalloidin (green fluorescence), and for DNA with the bis-benzimidazole dye Hoechst 33258 (blue fluorescence). (fsu.edu)
  • In this paper, we present a tissue culture, which can among others be used routinely to answer specific questions emerging in the field of photodynamic therapy and fluorescence photodetection. (spiedigitallibrary.org)
  • One reason for such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs and their response to drug compounds. (nature.com)
  • Few engineered model systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far reflected the interactive nature of the human body. (nature.com)
  • In the EMBLA research group, led by Dr. Maria Tenje, one of our research foci is development of microfluidic systems for organ-on-chip applications. (uu.se)
  • To strengthen the team, we are now looking to recruit a postdoc with a biological/medical background to focus on cell cultures in the different barrier-on-chip systems we develop. (uu.se)
  • Your main responsibilities will be to carry out and communicate top level scientific research in 1) on-chip cell cultures and 2) biological evaluation of barrier-on-chip microfluidic systems. (uu.se)
  • This research project demands a highly creative individual with strong analytical skills as well as practical skills regarding the handling of microfluidic systems and evaluation of cellular responses cultured in our various barrier-on-chip systems. (uu.se)
  • This month's issue of Cold Spring Harbor Protocols ( www.cshprotocols.org/TOCs/toc11_08.dtl ) features two articles with detailed instructions for setting up these experimental culture systems. (bio-medicine.org)
  • It was observed that overexpression of Id3 inhibited development of B cells in both culture systems. (bloodjournal.org)
  • The clinical course can be variable and involve almost all organ systems. (brainscape.com)
  • On the contrary, the cost associated with such technologies and systems are high and more preference is given to the 2D cell cultures. (medgadget.com)
  • In the attempt to avoid these disadvantages, transepithelial CXL (TE-CXL) technique was introduced, with a protocol based on a specially formulated riboflavin solution, Ricrolin TE (Sooft, Montegiorgio, FM, Italy), featuring two enhancers, trometamol and sodium EDTA, in order to improve stromal penetration. (hindawi.com)
  • The native stromal cells and extracellular matrix are typically removed, which enables isolated culture of the epithelial tissues. (nih.gov)
  • CD34 + CD38 − FL cells were transduced with Id3-IRES-GFP and cultured with the murine stromal cell line S17. (bloodjournal.org)
  • However, the number of vegetative propagation cycles for the shoot-tip technique used may not be sufficient to eliminate chimeras completely in the mutated populations. (fao.org)
  • Cronauer SS, Krikorian AD (1985b) Reinitiation of vegetative growth from aseptically cultured terminal floral apex of banana. (springer.com)
  • To address this challenge, we have developed an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a closed circulatory perfusion system, facilitating inter-organ responses. (nature.com)
  • Induced mutation (either by chemicals or irradiation), coupled with in vitro propagation technique such as shoot-tip culture, has been established as a tool to generate variation in a number of vegetatively propagated crops [2- (fao.org)
  • The project successfully adapted established protocols for mutation induction and subsequent propagation of in vitro cultured shoot tips in two Philippine banana cultivars. (fao.org)
  • Both genes are expressed in the epithelium of anterior digestive organs such as esophagus, proventriculus and gizzard. (nii.ac.jp)