Oocytes rna. Medical search. Frequent questions

Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and retina. (aspetjournals.org)
Electrical recordings were made in Xenopus oocytes to study the modulatory effects of steroids on gamma-aminobutyric acid (GABA) receptors expressed by RNA from mammalian brain and retina. (aspetjournals.org)
Transport of 16 substrates was measured for each individual human OATP in complementary RNA-injected Xenopus laevis oocytes. (nih.gov)
Xenopus oocyte expression system was used for functional analysis. (nih.gov)
To investigate the mechanism of the egl-2(gf) phenotype and rescue by imipramine, we analyzed EGL-2 currents in Xenopus oocytes. (jneurosci.org)
Studies using Xenopus cell-free extracts, oocytes, eggs, embryos, larval stages, and adult frogs have yielded important insights into a multitude of key biological processes-from mechanisms underlying the cell cycle to embryonic development to human disease. (cshlpress.com)
Avermectin-sensitive chloride currents induced by Caenorhabditis elegans RNA in Xenopus oocytes. (aspetjournals.org)
These snoRNAs were used in cross-linking studies to identify which proteins assembled on them in vivo in Xenopus oocytes. (fishersci.com)
The Na+/glucose cotransporter from rabbit intestinal brush border membranes has been cloned, sequenced, and expressed in Xenopus oocytes. (xenbase.org)
In this study, we have compared and contrasted the transport properties of this cloned protein expressed in Xenopus oocytes with the native transporter present in rabbit intestinal brush borders. (xenbase.org)
Initial rates of 14C-alpha-methyl-D-glucopyranoside uptake into brush border membrane vesicles and Xenopus oocytes were measured as a function of the external sodium, sugar, and phlorizin concentrations. (xenbase.org)
Expression of size-selected mRNA encoding the intestinal Na/glucose cotransporter in Xenopus laevis oocytes. (xenbase.org)
We have analyzed the expression and intracellular distribution, during oogenesis and embryogenesis, of Vg1 RBP, a protein implicated in the intracellular localization of Vg1 mRNA to the vegetal cortex of Xenopus oocytes. (zfin.org)
Although Vg1 RBP RNA is detected in all blastomeres of the early embryo, the expression pattern in the one day old zebrafish embryo is almost identical to that of the equivalent stage Xenopus embryo. (zfin.org)
Influenza D virus M2 protein exhibits ion channel activity in Xenopus laevis oocytes. (cdc.gov)
The mRNA of the DM2-cMyc gene was synthesized and injected into Xenopus oocytes. (cdc.gov)
Assuming the same expression level in the Xenopus oocytes, DM2 without tag and influenza C virus M2 protein (CM2) were subjected to the TEVC method. (cdc.gov)
Here we used two-electrode voltage clamp electrophysiology to compare the actions of CBD with those of the major central endocannabinoid, 2-arachidonoyl glycerol (2-AG) on human recombinant GABAA receptors (synaptic α1-6βg2 and extrasynaptic α4β2δ) expressed on Xenopus oocytes. (researchgate.net)
Fully-automated Roboinject system for automated injection of RNA, DNA or other samples into Xenopus oocytes or cells of similar size. (multichannelsystems.com)
Fully-automated Roboocyte2 system for screening of ligand-gated and voltage-gated ion channels, as well as electrogenic transporters based on the standard Xenopus oocyte expression system. (multichannelsystems.com)
Automated Voltage-Clamp Screening System for Xenopus laevis oocytes. (multichannelsystems.com)

The aim of this study was to isolate a rat counterpart of human LST-1 and examine the expression regulation of its messenger RNA (mRNA) to clarify the molecular basis of cholestasis. (nih.gov)
To determine the effect of TGF-beta1 on mRNA levels of several key effectors of oocyte maturation, three sets of experiments were performed. (biomedcentral.com)
However, little is known about the roles of PABPs in oocytes because of the unique mechanisms of mRNA metabolism in oocytes. (go.jp)
RNA-seq analysis revealed aberrant mRNA persistence in Pabpn1l KO-MII oocytes, which indicates impaired mRNA degradation during the germinal vesicle (GV) to MII transition. (go.jp)
We also revealed that the exogenous expression of Pabpn1l mRNA in KO-GV oocytes recovered defects of embryonic development. (go.jp)
The DM2-cMyc mRNA-injected oocytes were subjected to the two-electrode voltage-clamp (TEVC) method, and the induced inward current was observed. (cdc.gov)
Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
Different classes of RNA granules share some protein components and may use similar mechanisms to regulate mRNA translation/decay. (rupress.org)
We amplified the total mRNA and observed DNA fragments longer than 5000 bp in length, suitable for single-cell RNA sequencing. (auburn.edu)

A research group focused on embryos has begun its work in Finland, comprehensively surveying for the first time the short RNA molecules that regulate genome function during embryonic development. (helsinki.fi)
Protein-coding RNA molecules in human embryos have been studied earlier , but research on non-coding RNA molecules is extremely limited. (helsinki.fi)
The researchers investigated what kind of RNA molecules of 18 to 30 nucleotides ova and embryos contain in different stages of development. (helsinki.fi)
For the first time, we have identified short non-coding RNA molecules in ova at different stages of maturity, in fertilised ova and in early embryos with the help of sequencing, as well as determined their editing on the molecular level in embryos. (helsinki.fi)
Small RNA expression and miRNA modification dynamics in human oocytes and early embryos. (helsinki.fi)
In 1-cell and 2-cell embryos Dnmt1s is derived from the oocyte, whereas from the 2-cell stage onward the embryo starts to synthesize its own Dnmt1s 8 . (nature.com)
The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)
50 miles, previous live birth, fewer oocytes retrieved, and not having embryos cryopreserved were also associated with higher rates of discontinuation. (cdc.gov)

Das D, Arur S . Regulation of oocyte maturation: Role for ERK Signaling. (mdanderson.org)
To determine if the effect of TGF-beta1 on oocyte maturation involves transcription and/or translation, ovarian follicles were pre-treated with actinomycin D, a blocker of transcription, and cyclohexamide, an inhibitor of translation, and incubated with hCG or DHP, either alone or in combination with TGF-beta1 and oocyte maturation scored. (biomedcentral.com)
Treatment with actinomycin D, a blocker of transcription, reduced the inhibitory effect of TGF-beta1 on DHP-induced oocyte maturation, indicating that the inhibitory action of TGF-beta1 is in part due to regulation of gene transcription. (biomedcentral.com)
These findings suggest that TGF-beta1 acts at multiple sites, including LHR, 20beta-HSD and mPR-beta, to inhibit zebrafish oocyte maturation. (biomedcentral.com)
Oocyte maturation in teleosts is triggered by the release of leutinizing hormone (LH) from the pituitary. (biomedcentral.com)
1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)

Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)
We quantified 7277 genes expressed in the cumulus-oocyte complex. (auburn.edu)
Independent clustering of co-expressed genes revealed critical biological functions for the oocyte, such as regulation of transcription, stem cell population maintenance, and insulin receptor signaling pathway. (auburn.edu)
The sequencing of single oocytes and their surrounding somatic cells revealed coordinated expression of hundreds of genes that formed functional regulatory networks. (auburn.edu)
Here, we report the genomic organization and evolution of the three il1rl1 genes (il1rl1 genes 1-3), and use both RNA-seq and gene-specific qPCR methods to quantify expression patterns. (usda.gov)
Analysis of transcript abundance in 15 tissues obtained from healthy adult rainbow trout by RNA-seq indicates constitutive expression of all three genes with the highest relative expression of gene-1 in oocyte tissue and highest expression of gene-2 and -3 in gill tissue. (usda.gov)
Mitochondrial genes are typically inherited only from the maternal oocyte. (msdmanuals.com)
In addition, mitochondrial DNA (mtDNA) encodes 2 ribosomal RNA genes and 22 transfer RNA (tRNA) genes necessary for the intramitochondrial synthesis of these 13 polypeptides. (medscape.com)
The proposed mechanism is based on the knowledge that transcription of mtDNA is polycistronic, which means that all genes encoded on the heavy and light strands are transcribed as 2 large precursor RNA strands. (medscape.com)

The oocyte is loaded with maternal mRNAs and proteins that control the cell maintenance and fate and the formation of the body plan prior to the onset of zygotic genome expression [ 3 , 4 ]. (biomedcentral.com)
Mitochondria contain DNA in a single circular chromosome that codes for 13 proteins, various RNAs, and several regulating enzymes. (msdmanuals.com)

GABA responses expressed by bovine retina poly(A)+ RNA also were Cl- currents but were composed of two pharmacologically distinct components, one mediated by GABAA receptors and the other by GABA receptors with novel properties, which were resistant to bicuculline but were not activated by R(+)-baclofen, a selective agonist of GABAB receptors. (aspetjournals.org)
Moreover, we use bovine and porcine oocytes as model systems for aspects of meiosis that are not well conserved in the mouse. (uni-goettingen.de)
We profiled the total RNA of single oocytes (bovine and porcine), and observed distinct peaks for small RNAs, 18S, and 28S. (auburn.edu)

These data suggest that FECR1 circular RNA acts as an upstream regulator to control breast cancer tumor growth by coordinating the regulation of DNA methylating and demethylating enzymes. (biomedcentral.com)
Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)

In addition, we are studying selected aspects of meiosis in human oocytes. (uni-goettingen.de)
Mechanism of spindle pole organization and instability in human oocytes. (uni-goettingen.de)

Trimmer KA, Zhao P, Seemann J, Chen SY, Mondal S, Ben-Yakar A and Arur S. Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma. (mdanderson.org)
Aneuploidy in mammalian oocytes and the impact of maternal ageing. (uni-goettingen.de)

A couple of days from fertilisation, when the embryo consists of four cells, the embryonic genome is already active, producing for the first time several RNA molecules. (helsinki.fi)
As the fertilised ovum develops and the genome of the embryo is activated, the number of oocyte-specific piRNA molecules drops dramatically and the share of the better known micro-RNA (miRNA) molecules in the embryo increases. (helsinki.fi)

As reported in neurons and in other expression systems, GABAA responses expressed in oocytes by cerebral cortex RNA were strongly and stereospecifically potentiated by 5 alpha-pregnan-3 alpha-ol-20-one (3 alpha-OH-DHP) and 5 alpha-pregnan-3 alpha,21-diol-20-one (THDOC). (aspetjournals.org)
The objective of this study was to generate tissue and cell-type expression profiles in order to verify the oocyte-specificity of these novel lncRNAs as validation for selection for experimentation. (sare.org)
Strand-specific, massively parallel cDNA sequencing (RNA-seq) is a powerful tool for transcript discovery, genome annotation and expression profiling. (nature.com)
Figure 6: Digital expression profiling using strand-specific RNA-seq. (nature.com)
These results indicate that the zygotic expression pattern is similar in frogs and fish, and that there is a conserved zygotic expression of Vg1 RBP distinct from its expression in the oocyte. (zfin.org)

iii) we develop new tools to study meiosis in mammalian oocytes in order to open new venues for research for the Department and for the meiosis community. (uni-goettingen.de)
Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment. (uni-goettingen.de)
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. (uni-goettingen.de)

GABA responses expressed by rat cerebral cortex poly(A)+ RNA were bicuculline-sensitive Cl- currents mediated by GABAA receptors. (aspetjournals.org)

A 4-thio-rU modified RNA pentamer was used to study the effect of this modification on codon-anticodon interaction when it is in the wobble position of tRNA. (fishersci.com)
4-thio-rU modified RNA oligos have been used as photoaffinity probes in the role of substrate analogs for characterizing the enzyme:substrate complex of tRNA:pseudouridine-5S synthase. (fishersci.com)

Weighing each method's performance and ease, we identified the dUTP second-strand marking and the Illumina RNA ligation methods as the leading protocols, with the former benefitting from the current availability of paired-end sequencing. (nature.com)
4-thio-rU modified oligos have been used as modules for assembling U25 small nucleolar RNAs (U25snoRNA) by ligation. (fishersci.com)

Vg1 RBP (protein) colocalizes with Vg1 RNA at all stages of oogenesis. (zfin.org)
Vg1 RBP RNA, however, localizes to the animal pole during late oogenesis, and remains in the animal blastomeres and ectodermal precursors until its zygotic transcription is activated, around stage 12. (zfin.org)
In situ hybridization reveals that Vg1 RBP RNA localizes early in oogenesis to the animal pole. (zfin.org)

Analyses of single oocytes are essential for further clarification of these molecular mechanisms. (auburn.edu)

The non-coding genome produces RNA molecules, which regulate the function of the entire genome. (helsinki.fi)
The group active at the University of Helsinki has now surveyed the short non-coding RNA molecules which regulate the functioning of the embryonic genome. (helsinki.fi)
Das D, Chen SY and Arur S *. ERK phosphorylates chromosomal axis component HORMA domain protein HTP-1 to regulate oocyte numbers. (mdanderson.org)

Standard protocols to obtain RNA for single-cell RNA-Seq involve RNA extraction kits that select for mRNAs or cell lysis procedures, which present limitations when the oocyte is the cell of interest. (auburn.edu)

Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and transcription cis-regulatory region binding activity. (jax.org)
Reduced female fertility due to sequestration of RNA Pol II by pervasive transcription in exosome RNase-depleted oocytes. (nih.gov)

Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. (nature.com)

Injection of cloned RNA into oocytes increased Na+/sugar cotransport by three orders of magnitude. (xenbase.org)

We hypothesized that the transcript profiles of the cumulus cells and oocyte display distinct gene regulatory networks within the oocyte and cumulus cells. (auburn.edu)

We applied this approach to sequence the transcriptome of 17 prepubertal porcine oocytes and their corresponding cumulus cells. (auburn.edu)

The oocyte acquires developmental competence as it progresses through folliculogenesis. (auburn.edu)

In addition to potentiation, 3 alpha-OH-DHP and THDOC caused pronounced increases in the rate of desensitization of GABAA responses expressed by cortex RNA. (aspetjournals.org)

The work in our laboratory combines several innovative approaches towards understanding the fundamental principles underlying meiosis, including advanced microscopy techniques (such as super-resolution, light sheet, expansion, and focused ion beam electron microscopy), loss-of-function approaches (Trim-Away, RNAi in cultured follicles, and mouse genetics), as well as in vitro ovarian culture and sequencing (including single-cell RNA-seq). (uni-goettingen.de)

Morphological descriptions of RNA granules originated with Metschnikoff (1865) , who described dark staining granules at one pole within Miastor metraloas (fly) larvae. (rupress.org)
Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)

We review the relationship between different classes of these granules and discuss how spatial organization regulates messenger RNA translation/decay. (rupress.org)

However, no correlation were categorised into 2 groups: 73 infertile has been found between these antibodies women presenting for an infertility evalu- and the number of oocytes and pregnancy ation, with a mean duration of infertility rate [ 6 ]. (who.int)

The researchers found that most of the short RNA molecules in human ova belong to a class of molecules that was identified only recently in the ova of humans and primates. (helsinki.fi)
These oocyte-specific piwi-interacting RNA (piRNA) molecules, or RNA molecules originating in the ovum, are not found in mice, which are used as the model animal," Vuoristo says. (helsinki.fi)

This is not necessarily a problem for the cell or tissue because multiple copies of mtDNA are present in each cell (in oocytes, this is in the region of 100,000 copies per cell), and both wild type and mutated mtDNA can coexist, a situation known as heteroplasmy. (medscape.com)

microRNAs (miRNAs) have been emerged as a class of novel noncoding RNA regulator, and their length are approximately 22 nucleotides in length [ 4 ]. (hindawi.com)

Thus, FLI1 drives tumor metastasis not only through the canonical oncoprotein pathway, but also by using epigenetic mechanisms mediated by its exonic circular RNA. (biomedcentral.com)

Description: RNA profiling data sets generated by the Mouse ENCODE project. (nih.gov)
Abce1 orchestrates M-phase entry and cytoskeleton architecture in mouse oocyte. (nih.gov)
Most of our work is carried out in mouse oocytes, which are easily available and can be studied with state-of-the-art molecular biology, genetic and advanced imaging tools. (uni-goettingen.de)

At the end of each experiment, total RNA was extracted and reverse transcribed. (biomedcentral.com)

There are multiple published methods for strand-specific RNA-seq, but no consensus exists as to how to choose between them. (nature.com)
Figure 1: Methods for strand-specific RNA-seq. (nature.com)

Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
Using this CasIP assay, we identified FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter and epigenetically activates FLI1 in breast cancer cells. (biomedcentral.com)

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