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OocytesOogenesisOocyte DonationOocyte RetrievalXenopus laevisMeiosisIn Vitro Oocyte Maturation TechniquesXenopusMicroinjectionsFertilizationMetaphaseCumulus CellsParthenogenesisBlastocystOvumSperm-Ovum InteractionsCryopreservationOvarySperm Injections, IntracytoplasmicEmbryonic DevelopmentEmbryo TransferZona PellucidaMaturation-Promoting FactorSpermatozoaZygoteCleavage Stage, OvumEmbryo Culture TechniquesRNA, ComplementaryPregnancyEgg ProteinsNuclear Transfer TechniquesFollicular FluidOvulation InductionCytoplasmProto-Oncogene Proteins c-mosCryoprotective AgentsVitrificationCell NucleusPolar BodiesEmbryo, MammalianRNA, MessengerEmbryonic and Fetal DevelopmentPregnancy RateInfertility, FemaleChorionic GonadotropinMolecular Sequence DataOvulationGranulosa CellsCells, CulturedXenopus ProteinsCattleMorulaProgesteroneMeiotic Prophase IReproductive Techniques, AssistedSpindle ApparatusChromosomesEthylene GlycolAmino Acid SequenceVitellogenesisSwineGenes, mosProphaseBase SequenceGonadotropinsSuperovulationGrowth Differentiation Factor 9BlastomeresBone Morphogenetic Protein 15Gene Expression Regulation, DevelopmentalCDC2 Protein KinaseTime FactorsSperm HeadAneuploidyCloning, MolecularMicrotubulesCulture MediaReproductive TechniquesEmbryo ImplantationMembrane PotentialsMice, Inbred ICRInfertilityEctogenesisMicroscopy, ConfocalGonadotropins, EquinePatch-Clamp TechniquesCyclin BFertilityCell AgingProtein BiosynthesisCholestenesMice, Inbred StrainsPropylene GlycolKineticsRecombinant ProteinsPregnancy OutcomeTissue and Organ HarvestingCalciumCell MembraneEstradiol
EmbryosCryopreservationMammalianOvariesRetrievalImmatureIntracytoplasmic sperm injComplexesPorcine in vitro matuOvumCompetenceICSIGranulosa cellsVitrificationFolliclesEmbryonicMature oocytesFollicularIncreased oocyte maturationVivoGerm cellsHuman oocytesCattle oocyteEffects on oocyteUnderwentSpermDonorSimilar in vitroPrimary oocytesPregnancyDonationAbstractMelatoninMaturation viaMeioticTransvaginalMeiosisEquineApoptosisEmbryo TransferOutcomesZonaEarly embryoCyclesMembraneXenopusConclusionOvarySupplementationNuclear
Embryos19
  • The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. (springer.com)
  • We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. (scite.ai)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • Utrecht can do ovum pick-up from mares and collaborate with Avantea, Italy for in vitro production and cryopreservation of embryos. (wur.nl)
  • Higher percentage of MII stage oocytes in experiment 1(65.2±2.0), embryos at morula stage in experiment 2 (30.4±1.5) and experiment 3 (22.2±2.0) were obtained. (bvsalud.org)
  • The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
  • Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
  • Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
  • To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
  • Experimental spindle transfer in metaphase II oocytes, also called mitochondrial replacement therapy, is a novel technology for preventing mtDNA transmission from oocytes to pre-implantation embryos. (infertile.com)
  • Reprogramming was demonstrated by the capacity of the reconstructed embryos to develop to the blastocyst stage in vitro and into fetuses and viable offspring in suitable foster mothers. (cnrs.fr)
  • However, other procedures based on the in vivo and in vitro production of equine embryos have emerged in recent years. (ivis.org)
  • Reasons for the delayed development of in vivo and in vitro production of equine embryos include the scarce availability of abattoir ovaries and the lack of interest from horse breeders and registries. (ivis.org)
  • Assisted reproductive techniques (ARTs) involve manipulation of sperm and ova or embryos in vitro with the goal of producing a pregnancy. (msdmanuals.com)
  • [ 3-6 ] It is widely assumed that this improvement is related to the beneficial effects of GH on oocyte quality, as suggested by the observations of a higher number of oocytes collected, higher fertilization rate, and a higher number of embryos reaching the transfer stage in GH-treated patients. (medscape.com)
  • however, the 3 groups were similar with respect to the cause of infertility, total dose of follicular stimulating hormone (FSH), number of oocytes and embryos obtained, and number and quality of embryos transferred. (who.int)
Cryopreservation8
Mammalian5
  • Sperm/oocyte membrane interaction in mammalian fertilization remains poorly understood. (escholarship.org)
  • Mammalian oocytes represent impaired quality after undergoing a process of postovulatory aging, which can be alleviated through various effective ways such as reagent treatment. (scite.ai)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • After successfully overcoming the problem of making mammalian oocytes mature in vitro in 1965, Edwards began to experiment with fertilizing matured eggs in vitro. (asu.edu)
  • Mammalian oocytes are arrested in the dictyate stage of meiotic prophase I for long periods of time, during which the high concentration of the p53 family member TAp63α sensitizes them to DNA damage-induced apoptosis. (elifesciences.org)
Ovaries7
  • Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. (tubitak.gov.tr)
  • A total of 881 cumulus-oocyte complexes (COC's) were collected from 243 ovaries. (ac.ir)
  • Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory . (bvsalud.org)
  • Detection of DNA damage in oocytes of small ovarian follicles following phosphoramide mustard exposures of cultured rodent ovaries in vitro. (hal.science)
  • Using a model ovotoxic alkylating drug, cyclophosphamide (CPA), and its active metabolite, phosphoramide mustard (PM), we previously showed that PM (≥3μM) caused significant follicle loss in postnatal day 4 (PND4) mouse ovaries in vitro. (hal.science)
  • Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. (infertilityschool.ru)
  • Histology evaluation: Tiny ovaries with no growing oocytes or follicles (see 3). (jax.org)
Retrieval12
  • Without gonadotropin priming and HCG trigger, ultrasound-guided transvaginal oocyte retrieval was performed. (springer.com)
  • Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
  • Transvaginal oocyte retrieval (TVOR), also referred to as oocyte retrieval (OCR), is a technique used in in vitro fertilization (IVF) in order to remove oocytes from the ovary of a woman, enabling fertilization outside the body. (wikipedia.org)
  • Transvaginal oocyte retrieval is more properly referred to as transvaginal ovum retrieval when the oocytes have matured into ova, as is normally the case in IVF. (wikipedia.org)
  • There are significantly improved outcomes when women are exposed to seminal plasma around the time of oocyte retrieval, with statistical significance for clinical pregnancy, but not for ongoing pregnancy or live birth rates with the limited data available. (wikipedia.org)
  • pregnancy rates with this technique are lower than those with retrieval of multiple oocytes, but costs are lower and success rates are increasing. (msdmanuals.com)
  • Participants provided urine samples during ovarian stimulation and on the day of oocyte retrieval. (cdc.gov)
  • Egg retrieval (also called oocyte retrieval). (cdc.gov)
  • Based on our models, a new patient who is 35 years, with a BMI of 25 kg/m(2), no previous pregnancies, and unexplained infertility diagnoses has a 48%, 69%, and 80% CLBR following first, second, and third oocyte retrieval. (cdc.gov)
  • Conscious sedation and analgesia are one of several methods used to relieve pain during oocyte retrieval in IVF procedures. (bvsalud.org)
  • The information in this study is of value when considering the management of pain in patients undergoing oocyte retrieval procedures. (bvsalud.org)
  • These data suggest that administration of silymarin in IVF patients concomitantly with gonadotropin results in reduction of granolusa cell apoptosis but does not have any effect in promotion of follicular development, oocyte retrieval or endometrial thickness. (who.int)
Immature5
  • After oocyte collection, immature oocytes were cultured in the IVM medium. (springer.com)
  • Illustrative example of progress made is the world-first foal born in 2017 after development of an improved vitrification protocol for equine immature oocytes. (wur.nl)
  • Therefore, a small biopsy of ovarian cortex tissue was performed using laparoscopy to retrieve immature oocytes. (infertilityschool.ru)
  • Four immature, germinal vesicle (GV) oocytes were found and removed from tissue, denuded mechanically by a pipette, and matured in vitro in a maturation medium with added FSH and hCG as well as in co-culture with cumulus cells, which were retrieved by their denudation. (infertilityschool.ru)
  • In immature egg cells (also known as oocytes), p63 mostly exists in an inactive form. (elifesciences.org)
Intracytoplasmic sperm inj3
  • Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). (springer.com)
  • We investigated whether the insemination method ( in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. (ecerm.org)
  • At this point, intracytoplasmic sperm injection-injection of a single sperm into each oocyte-may be done, particularly if spermatogenesis is abnormal in the male partner. (msdmanuals.com)
Complexes6
  • Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • In order to compare the degree of meiotic competence, MII oocytes obtained adopting an advanced IVM, consisting of cumulus oocytes complexes co-cultured with walls antral follicle-derived somatic cells, were analyzed. (unipg.it)
  • 2010. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. (ipb.ac.id)
  • ScholarWorks@Gyeongsang National University: Coculturing cumulus oocyte complexes with denuded oocytes during in vitro maturation affects oocyte maturation, zona hardening, polyspermy and oocyte GPX 1 gene expression. (gnu.ac.kr)
Porcine in vitro matu2
  • The thesis investigated the various factors influencing porcine in vitro maturation of an oocyte (IVM), fertilization (IVF), and early embryo culture (IVC). (uconn.edu)
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
Ovum3
  • Because of the large numbers, this work provides insights into factors affecting success rates of ovum pick-up and in vitro embryo production. (wur.nl)
  • Oocyte maturation and ovum quality in pigs. (ipb.ac.id)
  • A female reproductive cell, also called an oocyte or ovum. (cdc.gov)
Competence4
  • Moreover, PMSG promoted a complete meiotic competence in the majority of collected oocytes (58.3% MII, 2.8% GVBD, 38.8% GV). (unipg.it)
  • 7) the developmental competence of porcine oocytes was significantly affected by an interaction among the age of the donor animal, ovarian follicle size, and exogenous gonadotropic stimulation. (uconn.edu)
  • 2011. Effect of leptin during in vitro maturation of prepubetal calf oocytes: embryonic development and relative mRNA abudances of genes involved in apoptosis and oocyte competence. (ipb.ac.id)
  • Co-culture with follicular shell pieces(FSP)increases the development al competence of pig oocytes matured in vitro. (elsevierpure.com)
ICSI4
  • 2009. Effect of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. (ipb.ac.id)
  • The morphokinetics of embryo development was found to vary between IVF- and ICSI-fertilized oocytes, at least until the 6-cell stage. (ecerm.org)
  • ICSI produced the first pregnancy derived from an in vitro matured oocyte [ 5 ], and it was successfully carried to term. (ivis.org)
  • This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
Granulosa cells5
Vitrification9
  • The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. (jove.com)
  • Juvenile oocyte vitrification was a lot shorter in the generation interval in breeding programs. (jove.com)
  • The protocol include all the procedures from in vitro maturation, vitrification, warming, and post-warming culture. (jove.com)
  • The selection of the oocytes to submit at the vitrification procedures represent the first important step to warranty the successful application of this procedure. (jove.com)
  • The loading of the oocyte in the vitrification device used is an important and critical step. (jove.com)
  • In this method, the oocytes in the vitrification solution, less than 0 point milliliter are rapidly loaded with a glass capillary on top of the film strip. (jove.com)
  • Prof. Ann van Soom of the Faculty of Veterinary Medicine, Ghent University, presented an overview of the major progress made in the past years in vitrification, oocyte and embryo culture in horses, cats and cattle. (wur.nl)
  • Human oocyte vitrification works very well, further work on oocyte vitrification in cattle, horse, cat can learn from human. (wur.nl)
  • Various factors important for successful vitrification of bovine and equine oocytes were addressed. (wur.nl)
Follicles6
  • Because of a concern exists on the possible re-introduction of malignant cells following ovarian transplantation, in vitro (iv) culture of early stage follicles still remains a potential alternative to obtain fertilizable oocytes that contribute to biological offspring. (unipg.it)
  • Since technologies advances are required to transfer iv Follicologenesis (ivF) from bench to bedside, monovulatory large mammal becomes ideal translational models to validate cultural conditions enhabling synergic follicles-oocyte iv development. (unipg.it)
  • Of note, the MII oocytes were all isolated by early antral follicles with Δ%>40 (p<0.01). (unipg.it)
  • When the ovarian follicles have reached a certain degree of development, induction of final oocyte maturation is performed, generally by an intramuscular or subcutaneous injection of human chorionic gonadotropin (hCG). (wikipedia.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • Lauritzen, J. G. Ultrasonically guided percutaneous aspiration of human follicles under local anesthesia: a new method of collecting oocytes for in vitro fertilization . (bvsalud.org)
Embryonic3
Mature oocytes1
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
Follicular6
  • Once the follicle is entered, suction is carefully applied to aspirate follicular fluid containing cellular material, including the oocyte. (wikipedia.org)
  • Follicular flushing has not been found to increase pregnancy rates, nor result in an increase in oocyte yield. (wikipedia.org)
  • 2017. Insuline-Like groeth factor-I concentration in the follicular fluid of bali cattle and its role in the oocyte nuclear maturation and fertilization rate. (ipb.ac.id)
  • observed that heat stress during the follicular recruit- the regulating mechanism of oocyte maturation. (sagepub.com)
  • EVs in the follicular fluid, which fills the ovarian follicle and influences oocyte developmental competency, carry microRNAs (EV-miRNAs) that have been associated with In Vitro Fertilization (IVF) outcomes. (cdc.gov)
  • ABSTRACT To investigate the effects of silymarin on follicular development, we enrolled 40 healthy women undergoing in vitro fertilization (IVF) due to male factor infertility in this trial. (who.int)
Increased oocyte maturation1
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
Vivo6
  • The purpose of this study was therefore to compare treatment of P with DHP on batch level, in vitro, to determine dose-response effects, and in vivo, at a single dose. (wur.nl)
  • Our results support the conclusion that DHP and P work equally well in vitro and in vivo. (wur.nl)
  • Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
  • Today, the most practical use of OPU or transabdominal follicle aspiration is to recover in vivo matured oocytes [ 9 ] for oocyte transfer. (ivis.org)
  • Mit Hilfe von Generationen-übergreifenden Mausmodellen, möglichst in einem translationalen Ansatz durch Kombination mit epidemiologischen Daten ( Plattform Kohorten ) und in-vitro oder ex-vivo Zellkulturmodellen ( Plattform Zellkultur-Modelle , Ernährungsphysiologische Immunologie ), wollen wir die Auswirkungen früher chemischer Expositionen auf die Krankheitsentwicklung im späteren Leben untersuchen und die zugrunde liegenden Mechanismen charakterisieren. (ufz.de)
  • We found that the beta1 integrin null eggs were fully functional in fertilization both in vivo and in vitro. (lu.se)
Germ cells1
Human oocytes1
Cattle oocyte1
  • Vitrifaction by ultrarapid cooling and warming is considered a standard approach in cattle oocyte preservation. (jove.com)
Effects on oocyte2
  • As propofol has been shown to have deleterious effects on oocyte fertilization (in a mouse model), some authors have suggested that the dose of propofol administered during anesthesia should be limited, and also that the retrieved oocytes should be washed free of propofol. (wikipedia.org)
  • HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
Underwent3
  • G), with a long history of multiple undiagnosed pregnancy losses and deaths of offspring as a result of this disease, who underwent IVF after reconstitution of her oocytes by spindle transfer into the cytoplasm of enucleated donor oocytes. (infertile.com)
  • STUDY DESIGN: Our population-based cohort study utilized data from the National Assisted Reproductive Technology Surveillance System 2016-2018, including 196,916 women who underwent 207,766 autologous embryo transfer cycles and 25,831 women who underwent 36,909 donor oocyte transfer cycles. (cdc.gov)
  • Design and Study Population A total of 105 infertile women were enrolled in the randomized controlled trial: 70 women were with a history of RIF with donated oocytes, and 35 infertile women underwent the first oocyte donation attempt. (medscape.com)
Sperm12
  • The IZUMO1 protein and its receptor is the only sperm/oocyte interaction pair known to date that is necessary for gamete fusion in mice. (escholarship.org)
  • The current study aims to find potential candidate proteins for sperm/oocyte plasma membrane interaction in a porcine model. (escholarship.org)
  • First, sperm and oocyte plasma membrane proteins with affinity for proteins of the plasma membrane of their partner gamete were separated by affinity chromatography. (escholarship.org)
  • Approximately 100 oocyte plasma membrane proteins with affinity for the sperm, and 100 sperm plasma membrane proteins with affinity for the oocyte were identified with the two MS approaches. (escholarship.org)
  • In the IVF experiments, antibodies targeting oocyte proteins lipoprotein E (ApoE) and complement protein 6 (C6) significantly decreased the fertilization rate, suggesting that ApoE and C6 have a relevant role in the sperm/oocyte plasma membrane interaction. (escholarship.org)
  • This study complements the knowledge from previous analyses on gamete interactions by using a molecular-affinity-driven process to identify sperm/oocyte plasma membrane interacting components. (escholarship.org)
  • Dr. Bernard Roelen of the Faculty of Veterinary Medicine, Utrecht University, explained what can be expected from production of sperm and oocytes in vitro, on the basis of the latest international scientific progress in mice. (wur.nl)
  • In vitro clinic: Very low testis weight, no epididymal sperm recovered. (jax.org)
  • In vitro clinic: Low epididymal sperm concentration, many sperm have abnormally shaped heads, sperm tails are short, no sperm motility, no IVF success. (jax.org)
  • For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)
  • The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)
  • After sperm are added, the oocytes are cultured for about 2 to 5 days. (msdmanuals.com)
Donor9
  • It is recommended to tell recipients that previously cryopreserved donor oocytes are a reasonable option compared with fresh donor oocytes, given that there is good evidence that there are no significant differences in per transfer pregnancy rates compared with those with fresh donor oocytes. (guidelinecentral.com)
  • Recipients can be counseled that as the number of donor oocytes warmed increases, there is an associated increase in cumulative live birth rate. (guidelinecentral.com)
  • There is insufficient evidence to recommend a particular stimulation protocol for oocyte donors or certain donor characteristics in terms of embryo quality or success rates. (guidelinecentral.com)
  • Recipients can be counseled that, based on limited evidence, neonatal outcomes appear similar between vitrified and fresh donor oocytes. (guidelinecentral.com)
  • There are no significant differences in per transfer pregnancy rates with cryopreserved vs. fresh donor oocytes. (guidelinecentral.com)
  • Spindle transfer from the patient's metaphase II oocyte into the cytoplasm of a donor oocyte. (infertile.com)
  • D) transfer of the removed spindle into the perivitelline space of the enucleated donor oocyte. (infertile.com)
  • Title : Trends and Outcomes for Donor Oocyte Cycles in the United States, 2000-2010 Personal Author(s) : Kawwass, Jennifer F.;Monsour, Michael;Crawford, Sara;Kissin, Dmitry M.;Session, Donna R.;Kulkarni, Aniket D.;Jamieson, Denise J. (cdc.gov)
  • Among patients using donor oocytes, we estimated CLBR after their first, second, and third embryo transfers. (cdc.gov)
Similar in vitro2
  • Yamanaka's Nobel Prize-winning research paved the way for similar in vitro development from induced pluripotent stem cells (iPSCs). (wur.nl)
  • Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. (wur.nl)
Primary oocytes2
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • In mammals, up to 10 diverse p63 isoforms exist with the longest one, TAp63α, being highly expressed in primary oocytes that are arrested in prophase of meiosis I. After homologous recombination, oocytes are kept in this dictyate arrest phase until they are recruited for ovulation, a period that can take decades in humans. (elifesciences.org)
Pregnancy4
  • We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. (springer.com)
  • Recipients can be counseled that the length of time that oocytes have been stored is not associated with differences in oocyte survival or pregnancy rates. (guidelinecentral.com)
  • To assess it, we studied whether GH administration can improve the chance of pregnancy and birth in women who experienced repeated implantation failure (RIF) using donated oocyte programs. (medscape.com)
  • However, no correlation were categorised into 2 groups: 73 infertile has been found between these antibodies women presenting for an infertility evalu- and the number of oocytes and pregnancy ation, with a mean duration of infertility rate [ 6 ]. (who.int)
Donation1
Abstract1
  • Abstract The objective of the current study was to investigate the synergistic impact of - Tocopherol and - Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes . (bvsalud.org)
Melatonin4
  • It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
  • Conclusions: This study compared the efficiencies of resveratrol, melatonin and their combination for protecting porcine oocytes from heat stress. (sagepub.com)
  • Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)
  • In addition, its efficiency against oocyte had an adverse effect on their developmental com- HS will be compared with melatonin, another potent anti- petence by increasing GSH content and inhibiting the pro- oxidant, and the combination of melatonin with resveratrol. (sagepub.com)
Maturation via1
  • 2004. Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. (ipb.ac.id)
Meiotic4
  • However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP . (wikipedia.org)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)
  • These mutations cause a range of folding defects in vitro, different degrees of microtubule disruption upon expression in cultured cells and interfere to varying extents in the proper assembly of the meiotic spindle in mouse oocytes. (bmj.com)
Transvaginal1
  • Conscious sedation is the most common method of pain relief used during ultrasound-directed transvaginal follicle aspiration (UDFA) for in-vitro fertilisation/embryo transfer (IVF /ET). (bvsalud.org)
Meiosis4
  • Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)
  • Removal of the oocyte from the follicle causes meiosis to progress in the oocyte. (wikipedia.org)
  • The midcycle LH surge initiates several physiologic actions including: ovulation, resumption of meiosis in the oocyte, and luteinization. (nih.gov)
  • Conclusions Our data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8 , underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF). (bmj.com)
Equine1
  • Moreover, in spite of the early success of equine oocyte in vitro maturation [ 2 ], no relevant research on the issue has been reported in the horse for some time. (ivis.org)
Apoptosis3
  • Moreover, inhibition in apoptosis and autophagy of aged oocyte by Ax was confirmed through decreased caspase-3, cathepsin B and autophagic activities. (scite.ai)
  • The number and quality of oocytes retrieved were evaluated and apoptosis of granolusa cells was studied. (who.int)
  • Therefore, apoptosis of (ER)-mediatedactivity.Silybin-Aand day)andtheothergroupgonadotropin granolusacellsseemstohaveanegative otherflavonolignansareinactive,and plusplacebo.Transvaginalultrasound effectonfolliculardevelopment,oocyte taxifolin,whichisaminorconstituent examinationwasperformedtoevaluate qualityandpregnancyrates[ 2 ]. (who.int)
Embryo Transfer1
  • Since the first in vitro fertilization-embryo transfer (IVF-ET) baby was born in 1978, IVF-ET procedures have made remarkable progress, and various assisted reproductive technologies have been implemented. (ecerm.org)
Outcomes3
  • Infertile women should be counseled that, based on a small number of births, neonatal outcomes appear similar after using their own previously cryopreserved oocytes compared with outcomes after the use of fresh oocytes. (guidelinecentral.com)
  • Neonatal outcomes appear similar with cryopreserved oocytes. (guidelinecentral.com)
  • The ivF outcomes obtained by using the specie-specific (oFSH) or the trans-species chorionic gonadotropin (PMSG) were analyzed after 14 days of culture by comparing follicle/oocyte growth, timing and percentage (%) of antrum differentiation as well as % of Methaphase II (MII) oocytes after iv Maturation (IVM). (unipg.it)
Zona1
  • Since it is formed at an early stage in women harbouring ASA and AZP-Ab in of oocyte growth, zona pellucida-specific their cervical secretions. (who.int)
Early embryo1
  • Background TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. (bmj.com)
Cycles1
  • We used data on autologous IVF cycles to develop models that estimate a patient's cumulative live birth rate (CLBR) following all embryo transfers (fresh and frozen) within 12 months after one, two, and three oocyte retrievals in new and returning patients. (cdc.gov)
Membrane4
  • In addition, a library of porcine oocyte membrane proteins was generated containing more than 200 proteins. (escholarship.org)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • [5] The cyclic AMP is generated by the oocyte by adenylyl cyclase in the oocyte membrane. (wikipedia.org)
  • The adenylyl cyclase is kept active by a constitutively active G-protein-coupled receptor known as GPR3 and a G-protein, Gs, also present in the oocyte membrane. (wikipedia.org)
Xenopus3
  • In this paper we define the mechanism of inactivation for sqKv1A channels in Xenopus oocytes to investigate these and other discrepancies. (nih.gov)
  • Inactivation of sqKv1A in Xenopus oocytes was found to be unaffected by genetic truncation of the N-terminus, but highly sensitive to certain amino acid substitutions around the external mouth of the pore. (nih.gov)
  • To investigate the mechanism of the egl-2(gf) phenotype and rescue by imipramine, we analyzed EGL-2 currents in Xenopus oocytes. (jneurosci.org)
Conclusion4
  • In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
  • In conclusion, Ax might provide an alternative for ameliorating the oocyte quality following aging in vitro, through the mechanisms mediated by its antioxidant properties. (scite.ai)
  • In conclusion, this study provided a valuable assessment of the factors involved in porcine in vitro oocyte and embryo culture system. (uconn.edu)
  • In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate. (ipb.ac.id)
Ovary2
  • Galvão A, Segers I, Smitz J, Tournaye H, De Vos M. In vitro maturation (IVM) of oocytes in patients with resistant ovary syndrome and in patients with repeated deficient oocyte maturation. (springer.com)
  • Using a combination of biophysical methods as well as cell and ovary culture experiments we explain how TAp63α is kept inactive in the absence of DNA damage but causes rapid oocyte elimination in response to a few DNA double strand breaks thereby acting as the key quality control factor in maternal reproduction. (elifesciences.org)
Supplementation4
  • After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
  • The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. (ipb.ac.id)
  • However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. (ipb.ac.id)
  • Supplementation of - tocopherol in maturation media having - Linolenic acid and/or in embryo culture media did not further enhance in vitro maturation of oocyte or embryo production . (bvsalud.org)
Nuclear2
  • Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
  • At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. (ipb.ac.id)