• Ascidians are the closest living relatives of vertebrates, and their study is important for understanding the evolutionary processes of oocyte maturation and ovulation. (elifesciences.org)
  • In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)
  • This is the first demonstration of essential pathways regulating oocyte maturation and ovulation in ascidians and will facilitate investigations of the evolutionary process of peptidergic regulation of oocyte maturation and ovulation throughout the phylum Chordata. (elifesciences.org)
  • Therefore, the regulatory mechanisms underlying oocyte maturation and ovulation control not only the reproduction of the respective organisms but also evolutionary processes across the animal kingdom. (elifesciences.org)
  • Some neuropeptides have been shown to induce oocyte maturation and ovulation (or spawning) in several species of invertebrates. (elifesciences.org)
  • These findings demonstrate that various neuropeptides are responsible for triggering oocyte maturation and ovulation in invertebrates, and suggest that oocyte maturation and ovulation and their underlying molecular mechanisms are regulated in both a species-specific and evolutionarily conserved fashion. (elifesciences.org)
  • Ovulation in European eel is induced by injection of 17α,20β-dihydroxy-4-pregnen-3-one (DHP) as the maturation-inducing hormone (MIH). (wur.nl)
  • Expression of genes involved in oocyte maturation and ovulation was similar in vitro for both DHP and P treatments. (wur.nl)
  • Our previous study demonstrated that both kissl and kiss2 mRNA levels increased during final oocyte maturation (FOM) and ovulation in the spawning cycle, and suggested their possible involvement in the regulation of these two processes. (elsevierpure.com)
  • After puberty, during each menstrual cycle, one or several oocytes resume meiosis and undergo their first meiotic division during ovulation. (wikibooks.org)
  • As follicles grow, they acquire receptors for luteinizing hormone, a pituitary hormone that reinitiates meiosis in the oocyte and causes ovulation of a fertilizable egg. (wikipedia.org)
  • These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)
  • The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
  • 2009. Effect of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse. (ipb.ac.id)
  • 2011. Effect of leptin during in vitro maturation of prepubetal calf oocytes: embryonic development and relative mRNA abudances of genes involved in apoptosis and oocyte competence. (ipb.ac.id)
  • Oogonia enter meiosis during embryonic development, becoming oocytes. (wikipedia.org)
  • Identification of nonfunctional PABPC1L causing oocyte maturation abnormalities and early embryonic arrest in female primary infertility. (cdc.gov)
  • 7) the developmental competence of porcine oocytes was significantly affected by an interaction among the age of the donor animal, ovarian follicle size, and exogenous gonadotropic stimulation. (uconn.edu)
  • Conclusions: This study compared the efficiencies of resveratrol, melatonin and their combination for protecting porcine oocytes from heat stress. (sagepub.com)
  • Concomitant with the progression of nuclear maturation, dynamic rearrangement of intracellular organelles promotes cytoplasmic maturation. (elifesciences.org)
  • Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. (tubitak.gov.tr)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • Results indicated that 12-hour caffeine pretreatment of canine COCs improves the MII maturation rates at 72 hours and BCB+ oocytes have higher competency invitro for nuclear maturation. (sruc.ac.uk)
  • The nuclear membranes surrounding fish and frog oocyte germinal vesicles (GVs) are supported by the lamina, an internal, mesh-like structure that consists of the protein lamin B3. (elsevierpure.com)
  • Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
  • The objective of this study was to determine the efficacy of leptin supplementation into maturation medium on nuclear maturation and fertilization rate of sheep oocytes. (ipb.ac.id)
  • At the end of maturation and fertilization processes, the oocytes were stained with 2% aceto orcein to determine nuclear maturation rate and pronuclear development. (ipb.ac.id)
  • 2004. Leptin enhances oocyte nuclear and cytoplasmic maturation via the mitogen-activated protein kinase pathway. (ipb.ac.id)
  • 2017. Insuline-Like groeth factor-I concentration in the follicular fluid of bali cattle and its role in the oocyte nuclear maturation and fertilization rate. (ipb.ac.id)
  • GH receptor is expressed in human oocytes and cumulus cells, [ 12 , 13 ] and GH has been shown to promote in vitro nuclear maturation of denuded human oocytes. (medscape.com)
  • This 31-year-old woman consulted our assisted reproduction center for a second opinion after having been advised, because of pervasive high serum follicle-stimulating hormone (FSH) levels, to pursue in vitro fertilization (IVF) with donor oocytes. (hal.science)
  • Conclusions Our data substantially expand the range of dysfunctional oocyte phenotypes incurred by mutation in TUBB8 , underscore the independent nature of human oocyte meiosis and differentiation, extend the class of genetic diseases known as the tubulinopathies and provide new criteria for the qualitative evaluation of meiosis II (MII) oocytes for in vitro fertilization (IVF). (bmj.com)
  • Prolactin concentration in follicular fluid during in vitro fertilization (IVF) correlates with the oocyte maturation level and fertilization rate. (medscape.com)
  • MIH activates the cytoplasmic maturation promoting factor (MPF), which is made up of two subunits: cyclin B (a regulatory subunit) and cdc2 (a catalytic subunit). (biomedcentral.com)
  • The signal of 1-MA is transduced into cytoplasmic formation of maturation-promoting factor (MPF) that eventually induces a germinal vesicle breakdown (GVBD). (elsevierpure.com)
  • These results indicate that in starfish oocyte membranes, 1-MA receptors are functionally coupled with the 39-kDa PTX-substrate G protein that transduces the signal into the formation of a cytoplasmic factor (MPF) and eventually into the reinitiation of meiosis. (elsevierpure.com)
  • It is believed to be the reason why the oocyte remains for so long in the immature dictyate state of meiosis. (wikipedia.org)
  • Aspiration of antral-stage follicles allowed the retrieval of 15 immature oocytes. (springer.com)
  • After oocyte collection, immature oocytes were cultured in the IVM medium. (springer.com)
  • Here, we show that injection of CL100 (also called MAPK phosphatase-1) into immature oocytes inhibited progesterone-induced oocyte maturation as well as MAPK activation and that injection of mRNA encoding a constitutively active MAPKK induced activation of histone H1 kinase and germinal vesicle breakdown in the absence of progesterone. (elsevierpure.com)
  • The increase in Mos protein and the activation of MAPK by injecting cyclin A protein into immature oocytes were both blocked also by cycloheximide treatment. (elsevierpure.com)
  • The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
  • However, the improved maturation rate of oocytes with leptin supplementation at a dose of 10 ng/mL could not improve the fertilization rate of the oocytes. (ipb.ac.id)
  • Evaluation of TUBB8 gene alterations in infertile women with oocyte maturation and cleavage arrest referred to Royan Institute. (cdc.gov)
  • Design and Study Population A total of 105 infertile women were enrolled in the randomized controlled trial: 70 women were with a history of RIF with donated oocytes, and 35 infertile women underwent the first oocyte donation attempt. (medscape.com)
  • Ovarian development in fish is broadly divided into two major phases: growth and maturation. (biomedcentral.com)
  • In experiment III, worms collected in spring were exposed to different temperature regimes to examine the effect of raising temperature on the synchronization of oocyte growth and maturation. (frontiersin.org)
  • The aims of this study were to investigate the effect of oviductal cells on in vitro maturation (IVM) of canine oocyte in Tissue Culture Medium 199 (TCM-199) or synthetic oviductal fluid (SOF) supplemented with bovine serum albumin (BSA) or fetal calf serum (FCS) and to compare the maturation rates of oocytes from the diestrus and anestrus stages. (tubitak.gov.tr)
  • It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
  • In the present study we investigated the effects of insulin exposure during in vitro bovine oocyte maturation on developmental rates, embryo quality and gene expression. (slu.se)
  • Cathepsin B inhibition improved bovine developmental competence of the oocytes, especially in the low-quality oocytes. (advetresearch.com)
  • In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
  • Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
  • In this study we evaluated the expression of CTSL during oocyte meiotic maturation and the effect of CTSL inhibition using specific inhibitor "SCP110" on the subsequent maturation process. (advetresearch.com)
  • Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
  • During oocyte growth, follicle stimulating hormone (FSH) stimulates production of estradiol-17β from the ovary. (biomedcentral.com)
  • Here, we report an original case of GROS, associated with compound heterozygous follicle-stimulating hormone receptor (FSHR) variants, in a woman who achieved a live birth by in vitro maturation (IVM) of her oocytes. (hal.science)
  • [ 14 ] In addition to its direct effect on the oocytes and/or cumulus cells, GH may also influence oocyte quality indirectly, through activation of insulin-like growth factor-I synthesis or promotion of follicle-stimulating hormone-induced ovarian steroidogenesis (reviewed in [ 15 ] ). (medscape.com)
  • Mitogen-activated protein kinase (MAPK) and MAPK kinase (MAPKK) are activated during Xenopus oocyte maturation concomitant with the activation of maturation promoting factor (MPF). (elsevierpure.com)
  • The results suggest that oocytes obtained with IVM in patients with ROS are capable of meiotic and mitotic division, fertilization, and generation of euploid embryos. (springer.com)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
  • [ 3-6 ] It is widely assumed that this improvement is related to the beneficial effects of GH on oocyte quality, as suggested by the observations of a higher number of oocytes collected, higher fertilization rate, and a higher number of embryos reaching the transfer stage in GH-treated patients. (medscape.com)
  • Resultant matured oocytes were fertilized by intracytoplasmic sperm injection (ICSI). (springer.com)
  • This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
  • Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation. (bvsalud.org)
  • Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single- cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. (bvsalud.org)
  • T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. (bvsalud.org)
  • The effect of oviductal cells on in vitro maturation of canine oocytes" by SİNEM ÖZLEM ENGİNLER, ASİYE İZEM SANDAL et al. (tubitak.gov.tr)
  • In this study, the effects of 12-hour caffeine pretreatment in a biphasic IVM protocol were assessed on maturation rates of canine oocytes. (sruc.ac.uk)
  • The oocyte maturation inhibitor (OMI) is an inhibitory factor created by follicular cells during a primary oocyte maturation. (wikipedia.org)
  • [2] It is developed from the primary oocyte by maturation. (wikipedia.org)
  • The thesis investigated the various factors influencing porcine in vitro maturation of an oocyte (IVM), fertilization (IVF), and early embryo culture (IVC). (uconn.edu)
  • The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. (nih.gov)
  • Background TUBB8 is a primate-specific β-tubulin isotype whose expression is confined to oocytes and the early embryo. (bmj.com)
  • To determine if the effect of TGF-beta1 on oocyte maturation involves transcription and/or translation, ovarian follicles were pre-treated with actinomycin D, a blocker of transcription, and cyclohexamide, an inhibitor of translation, and incubated with hCG or DHP, either alone or in combination with TGF-beta1 and oocyte maturation scored. (biomedcentral.com)
  • Normal maturation and growth of fullestrogenreceptoractivation.Silybin andfromthefirstdayofovarianstimula- oocytesdependsonadequatesupport B,oneofthecomponentsofsilymarin, tion,onegroupofpatientsreceivedgo- from ovarian granolusa cel s of the has(probable)weakestrogenreceptor nadotropinplussilymarin(70mg×3/ follicles [ 1 ]. (who.int)
  • Recent research showed that cathepsins family play an important role in affecting the oocytes developmental competence in many species. (advetresearch.com)
  • The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation , such as mammalian oocytes . (bvsalud.org)
  • Mammalian oocytes are maintained in meiotic prophase arrest for a very long time-months in mice, years in humans. (wikipedia.org)
  • The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte. (stowers.org)
  • Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
  • First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
  • Caffeine, a nonselective competitive PDE inhibitor, due to its structural similarity to adenosine molecule maintains the cAMP level by occupying PDE enzymes such as PDE-3A inside the oocyte and PDE-4 and PDE-5 in the cumulus cells. (sruc.ac.uk)
  • Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)
  • A critical issue for their mone secretion was caused by the abnormal expression of fertility failure is the poor quality of oocyte maturation. (sagepub.com)
  • 2010. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. (ipb.ac.id)
  • This results in the production of a secondary oocyte and one polar body. (wikibooks.org)
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
  • Following ovariohysterectomy, 13 pairs of ovaries were collected from bitches in anestrus (n = 10) or diestrus (n = 3) and oocytes were harvested by slicing. (tubitak.gov.tr)
  • The ovaries of a newborn baby girl contain about one million oocytes. (wikibooks.org)
  • When a young woman reaches puberty around age 10 to 13, a promary oocyte is discharged from one of the ovaries every 28 days. (wikibooks.org)
  • This protein is also involved in development of the ovaries, from before birth through adulthood, and is important for development and maintenance of egg cells (oocytes) in the ovaries. (medlineplus.gov)
  • In addition, its efficiency against oocyte had an adverse effect on their developmental com- HS will be compared with melatonin, another potent anti- petence by increasing GSH content and inhibiting the pro- oxidant, and the combination of melatonin with resveratrol. (sagepub.com)
  • In this study, we developed a heterogeneous microinjection system in which wild-type or mutated goldfish GV lamin B3 (GFLB3) was expressed in Escherichia coli, biotinylated, and microinjected into Xenopus oocytes. (elsevierpure.com)
  • GFLB3 that had been microinjected into Xenopus oocytes was also phosphorylated in Xenopus GV lamina, as judged by Western blotting with C7B8D. (elsevierpure.com)
  • To determine the effect of TGF-beta1 on mRNA levels of several key effectors of oocyte maturation, three sets of experiments were performed. (biomedcentral.com)
  • In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without oviductal cells, more positive effects were seen on canine oocyte maturation than with TCM-199 medium supplemented with same protein sources and oviductal cells. (tubitak.gov.tr)
  • In Caenorhabditis elegans, germline immortality involves proteostasis renewal at the beginning of each new generation, when oocyte maturation signals from sperm trigger the clearance of carbonylated proteins and protein aggregates. (elsevierpure.com)
  • Oocyte maturation signals are known to trigger protein-aggregate removal via lysosome acidification. (elsevierpure.com)
  • Indeed, plasma membrane preparations of A. pectinifera oocytes contained a G protein consisting of 39-kDa α, 37-kDa β, and 8-kDa γ subunits. (elsevierpure.com)
  • Injection of recombinant STE11 protein (a yeast MAPKK kinase) also induced initiation of oocyte maturation. (elsevierpure.com)
  • The former spot disappeared when the native lamina was incubated with lambda phage protein phosphatase (λ-PP), indicating that a portion of the lamin protein was already phosphorylated in the goldfish GV-stage oocytes. (elsevierpure.com)
  • The adenylyl cyclase is kept active by a constitutively active G-protein-coupled receptor known as GPR3 and a G-protein, Gs, also present in the oocyte membrane. (wikipedia.org)
  • Galvão A, Segers I, Smitz J, Tournaye H, De Vos M. In vitro maturation (IVM) of oocytes in patients with resistant ovary syndrome and in patients with repeated deficient oocyte maturation. (springer.com)
  • In conclusion, the supplementation of leptin at a dose of 10 ng/mL could increase the number of oocytes that reached MII stage, but could not increase the fertilization rate. (ipb.ac.id)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
  • Some mutations in oocytes lead to infertility in the mature organism, whereas others, over a long period of time, may eventually lead to the emergence of novel species or subspecies. (elifesciences.org)
  • One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)
  • The maturation process was conducted using a tissue culture medium (TCM) 199 with four supplementation treatments of leptin namely 0 (control), 10, 50, and 100 ng/mL. (ipb.ac.id)
  • For example, oocytes harbouring any of three missense mutations (I210V, T238M and N348S) could extrude the first polar body. (bmj.com)
  • ScholarWorks@Gyeongsang National University: Coculturing cumulus oocyte complexes with denuded oocytes during in vitro maturation affects oocyte maturation, zona hardening, polyspermy and oocyte GPX 1 gene expression. (gnu.ac.kr)
  • We previously found that mutations in TUBB8 caused oocyte maturation arrest. (bmj.com)
  • Methods and results Patients with oocyte maturation arrest were sequenced with respect to TUBB8 . (bmj.com)
  • Surprisingly, oocytes from patients harbouring homozygous TUBB8 mutations that in either case preclude the expression of a functional TUBB8 polypeptide nonetheless contained identifiable spindles. (bmj.com)
  • We investigated the effects of identified mutations in vitro, in cultured cells and in mouse oocytes. (bmj.com)
  • These mutations cause a range of folding defects in vitro, different degrees of microtubule disruption upon expression in cultured cells and interfere to varying extents in the proper assembly of the meiotic spindle in mouse oocytes. (bmj.com)
  • HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
  • In the granulosa cells, under the action of 20β-hydroxysteroid dehydrogenase (20β-HSD), 17α-HP is converted to 17α, 20β-dihydroxyprogesterone (DHP), the maturation inducing hormone (MIH) in cyprinids, such as zebrafish and goldfish. (biomedcentral.com)
  • The granulosa cells produce a small molecule, cyclic GMP , that diffuses into the oocyte through the gap junctions. (wikipedia.org)
  • Oocyte maturation in teleosts is triggered by the release of leutinizing hormone (LH) from the pituitary. (biomedcentral.com)
  • In conclusion, using two unique genetic models, our study demonstrates the endogenously synthesized DHA promotes oocyte maturation and oocyte quality by promoting pregnenolone production via transcriptional regulation of cyp11a1. (zoores.ac.cn)
  • We report the pregnancy and live birth achieved after in vitro maturation (IVM) of oocytes and PGT-A in a 23-year-old patient suffering from ovarian gonadotropin resistance. (springer.com)
  • Without gonadotropin priming and HCG trigger, ultrasound-guided transvaginal oocyte retrieval was performed. (springer.com)
  • Recently, we showed that TGF-beta1 inhibits gonadotropin- and 17alpha, 20beta-dihydroxyprogesterone (DHP)-induced maturation in zebrafish. (biomedcentral.com)
  • These data suggest that administration of silymarin in IVF patients concomitantly with gonadotropin results in reduction of granolusa cell apoptosis but does not have any effect in promotion of follicular development, oocyte retrieval or endometrial thickness. (who.int)
  • Oogenesis starts with the process of developing primary oocytes, which occurs via the transformation of oogonia into primary oocytes , a process called oocytogenesis . (wikipedia.org)
  • PTX-inhibition of GVBD was rescued by the injection of MPF into PTX-preinjected oocytes. (elsevierpure.com)
  • Moreover, CTSL inhibition reduced the maturation rate compared to the control group as we found a significant decrease in PBE percent. (advetresearch.com)
  • Finally, we evaluated matured oocytes quality after CTSL inhibition, importantly, aneuploidy incidence was increased significantly after CTSL inhibition. (advetresearch.com)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • Taken together, our results suggest that the balance between phosphorylation by interphase lamin kinases and dephosphorylation by phosphatases regulates the conformational changes in the lamin B3 N-terminal head domain that in turn regulates the continual in vivo rearrangement and remodeling of the oocyte lamina. (elsevierpure.com)