Oocytes. Medical search. Frequent questions

Many studies show that cumulus expansion is critical for the maturation of the oocyte because the cumulus complex is the oocyte's direct communication with the developing follicle environment. (wikipedia.org)
since all oocytes produced during this time are of equivalent "depth", the production-line hypothesis of postnatal oocyte maturation cannot logically explain increasing oocyte depth as females age. (science20.com)
We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. (springer.com)
Galvão A, Segers I, Smitz J, Tournaye H, De Vos M. In vitro maturation (IVM) of oocytes in patients with resistant ovary syndrome and in patients with repeated deficient oocyte maturation. (springer.com)
After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
We previously found that mutations in TUBB8 caused oocyte maturation arrest. (bmj.com)
Methods and results Patients with oocyte maturation arrest were sequenced with respect to TUBB8 . (bmj.com)
In experiment III, worms collected in spring were exposed to different temperature regimes to examine the effect of raising temperature on the synchronization of oocyte growth and maturation. (frontiersin.org)
The results showed worms collected in spring showed increased temperatures will increase oocyte maturation synchronicity. (frontiersin.org)
The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
T mice, this can be explained by the preferential propagation of mtDNA during oocyte maturation, counterbalanced by purifying selection against high heteroplasmy levels. (cam.ac.uk)
van den Hurk R, Zhao J. Formation of mammalian oocytes and their growth, differentiation and maturation within ovarian follicles. (harvard.edu)
To better understand this control, we profiled translational efficiencies and poly(A)-tail lengths throughout Drosophila oocyte maturation and early embryonic development. (elifesciences.org)
Our results also revealed the presence of tail-length-independent mechanisms that maintained translation despite tail-length shortening during oocyte maturation, and prevented essentially all translation of bicoid and several other mRNAs before egg activation. (elifesciences.org)
The production of viable offspring requires three key developmental events: oocyte maturation, the oocyte-to-embryo transition (OET), and the maternal-to-zygotic transition (MZT) ( Figure 1A ). (elifesciences.org)
Oocyte maturation involves the release of the primary meiotic arrest at prophase I and progression of the oocyte nucleus into meiotic divisions to produce a mature oocyte (egg) capable of being fertilized ( Von Stetina and Orr-Weaver, 2011 ). (elifesciences.org)
Roy SR, Wang J, Rana MR, Nakashima M, Tokumoto T. Characterization of membrane progestin receptor α (mPRα) of the medaka and role in the induction of oocyte maturation. (gene-tools.com)
Potential supplementary approaches to fertility preservation include retrieval of immature oocytes followed by in vitro maturation (IVM) and storage. (bvsalud.org)
1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)

The oocyte, a large and complex cell, must be supplied with numerous molecules that will direct the growth of the embryo and control cellular activities. (wikipedia.org)
Background TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. (bmj.com)
Enhanced water recipe and enrichment for oocyte quality and embryo development in the African clawed frog (Xenopus laevis). (awionline.org)
Such a method of live-cell lipid quantification has (i) experimental power in basic cell biology, (ii) practical utility for identifying developmental predictive biomarkers while advancing biology-based oocyte/embryo selection, and (iii) ability to yield rationally supporting technology for decision-making in rodents, domestic species, and human assisted reproduction and/or fertility preservation. (rsc.org)
Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)
There is insufficient evidence to recommend a particular stimulation protocol for oocyte donors or certain donor characteristics in terms of embryo quality or success rates. (guidelinecentral.com)
11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
When a normal sperm cell fertilizes one of these oocytes, the resulting embryo has only one set of chromosomes. (medlineplus.gov)
The health of the mature oocyte and subsequent embryo is highly dependent on the oocyte mitochondria. (cdc.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
For assisted reproductive techniques, oocytes and sperm are collected from the intended parents or donors, and an embryo or the gametes are transferred to the woman's reproductive tract after culture in vitro. (msdmanuals.com)

The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. (springer.com)
Because maturing oocytes and early embryos lack appreciable transcription, posttranscriptional regulatory processes control their development. (elifesciences.org)
However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. (life-science-alliance.org)
We herein report that oocyte-specific Uhrf1 KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. (life-science-alliance.org)
Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
Experimental spindle transfer in metaphase II oocytes, also called mitochondrial replacement therapy, is a novel technology for preventing mtDNA transmission from oocytes to pre-implantation embryos. (infertile.com)
The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)
All embryos and oocytes that were frozen and thawed were included in the study. (bvsalud.org)
Although a relatively high number of mature oocytes and embryos can be stored with the combined procedure , the limited rate of pregnancies represents a poor reproductive outcome. (bvsalud.org)

The oocyte is arrested in Meiosis II at the stage of metaphase II and is considered a secondary oocyte. (wikipedia.org)
These disturbances may lead ultimately to errors in chromosome segregation during the metaphase of meiosis, and that faulty segregation to oocyte aneuploidy. (cea.fr)
The mature oocyte is arrested in metaphase II in most vertebrates and metaphase I in insects. (elifesciences.org)
Spindle transfer from the patient's metaphase II oocyte into the cytoplasm of a donor oocyte. (infertile.com)

In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)
Oocyte cryopreservation (egg-freezing), Kallen explained, is when a woman's eggs are removed from the ovaries and stored for future use as "a way of preserving reproductive ability in women (or individuals with ovaries)" and an alternative for "anyone who isn't planning pregnancy immediately" but might want to explore their options in the future. (uvm.edu)
Evidence-based outcomes after oocyte cryopreservation for donor oocyte in vitro fertilization and planned oocyte cryopreservation: a guideline. (guidelinecentral.com)
Differences in perceived stress during ovarian stimulation between women with infertility and those pursing oocyte cryopreservation. (uchicago.edu)
We reviewed the records of 119 cancer patients who underwent ovarian tissue cryopreservation and immature oocyte harvesting for fertility preservation . (bvsalud.org)
We were sending young women to him for oocyte or ovarian cryopreservation back in the early 90s. (medscape.com)

During oogenesis, the oogonia become primary oocytes. (wikipedia.org)
The formation of an oocyte is called oocytogenesis, which is a part of oogenesis. (wikipedia.org)
Oogenesis results in the formation of both primary oocytes during fetal period, and of secondary oocytes after it as part of ovulation. (wikipedia.org)
During the primary oocyte stage of oogenesis, the nucleus is called a germinal vesicle. (wikipedia.org)
Postnatal oogenesis through ongoing oogonial stem cell (OSC) mitosis explains increasing oocyte depth with age. (science20.com)
It was the first to be shown to alter oogenesis and lead to the formation of oocytes with an abnormal number of chromosomes (aneuploidy), in turn responsible for numerous miscarriages and fertility disorders. (cea.fr)
Three different experiments were conducted to examine the oocyte development of the polychaete Hediste diversicolor in response to changing photoperiod and temperature at three different periods of oogenesis. (frontiersin.org)

On Thursday, Oct. 12, Associate Professor in the Department of Molecular Biosciences at Northwestern University Sadie Wignall shared her findings on the mechanisms oocytes employ to regulate spindles without centrosomes during meiosis. (jhunewsletter.com)
The seminar , titled "Stabilizing a Dynamic Structure: Mechanisms that Maintain Acentrosomal Spindle Integrity During Oocyte Meiosis," was hosted by the Department of Biology at Hopkins for their seminar series. (jhunewsletter.com)
During her postdoctoral fellowship, Wignall began studying meiosis - a type of cell division that produces reproductive cells with half the number of chromosomes - occurring in female reproductive cells or oocytes. (jhunewsletter.com)
Conclusions Our data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8 , underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF). (bmj.com)
In most animals the oocyte is arrested in meiosis, and fertilization leads to initiation of mitosis as the oocyte nucleus completes meiosis and fuses with the haploid sperm nucleus. (elifesciences.org)
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. (uchicago.edu)
The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). (uchicago.edu)
nondisjunction -- a failure of proper homolog separation in meiosis I, or sister chromatid separation in meiosis II can lead to aneuploidy , in which the oocyte has the wrong number of chromosomes, for example 22,X or 24,X. This is the cause of conditions like Down syndrome and Edwards syndrome . (wikidoc.org)

The notion of a biological clock in women arises from the fact that immature egg cells- 'oocytes' - progressively decline in number as females get older, along with a decades-old view that oocytes cannot be renewed in mammals after birth. (science20.com)
Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
After oocyte collection, immature oocytes were cultured in the IVM medium. (springer.com)
Outcome of immature oocytes collection of 119 cancer patients during ovarian tissue harvesting for fertility preservation. (bvsalud.org)
The aim of this study was to evaluate post-thawing outcomes of immature oocytes collected both by transvaginal aspiration and from excised ovarian tissue . (bvsalud.org)

Preprint Highlight: Age-dependent loss of cohesion protection in human oocytes. (iisertirupati.ac.in)

On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
The generation of a zygote through fertilization marks the onset of development for the offspring, and requires the proper completion of sperm and oocyte development in the respective parents. (elifesciences.org)
This study taking to account the limitations of studies in this area, aimed to compare the survival rate, fertilization capacity and developmental capacity of oocyte that cryo-preserved in open pulled and conventional straw. (scialert.net)
Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)

The only normal human type of secondary oocyte has the 23rd (sex) chromosome as 23,X (female-determining), whereas sperm can have 23,X (female-determining) or 23,Y (male-determining). (wikipedia.org)
The oocytes (eggs) are removed from the donor and fertilized with sperm in the laboratory, then transferred to the recipient. (bcm.edu)
Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). (springer.com)
Then sperm were added to living control, cryo-preserved in conventional and open pulled straws oocytes. (scialert.net)
Oocytes may need to be cryo-stored in the event of unforeseen non-production of sperm during IVF therapy, allowing a more measured consideration of donor sperm use or other means of sperm retrieval. (scialert.net)
Mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction. (oregonstate.edu)
Morphometric and morphokinetic differences in the sperm- and oocyte-or" by Lee-Sarose Orevich, Kate Watson et al. (edu.au)
All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by one embryologist (L-SO). (edu.au)
Timing parameters included 2nd polar body extrusion (tPB2), sperm-originated PN (tSPNa) or oocyte-originated PN (tOPNa) appearance, and PN fading (tPNF). (edu.au)
The only normal type of secondary oocyte has sex chromosomes 23,X (where sperm can be 23,X or 23,Y). (wikidoc.org)
A small number of cases of recurrent hydatidiform mole have been found to be caused by mutations in genes that play important roles in the production of oocytes and sperm cells. (medlineplus.gov)
during oocyte and sperm cell production. (medlineplus.gov)
The semen sample is typically washed several times with tissue culture medium and is concentrated for motile sperm, which are then added to the medium containing the oocytes. (msdmanuals.com)
At this point, intracytoplasmic sperm injection-injection of a single sperm into each oocyte-may be done, particularly if spermatogenesis is abnormal in the male partner. (msdmanuals.com)
After sperm are added, the oocytes are cultured for about 2 to 5 days. (msdmanuals.com)
4. Use of sperm or oocyte donors. (who.int)

British Library EThOS: The use of Xenopus laevis oocytes as a transcription assay. (bl.uk)
Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and retina. (aspetjournals.org)
Electrical recordings were made in Xenopus oocytes to study the modulatory effects of steroids on gamma-aminobutyric acid (GABA) receptors expressed by RNA from mammalian brain and retina. (aspetjournals.org)

The oocyte receives mitochondria from maternal cells, which will go on to control embryonic metabolism and apoptotic events. (wikipedia.org)
The partitioning of mitochondria is carried out by a system of microtubules that will localize mitochondria throughout the oocyte. (wikipedia.org)
In certain organisms, such as mammals, paternal mitochondria brought to the oocyte by the spermatozoon are degraded through the attachment of ubiquitinated proteins. (wikipedia.org)
Then, having established the full complement of mitochondria in the fully grown oocyte, there is no further increase of the mitochondrial population during early development. (nih.gov)
Toxicant effects on mammalian oocyte mitochondria. (cdc.gov)
Oocyte mitochondria are unique organelles that establish a founder population in primordial germ cells (PGCs). (cdc.gov)
As the oocyte matures in the postnatal mammalian ovary during folliculogenesis it increases exponentially in volume, and the oocyte mitochondria population proliferates to about 100 000 mitochondria per healthy, mature murine oocyte. (cdc.gov)
Here we review the effects that toxic insults including chemotherapeutics, toxic metals, plasticizers, pesticides , polycyclic aromatic hydrocarbons (PAHs), and ionizing radiation can have on oocyte mitochondria. (cdc.gov)
This is very clearly a burgeoning field, as our understanding of oocyte mitochondria and metabolism is still relatively new, and we contend much more research is needed to understand the detrimental impacts of exposure to toxicants on oocyte mitochondria. (cdc.gov)

An oocyte (UK: /ˈoʊəsaɪt/, US: /ˈoʊoʊ-/), oöcyte, or ovocyte is a female gametocyte or germ cell involved in reproduction. (wikipedia.org)

An oocyte is produced in a female fetus in the ovary during female gametogenesis. (wikipedia.org)
An oocyte is part the ovary development. (wikidoc.org)
After the onset of puberty and menses, the female human ovary recruits at least 30-50 oocytes during each menstrual cycle. (medscape.com)

Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
The decline in the number of oocytes begins at 20 weeks' gestation when the female fetus has approximately 6-7 million oogonia (largest lifetime endowment). (medscape.com)

This stage the oogonia is now an Oocyte (pronounced oh'a (like Noah)-site ). (wikidoc.org)

Oocytes are rich in cytoplasm, which contains yolk granules to nourish the cell early in development. (wikipedia.org)
These mRNAs can be stored in mRNP (message ribonucleoprotein) complexes and be translated at specific time points, they can be localized within a specific region of the cytoplasm, or they can be homogeneously dispersed within the cytoplasm of the entire oocyte. (wikipedia.org)
G), with a long history of multiple undiagnosed pregnancy losses and deaths of offspring as a result of this disease, who underwent IVF after reconstitution of her oocytes by spindle transfer into the cytoplasm of enucleated donor oocytes. (infertile.com)
Oocytes of Rana pipiens were incubated in 22 Na-Ringer's solution and 22 Na ‐ 23 Na exchange in the nucleus and cytoplasm followed by low-temperature microdissection. (biologists.com)
In an earlier paper, we described a low-temperature microdissection technique and its application to the determination of sodium, potassium, and water in the nucleus and cytoplasm of the mature amphibian oocyte ( Century, Fenichel & Horowitz, 1970 ). (biologists.com)
In this study, we used the low-temperature microdissection technique to investigate the 22 Na exchange in the nucleus and cytoplasm of Rana pipiens oocytes incubated in 22 Na-Ringer's solution. (biologists.com)

While traditional thinking has held that female mammals are born with all of the eggs they will ever have, newer research has demonstrated that adult mouse and human ovaries contain a rare population of progenitor germ cells called oogonial stem cells capable of dividing and generating new oocytes. (science20.com)
Researchers traced the number of divisions a cell has undergone with age (its 'depth') and counted the number of times progenitor germ cells divided before becoming oocytes. (science20.com)
In a new assessment of the work by Shapiro and colleagues, reproductive biologists Dori Woods, Evelyn Telfer and Jonathan Tilly conclude that the most plausible explanation for these findings is that progenitor germ cells in ovaries continue to divide throughout reproductive life, resulting in production of new oocytes with greater depth as animals age. (science20.com)
In a work published in Environmental Pollution , researchers from iRCM's LDG laboratory explored the effects of exposure to two bisphenol A substitutes, BADGE and BPAF, on murine germ cells, and more specifically on oocytes and their precursors. (cea.fr)

As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. (lu.se)
Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). (lu.se)

The oocyte donor may be anonymous and obtained through a donor egg agency, or, if desired, the donor can be designated by the patient (a friend or a sibling or other female relative with similar genetic makeup). (bcm.edu)
The procedure can be undertaken at virtually any time because the rate limiting issue is the age of the oocyte donor. (bcm.edu)
Heritable genetic diseases can be prevented with the use of donor oocytes. (bmj.com)
We report our experience in using donor oocytes from anonymous, matched, fertile donors in four women with heritable genetic disorders. (bmj.com)
Our results show that use of donor oocytes is a practical, successful, and currently available technique for the prevention of genetic disorders. (bmj.com)
D) transfer of the removed spindle into the perivitelline space of the enucleated donor oocyte. (infertile.com)
It is recommended to tell recipients that previously cryopreserved donor oocytes are a reasonable option compared with fresh donor oocytes, given that there is good evidence that there are no significant differences in per transfer pregnancy rates compared with those with fresh donor oocytes. (guidelinecentral.com)
Recipients can be counseled that as the number of donor oocytes warmed increases, there is an associated increase in cumulative live birth rate. (guidelinecentral.com)
Recipients can be counseled that, based on limited evidence, neonatal outcomes appear similar between vitrified and fresh donor oocytes. (guidelinecentral.com)
There are no significant differences in per transfer pregnancy rates with cryopreserved vs. fresh donor oocytes. (guidelinecentral.com)
Title : Trends and Outcomes for Donor Oocyte Cycles in the United States, 2000-2010 Personal Author(s) : Kawwass, Jennifer F.;Monsour, Michael;Crawford, Sara;Kissin, Dmitry M.;Session, Donna R.;Kulkarni, Aniket D.;Jamieson, Denise J. (cdc.gov)

The proteins produced from the NLRP7 and KHDC3L genes are critical for normal egg cell (oocyte) development, which impacts embryonic development. (medlineplus.gov)

Without gonadotropin priming and HCG trigger, ultrasound-guided transvaginal oocyte retrieval was performed. (springer.com)
Objective: To assess the impact of class III obesity on outcomes and complications of transvaginal ultrasound-guided oocyte pickup (OPU). (bepress.com)

Egg retrieval (also called oocyte retrieval). (cdc.gov)
pregnancy rates with this technique are lower than those with retrieval of multiple oocytes, but costs are lower and success rates are increasing. (msdmanuals.com)

The cumulus-oocyte complex contains layers of tightly packed cumulus cells surrounding the oocyte in the Graafian follicle. (wikipedia.org)
We applied this approach to sequence the transcriptome of 17 prepubertal porcine oocytes and their corresponding cumulus cells. (auburn.edu)
We hypothesized that the transcript profiles of the cumulus cells and oocyte display distinct gene regulatory networks within the oocyte and cumulus cells. (auburn.edu)
We quantified 7277 genes expressed in the cumulus-oocyte complex. (auburn.edu)

In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. (lu.se)
A pregnancy that results from an abnormal oocyte cannot develop properly, resulting in recurrent hydatidiform mole. (medlineplus.gov)
In affected women, this can lead to the production of abnormal oocytes that do not contain chromosomes. (medlineplus.gov)

A female reproductive cell, also called an oocyte or ovum. (cdc.gov)

Woods and colleagues say that "the recent work of Shapiro and colleagues is one of the first reports to offer experimental data consistent with a role for postnatal oocyte renewal in contributing to the reserve of ovarian follicles available for use in adult females as they age. (science20.com)

Infertile women should be counseled that, based on a small number of births, neonatal outcomes appear similar after using their own previously cryopreserved oocytes compared with outcomes after the use of fresh oocytes. (guidelinecentral.com)
Neonatal outcomes appear similar with cryopreserved oocytes. (guidelinecentral.com)

An oocyte is a form of genetic material that can be collected for cryoconservation. (wikipedia.org)
Oocyte donation is also an option for those who are carriers of genetic diseases and wish to prevent the risk of either conceiving a child affected with the disease or who might be a carrier of a genetic disease. (bcm.edu)
A recent genetic study tracing the origins of oocytes from the embryonic period throughout adulthood and adds new information to a growing controversy. (science20.com)
The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
Via genetic and chemical models, the team was able to propose that the deleterious effects of bisphenols on mature oocytes results from their ability to induce oxidative lesions on DNA at a key step in germinal cell differentiation during the fetal period. (cea.fr)
Use of oocytes from anonymous, matched, fertile donors for prevention of heritable genetic diseases. (bmj.com)
However, it is also possible that such genetic defects result in a smaller complement of oocytes during gonadal development. (ldysinger.com)

b) If continued proliferation of OSCs (red) and their subsequent differentiation into oocytes (blue) during postnatal life is superimposed on the production-line hypothesis, the emerging picture is consistent with a progressive increase in oocyte depth in females as they age. (science20.com)

As the oocyte is a product of female gametogenesis, the maternal contribution to the oocyte and consequently the newly fertilized egg, is enormous. (wikipedia.org)
It was proposed that, in order to avoid damage to the DNA genome of the oocytes, the metabolism contributing to the synthesis of much of the oocyte's constituents was shifted to other maternal cells that then transferred these constituents to oocytes. (wikipedia.org)
During the growth of the oocyte, a variety of maternally transcribed messenger RNAs, or mRNAs, are supplied by maternal cells. (wikipedia.org)
The oocyte is loaded with maternal mRNAs and proteins that control the cell maintenance and fate and the formation of the body plan prior to the onset of zygotic genome expression [ 3 , 4 ]. (biomedcentral.com)

Although these investigations were performed in mice, there is emerging evidence that oogonial stem cells are also present in the ovaries of reproductive-age women, and these cells possess the capacity, like their mouse counterparts, to generate new oocytes under certain experimental conditions. (science20.com)

The number and quality of a woman's oocytes declines with age. (medscape.com)

With oocyte donation, any woman with a healthy uterus, regardless of the condition of her own eggs, can potentially bear a child with her partner. (bcm.edu)
Oocyte donation is proven, well-established technology with which there is considerable expertise. (bcm.edu)
Recipients can be counseled that live birth rates may be greater from vitrified oocytes that derive from donors who had prior successful outcome after a fresh cycle of oocyte donation. (guidelinecentral.com)

These mutations cause a range of folding defects in vitro, different degrees of microtubule disruption upon expression in cultured cells and interfere to varying extents in the proper assembly of the meiotic spindle in mouse oocytes. (bmj.com)

Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism, signal perception and transduction, RNA processing, cell cycle, defense against pathogens and DNA damage. (biomedcentral.com)

In its study performed in a mouse model and published in Environmental Pollution , the team showed that fetal exposure to environmental concentrations of BADGE or BPAF caused oocyte defects in mature mice comparable to those provoked by fetal BPA exposure . (cea.fr)

Oocytes without a zona pellucida and up to one layer of flattened granulosa progenitor cells were classified as primordial follicles. (harvard.edu)
Since it is formed at an early stage in women harbouring ASA and AZP-Ab in of oocyte growth, zona pellucida-specific their cervical secretions. (who.int)

The progressive loss of oocytes from fetal life through menopause is a normal process. (ldysinger.com)

Their results show that oxidative damage caused by the substitutes has important consequences on oocyte quality. (cea.fr)

Below are some examples of maternally inherited mRNAs and proteins found in the oocytes of the African clawed frog. (wikipedia.org)
A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. (life-science-alliance.org)
Within oocytes, the exact role of NLRP7 and KHDC3L proteins are not known. (medlineplus.gov)

Prior to the onset of menopause (10-15 y), menstrual cycles shorten and rapid follicular loss occurs because more oocytes are being recruited. (medscape.com)

We profiled the total RNA of single oocytes (bovine and porcine), and observed distinct peaks for small RNAs, 18S, and 28S. (auburn.edu)

We investigated the effects of identified mutations in vitro, in cultured cells and in mouse oocytes. (bmj.com)
Primary follicles were classified as oocytes surrounded by one layer of cuboidal granulosa cells. (harvard.edu)
Oocytes with two or more layers of granulosa cells but no visible space between granulosa cells were identified as preantral follicles. (harvard.edu)
The sequencing of single oocytes and their surrounding somatic cells revealed coordinated expression of hundreds of genes that formed functional regulatory networks. (auburn.edu)
Regulation of Insulin-Like Growth Factor 2 by Oocyte-Secreted Factors in Primary Human Granulosa Cells. (uchicago.edu)

Standard protocols to obtain RNA for single-cell RNA-Seq involve RNA extraction kits that select for mRNAs or cell lysis procedures, which present limitations when the oocyte is the cell of interest. (auburn.edu)

developmental potential of buffalo oocytes vitrified at the germinal vesicle stage: effects of different cryoprotectant combinations and cryodevices, , 2016. (cu.edu.eg)

The oocyte acquires developmental competence as it progresses through folliculogenesis. (auburn.edu)

There are many types of molecules that are maternally supplied to the oocyte, which will direct various activities within the growing zygote. (wikipedia.org)
Thus, oocytes of many organisms are protected from oxidative DNA damage while storing up a large mass of substances to nurture the zygote in its initial embryonic growth. (wikipedia.org)

Independent clustering of co-expressed genes revealed critical biological functions for the oocyte, such as regulation of transcription, stem cell population maintenance, and insulin receptor signaling pathway. (auburn.edu)

Mitochondrial DNA heteroplasmy is modulated during oocyte development propagating mutation transmission. (cam.ac.uk)
A male euploid blastocyst was obtained from the reconstituted oocytes, which had only a 5.7% mtDNA mutation load. (infertile.com)

Recipients can be counseled that the length of time that oocytes have been stored is not associated with differences in oocyte survival or pregnancy rates. (guidelinecentral.com)
However, no correlation were categorised into 2 groups: 73 infertile has been found between these antibodies women presenting for an infertility evalu- and the number of oocytes and pregnancy ation, with a mean duration of infertility rate [ 6 ]. (who.int)

Results of this study demonstrate that CARS is able to identify lipids in live mammalian oocytes, and there exists quantifiable and consistent differences in percent lipid composition across ooctyes of different species, developmental stages, and in relation to body composition. (rsc.org)
One of those problems is the freezing solution volume that the oocyte is immersed into and the other is too thick wall straw that this thickness can affect the heat exchange while oocyte freezing and thawing and the results can affect ice crystals formed. (scialert.net)
Our results show the model proposed for Eurycea oocytes is also applicable to those of Rana and probably to amphibian oocytes in general. (biologists.com)

Anatomy37

Organisms18

Diseases8

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Psychiatry and Psychology1

Phenomena and Processes64

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science4

Health Care2

Maturation23

Embryo11

Embryos10

Metaphase4

Cryopreservation6

Oogenesis7

Meiosis8

Immature5

Human oocytes1

Fertilization4

Sperm16

Xenopus3

Mitochondria9

Ovocyte1

Ovary3

Decline in the Number of Oocytes2

Oogonia1

Cytoplasm6

Germ4

Quality of oocytes1

Drosophila2

Donor11

Embryonic development1

Transvaginal2

Retrieval2

Cumulus4

Abnormal3

Ovum1

Ovarian follicles1

Outcomes2

Genetic7

Differentiation1

Maternal4

Reproductive1

Woman's1

Donation3

Meiotic spindle1

Metabolism1

Defects1

Zona2

Fetal1

Oxidative Damage1

Proteins3

Cycles1

Bovine1

Cells5

MRNAs1

Germinal1

Developmental competence1

Zygote2

Genes1

Mutation2

Pregnancy2

Results3