Oocyte nuclear maturation. Medical search. Frequent questions

Previously, intra- and interspecific canine embryos have been constructed by canine SCNT into canine and bovine oocytes, respectively, but this did not result in viable offspring 9 . (nature.com)
The activated oocytes were then transferred into the oviducts or uterine horns of recipient dogs at times appropriate to the embryos' developmental stages. (nature.com)
We collected an average of 12 oocytes from each female, and a total of 1,095 reconstructed canine embryos were transferred into 123 recipients. (nature.com)
2016). Gene expression, oocyte nuclear maturation and developmental competence of bovine oocytes and embryos produced after in vivo and in vitro heat shock . (up.pt)
To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
Higher percentage of MII stage oocytes in experiment 1(65.2±2.0), embryos at morula stage in experiment 2 (30.4±1.5) and experiment 3 (22.2±2.0) were obtained. (bvsalud.org)
The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
Dr. Sessions' research focused on improving the embryo culture conditions of bovine nuclear transfer and IVF embryos as well as elucidating the key signaling pathways involved in oocyte activation and characterizing the epigenetic reprogramming profiles of key genes required for proper bovine embryonic development. (fertilitydr.com)
We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. (scite.ai)
This may indicate a problem that originated from the ovarian stimulation procedure that resulted in a lower oocyte quantity together with inadequate maturity and competence of the oocytes to develop into implanted embryos (i.e., lower oocyte quality). (biomedgrid.com)
Considering that in cattle many of the oocytes aspirated and used for the in vitro production of embryos (PIVE) have not yet acquired the competence for the development one must look for alternatives that can increase this competence and, consequently, the efficiency of the PIVE. (ufla.br)
Small RNA Profile of the Cumulus Oocyte Complex and Early Embryos in the Pig. (appliedbioinfo.com)
Deep sequencing was performed on small RNA from cumulus cells, oocytes at GV and MII stages, as well as in vitro fertilized derived embryos at 60 h post fertilization (4- to 8-cell) and on Day 6 blastocysts. (appliedbioinfo.com)
[ 3-6 ] It is widely assumed that this improvement is related to the beneficial effects of GH on oocyte quality, as suggested by the observations of a higher number of oocytes collected, higher fertilization rate, and a higher number of embryos reaching the transfer stage in GH-treated patients. (medscape.com)

However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. (ipb.ac.id)

Mature oocytes become fertilizable, and their meiosis is arrested again at a species-specific stage: metaphase of the first division (MetI, many invertebrates), metaphase of the second division (MetII, most vertebrates), or G1-phase (some echinoderms and coelenterates) until fertilization. (elifesciences.org)
Rana piptens from New Jersey were kept in spring water at 4 °C. Only mature oocytes from healthy females were used. (biologists.com)
Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)

2004. Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. (ipb.ac.id)
Because the entry of oocytes into meiosis is frequently referred to as oocyte maturation, this cytoplasmic factor was called maturation promoting factor (MPF). (wikipedia.org)
This result demonstrated that mitotic entry was controlled by a diffusible cytoplasmic factor and not by a "nuclear clock. (wikipedia.org)
Concomitant with the progression of nuclear maturation, dynamic rearrangement of intracellular organelles promotes cytoplasmic maturation. (elifesciences.org)
Nuclear sodium consists of a single fraction whose concentration is similar and whose rate constant is identical with that of the rapidly exchanging cytoplasmic fraction. (biologists.com)
We have achieved a better quantitative comparison of nuclear and cytoplasmic exchange than is possible by autoradiography. (biologists.com)

These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
Mammalian ovaries are endowed with a huge number of small oocytes (primordial oocytes) in primordial follicles. (go.jp)
Oocytes were collected from aspirated porcine tertiary follicles. (biomedcentral.com)
In addition, the mRNA abundance of both genes is not increased after in vitro hormonal stimulation of full grown follicles by maturation inducing hormones. (biomedcentral.com)
In article 1, cumulus-oocyte complex (COCs) were obtained from follicles 1-3mm, considered less competent (IC) and 6-8mm, more competent (CP). (ufla.br)

The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
While at USU he gained extensive experience performing oocyte aspiration and maturation, in vitro fertilization, in vitro embryo culture, and embryo cryopreservation in the bovine model. (fertilitydr.com)

Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)
Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)

Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)

This technology has not so far been successful in dogs because of the difficulty of maturing canine oocytes in vitro . (nature.com)
For SCNT, the chromosomes of the unfertilized canine oocytes were removed by micromanipulation, and a single donor cell was transferred into each enucleated oocyte. (nature.com)
The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
In this study, the effects of 12-hour caffeine pretreatment in a biphasic IVM protocol were assessed on maturation rates of canine oocytes. (sruc.ac.uk)

In the current study, we investigated the beneficial effects of resveratrol on in vitro porcine oocyte maturation under heat stress (HS). (sagepub.com)

Abstract The objective of the current study was to investigate the synergistic impact of - Tocopherol and - Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes . (bvsalud.org)

The major aim of my PhD project is to characterize the intracellular calcium increases in the maturing oocytes and fertilized eggs, and to study their physiological functions. (szn.it)
Bedford S J, Kurokawa M, Hinrichs K, Fissore R A(2003), Intracellular calcium oscillations and activation in horse oocytes injected with stallion sperm extracts or spermatozoa. (rfgi.fr)

At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. (ipb.ac.id)
Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)

Nuclear maturation, gene expression, cumulus cell expansion, and embryonic development and quality were analyzed. (ufla.br)

Inhibiting this miRNA during in vitro maturation (IVM) increased HAS2 levels along with several markers of oocyte quality. (appliedbioinfo.com)
Several physiological parameters, such as viability, mitochondrial activity, and reactive oxygen species (ROS) levels have been also reported as useful markers of oocyte quality in marine invertebrates. (biomedcentral.com)

In vitro nuclear maturation of compact and expanded equine oocytes treated with Roscovitine. (rfgi.fr)
GH receptor is expressed in human oocytes and cumulus cells, [ 12 , 13 ] and GH has been shown to promote in vitro nuclear maturation of denuded human oocytes. (medscape.com)

Currently, its estimation is predominantly based on morphological analysis, which is subjective and does not consistently reflect the developmental competence of the oocytes. (biomedcentral.com)

Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
Cryopreservation of mammalian oocytes has been successfully applied for long-term storage of surplus eggs in mammalian assisted reproductive technology (ART), including in humans. (molcells.org)
Mammalian oocytes represent impaired quality after undergoing a process of postovulatory aging, which can be alleviated through various effective ways such as reagent treatment. (scite.ai)

In oocytes, caffeine induces the dephosphorylation of MPF by inhibiting the transcription factor Myt1 and activity of the G2 checkpoint kinase Wee1, converting inactive MPF into its active form. (molcells.org)

2010. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. (ipb.ac.id)
Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
On observe alors que ces conceptions biologiques complexes actuelles sont de plus en plus soumises à des impératifs économiques, notamment la fiabilisation des cycles de conception et de mise sur le marché. (rfgi.fr)

The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. (ipb.ac.id)
The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. (ipb.ac.id)
In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate. (ipb.ac.id)
Supplementation of - tocopherol in maturation media having - Linolenic acid and/or in embryo culture media did not further enhance in vitro maturation of oocyte or embryo production . (bvsalud.org)
Analysis of the effectiveness of caffeine and MG132 for improving somatic cell nuclear transfer (SCNT) technology using cryopreserved eggs showed that supplementation did not improve the blastocyst formation rate of cloned mouse eggs. (molcells.org)

We collected oocytes matured in vivo at metaphase II about 72 hours after ovulation by flushing the oviducts. (nature.com)
When compared between anestrus and diestrus stages for all parameters (undetermined nuclear material, germinal vesicles, germinal vesicle break down, metaphase I, metaphase II, and degenerated) in different media, the differences were found to be significant statistically in Group IIa (22.9%) and Group IIIb (35.7%) for the germinal vesicle stage (P (tubitak.gov.tr)
Oocytes were considered mature when they reached metaphase II. (sciencegate.app)

Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. (elifesciences.org)
In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)
This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. (elifesciences.org)
Therefore, the regulatory mechanisms underlying oocyte maturation and ovulation control not only the reproduction of the respective organisms but also evolutionary processes across the animal kingdom. (elifesciences.org)
Some neuropeptides have been shown to induce oocyte maturation and ovulation (or spawning) in several species of invertebrates. (elifesciences.org)
These findings demonstrate that various neuropeptides are responsible for triggering oocyte maturation and ovulation in invertebrates, and suggest that oocyte maturation and ovulation and their underlying molecular mechanisms are regulated in both a species-specific and evolutionarily conserved fashion. (elifesciences.org)
cGMP (during follicular growth) can prevent untimely oocyte meiotic resumption up to ovulation (after LH surge, after increase in LH). (kiev.ua)

The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)
Currently, the molecular mechanisms involved in induction of oocyte meiotic resumption in the pre-ovulatory follicle which may include (involve) the elimination of meiosis inhibiting factors and/or the accumulation or activation of oocyte matu-ration signals are actively studied. (kiev.ua)
The aim of this study was to assess the effects of brief, intense exposure to tobacco smoke on granulosa cell number, oocyte growth, and follicle size during puberty in female Swiss mice. (biomedcentral.com)
[ 14 ] In addition to its direct effect on the oocytes and/or cumulus cells, GH may also influence oocyte quality indirectly, through activation of insulin-like growth factor-I synthesis or promotion of follicle-stimulating hormone-induced ovarian steroidogenesis (reviewed in [ 15 ] ). (medscape.com)

The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
The aim of this study was to determine the histone acetylation pattern in oocytes of different abilities before, during and after in vitro maturation (IVM), and to evaluate whether the use of scriptaid (HDAC inhibitor) before and after meiosis resumption development competence. (ufla.br)
Such scheme of regulation of oocyte meiotic resumption is discussed (considered): the ac-tion of gonadotrophins, FSH and LH, causes increase the pro-duction of cAMP and subsequent activation of MAPK. (kiev.ua)
Whereas different FSH and LH effects on NO production in ovary are involved in the regulation of induction of oocyte meiotic resumption in mammals. (kiev.ua)
Phosphodiesterase (PDE) inhibitors have been utilized for invitro maturation (IVM) of oocytes to manipulate the meiotic resumption and progression. (sruc.ac.uk)

Here, through oocyte-specific conditional knockout of SMC2, a core component of the condensin complex, we showed that SMC2 is essential for the production of healthy eggs and early embryo development. (authorea.com)
In the oocyte, mitochondria play a central role in cellular metabolism providing energy for successful cytoplasm and nucleus maturation, fertilization, embryo development, calcium homeostasis, and apoptosis initiation. (biomedcentral.com)

Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
however, it is not per se sufficient to assure oocyte developmental competence [ 13 ]. (biomedcentral.com)

Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)
The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
May participate in oocyte maturation via the regulation of calcium homeostasis. (enquirebio.com)
A previous screen of small RNA in the porcine ovary identified the down regulation of miR-574 during oocyte maturation, although its role during this process was not established. (appliedbioinfo.com)
Similar to the expression analysis of other species, these data demonstrate dynamic expression regulation of multiple classes of non-coding RNA during oocyte maturation and development to the blastocyst stage. (appliedbioinfo.com)
[ 13 ] The precise pathophysiologic mechanism is unknown, but it is speculated to be associated with the effects of prolactin on surfactant synthesis, whole-body water regulation, or gastrointestinal maturation. (medscape.com)

It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
Conclusions: This study compared the efficiencies of resveratrol, melatonin and their combination for protecting porcine oocytes from heat stress. (sagepub.com)
In addition, its efficiency against oocyte had an adverse effect on their developmental com- HS will be compared with melatonin, another potent anti- petence by increasing GSH content and inhibiting the pro- oxidant, and the combination of melatonin with resveratrol. (sagepub.com)
Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)

Further studies showed, however, that the activity of MPF is not restricted to the entry of oocytes into meiosis. (wikipedia.org)
Meiosis in animal oocytes is arrested at prophase of the first division (ProI). (elifesciences.org)
Choi Y H, Love L B, Varner D D, Hinrichs K. (2006), Blastocyste development in equine oocytes with low meiotic competence after suppression of meiosis with roscovitine prior to in vitro maturation. (rfgi.fr)
This gain-of-function approach resulted in a 50% decrease in HAS2 expression and nearly 20% reduction in oocyte progression through meiosis. (appliedbioinfo.com)
Oocytes are highly specialized cells that undergo the unique processes of meiosis and fertilization and execute a molecular program for development [ 1 ]. (biomedcentral.com)

2009. Effect of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. (ipb.ac.id)
2011. Effect of leptin during in vitro maturation of prepubetal calf oocytes: embryonic development and relative mRNA abudances of genes involved in apoptosis and oocyte competence. (ipb.ac.id)
Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
A crucial prerequisite for fertilization and normal embryonic development is the proper synchronization of the interactions between the cytoplasm and the nucleus of the oocytes during the meiotic maturation. (szn.it)

Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. (tubitak.gov.tr)
In this review, we summarize the results regarding the activation of primordial oocytes in neonatal and adult ovaries of different species and propose a model in which ovaries of neonatal mammals contain a mixed population of both quiescent and activated primordial oocytes, while almost all primordial oocytes are quiescent in adult females. (go.jp)
FOXO3 is considered one of the molecules responsible for the dormancy of primordial oocytes in adult ovaries. (go.jp)
Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory . (bvsalud.org)

These quiescent primordial oocytes are activated, perhaps by certain mechanisms involving the interaction between stimulatory and inhibitory factors, to enter the growth phase. (go.jp)
In conclusion, Ax might provide an alternative for ameliorating the oocyte quality following aging in vitro, through the mechanisms mediated by its antioxidant properties. (scite.ai)
Molecular mechanisms regulating the meiotic maturation and fertilization of starfish and sea urchin eggs. (szn.it)
The further research is needed to better understand the new important mechanisms that regulate diffcult aspects of oocyte meiotic maturation in mammals. (kiev.ua)
Many investigations have been designed to gain new insights into the mechanisms underlying oocyte quality based on a differential analysis applied to molecular, biochemical, transcriptomic, or proteomic data. (biomedcentral.com)

Our results show that the direct targeting of HAS2 by miR-574 negatively impacts oocyte quality during IVM and that inhibiting miR-574 derepresses HAS2 expression and subsequently improves oocyte maturation. (appliedbioinfo.com)
Results indicated that 12-hour caffeine pretreatment of canine COCs improves the MII maturation rates at 72 hours and BCB+ oocytes have higher competency invitro for nuclear maturation. (sruc.ac.uk)

CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. (elifesciences.org)
Furthermore, membrane receptor capacity of the spotted seatrout ovary has been shown to be correlated with oocyte responsiveness to 17,20β,21P for maturation, assessed by the occurrence of germinal vesicles breakdown (GVBD) [ 16 ]. (biomedcentral.com)

Oocytes of Rana pipiens were incubated in 22 Na-Ringer's solution and 22 Na ‐ 23 Na exchange in the nucleus and cytoplasm followed by low-temperature microdissection. (biologists.com)
In an earlier paper, we described a low-temperature microdissection technique and its application to the determination of sodium, potassium, and water in the nucleus and cytoplasm of the mature amphibian oocyte ( Century, Fenichel & Horowitz, 1970 ). (biologists.com)
In this study, we used the low-temperature microdissection technique to investigate the 22 Na exchange in the nucleus and cytoplasm of Rana pipiens oocytes incubated in 22 Na-Ringer's solution. (biologists.com)
First, I am investigating whether the breakdown of the nuclear envelope and the intermixing of the nucleoplasm with the cytoplasm are under the control of the Ca 2+ increase in the cytoplasm (cortex) or in the nucleus. (szn.it)

In lower vertebrates, steroid-induced oocyte maturation is considered to involve membrane-bound progestin receptors. (biomedcentral.com)
subtypes alpha, beta and gamma), was recently described in vertebrates and implicated in the progestin-initiated induction of oocyte maturation in fish. (biomedcentral.com)
This second type of steroid action has been proposed to be involved in steroid-induced oocyte maturation in lower vertebrates. (biomedcentral.com)

Importantly, oxidative stress in aged oocytes was relieved through Ax treatment, based on reduced reactive oxygen species and enhanced glutathione and antioxidant gene expression. (scite.ai)
Histone acetylation regulates gene transcription and appears to be related to the acquisition of oocyte competence. (ufla.br)
Oocytes from both groups were removed from the IVM at 0, 8 and 24h for gene expression evaluations (HAT1, KAT2A, HDAC1, HDAC2, HDAC3) and immunofluorescence. (ufla.br)
Only the HAT1 gene showed expression difference in oocytes from different treatments. (ufla.br)
We conclude that the gene expression pattern of histone acetylation related genes is related to oocyte competence. (ufla.br)
Nevertheless, the complexity of the techniques applied to assess gene expression and protein profile is a major bottleneck for the use of molecular markers in the oocyte quality assessment [ 12 ]. (biomedcentral.com)

In article 2 COCs were submitted to pre-maturation (PIVM) for 6 hours in the presence or absence of scriptaid (HDAC inhibitor). (ufla.br)

2017. Insuline-Like groeth factor-I concentration in the follicular fluid of bali cattle and its role in the oocyte nuclear maturation and fertilization rate. (ipb.ac.id)
Prolactin concentration in follicular fluid during in vitro fertilization (IVF) correlates with the oocyte maturation level and fertilization rate. (medscape.com)

Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)

Successful somatic-cell nuclear transfer (SCNT) depends on the quality, availability and maturation of the animal's unfertilized oocytes. (nature.com)
Figure 1: Dog cloned by somatic-cell nuclear transfer. (nature.com)
Through somatic cell nuclear transfer, we finally obtain transgenic cattle with increased resistance to tuberculosis. (biomedcentral.com)
After completion of his PhD, Dr. Sessions worked as a Research Assistant Professor in the Department of Animal, Dairy, and Veterinary Sciences at Utah State University where he taught laboratory based biotechnology courses for both undergraduate and graduate students in addition to being a member of the USU somatic cell nuclear transfer team where he assisted in producing clones of cattle, sheep, goats, and mules. (fertilitydr.com)
Galli, C. (2005), Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type. (rfgi.fr)

This leads to depolymerisation of the nuclear lamina & breakdown of nuclear envelope into small vesicles. (wikipedia.org)
CiVP ultimately activated a maturation-promoting factor, leading to oocyte maturation via germinal vesicle breakdown. (elifesciences.org)
Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)

Aged oocytes treated with Ax showed improved yield and quality of blastocysts as well as recovered expression of maternal genes. (scite.ai)

During oocyte growth, maturation and zygote development, chromatin structure undergoes continuous changes to regulate different nuclear activities. (authorea.com)

A critical issue for their mone secretion was caused by the abnormal expression of fertility failure is the poor quality of oocyte maturation. (sagepub.com)

Some mutations in oocytes lead to infertility in the mature organism, whereas others, over a long period of time, may eventually lead to the emergence of novel species or subspecies. (elifesciences.org)
The oocytes of 4 species were studied, and all exhibited a striking nucleocytoplasmic sodium inequality. (biologists.com)
subtypes 1 and 2) has been studied since 1996 but never studied in a fish species nor in the oocyte of any animal species. (biomedcentral.com)
Our results clearly show, for the first time in any animal species, that rainbow trout PGMRC1 mRNA is present in the oocyte and has a strong expression in ovarian tissue. (biomedcentral.com)

Moreover, inhibition in apoptosis and autophagy of aged oocyte by Ax was confirmed through decreased caspase-3, cathepsin B and autophagic activities. (scite.ai)

Anatomy12

Organisms5

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes13

Embryos18

Rate of oocytes1

Mature oocytes3

Cytoplasmic6

Follicles5

Bovine3

Increased oocyte maturation rate2

Matured in vitro2

Canine oocytes4

Porcine oocyte1

Abstract1

Intracellular2

Aceto orcein4

Cumulus cell expansion1

Markers of oocyte quality2

Vitro nuclear maturation2

Competence of the oocytes1

Mammalian3

Induces1

Complexes5

Supplementation5

Metaphase3

Ovulation7

Follicle5

Resumption5

Embryo development2

Developmental competence2

Regulation7

Melatonin5

Meiosis5

Embryonic4

Ovaries4

Mechanisms5

Improves2

Ovary2

Nucleus4

Vertebrates3

Gene6

COCs1

Fertilization rate2

Significantly increased1

Somatic5

Breakdown3

Genes1

Zygote1

Secretion1

Species4

Apoptosis1