The average molecular weights are often determined by intrinsic viscosity measurement or high-temperature gel permeation chromatography (HT-GPC), as it is state-of-the-art for semi-crystalline polyolefins. (fraunhofer.de)
To enable additive manufacturers to analyze and optimize molecular weight distribution and structure in photopolymer resins, Malvern Panalytical offers a Gel Permeation Chromatography (GPC) solution with advanced multi-detection through its Omnisec platform. (malvernpanalytical.com)
Liquid chromatography2
Over the past three decades, chromatography, specifically liquid chromatography, has become the technology of choice in the purification of biotherapeutics, due in large part to its resolving power. (bioradiations.com)
New purification schemes are constantly being introduced and optimized to try and find this ideal balance. (bioradiations.com)
Bio-Rad's NGC™ Chromatography Systems were designed with this is mind and provide a range of customizable chromatography systems that can increase purification capabilities and be adapted to meet growing throughput needs, obviating the need to buy a new instrument. (bioradiations.com)
And it is a fast and inexpensive technology compared with traditional chromatography, which is widely used in drug research, sample purification, and purification of natural products currently. (flashcolumns.com)
Though many methods are used in the process of obtaining pure proteins, chromatography is commonly used to separate complex biological mixtures and isolate proteins of interest prior to downstream characterization. (bioradiations.com)
Electrophoresis is often a companion technique to chromatography because it allows researchers an orthogonal method with which to visualize the protein species present in a sample. (bioradiations.com)
In the general column chromatography process, it is usually accompanied by lateral diffusion, which will greatly reduce the resolution, so when walking the column, you should try to shorten the column chromatography time, especially to avoid the column overnight. (flashcolumns.com)
In addition to the transformation of the synthetic route of the process, efficient column chromatography amplification technology is also a very critical step. (flashcolumns.com)
Mixed mode1
Various forms of chromatography, including affinity, ion exchange, and mixed mode, are now being utilized to ensure that the final product is as pure as can be without compromising yield. (bioradiations.com)
Method1
Flash column chromatography, also known as a flash column, is a fast separation method, but it does not sacrifice resolution as others have said. (flashcolumns.com)
Multiple1
The single peak in the chromatogram is resolved further into multiple bands on the SDS-PAGE gel. (bioradiations.com)
Application1
As a consequence, most methods of flash chromatographic application in the literature are developed using silica gel as the separation substrate. (flashcolumns.com)
Exchange chromatography1
The extracellular enzyme was purified to homogeneity from cell-free supernatant by ammonium sulphate precipitation followed by dialysis and ion exchange chromatography, recovery yield reduced from 100 to 22% and purification fold increased from 1 to 9.82. (walshmedicalmedia.com)
Specifically, this program is expected to advance the optimized measurements of proteins and peptides using MS and affinity capture proteomic analysis approaches and to establish a repository of well characterized and comprehensively annotated clinical reference materials along with respective proteomics data sets for these specimens. (nih.gov)
Yield2
The project introduced innovative concepts such as in-process monitoring and the automation of environmental parameter control to optimize product yield, quality and homogeneity. (europa.eu)
The project involved many innovative elements including online in-process monitoring and the automated control of environmental parameters to optimize product yield, quality and homogeneity. (europa.eu)
High2
Given the need for high-speed sample processing in clinical settings, applications centered on the 2-D gel proteomic platform (which has limited throughput in its current implementations) will not be considered responsive to this funding opportunity. (nih.gov)
The conditions for the fermentation of the PfCP-2.9-expressing P. pastoris (3N25) were optimized to achieve high levels of production. (others-signal.com)
Production2
Fermentations were performed in shake or bioreactor cultures and the production of flavor compounds was analyzed by GCMS, gas chromatography-olfactometry and Spectrum Sensory Analysis. (perfumerflavorist.com)
The CoMoFarm project was the first to look at plant-based production systems holistically, with a view to optimizing the entire production train from cell to pure protein, specifically focusing on pharmaceutical industry standards. (europa.eu)
Maximum1
Maximum protease concentration of 6723 U/mL under optimized fermentation conditions (molasses, corn steep liquor, pH 9.0, and 45°C). The results showed, there was no difference observed in bacterial growth and protease titer while replacing yeast extract with corn steep liquor so, in this way, about 90% cost of nitrogen source can be saved. (walshmedicalmedia.com)