• The oocyte maturation inhibitor (OMI) is an inhibitory factor created by follicular cells during a primary oocyte maturation. (wikipedia.org)
  • CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. (elifesciences.org)
  • 2017. Insuline-Like groeth factor-I concentration in the follicular fluid of bali cattle and its role in the oocyte nuclear maturation and fertilization rate. (ipb.ac.id)
  • observed that heat stress during the follicular recruit- the regulating mechanism of oocyte maturation. (sagepub.com)
  • These data suggest that administration of silymarin in IVF patients concomitantly with gonadotropin results in reduction of granolusa cell apoptosis but does not have any effect in promotion of follicular development, oocyte retrieval or endometrial thickness. (who.int)
  • Prolactin concentration in follicular fluid during in vitro fertilization (IVF) correlates with the oocyte maturation level and fertilization rate. (medscape.com)
  • In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
  • Four immature, germinal vesicle (GV) oocytes were found and removed from tissue, denuded mechanically by a pipette, and matured in vitro in a maturation medium with added FSH and hCG as well as in co-culture with cumulus cells, which were retrieved by their denudation. (infertilityschool.ru)
  • Three oocytes matured in vitro to the metaphase II (MII) stage and were vitrified for in vitro fertilization along with ovarian tissue cryopreservation. (infertilityschool.ru)
  • Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
  • It is believed to be the reason why the oocyte remains for so long in the immature dictyate state of meiosis. (wikipedia.org)
  • Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
  • After oocyte collection, immature oocytes were cultured in the IVM medium. (springer.com)
  • Here, we show that injection of CL100 (also called MAPK phosphatase-1) into immature oocytes inhibited progesterone-induced oocyte maturation as well as MAPK activation and that injection of mRNA encoding a constitutively active MAPKK induced activation of histone H1 kinase and germinal vesicle breakdown in the absence of progesterone. (elsevierpure.com)
  • The increase in Mos protein and the activation of MAPK by injecting cyclin A protein into immature oocytes were both blocked also by cycloheximide treatment. (elsevierpure.com)
  • Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. (infertilityschool.ru)
  • Therefore, a small biopsy of ovarian cortex tissue was performed using laparoscopy to retrieve immature oocytes. (infertilityschool.ru)
  • Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. (elifesciences.org)
  • In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)
  • This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. (elifesciences.org)
  • Therefore, the regulatory mechanisms underlying oocyte maturation and ovulation control not only the reproduction of the respective organisms but also evolutionary processes across the animal kingdom. (elifesciences.org)
  • Some neuropeptides have been shown to induce oocyte maturation and ovulation (or spawning) in several species of invertebrates. (elifesciences.org)
  • These findings demonstrate that various neuropeptides are responsible for triggering oocyte maturation and ovulation in invertebrates, and suggest that oocyte maturation and ovulation and their underlying molecular mechanisms are regulated in both a species-specific and evolutionarily conserved fashion. (elifesciences.org)
  • Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). (wur.nl)
  • Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. (wur.nl)
  • After puberty, during each menstrual cycle, one or several oocytes resume meiosis and undergo their first meiotic division during ovulation. (wikibooks.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • Concomitant with the progression of nuclear maturation, dynamic rearrangement of intracellular organelles promotes cytoplasmic maturation. (elifesciences.org)
  • MIH activates the cytoplasmic maturation promoting factor (MPF), which is made up of two subunits: cyclin B (a regulatory subunit) and cdc2 (a catalytic subunit). (biomedcentral.com)
  • The signal of 1-MA is transduced into cytoplasmic formation of maturation-promoting factor (MPF) that eventually induces a germinal vesicle breakdown (GVBD). (elsevierpure.com)
  • These results indicate that in starfish oocyte membranes, 1-MA receptors are functionally coupled with the 39-kDa PTX-substrate G protein that transduces the signal into the formation of a cytoplasmic factor (MPF) and eventually into the reinitiation of meiosis. (elsevierpure.com)
  • 2004. Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. (ipb.ac.id)
  • The mature oocytes can be vitrified and stored for in vitro fertilization, which, along with ovarian tissue cryopreservation, is mostly practiced in young cancer patients to preserve their fertility. (infertilityschool.ru)
  • Conclusions Our data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8 , underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF). (bmj.com)
  • Ovarian development in fish is broadly divided into two major phases: growth and maturation. (biomedcentral.com)
  • In experiment III, worms collected in spring were exposed to different temperature regimes to examine the effect of raising temperature on the synchronization of oocyte growth and maturation. (frontiersin.org)
  • The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. (springer.com)
  • Phosphodiesterase (PDE) inhibitors have been utilized for invitro maturation (IVM) of oocytes to manipulate the meiotic resumption and progression. (sruc.ac.uk)
  • In this study we evaluated the expression of CTSL during oocyte meiotic maturation and the effect of CTSL inhibition using specific inhibitor "SCP110" on the subsequent maturation process. (advetresearch.com)
  • These mutations cause a range of folding defects in vitro, different degrees of microtubule disruption upon expression in cultured cells and interfere to varying extents in the proper assembly of the meiotic spindle in mouse oocytes. (bmj.com)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • However, as the oocyte grows, these proteins are synthesized, and meiotic arrest becomes dependent on cyclic AMP . (wikipedia.org)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • In the oocyte, cyclic GMP prevents the breakdown of cyclic AMP by the phosphodiesterase PDE3, and thus maintains meiotic arrest. (wikipedia.org)
  • Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • Results indicated that 12-hour caffeine pretreatment of canine COCs improves the MII maturation rates at 72 hours and BCB+ oocytes have higher competency invitro for nuclear maturation. (sruc.ac.uk)
  • The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. (ipb.ac.id)
  • At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. (ipb.ac.id)
  • Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
  • GH receptor is expressed in human oocytes and cumulus cells, [ 12 , 13 ] and GH has been shown to promote in vitro nuclear maturation of denuded human oocytes. (medscape.com)
  • During oocyte growth, follicle stimulating hormone (FSH) stimulates production of estradiol-17β from the ovary. (biomedcentral.com)
  • [ 14 ] In addition to its direct effect on the oocytes and/or cumulus cells, GH may also influence oocyte quality indirectly, through activation of insulin-like growth factor-I synthesis or promotion of follicle-stimulating hormone-induced ovarian steroidogenesis (reviewed in [ 15 ] ). (medscape.com)
  • Mitogen-activated protein kinase (MAPK) and MAPK kinase (MAPKK) are activated during Xenopus oocyte maturation concomitant with the activation of maturation promoting factor (MPF). (elsevierpure.com)
  • Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). (springer.com)
  • This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
  • First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
  • Caffeine, a nonselective competitive PDE inhibitor, due to its structural similarity to adenosine molecule maintains the cAMP level by occupying PDE enzymes such as PDE-3A inside the oocyte and PDE-4 and PDE-5 in the cumulus cells. (sruc.ac.uk)
  • Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation. (bvsalud.org)
  • Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single- cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. (bvsalud.org)
  • T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. (bvsalud.org)
  • The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
  • In this study, the effects of 12-hour caffeine pretreatment in a biphasic IVM protocol were assessed on maturation rates of canine oocytes. (sruc.ac.uk)
  • [2] It is developed from the primary oocyte by maturation. (wikipedia.org)
  • To determine if the effect of TGF-beta1 on oocyte maturation involves transcription and/or translation, ovarian follicles were pre-treated with actinomycin D, a blocker of transcription, and cyclohexamide, an inhibitor of translation, and incubated with hCG or DHP, either alone or in combination with TGF-beta1 and oocyte maturation scored. (biomedcentral.com)
  • Normal maturation and growth of fullestrogenreceptoractivation.Silybin andfromthefirstdayofovarianstimula- oocytesdependsonadequatesupport B,oneofthecomponentsofsilymarin, tion,onegroupofpatientsreceivedgo- from ovarian granolusa cel s of the has(probable)weakestrogenreceptor nadotropinplussilymarin(70mg×3/ follicles [ 1 ]. (who.int)
  • 7) the developmental competence of porcine oocytes was significantly affected by an interaction among the age of the donor animal, ovarian follicle size, and exogenous gonadotropic stimulation. (uconn.edu)
  • Recent research showed that cathepsins family play an important role in affecting the oocytes developmental competence in many species. (advetresearch.com)
  • Cathepsin B inhibition improved bovine developmental competence of the oocytes, especially in the low-quality oocytes. (advetresearch.com)
  • The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • [ 3-6 ] It is widely assumed that this improvement is related to the beneficial effects of GH on oocyte quality, as suggested by the observations of a higher number of oocytes collected, higher fertilization rate, and a higher number of embryos reaching the transfer stage in GH-treated patients. (medscape.com)
  • Moreover, in spite of the early success of equine oocyte in vitro maturation [ 2 ], no relevant research on the issue has been reported in the horse for some time. (ivis.org)
  • Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. (tubitak.gov.tr)
  • The ovaries of a newborn baby girl contain about one million oocytes. (wikibooks.org)
  • When a young woman reaches puberty around age 10 to 13, a promary oocyte is discharged from one of the ovaries every 28 days. (wikibooks.org)
  • This protein is also involved in development of the ovaries, from before birth through adulthood, and is important for development and maintenance of egg cells (oocytes) in the ovaries. (medlineplus.gov)
  • Conclusions: This study compared the efficiencies of resveratrol, melatonin and their combination for protecting porcine oocytes from heat stress. (sagepub.com)
  • This results in the production of a secondary oocyte and one polar body. (wikibooks.org)
  • We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. (springer.com)
  • The devastating impact on fertility in such disorder can be successfully avoided by in vitro maturation of oocytes from excised ovarian tissue. (infertilityschool.ru)
  • Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)
  • The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
  • It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
  • To determine the effect of TGF-beta1 on mRNA levels of several key effectors of oocyte maturation, three sets of experiments were performed. (biomedcentral.com)
  • However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. (ipb.ac.id)
  • In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate. (ipb.ac.id)
  • 2009. Effect of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. (ipb.ac.id)
  • ICSI produced the first pregnancy derived from an in vitro matured oocyte [ 5 ], and it was successfully carried to term. (ivis.org)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
  • Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)
  • In addition, its efficiency against oocyte had an adverse effect on their developmental com- HS will be compared with melatonin, another potent anti- petence by increasing GSH content and inhibiting the pro- oxidant, and the combination of melatonin with resveratrol. (sagepub.com)
  • 2010. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. (ipb.ac.id)
  • A critical issue for their mone secretion was caused by the abnormal expression of fertility failure is the poor quality of oocyte maturation. (sagepub.com)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. (ipb.ac.id)
  • The thesis investigated the various factors influencing porcine in vitro maturation of an oocyte (IVM), fertilization (IVF), and early embryo culture (IVC). (uconn.edu)
  • Background TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. (bmj.com)
  • For example, oocytes harbouring any of three missense mutations (I210V, T238M and N348S) could extrude the first polar body. (bmj.com)
  • In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
  • In Caenorhabditis elegans, germline immortality involves proteostasis renewal at the beginning of each new generation, when oocyte maturation signals from sperm trigger the clearance of carbonylated proteins and protein aggregates. (elsevierpure.com)
  • Oocyte maturation signals are known to trigger protein-aggregate removal via lysosome acidification. (elsevierpure.com)
  • Injection of recombinant STE11 protein (a yeast MAPKK kinase) also induced initiation of oocyte maturation. (elsevierpure.com)
  • Indeed, plasma membrane preparations of A. pectinifera oocytes contained a G protein consisting of 39-kDa α, 37-kDa β, and 8-kDa γ subunits. (elsevierpure.com)
  • The adenylyl cyclase is kept active by a constitutively active G-protein-coupled receptor known as GPR3 and a G-protein, Gs, also present in the oocyte membrane. (wikipedia.org)
  • Some mutations in oocytes lead to infertility in the mature organism, whereas others, over a long period of time, may eventually lead to the emergence of novel species or subspecies. (elifesciences.org)
  • We previously found that mutations in TUBB8 caused oocyte maturation arrest. (bmj.com)
  • We investigated the effects of identified mutations in vitro, in cultured cells and in mouse oocytes. (bmj.com)
  • Surprisingly, oocytes from patients harbouring homozygous TUBB8 mutations that in either case preclude the expression of a functional TUBB8 polypeptide nonetheless contained identifiable spindles. (bmj.com)
  • The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation , such as mammalian oocytes . (bvsalud.org)
  • Galvão A, Segers I, Smitz J, Tournaye H, De Vos M. In vitro maturation (IVM) of oocytes in patients with resistant ovary syndrome and in patients with repeated deficient oocyte maturation. (springer.com)
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • Design and Study Population A total of 105 infertile women were enrolled in the randomized controlled trial: 70 women were with a history of RIF with donated oocytes, and 35 infertile women underwent the first oocyte donation attempt. (medscape.com)
  • PTX-inhibition of GVBD was rescued by the injection of MPF into PTX-preinjected oocytes. (elsevierpure.com)
  • Moreover, CTSL inhibition reduced the maturation rate compared to the control group as we found a significant decrease in PBE percent. (advetresearch.com)
  • Finally, we evaluated matured oocytes quality after CTSL inhibition, importantly, aneuploidy incidence was increased significantly after CTSL inhibition. (advetresearch.com)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
  • The number and quality of oocytes retrieved were evaluated and apoptosis of granolusa cells was studied. (who.int)
  • Therefore, apoptosis of (ER)-mediatedactivity.Silybin-Aand day)andtheothergroupgonadotropin granolusacellsseemstohaveanegative otherflavonolignansareinactive,and plusplacebo.Transvaginalultrasound effectonfolliculardevelopment,oocyte taxifolin,whichisaminorconstituent examinationwasperformedtoevaluate qualityandpregnancyrates[ 2 ]. (who.int)
  • This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)
  • The result showed worms had 1.5 times faster oocyte growth rates when exposed to accelerated rate of change in photoperiod (2.5 times faster). (frontiersin.org)
  • The data indicated that leptin could improve maturation rate in lower concentration. (ipb.ac.id)
  • We reported previously that an anti-MAPKK neutralizing antibody inhibited progesterone- or Mos- induced initiation of oocyte maturation. (elsevierpure.com)
  • These results are consistent with an idea that there may exist a positive feedback loop consisting of Mos, the MAPKK/MAPK cascade, and MPF, which may be important for the initiation of oocyte maturation induced by progesterone. (elsevierpure.com)
  • Treatment with actinomycin D, a blocker of transcription, reduced the inhibitory effect of TGF-beta1 on DHP-induced oocyte maturation, indicating that the inhibitory action of TGF-beta1 is in part due to regulation of gene transcription. (biomedcentral.com)
  • HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
  • [ 13 ] The precise pathophysiologic mechanism is unknown, but it is speculated to be associated with the effects of prolactin on surfactant synthesis, whole-body water regulation, or gastrointestinal maturation. (medscape.com)
  • The result showed females collected during summer showed the highest oocyte growth when exposed to constant temperatures combined with decreasing photoperiod and decreasing temperature combined with constant photoperiod. (frontiersin.org)
  • Today, the most practical use of OPU or transabdominal follicle aspiration is to recover in vivo matured oocytes [ 9 ] for oocyte transfer. (ivis.org)
  • These data support the idea that the MAPKK/MAPK cascade plays an important role in oocyte maturation. (elsevierpure.com)
  • Oocyte maturation in teleosts is triggered by the release of leutinizing hormone (LH) from the pituitary. (biomedcentral.com)
  • The results showed worms collected in spring showed increased temperatures will increase oocyte maturation synchronicity. (frontiersin.org)