Operon fusion. Medical search. Frequent questions

The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
Northern blot experiments performed together with reporter-gene fusion studies confirmed that both degS and degU genes are transcribed together as an operon. (uni-marburg.de)
In most gram-positive bacteria, this operon consists of five genes dltXABCD but the involvement of the first ORF ( dltX ) encoding a small protein of unknown function, has never been investigated. (frontiersin.org)
We, therefore constructed an in frame deletion mutant of dltX , without affecting the expression of the other genes of the operon. (frontiersin.org)
The genes encoding sakacin Q and its cognate immunity protein showed strong translational coupling, which was investigated in detail by analyzing the properties of a series of beta-glucuronidase fusions. (nofima.no)
Northern analysis of the cerRAI transcript provided evidence that the cer ORFs are co-transcribed, and that the cer operon contains two additional genes. (uth.edu)
These genes are located in a genetic context, tcpRXABCYD, which resembles a putative catabolic operon. (lookformedical.com)
Transcriptional fusion studies demonstrated that TcpR activates the expression of the tcp genes, responding specifically to 2,4,6-TCP. (lookformedical.com)
We examined the gene expression of the Escherichia coli cad operon, which consisted of the genes cadB and cadA (lysine decarboxylase), using cells possessing cadB-lacZ fusion gene. (researcher.life)
A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. (nyu.edu)
This operon consists of two genes: the first open reading frame (pre-etz) is of unknown function, whereas the second one (etz) encodes for isopentenyl transferase. (agri.gov.il)
The seven conserved enzymatic domains necessary for tryptophan (Trp) biosynthesis are encoded in seven genetic regions that are organized differently (whole-pathway operons, multiple partial-pathway operons, and dispersed genes) in prokaryotes. (healthweblognews.info)
Systematic sequencing of the Escherichia coli K-12 chromosome (GenBank entry U18997) has revealed the presence of an apparently complete operon of genes (the gspC-0 operon) similar to genes coding for components of the main terminal branch of the general secretory pathway (e.g., the Klebsiella oxytoca pulC-0 pullulanase secretion operon) and to related genes required for type IV pilus biogenesis. (pasteur.fr)
Thus, the gsp operon of E. coli K-12 includes at least two functional genes which, together with the rest of the operon, are probably not expressed under laboratory conditions. (pasteur.fr)
The purpose of the present study was to investigate the participation of pht genes in the regulation of the operons coded into the Pht cluster. (uanl.mx)
We conducted Northern blot, uidA fusions and reverse transcription-PCR assays of pht genes in several mutants unable to produce phaseolotoxin. (uanl.mx)
We obtained evidence that the phtABC genes also participate in the regulation of the phtD, phtM and phtL operons. (uanl.mx)

We demonstrate the power of the method by uncovering several novel features of an iconic gene regulatory program: the induction of Escherichia coli 's lac operon in response to a switch from glucose to lactose. (nature.com)
The glutamate synthase operon ( gltBDF ) contributes to one of the two main pathways of ammonia assimilation in Escherichia coli . (biomedcentral.com)
The gene encoding ArsR/methyltransferase fusion protein, arsR M , was amplified and expressed in Escherichia coli BL21 (DE3), and this strain showed resistance to arsenic in the present of 0.25-6 mM As(III), aresenate, or pentavalent roxarsone. (biomedcentral.com)
Regulation of the hydrogenase-4 operon of Escherichia coli by the sigma(54)-dependent transcriptional activators FhlA and HyfR. (ox.ac.uk)
The Escherichia coli ClpYQ (HslUV) complex is an ATP-dependent protease, and the clpQ + Y + ( hslV + U + ) operon encodes two heat shock proteins, ClpQ and ClpY, respectively. (openmicrobiologyjournal.com)
BirA, the biotin-protein ligase (BPL) of Escherichia coli, is also known as biotin operon repressor, biotin-[acetyl-CoA-carboxylase] ligase, and biotin-[acetyl-CoAcarboxylase] synthetase. (watson-int.cn)

In this work, it was demonstrated that, in a strain overexpressing σ 32 , the levels of groESL transcripts and the synthesis of GroEL are increased, confirming that this factor is responsible for the transcriptional activation of the σ 32 -like promoter of this operon, that also presents a inverted repeat called CIRCE in its regulatory region. (usp.br)
We show here that this promoter drives the expression of an operon consisting of a bacteriocin gene (sppQ), a cognate immunity gene (spiQ), another gene with an unknown function (orf4), and a pseudoimmunity gene containing a frameshift mutation (orf5). (nofima.no)
The transcriptional start of the tcp operon was mapped, and a putative sigma(70)-type promoter was identified. (lookformedical.com)
fusion gene was constructed, with the clpQ + Y + promoter fused to a lacZ reporter gene. (openmicrobiologyjournal.com)

Instead, this isolate has a horizontally acquired gene chimera that encodes a novel fusion protein with isopentyltransferase and phosphoribohydrolase domains, predicted to be capable of catalyzing and activating cytokinins, respectively. (usda.gov)
and encodes the full B. cepacia lipase operon for intracellular ex pression in E. coli. (faksignaling.com)
For example, the last gene in the gsp operon, gspO (formerly hopD), encodes a protein which is similar to several type IV prepilin peptidases. (pasteur.fr)
Another gene in the gsp operon, gspG (formerly hopG), encodes a protein similar to prePulG, a component of the pullulanase secretion pathway. (pasteur.fr)
H ducreyi contains a fimbrialike protein (Flp) operon that encodes proteins that contribute to adherence and pathogenesis. (medscape.com)

Plasmid-cured strains of E. amylovora exhibit reduced virulence, delayed symptom expression and altered exopolysaccharide (EPS) production on minimal medium with out thiamin (MM-T). EPS in E. amylovora is composed of two separate fractions, amylovoran (a pathogenicity factor) and levan (a virulence factor), and we have established a link between carriage of pEA29 and elevated expression of the amylovoran biosynthetic operon ( ams ) during immature pear fruit infection. (ishs.org)
The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with the fas operon central to virulence. (usda.gov)
The expression of the cad operon was reduced in cells containing the plasmid-encoding ornithine decarboxylase, which produced carbon dioxide, indicating that the gene expression of the cad operon was regulated by carbon dioxide (or its derivatives). (researcher.life)
Following insertion into plasmid pCD003 cleaved with XhoI and KpnI at the same time, plasmid pAT LipBc was obtained encoding a fusion protein comprising Inhibitors,Modulators,Libraries the signal peptide of CtxB at the N terminus followed from the lipase like a passenger, the linker region along with the B barrel in the AIDA I autotransporter necessary for outer membrane translocation and total surface accessi bility. (faksignaling.com)
The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)

A 3,011-bp arsenite resistance ars cluster arsHRNBC and a 5649-bp methionine biosynthesis met operon were found on the 3.315-Mb chromosome. (biomedcentral.com)
The operon for cytokinin biosynthesis of Erwinia herbicola pv. (agri.gov.il)
The operon for cytokinin biosynthesis in the gall-forming bacterium Erwinia herbicola pv. (agri.gov.il)
BirA functions both as a DNA-binding protein that represses the biotin biosynthesis operon as well as an enzyme that synthesizes its own corepressor, bio-5′-AMP, an intermediate in biotinylation reactions. (watson-int.cn)

In addition, the expression of these fusions are no longer cell-cycle regulated, as well as GroEL synthesis in a strain which does not have the HrcA protein, the putative repressor that binds CIRCE, indicating that the CIRCE-HrcA system are involved in cell cycle regulation of groESL in C. crescentus. (usp.br)
The objective of this study was to identify an environmental stimulus that influences the regulation of cerRAI and, to characterize transcription of the cer operon. (uth.edu)
According to our results, we propose that PhtABC and other Pht product activities could be involved in the synthesis of the sulfodiaminophosphinyl moiety of phaseolotoxin, which indirectly could be involved in the transcriptional regulation of the phtA operon. (uanl.mx)

The involvement of nearly half of E. coli 's most-global regulatory proteins in the control of gltBDF transcription is striking, but seems consistent with the central metabolic role of this operon. (biomedcentral.com)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

The expression of the groESL operon is induced by heat shock and is cell cycle controlled at normal temperatures, with maximal transcription in the predivisional cell and very low levels in the stalked cell. (usp.br)
The expression of a fos-lacZ fusion gene was studied in primary mouse epidermal keratinocytes obtained from transgenic mice. (elsevierpure.com)
This gene construct contains the entire upstream regulatory sequence of c-fos, and expression of the endogenous and fusion gene was shown by Northern analysis to correlate upon induction with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). (elsevierpure.com)
Using a chromogenic substrate of β-galactosidase, we also demonstrated that expression of the fusion gene product, like that of Fos, was localized to the cell nucleus. (elsevierpure.com)
In order to gain insight into the physiological role of the Hyf system, we investigated hyf expression by using a hyfA-lacZ transcriptional fusion. (ox.ac.uk)
Thus, hyf expression resembles that of the hyc operon. (ox.ac.uk)
The cad operon was expressed when O2 was limited, and the expression was optimal at pH 6.3. (researcher.life)
The expression vector was successful to over-express the 6xHis tagged-PilS fusion protein at a high level. (edu.hk)

We report here direct evidence that three of these seven proteins, plus a more specific regulator, cooperate to control an operon critical to nitrogen metabolism. (biomedcentral.com)
BirA biotinylates via the lysine side chain of biotin-accepting proteins/peptides, including natural substrate, carboxyl carrier protein (BCCP),and Avi Tag fusion proteins. (watson-int.cn)

Transcription analysis verified the key role of the catabolic operons located in the 92-kb scaffold, and flanked by transposases, on the transformation of OPP by S. haloaromaticamans . (nature.com)
groESL-lacZ transcription fusions with point mutations in CIRCE indicated a negative role of this cis-acting element only at normal growth temperatures, with a minor effect on heat shock induction. (usp.br)
lacZ transcriptional fusion in R. sphaeroides 2.4.1 wild type was tested under different environmental stimuli, such as various carbon sources, oxygen tensions, light intensities and culture media to determine if they influence transcription of the cer ORFs. (uth.edu)
Moreover, we have found that virF transcription is finely modulated by temperature and, with a call K-12 strains containing a virF-lacZ gene fusion, by H-NS. (uniroma3.it)
The chromosomal copies of gspG and gspO are apparently not expressed, probably because of very weak transcription from the upstream region, as measured by using a chromosomal gspC-lacZ operon fusion. (pasteur.fr)

lacZ translational fusion was created and was used to show that cerA can be translated. (uth.edu)
lacZ translational fusion autoinducer bioassay. (uth.edu)

2000). Membrane-fusion protein homologues in gram-positive bacteria. (tcdb.org)
The CAD gene appears to have evolved through a process of gene duplication and DNA rearrangement, leading to an in‐frame gene fusion encoding a chimeric protein. (uky.edu)
To allow protein purification, a gene fusion, which would encode the fusion protein with PilS attached to a His-tag, was prepared, and the tagged protein was purified by affinity methodology. (edu.hk)

The dlt operon of Gram-positive bacteria is required for the incorporation of D -alanine esters into cell wall-associated teichoic acids (TAs). (frontiersin.org)

lacZ transcriptional fusion was monitored in a subset of different global DNA binding transcriptional regulator mutant strains of R. sphaeroides 2.4.1. (uth.edu)
op), a transcriptional fusion gene, was used to form lysogens in the wild-type, rpoH or/and rpoS mutants. (openmicrobiologyjournal.com)

The arsHRNBC cluster and the metRFHH operon of M20 were identified by sequencing. (biomedcentral.com)
Future work should further explore how ArsR M regulates the met operon and the ars cluster. (biomedcentral.com)

The primers and probe were synthesized at Eurofins MWG Operon (Ebersberg, Germany). (cdc.gov)

Fusion of upstream DNA fragments of both pre-etz and etz to the ice nucleation reporter gene inaZ in pVSP61 showed high ice nucleation activity in both cultures, confirming the presence of two independent promoters. (agri.gov.il)

Identification of a pil operon in serovar Typhi suggested that this serovar strain might synthesize Type IV pili (Zhang et al. (edu.hk)
lacz fusion strain. (liverpool.ac.uk)

Genomic analysis identified two orthologous operons encoding the ortho -cleavage of benzoic acid (BA) ( ben/cat ). (nature.com)

Inactivation of the hyf operon had no discernible effect on growth under the range of conditions tested. (ox.ac.uk)

Studies of a pckA-lacZ gene fusion indicated that when cells were grown in minimal media with various carbon sources, such as succinate, malate, pyruvate, lactate, or ethanol, under both anaerobic light and aerobic dark conditions, the pckA gene was induced in log phase, irrespective of the carbon source. (asm.org)

Genes encoding HlyA, as well as other RTX toxins, are typically located within an operon with the arrangement hlyCABD . (medscape.com)
The hlyB and hlyD genes, along with the genetically unlinked tolC , encode an ABC transporter, membrane fusion/channel protein and outer membrane protein, respectively, which together form the type I secretion system through which HlyA is translocated. (medscape.com)
Among these, 11 single genes and 25 operons are likely to be under direct control of LutR. (microbiologyresearch.org)
We examined the gene expression of the Escherichia coli cad operon, which consisted of the genes cadB and cadA (lysine decarboxylase), using cells possessing cadB-lacZ fusion gene. (researcher.life)
The AGA58 chromosome composed of a single linear chromosome of 2,294,535 bp that is predicted to carry 2151 coding sequences, including 45 tRNA genes, 4 rRNA operons. (sciencegate.app)
In total, 12 transcriptional fusions with gfp were constructed corresponding to the nine checkpoint necessary genes of interest. (narturalproducts.com)
It was during her studies of the breakage-fusion-bridge cycle that she detected an unexpected burst of unstable genes in her stocks. (nih.gov)
The genes for the conversion of isophthalate and terephthalate to protocatechuate were cloned on a single fragment of genomic DNA from C. testosteroni YZW-D. The two operons were located by subcloning and mutant complementation experiments. (nih.gov)
Analysis of the nucleotide sequence for the isophthalate degradation operon located genes for a dioxygenase, a transport protein, a cis-dihydrodiol dehydrogenase, and a reductase. (nih.gov)
Analogous to the well-known lactose operon repressor (LacR), genes that encode for ROK repressors are frequently found on an operon alongside genes that function in the uptake and metabolic breakdown of a specific carbohydrate substrate. (nih.gov)

On the other hand, both transcriptional fusions that included an internal mdf 1 promoter revealed the same ubiquitous activities in early embryos. (narturalproducts.com)
228 - Activation of transcriptional fusions in Streptomyces lividans resulting from insertion of a 14-bp oligonucleotide - Nucleic Acids Res. (nih.gov)

To better understand the lack of osmoregulation of OmpC expression in S. typhi, we compared the expression of the ompC gene in S. typhi and E. coli, using ompC-lacZ fusions and outer membrane protein (OMP) electrophoretic profiles. (nih.gov)
Binding of integration host factor (IHF) to the Escherichia coli sodA gene and its role in the regulation of a sodA-lacZ fusion gene. (nih.gov)
We have also investigated the transcriptional regulation of sodA by monitoring the expression of a sodA-lacZ fusion gene in an IHF-negative E. coli strain under different growth conditions. (nih.gov)
This small gene overlaps with the beginning of the phoH2 gene in a manner similar to the PIN domain toxin-antitoxin operons. (nih.gov)
This allele is a protein fusion to the Venus YFP followed by the chloramphenicol resistance gene cat under its own promoter. (yale.edu)
The expression of the cad operon was reduced in cells containing the plasmid-encoding ornithine decarboxylase, which produced carbon dioxide, indicating that the gene expression of the cad operon was regulated by carbon dioxide (or its derivatives). (researcher.life)
Insertion on the foldase gene into pBL001 resulted in plasmid pAT FoldBc encod ing an in frame fusion of the autodisplay domains with fol dase as being a passenger. (ras-signal.com)
The only construct that did not drive ubiquitous GFP expression in early embryos is the putative promoter of mdf 1, which is in an operon, that extends upstream from the ATG initiator site in the first gene, his 35, of the operon to the adjacent upstream gene. (narturalproducts.com)
This concept was the precursor of the regulator-operon theory of gene regula- tion that won for its promulgators, Jacob and Monod, the Nobel Prize in 1965. (nih.gov)
The PvuII-HindIII fragment from the 5' end of the trp operon through nt 1999 of ECOTGP, which is in trpD CDS, was ligated to the HindIII-PvuII fragment of pBR322 containing the bla gene for Amp-resistance and origin of replication, but not the rop gene, which encodes a negative regulator of ColE1 replication. (addgene.org)
Yet in cells missing deletion bypasses the essentiality of exists within an operon with an upstream gene may encode a TA program. (researchassistantresume.com)
1998 We fused PD 151746 also to the T25 and T18 fragments respectively and co-expressed the gene fusions in appearance did PD 151746 not eliminate were identified just in the α-proteobacteria (Fig. S1C). (researchassistantresume.com)

MudI1734 fusion, but failed to stimulate transcription of the same fusion in a haploid genetic background. (nih.gov)
To understand the broader regulatory roles of LutR in B. subtilis , we studied the genome-wide effects of a lutR null mutation by combining transcriptional profiling studies using DNA microarrays, reverse transcription quantitative PCR, lacZ fusion analyses and gel mobility shift assays. (microbiologyresearch.org)
The S . Typhimurium σ 54 regulon included a high number of intragenic σ 54 binding sites/promoters, suggesting that σ 54 may have multiple regulatory roles beyond the initiation of transcription at the start of an operon. (biomedcentral.com)
gal-490 -- This mutation has an IS2 insertion element in the mRNA leader of the gal operon preventing transcription. (yale.edu)
Hereditary studies using a medication beliefs proB treA operon fusion news reporter stress revealed that proBA transcription is sensitively associated with proline supply and is also derepressed the moment cell phone misery pertaining to Hepatic inflammatory activity proline takes hold. (vegfr-signal.com)
Such repressors auto-regulate the operon they are contained on, activating transcription when the substrate for proteins encoded on the operon is present. (nih.gov)
Very often, the antitoxin binds to a palindromic stretch within the promoter region and represses the transcription of the operon. (centralparkcarriagesofficial.org)

Transcriptional regulation of prp was studied using MudI1734(lacZ+) operon fusions. (nih.gov)
Cell aggregation is dependent on the EPS poly- N -acetylglucosamine (PNAG), thus suggesting negative regulation of the PNAG biosynthetic operon pgaABCD by PNPase. (biomedcentral.com)

In this case, the sec- ond hybrid is a fusion between the GAL4 AD and proteins encoded by genomic (q.v.) or cDNA library (q.v.) sequences. (damasgate.com)

Complementation experiments with either the S. typhi or E. coli ompB operon in an S. typhi DeltaompB strain carrying the ompC-lacZ fusions showed that both S. typhi and E. coli ompC were not regulated by osmolarity when they were under the control of S. typhi ompB. (nih.gov)

Yakimov MM, Kroger A, Slepak TN, Giuliano L, Timmis KN, Golyshin PN: A putative lichenysin A synthetase operon in Bacillus licheniformis: initial characterization. (pparpathway.com)

In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

Genetic fusion of the major birch pollen allergen (Bet v1) to bacterial surface-(S)-layer proteins resulted in recombinant proteins exhibiting reduced allergenicity as well as immunomodulatory capacity. (biomedcentral.com)

Thus, our present results suggested that the physiological role of the cad operon is to supply carbon dioxide when its internal level is lowered under O2-limiting conditions at a low pH. (researcher.life)

This fusion of a PIN domain with an ATPase reflects a much wider association between PIN domains and PhoH domains across many prokaryote genomes. (nih.gov)

Buy Ditropan 5Mg Online Canada Wu ditropan 2,5mg online B, Song J, Beitz E (2010) Novel channel enzyme fusion proteins confer arsenate resistance. (centralparkcarriagesofficial.org)

Interestingly, this structure and conformational changes are conserved in ROK kinases, which implies that the evolution of ROK repression elegantly arose by the simple fusion of DNA binding domains and the coopting of pre-existing induced fit conformational changes. (nih.gov)

Anatomy3

Organisms5

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment11

Phenomena and Processes46

Information Science3

Genes17

Escherichia6

Promoter4

Encodes5

Plasmid5

Biosynthesis4

Regulation3

Coli2

Expression8

Proteins2

Transcription5

Translational2

Protein3

Gram-positive1

Transcriptional fusion2

Cluster2

Primers1

Upstream1

Strain2

Analysis1

Growth1

Conditions1

Genes10

Transcriptional fusions2

Gene12

Transcription7

Regulation2

Proteins encoded1

LacZ1

Bacillus1

Subtilis1

Genetic1

Role1

Domain1

Resistance1

Domains1