AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
VitrificationEthylene GlycolCryopreservationOocytesCryoprotective AgentsFertilization in VitroOogenesisOvarian FollicleBlastocystPropylene GlycolEmbryo Culture TechniquesFreezingEmbryo TransferEmbryonic DevelopmentTissue PreservationFertility PreservationMorulaOocyte DonationOocyte RetrievalDimethyl SulfoxideFreeze SubstitutionThermal ConductivityIn Vitro Oocyte Maturation TechniquesCleavage Stage, OvumEmbryo, MammalianPropylene GlycolsReproductive Techniques, AssistedPregnancy RatePregnancyOvaryTissue SurvivalEthylene GlycolsEmbryo ImplantationMetaphaseCumulus CellsFertilizationMeiosisTrehaloseSperm Injections, IntracytoplasmicPregnancy OutcomeParthenogenesisXenopus laevisAsepsisRewarmingZona PellucidaMice, Inbred ICRCell SurvivalZygoteIceDry IceTreatment OutcomeEctogenesisHEPESSucroseLive BirthTime FactorsTissue Culture TechniquesXenopusButylene GlycolsSus scrofaFetal ViabilityEmbryonic and Fetal DevelopmentOvulation InductionBlastomeresOutcome Assessment (Health Care)Single Embryo TransferMicroinjectionsSpermatozoaCattleSolutionsFormamides
CryopreservationEmbryosFertility preservationDonationRetrievalImprove outcomesICSIDonorNeonatalCyclesMatured in vitroSurvivalClinical outcomesPerinatal outcomesFetal lifeRetrospectiveVitro fertilisationPregnancy rateFreezingCumulusPatient'sFertilizationEmbryo transfersOvarian stimulationComplicationsEndometrial receptivityEndometriosisInfertilityViabilityRateRecipientsMiscarriageFolliclePreservationPatientsAtresiaProgesteroneFactorsImplantation ratesAdverseTissuesSheepDeclinePreserveWomenExposureRapidly
Cryopreservation23
Embryos9
  • Fluctuations in temperature and pH (acidity), along with high (20%) oxygen concentration as found in ambient air are known to adversely affect the growth of human embryos in the laboratory. (familyfertility.com)
  • Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
  • Exclusion criteria: 1) OC for a medical reason, as research, due to lack of sperm or a natural disaster, combined with embryos or for gestational carrier use, or 2) AOs/embryos from OC transported out before a LB. Primary outcome was CLBR (LB + OP). Patients (pts) were stratified by age and #AOs or metaphase II oocytes (M2s) thawed. (nyu.edu)
  • The primary outcome was number of euploid embryos. (nyu.edu)
  • Secondary outcomes were cycle start follicle stimulation hormone level (FSH), total gonadotrophin (GND) dose, number oocytes, mature oocytes (MII), fertilization rate (2PN), and number of embryos biopsied (BX). (nyu.edu)
  • If more oocytes are retrieved or embryos are formed then they can be used for donation to another woman or a couple or they can be frozen and used specifically. (acimc.org)
  • Military members who wish to preserve their fertility before deployment often will freeze sperm, oocytes, or embryos to safeguard their options for future family building. (reprotech.com)
  • Because gender-affirming hormone replacement therapy (HRT) has the possibility to negatively impact future fertility, many trans people choose to preserve and protect their fertility before beginning HRT and will work with a fertility clinic or sperm bank to freeze oocytes, embryos, or sperm for future family building. (reprotech.com)
  • The outcome of 30 such transfer cycles from 25 women where embryos were recryopreserved is detailed. (edu.au)
Fertility preservation4
Donation5
Retrieval6
Improve outcomes2
  • Antioxidants and the use of well-controlled cooling regimes could potentially improve outcomes. (iiab.me)
  • This strategy should also be explored to improve outcomes as an effectively alternative to freeze-all cycles for PCOS patients. (vitrolife.com)
ICSI3
  • Evaluating the value of day 0 of an ICSI cycle on indicating laboratory outcome. (embryokosmogenesis.gr)
  • The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles. (uzh.ch)
  • This study aimed to determine the effect of sperm DNA fragmentation (SDF) on the cumulative live birth rate (CLBR) in intracytoplasmic sperm injection (ICSI) cycles in couples with unexplained infertility. (ecerm.org)
Donor14
  • The objective of this study was to examine national trends in utilization and live birth rates with fresh versus cryopreserved donor oocytes. (biomedcentral.com)
  • A retrospective analysis of 2013 through 2015 aggregate U.S. national data reported by the Society for Assisted Reproductive Technology which included 30,160 IVF cycles with either fresh or cryopreserved donor oocytes was performed. (biomedcentral.com)
  • Over the three-year study period fresh donor oocytes produced stable live birth rates per recipient cycle start while those with cryopreserved oocytes significantly declined year-by-year. (biomedcentral.com)
  • Despite rising popularity of cryopreserved donor oocytes, prospective patients should be counselled that fresh donor oocytes still represent standard of care due to higher live birth rates. (biomedcentral.com)
  • Though ASRM recommended against routine donor oocyte banking until more robust clinical data on safety and efficacy of cryopreserved donor oocytes were generated, multiple commercial cryopreserved oocyte donor banks were nevertheless concurrently established in the U.S. Based on 2013 U.S. national data, we previously reported that live birth rates for recipients of donated cryopreserved oocytes were lower than those of fresh oocytes [ 2 ]. (biomedcentral.com)
  • Figure 1 demonstrates that live birth rates per recipient cycle start were higher with fresh donor oocytes in each of the studied years. (biomedcentral.com)
  • The use of donor oocytes has expanded the scope of assisted reproductive technology (ART) for women with poor oocyte quantity and quality. (emjreviews.com)
  • An increasing demand and a good success rate with oocyte vitrification programmes have led to the formation of oocyte banks, reducing the need for donor-recipient cycle synchronisation and allowing egg sharing. (emjreviews.com)
  • Obstetric and neonatal complications with donor oocytes are significantly increased in comparison to autologous IVF and spontaneous pregnancies. (emjreviews.com)
  • In vitro fertilisation with the use of donor oocytes (IVF-OD) has become an integral part of infertility treatment today. (emjreviews.com)
  • In the USA, the annual number of donor oocyte cycles increased from 10,801 to 18,306 between 2000 and 2010, 7 whilst the European IVF Monitoring (EIM) consortium for the European Society of Human Reproduction and Embryology (ESHRE) 4 reported 7,171 IVF-OD cycles in 2006 and 33,605 cycles in 2012. (emjreviews.com)
  • The procedure is so popular that oocyte banks 8 have been established to allow for sharing of oocytes from a donor, reduced waiting times involved in sourcing and screening donors, and circumventing donor-recipient synchronisation. (emjreviews.com)
  • The donor-recipient model has provided an insight into various aspects of ART, such as the importance of oocyte age in implantation, 10 endometrial receptivity, 11 and the contribution of male factors to IVF failure. (emjreviews.com)
  • The EIM consortium report of 2012 4 on reproductive outcome with use of donor oocytes, reported a PR of 48.4% per fresh embryo transfer, 35.9% per frozen embryo transfer (FET), and 45.1% using frozen oocytes. (emjreviews.com)
Neonatal3
  • The primary endpoint was the live birth rate, and the secondary endpoints were clinical pregnancy, miscarriage and neonatal outcomes. (biomedcentral.com)
  • This results in reduced rates of conception, increased miscarriage risk and adverse pregnancy and neonatal outcomes. (ashwinhospital.org)
  • Congenital hypothyroidism (CH) is the one of the most common preventable cause of mental retardation.The clinical features of congenital hypothyroidism are so subtle that many newborn infants remain undiagnosed at birth and delayed diagnosis leads to the most severe outcome of CH, mental retardation, emphasizing the importance of neonatal screening. (ashwinhospital.org)
Cycles13
  • To investigate whether there was a difference in pregnancy outcomes between modified natural cycle frozen-thawed embryo transfer (NC-FET) cycles and artificial cycles (AC)-FET in women who all had regular menstrual cycles. (tjoddergisi.org)
  • These findings suggest that although both FET protocols are equally effective in terms of pregnancy outcomes in women with regular menstrual cycles, NC-FET is more favorable because it requires no medication, has no adverse events, and has a significant cost reduction. (tjoddergisi.org)
  • The chemical, clinical, and ongoing pregnancy rates, miscarriage and live birth rates were compared in these two distinct FET cycles to identify predictive factors and to influence the reproductive outcome. (tjoddergisi.org)
  • While it is true that obtaining a maximum number of 15-20 oocytes for vitrification (probably in two cycles of stimulation) is relatively easy in young women, this may be more difficult in a patient with a compromised ovarian reserve, which may even be aggravated if the patient has undergone surgery", said Dr. Cobo. (ivi-fertility.com)
  • Endometriosis affects the number of retrieved oocytes but not early embryonic development and live birth: a retrospective analysis of 716 IVF cycles. (ivi-rmainnovation.com)
  • method: 77% vitrification, 4% slow cooling, 19% both), 604 thaw and 465 transfer cycles. (nyu.edu)
  • The freeze-all strategy has been developed to avoid ovarian hyperstimulation syndrome and reduced endometrial receptivity in these patients but the added vitrification and preparation in subsequent cycles increase cost and time associated with treatment significantly. (vitrolife.com)
  • Age, infertility duration, previous IVF cycles, oocyte number and stage of transferred embryo were similar in both groups as well. (vitrolife.com)
  • The scope of the presentation is to present the novelties in the Luteal phase Support strategies in modern ART, the effect of progesterone formulations in fresh and frozen embryo transfer cycles and the new treatment strategies aiming towards and individualized Luteal phase support iLPS based on the measurement of serum progesterone levels. (dexeuscampus.com)
  • The scope of the presentation is to provide an overview of the different methods of endometrial preparation for FET cycles, their advantages and disadvantages, and the evidence regarding clinical but also adverse obstetrical and perinatal outcomes, following each different protocol. (dexeuscampus.com)
  • This single-centre, large-sample retrospective trial was designed to investigate the contribution of strengthened LPS to pregnancy outcomes for groups with low progesterone levels on the FET day in artificial endometrium preparation cycles. (biomedcentral.com)
  • The results indicated that the serum progesterone level on the FET day was one of the risk factors predicting the chances of pregnancy in artificial endometrium preparation cycles, and strengthened LPS in patients with low progesterone on the FET day might help to provide a reasonable pregnancy outcome in artificial cycles, although further prospective evidence is needed to confirm this possibility. (biomedcentral.com)
  • Yildiz S, Turkgeldi E, Kalafat E, Keles I, Gokyer D, Ata B. Do live birth rate and obstetric outcomes vary between immediate and delayed embryo transfers following freeze-all cycles? (artfertilityclinics.com)
Matured in vitro1
  • After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
Survival3
  • The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth factors and steroids. (wikipedia.org)
  • This method has replaced the slow freezing protocol and shows high survival rates and favorable clinical outcomes. (molcells.org)
  • Recent studies show that age is the most powerful variable related to success after FP, which directly affects the number of oocytes vitrified, oocyte survival and cumulative live birth rate, with highest outcomes achieved by women aged ≤35y. (cambridge.org)
Clinical outcomes2
  • OBJECTIVES: To assess the epidemiological features and clinical outcomes of children and young people with inflammatory chronic diseases followed at Pediatric Rheumatology Clinics who were infected with SARS-CoV-2. (bvsalud.org)
  • However, in the frozen embryo transfers, there were no significant differences in clinical outcomes between the two groups. (ecerm.org)
Perinatal outcomes1
Fetal life1
  • The progressive loss of oocytes from fetal life through menopause is a normal process. (ldysinger.com)
Retrospective1
  • However, some controversy exists because some investigators reported better pregnancy outcomes with AC-FET, (1,2,5,13) whereas the results of some retrospective studies demonstrated the superiority of NC-FET (14,15) . (tjoddergisi.org)
Vitro fertilisation1
  • The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. (wikipedia.org)
Pregnancy rate1
Freezing9
  • However, there is still room for improvement with oocyte freezing. (focusonreproduction.eu)
  • Semen is frozen using either a controlled-rate, slow-cooling method (slow programmable freezing or SPF) or a newer flash-freezing process known as vitrification. (iiab.me)
  • Vitrification gives superior post-thaw motility and cryosurvival than slow programmable freezing . (iiab.me)
  • Egg freezing presents women with an opportunity to, in effect, stop their biological clock. (sbivf.com)
  • Women who are not ready to become pregnant now but who want to optimize their pregnancy outcomes later should consider freezing their eggs. (sbivf.com)
  • Vitrification ensures that the eggs retain their quality during the freezing and thawing process. (a4fertility.com)
  • While there isn't a strict upper age limit for egg freezing, the quality and quantity of eggs decrease with age, affecting the success of the procedure. (a4fertility.com)
  • While egg freezing offers the possibility of extending fertility options, it's important to be realistic about potential outcomes. (a4fertility.com)
  • Vitrification, the most commonly used method, involves rapid freezing, reducing the formation of ice crystals and potential damage to the eggs. (parkwayeast.com.sg)
Cumulus2
  • Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
  • Oocytes are classified depending on their condition, such as number of cumulus cells. (wikipedia.org)
Patient's1
  • By 1991, the first human pregnancy was recorded using IVM followed by IVF, and in 1994 the first birth using IVM oocytes from polycystic ovarian syndrome patients was recorded highlighting that PCOS patient's oocytes are capable of maturation. (wikipedia.org)
Fertilization5
  • In 1935, Pincus & Enzmann did the first experiment on immature rabbit oocyte, showing in vitro spontaneous maturation and fertilization. (wikipedia.org)
  • During ovulation, the primary oocyte will resume meiosis in response to signals, arresting in metaphase meiosis II, ready for fertilization. (wikipedia.org)
  • Effect of a Baseline Ovarian Cyst on the Outcome of in Vitro Fertilization-embryo Transfer. (ecerm.org)
  • Fertilization and in vitro Development of Bovine Oocytes Following Round Spermatid Injection. (ecerm.org)
  • 11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
Embryo transfers2
  • Polycystic ovary syndrome (PCOS) is known to affect implantation rates, miscarriage rates and live birth rates in fresh embryo transfers. (vitrolife.com)
  • IVF productivity rate is an index defined as the sum of all live births from either fresh or frozen embryo transfers arising from a single oocyte collection. (edu.au)
Ovarian stimulation1
  • Oocyte donors and mRNA Covid-19 vaccination: Is there any impact on ovarian stimulation parameters or in IVF outcomes for recipients? (institutobernabeu.com)
Complications3
  • Autoimmune diseases increase the complications risk and adverse pregnancy outcomes, and have also been linked in some studies to increased risk of infertility and sterility of autoimmune origin . (institutobernabeu.com)
  • It affects multiple organs such as the kidneys, heart, lungs, skin… Although currently most of these patients have good gestational outcomes, they are at increased risk of complications during pregnancy such as pre-eclampsia, preterm birth, intrauterine growth retardation (IUGR), repeated miscarriage or stillbirth. (institutobernabeu.com)
  • They also face a higher risk of pregnancy complications, such as diseases that can affect fertility like endometriosis, and miscarriages. (sbivf.com)
Endometrial receptivity1
  • ABOUBAKRELNASHAR Impact of endometriosis on the oocyte C. Racowsky et al  Women with E tend to have lower implantation rates than those without E.  Whether this is due to compromised endometrial receptivity or reduced embryo quality remains controversial. (slideshare.net)
Endometriosis4
  • How many oocytes should women with endometriosis vitrify? (ivi-fertility.com)
  • Thus, in patients under 35 years of age with endometriosis, a 95% success rate was achieved, retrieving approximately 20 oocytes, while the maximum new-born rate was close to 80% in patients over 35 years of age. (ivi-fertility.com)
  • This research aims to help fertility specialists and endometriosis patients set realistic expectations about their chances of reproductive success based on their vitrified oocytes", explained Dr. Cobo. (ivi-fertility.com)
  • Different studies have produced conflicting results when trying to explain how endometriosis affects fertility. (cnyfertility.com)
Infertility1
  • Infertility affects approximately 15% of couples of reproductive age [ 1 ]. (ecerm.org)
Viability3
  • The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
  • On the other hand, the exact thawing temperature seems to have only minor effect on sperm viability, acrosomal status, ATP content, and DNA. (iiab.me)
  • There is limited data regarding the effect of storage time on egg viability and rate of live birth. (ovinity.com)
Rate3
  • Studies support the conclusion that oocyte quality is the main factor compromising implantation rate. (slideshare.net)
  • The objective of this study by Kandari (2019) was to evaluate whether the use of embryo transfer medium enriched with hyaluronan (EmbryoGlue) improves outcomes like implantation rates, live birth rate and miscarriage rates in time-lapse selected fresh SET in PCOS women. (vitrolife.com)
  • As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)
Recipients1
  • Results achieved in recipients surpass those attained with use of autologous oocyte IVF 4-6 in good prognosis patients, resulting in an exponential rise in the procedure. (emjreviews.com)
Miscarriage1
  • There is much controversy about whether fertility may be affected in women with MS. Some studies have seen a lower ovarian reserve in women with uncontrolled MS. No increased risk of miscarriage, caesarean section or ectopic pregnancy has been seen in these patients. (institutobernabeu.com)
Follicle4
  • These then begin meiosis to form the oocyte within the primordial follicle. (wikipedia.org)
  • This follicle consists of the oocyte surrounded by flattened pregranulosa cells. (wikipedia.org)
  • Primordial follicles containing the primary oocyte, arrested at prophase of meiosis I, develop into primary follicle containing cuboidal granulosa cells. (wikipedia.org)
  • The dominant follicle contains the mature oocyte. (wikipedia.org)
Preservation1
  • Vitrifaction by ultrarapid cooling and warming is considered a standard approach in cattle oocyte preservation. (jove.com)
Patients2
  • It is just another example of our commitment to bring the latest technological improvement into our laboratory and our goal to achieve the best possible clinical outcome for our patients. (familyfertility.com)
  • 1 The images in Panel B - which shows histologic specimens of oocytes (arrows) taken from patients at birth, at the age of 25 years, and at the age of 50 years - are adapted from Erickson 8 and are reprinted with the permission of the publisher. (ldysinger.com)
Atresia4
  • Of these primordial follicles, only around 400 mature oocytes are released and could be potentially fertilised, with the rest undergoing atresia. (wikipedia.org)
  • Female infants have 6 to 7 million oocytes at 20 weeks of gestation, when progressive atresia occurs, resulting in 1 to 2 million oocytes at birth, approximately 25,000 at the age of 37 years, and 1000 at the age of 51 years, the average age of natural menopause in the United States. (ldysinger.com)
  • It has been hypothesized that in gonadal dysgenesis various deletions on the X chromosome cause such rapid atresia of oocytes 5 that few girls with this condition reach puberty with functioning ovaries. (ldysinger.com)
  • In normal women, at approximately 37.5 years of age, an accelerated atresia of the oocytes begins 1 , 2 ( Figure 1 ). (ldysinger.com)
Progesterone3
  • The results of this study suggest that once a threshold of serum progesterone before FET is achieved, progesterone levels are not predictive of the clinical outcome. (dexeuscampus.com)
  • Actually, LBR are not negatively affected when progesterone levels are found in their highest centiles after luteal phase rescue with vaginal plus subcutaneous progesterone. (dexeuscampus.com)
  • However, body mass index (BMI) and the vaginal environment affect serum levels after vaginal administration, and serum progesterone concentrations show a marked inter-individual difference even when similar doses of progesterone are administered by the vaginal route [ 4 ]. (biomedcentral.com)
Factors1
  • It is clear that the number of vitrified oocytes and the age of the patient are key factors for reproductive success. (ivi-fertility.com)
Implantation rates1
  • Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. (molcells.org)
Adverse2
  • Adverse Effect of Human Hydrosalpingeal Fluid on the Development of Mouse Embryo (II). (ecerm.org)
  • Research has shown that abnormalities in thyroid function can have an adverse effect on reproductive health - before, during and after conception. (ashwinhospital.org)
Tissues1
  • The ontogeny evaluation of the key nucleases responsible for ASO metabolism and pharmacologic activity revealed a differential nuclease expression and activity, which could affect the metabolic pathway and pharmacologic effect of ASOs in different tissues and age groups. (uantwerpen.be)
Sheep1
Decline2
  • Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
  • Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)
Preserve1
  • In this regard, a previous study, also led by Dr. Cobo, showed that young women achieved better outcomes when they had oocytes vitrified to preserve fertility prior to surgical removal of the ovarian endometrioma. (ivi-fertility.com)
Women4
  • The procedure is used to achieve pregnancy in women with premature and age-related ovarian failure, poor ovarian reserve (due to disease or advanced age), Turner's syndrome, recurrent implantation failure due to poor oocyte quality, and recurrent abortions. (emjreviews.com)
  • It affects mostly women, and the symptoms onset age is usually around 35 years old, so it affects women of childbearing age. (institutobernabeu.com)
  • Women facing medical treatments that could affect fertility, such as chemotherapy, can safeguard their ability to have biological children in the future. (a4fertility.com)
  • Nonetheless, women in the 'over 40's' can also succeed with their vitrified oocytes, although their probability of success is extremely low. (cambridge.org)
Exposure2
  • The oocytes were then dehydrated by three minutes exposure to a calibration solution. (jove.com)
  • As such, we assessed potential differences in exposure/toxicity and pharmacologic effect of a model ASO (RTR5001) in the juvenile Göttingen Minipig in an 8-week repeat-dose toxicity study (weekly subcutaneous dosing starting at postnatal day 1). (uantwerpen.be)
Rapidly2