Oocyte maturation inhibitor. Medical search. Frequent questions

Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1). (nih.gov)

Concomitant with the progression of nuclear maturation, dynamic rearrangement of intracellular organelles promotes cytoplasmic maturation. (elifesciences.org)
However, as compared with oocytes without aging, it was found that aging significantly inhibited nuclear maturation, impaired mitochondria function, and damaged the spindle and DNA. (monossabios.com)
Results indicated that 12-hour caffeine pretreatment of canine COCs improves the MII maturation rates at 72 hours and BCB+ oocytes have higher competency invitro for nuclear maturation. (sruc.ac.uk)

Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. (elifesciences.org)
In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)
This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. (elifesciences.org)
Therefore, the regulatory mechanisms underlying oocyte maturation and ovulation control not only the reproduction of the respective organisms but also evolutionary processes across the animal kingdom. (elifesciences.org)
Some neuropeptides have been shown to induce oocyte maturation and ovulation (or spawning) in several species of invertebrates. (elifesciences.org)
These findings demonstrate that various neuropeptides are responsible for triggering oocyte maturation and ovulation in invertebrates, and suggest that oocyte maturation and ovulation and their underlying molecular mechanisms are regulated in both a species-specific and evolutionarily conserved fashion. (elifesciences.org)
cGMP (during follicular growth) can prevent untimely oocyte meiotic resumption up to ovulation (after LH surge, after increase in LH). (kiev.ua)
Considering that PF constantly surrounds the ovaries and has direct contact with the oocyte at ovulation, we wonder if PF from women with endometriosis may affect antioxidant enzyme gene expression. (springer.com)
These findings raise the possibility of a deleterious influence of PF from women with EI/II on the oocyte, not only after ovulation but also during the maturation process, which could contribute to worsening oocyte quality, being one of the mechanisms related to infertility in patients with endometriosis. (springer.com)
The function of miRNA in the ovary was indicated by the actual fact that knockout of Dicer the ribonuclease III that's responsible for the formation of older useful miRNA in the ovary led to the dysfunction of folliculogenesis oocyte maturation ovulation and infertility (2-6). (bio-cavagnou.info)

It is believed to be the reason why the oocyte remains for so long in the immature dictyate state of meiosis. (wikipedia.org)
Meiosis in animal oocytes is arrested at prophase of the first division (ProI). (elifesciences.org)
Mature oocytes become fertilizable, and their meiosis is arrested again at a species-specific stage: metaphase of the first division (MetI, many invertebrates), metaphase of the second division (MetII, most vertebrates), or G1-phase (some echinoderms and coelenterates) until fertilization. (elifesciences.org)
Currently, the molecular mechanisms involved in induction of oocyte meiotic resumption in the pre-ovulatory follicle which may include (involve) the elimination of meiosis inhibiting factors and/or the accumulation or activation of oocyte matu-ration signals are actively studied. (kiev.ua)
However, its functional roles in oocyte meiosis have not been fully determined. (biomedcentral.com)
Although increasing studies have demonstrated the essential functions of CK2 in the various biological processes, its exact role in mammalian oocyte meiosis remains largely unknown. (biomedcentral.com)
We found, CTSL is expressed in all stages of Meiosis I. In addition, inhibition of CTSL resulted in delaying the maturation time by elongation the time required for extrusion of the polar body (PBE). (advetresearch.com)
In summary, CTSL is required for normal meiosis completion in oocytes and production of healthy euploid egg. (advetresearch.com)
Some of thesignaling pathways identified are critical for development, like steroid hormone biosynthesis (ko00140), ovarian steroidogenesis (ko04913), Gnrh signaling pathway (ko04912), cortisol synthesis and secretion (ko04927), estrogen signaling pathway (ko04915), steroid biosynthesis (ko00100), oocyte meiosis (ko04114), and progesterone-mediated oocyte maturation (ko04914). (trpv1inhibitor.com)
PDE3 inhibitors suppress meiosis in oocytes by decreasing the level of cAMP and blocking the extrusion of the first polar body. (cancer-research-network.com)

Microarray applied sciences are helpful to mine the transcriptome of FCs expressed in follicles related to competent oocytes and might be used to enhance embryo choice with the target of profitable single embryo switch. (serpins.com)
Adona PR, Lima Verde Leal C (2004) Meiotic inhibition with different cyclin-dependent kinase inhibitors in bovine oocytes and its effects on maturation and embryo development. (springer.com)
Studies conducted at different time points during the progression from oocyte to embryo suggest that RSK1 and Plk1 share a close relationship. (biospraysehatalami.com)
Most egg yolk precursors are synthesized by the liver, secreted into the blood and transferred into oocytes, to provide nutrients and bioactive molecules for the avian embryo. (biomedcentral.com)

Trying to understand the reason of the lower maturation rates, we stained the chromosomes and spindles at metaphase I stage, we found a significant increase in chromosomal misalignment at metaphase plate. (advetresearch.com)
Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. (techscience.com)
Two analyses were performed: the first involved CC of immature metaphase I (MI) and mature metaphase II (MII) oocytes where 359 genes were differentially expressed, and the second involved the two GnRH analogues where no differentially expressed genes were observed at the entire transcriptome level. (elsevierpure.com)
Degradation of Mos inactivates p90RSK when mature metaphase II-arrested oocytes are fertilized (25-27) and Myt1 forms a complex with Plk1. (biospraysehatalami.com)

In oocytes, caffeine induces the dephosphorylation of MPF by inhibiting the transcription factor Myt1 and activity of the G2 checkpoint kinase Wee1, converting inactive MPF into its active form. (molcells.org)

To characterize the effect of artificially increasing MIR21 on oocyte competence without inhibiting GVBD, a MIR21 mimic, scrambled microRNA negative control, or nuclease free water was micro-injected into denuded oocytes at 21 h of IVM. (biomedcentral.com)
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
thus oocyte developmental competence could also be decided by markers expressed within the surrounding FCs. (serpins.com)
Recent research showed that cathepsins family play an important role in affecting the oocytes developmental competence in many species. (advetresearch.com)
Cathepsin B inhibition improved bovine developmental competence of the oocytes, especially in the low-quality oocytes. (advetresearch.com)

CX-4945, as a first oral bioavailable CK2 small molecule inhibitor, exerts anti-proliferative activity in human cancer cells by inhibiting cell cycle and PI3K/Akt signaling pathway [ 17 ]. (biomedcentral.com)
Weighed against its homologs Plk1 (and its own connected signaling pathway) offers attracted much interest because overexpression of Plk1 can be firmly correlated with carcinogenesis (10 11 Furthermore inhibition of Plk1 using RNA disturbance (RNAi) or particular little molecule inhibitors causes development arrest or apoptosis in tumor cells (12-14). (biospraysehatalami.com)

Ovarian development in fish is broadly divided into two major phases: growth and maturation. (biomedcentral.com)
Further, melatonin and its receptors play a significant role in antioxidant defense mechanism of ovary for follicular growth and maturation. (jrhm.org)

During oocyte growth, follicle stimulating hormone (FSH) stimulates production of estradiol-17β from the ovary. (biomedcentral.com)
A further analysis of 359 differentially genes was performed, focusing on anti-Müllerian hormone receptor 2 (AMHR2), follicle stimulating hormone receptor (FSHR), vascular endothelial growth factor C (VEGFC) and serine protease inhibitor E2 (SERPINE2). (elsevierpure.com)

We report that CK2 is essential for porcine oocyte meiotic maturation by regulating spindle assembly checkpoint (SAC). (biomedcentral.com)
Our findings demonstrate that CK2 promotes the porcine oocyte maturation by ensuring normal spindle assembly and DNA damage repair. (biomedcentral.com)
In the current study, we explored the functional roles of CK2 on the porcine oocyte meiotic maturation. (biomedcentral.com)

MIH activates the cytoplasmic maturation promoting factor (MPF), which is made up of two subunits: cyclin B (a regulatory subunit) and cdc2 (a catalytic subunit). (biomedcentral.com)

The oocyte must maintain arrest at the diplotene stage until meiotic resumption occurs. (biomedcentral.com)
Such scheme of regulation of oocyte meiotic resumption is discussed (considered): the ac-tion of gonadotrophins, FSH and LH, causes increase the pro-duction of cAMP and subsequent activation of MAPK. (kiev.ua)
Whereas different FSH and LH effects on NO production in ovary are involved in the regulation of induction of oocyte meiotic resumption in mammals. (kiev.ua)
Phosphodiesterase (PDE) inhibitors have been utilized for invitro maturation (IVM) of oocytes to manipulate the meiotic resumption and progression. (sruc.ac.uk)

inhibitor clade E member 2 (P = 0.0499), Cytochrome P450 aromatase (P = 0.0359) and Cell division cycle 42 (P = 0.0396). (serpins.com)
The available medical treatments for PCOS-related infertility-including antiestrogens, aromatase inhibitors, insulin-sensitizing drugs, and gonadotropins-are reviewed, and other potential therapeutic approaches, such as acupuncture and laparoscopic ovarian drilling, are assessed. (nshealth.ca)
Aromatase Inhibitors - 11. (nshealth.ca)

Immature bovine oocytes underwent in vitro maturation in the absence of PF and in the presence of three concentrations (1, 5 and 10%) of PF from fertile and from infertile women with EI/II. (springer.com)

Thus, the present study aims to evaluate the PF impact from infertile women with minimal and mild endometriosis and from fertile control women without endometriosis on SOD1 , CAT , GSR gene's expression in experimental bovine oocytes matured in vitro. (springer.com)
Ali AA, Bilodeau JF, Sirard MA (2003) Antioxidant enzyme activity and oxidative stress in bovine oocyte in vitro maturation. (springer.com)
Bettegowda A, Patel OV, Ireland JJ, Smith GW (2006) Quantitative analysis of messenger RNA abundance for ribosomal protein L-15, cyclophilin-A, phosphoglycerokinase, beta-glucuronidase, glyceraldehyde 3-phosphate dehydrogenase, beta-actin, and histone H2A during bovine oocyte maturation and early embryogenesis. (springer.com)
In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. (techscience.com)
In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. (techscience.com)

Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)

2006). Our focus is usually on the function of MSI1 in tumorigenesis and on the development of small molecule inhibitors of MSI1 as a possible novel therapeutic approach. (thetechnoant.info)
While previous studies have recognized small molecule inhibitors of MSI family proteins, none of these studies assessed anti\malignancy activities (Clingman et?al. (thetechnoant.info)

Some mutations in oocytes lead to infertility in the mature organism, whereas others, over a long period of time, may eventually lead to the emergence of novel species or subspecies. (elifesciences.org)
The book opens by discussing anovulation, oocyte quality, and the endometrium in women with PCOS, infertility and subfertility cofactors, and the impact of PCOS phenotypes on fertility. (nshealth.ca)

Prolactin concentration in follicular fluid during in vitro fertilization (IVF) correlates with the oocyte maturation level and fertilization rate. (medscape.com)
Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
The use of controlled ovarian stimulation in infertile PCOS patients undergoing intrauterine insemination or in vitro fertilization is examined in detail, as are the benefits of in vitro maturation of oocytes. (nshealth.ca)
Usage of melatonin during in vitro fertilization (IVF) procedures has been found to improve oocyte quality, survival, and fecundity. (jrhm.org)
Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)

To determine if the effect of TGF-beta1 on oocyte maturation involves transcription and/or translation, ovarian follicles were pre-treated with actinomycin D, a blocker of transcription, and cyclohexamide, an inhibitor of translation, and incubated with hCG or DHP, either alone or in combination with TGF-beta1 and oocyte maturation scored. (biomedcentral.com)
Oocytes were collected from aspirated porcine tertiary follicles. (biomedcentral.com)

Although chromosome missegregation during oocyte maturation (OM) is a significant contributor to human morbidity and mortality, very little is known about the causes and mechanisms of aneuploidy. (biomedcentral.com)
In addition, we found that spindle/chromosome structure was disrupted in CK2-inhibited oocytes due to the weakened microtubule stability, which is a major cause resulting in the activation of SAC. (biomedcentral.com)

Treatment with actinomycin D, a blocker of transcription, reduced the inhibitory effect of TGF-beta1 on DHP-induced oocyte maturation, indicating that the inhibitory action of TGF-beta1 is in part due to regulation of gene transcription. (biomedcentral.com)
[ 15 ] The precise pathophysiologic mechanism is unknown, but it is speculated to be associated with the effects of prolactin on surfactant synthesis, whole-body water regulation, or gastrointestinal maturation. (medscape.com)
Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)
The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)

CK2β directly binds and inhibits Mos to prevent premature MAPK activation and oocyte maturation [ 22 ]. (biomedcentral.com)

Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. (techscience.com)
CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor. (techscience.com)
The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula transition and segmentation. (biomedcentral.com)
Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction and focal adhesion, suggesting high complexity in terms of structure and cellular interactions in embryos prior to midblastula transition (MBT). (biomedcentral.com)

Recently, we showed that TGF-beta1 inhibits gonadotropin- and 17alpha, 20beta-dihydroxyprogesterone (DHP)-induced maturation in zebrafish. (biomedcentral.com)

Finally, we evaluated matured oocytes quality after CTSL inhibition, importantly, aneuploidy incidence was increased significantly after CTSL inhibition. (advetresearch.com)

CiErk1/2 also induced expression of matrix metalloproteinase (CiMMP2/9/13) in the oocyte, resulting in collagen degradation in the outer follicular cell layer and liberation of fertile oocytes from the ovary. (elifesciences.org)

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. (cusabio.com)

Oocyte arrest at the diplotene stage is maintained in part through the activity of phosphodiesterase enzymes that catalyze the hydrolysis of cyclic adenosine monophosphate (cAMP) into AMP [ 1 , 2 ]. (biomedcentral.com)
During oocyte maturation RSK both phosphorylates and down-regulates Myt1 causing prophase I arrest (24). (biospraysehatalami.com)

Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)
Samples were spun down and washed five times in PBS sup kinase inhibitor Thiazovivin plemented with 5% FBS and then plated onto 10 cm collagen coated plates in DMEM/F12 media supplemented with 2% FBS and penicillin/streptomycin. (mek-inhibitors.com)

Inhibition of CK2 activity by its selective inhibitor CX-4945 impaired the first polar body extrusion and arrested oocytes at M I stage, accompanied by the presence of BubR1 at kinetochores, indicative of activated SAC. (biomedcentral.com)
We also employed the CK2-specific inhibitor CX-4945 to validate the effect of CK2 activity on the polar body extrusion, spindle assembly, microtubule stability and DNA damage level in porcine oocytes. (biomedcentral.com)

Caffeine, a nonselective competitive PDE inhibitor, due to its structural similarity to adenosine molecule maintains the cAMP level by occupying PDE enzymes such as PDE-3A inside the oocyte and PDE-4 and PDE-5 in the cumulus cells. (sruc.ac.uk)
The aim of our study was to improve the understanding of gene expression profile of cumulus cells (CC) in terms of ovarian stimulation protocol and oocyte maturity. (elsevierpure.com)

CiVP ultimately activated a maturation-promoting factor, leading to oocyte maturation via germinal vesicle breakdown. (elifesciences.org)
Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)

Controlled Ovarian Stimulation and In Vitro Oocyte Maturation -- 18. (nshealth.ca)

Actin and myosin inhibitors often blocked anaphase movements in insect spermatocytes in previous experiments. (biomedcentral.com)
Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. (techscience.com)

We applied Affymetrix gene expression profiling in CC of oocytes at different maturation stages using either GnRH agonists or GnRH antagonists. (elsevierpure.com)

Studies have demonstrated oxidative stress in peritoneal fluid (PF) from women with endometriosis and the importance of enzymatic antioxidant machinery to avoid oocyte oxidative damage. (springer.com)

Endothelial protease nexin-1 is a novel regulator of A disintegrin and metalloproteinase 17 maturation and endothelial protein C receptor shedding via furin inhibition. (cusabio.com)

The oocyte maturation inhibitor (OMI) is an inhibitory factor created by follicular cells during a primary oocyte maturation. (wikipedia.org)

Immunostaining and immunoblotting analysis showed that CK2 was constantly expressed and located on the chromosomes during the entire oocyte meiotic maturation. (biomedcentral.com)

Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)

Our results indicate MIR21 is active and important during meiotic maturation of the oocyte. (biomedcentral.com)

Here, we evaluated MPF activity in vitrified mouse eggs following treatment with caffeine, a known stimulator of MPF activity, and/or the proteasome inhibitor MG132. (molcells.org)
Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. (molcells.org)
In this study, the effects of 12-hour caffeine pretreatment in a biphasic IVM protocol were assessed on maturation rates of canine oocytes. (sruc.ac.uk)

The PAI-1 inhibitor not only accelerated the expansion of the donor HSCs during the early-stage of regeneration, but also supported long-term hematopoiesis. (cusabio.com)

Anatomy27

Organisms20

Diseases4

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes53

Disciplines and Occupations1

Information Science4

Health Care1

Mouse oocytes1

Nuclear maturation3

Ovulation10

Meiosis10

Embryo4

Metaphase4

Induces1

Competence5

Signaling pathway2

Growth and maturation2

Follicle stimula2

Porcine oocyte3

Cytoplasmic maturation1

Meiotic resumption4

Aromatase3

Immature1

Bovine5

Mammalian oocyte1

Small molecule inhibitors2

Infertility2

Fertilization5

Follicles2

Chromosome2

Regulation4

MAPK1

Embryos5

Inhibits1

Aneuploidy1

Ovary1

Serine1

Arrest2

Kinase2

Extrusion2

Cumulus cells2

Germinal vesicle2

Ovarian Stimulation1

Actin2

GnRH1

Oxidative1

Protein1

Inhibitory1

Chromosomes1

Significantly1

Results indicate1

Caffeine3

Donor1