AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
VitrificationCryopreservationCryoprotective AgentsFertility PreservationOocytesEthylene GlycolPropylene GlycolFreezingOocyte RetrievalFertilization in VitroReproductive Techniques, AssistedEmbryo TransferBlastocystEmbryo Culture TechniquesPregnancy RateSemen PreservationTissue PreservationDimethyl SulfoxideSucroseSperm Injections, IntracytoplasmicInfertilityInfertility, FemalePregnancyEmbryonic DevelopmentOvulation InductionCell SurvivalMorulaThermal ConductivityFreeze SubstitutionEmbryo, MammalianPropylene GlycolsTissue SurvivalOvaryCleavage Stage, OvumEmbryo ImplantationEthylene GlycolsOocyte DonationSpermatozoaIn Vitro Oocyte Maturation TechniquesTrehaloseOvarian FollicleOogenesisPregnancy OutcomeSperm MotilityIceDry IceTissue BanksRewarmingFertilizationOrgan PreservationMetaphaseMice, Inbred ICRZona PellucidaLive BirthAsepsisGlycerolZygoteCumulus CellsTissue Culture TechniquesTime FactorsButylene GlycolsHEPESParthenogenesisTreatment OutcomeBlastomeresSingle Embryo TransferSperm BanksSus scrofaFetal ViabilityEmbryonic and Fetal DevelopmentEctogenesisMeiosisAge FactorsFertilitySolutionsAquaporin 3NitrogenCold TemperatureOutcome Assessment (Health Care)Mice, Inbred CBACattleCulture TechniquesCell Culture TechniquesBlood PreservationFormamidesFicollSpecimen HandlingOsmosisSemen AnalysisHot TemperatureMicromanipulationPlasticsXenopus laevisTemperaturePachytene StageSperm Retrieval
EmbryosFertility preservationHuman oocytesVitro fertilizationSpermMaturationRetrievalImmature oocytesCentersTechniques of vitrificationProduction and cryopreservationBlastocystsBiopsyDonor oocytesICSIPregnancy outcomesFormation of ice crystalsPronuclearFertilizationCyclesMetaphasePoor IVF outcomesWoman'sFrozen embryoFreezeFetalMethodSurvival ratesSemenVitrifyConcentrationsVersusBovineGeneticFreezingSignificantlyDonorsBlastocystCryoprotectantsGametesWomenProcessSuccessfully appliedRoutineSpecimensFactorsLiquid nitrogenCattleThawEggs FrozenRatePatientsAdultAdvancesDecline
Embryos27
  • Laboratory advances such as biopsy of the trophectoderm (the outer cell layer of a day 5 embryo) and vitrification (a method of rapid cooling of embryos that minimizes ice crystal formation) have further improved success. (pacificfertilitycenter.com)
  • Of the 1772 oocytes rewarmed, 88% survived, 75% fertilized, and 51% resulted in viable cleavage stage (day 3) embryos. (pacificfertilitycenter.com)
  • Oocyte cryopreservation is an option for individuals undergoing IVF who object, either for religious or ethical reasons, to the practice of freezing embryos. (wikipedia.org)
  • Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)
  • The process of preserving an embryo at an embryogenesis stage agreeing to pre-implantation is called Cryopreservation of embryos. (acimc.org)
  • If more oocytes are retrieved or embryos are formed then they can be used for donation to another woman or a couple or they can be frozen and used specifically. (acimc.org)
  • Tony's studies focused on cryopreservation and non-invasive methods of predicting euploidy in early embryos. (ivfmeeting.com)
  • His studies included comparison of cryopreservation methods of oocytes and pre-implantation genetic screening (PGS) of embryos for optimized outcomes. (ivfmeeting.com)
  • 2012) A Critical Appraisal of Cryopreservation( Slow Cooling versus Vitrification) of Human Oocytes and Embryos. (impeckoble.com)
  • Exclusion criteria: 1) OC for a medical reason, as research, due to lack of sperm or a natural disaster, combined with embryos or for gestational carrier use, or 2) AOs/embryos from OC transported out before a LB. Primary outcome was CLBR (LB + OP). Patients (pts) were stratified by age and #AOs or metaphase II oocytes (M2s) thawed. (nyu.edu)
  • The primary outcome was number of euploid embryos. (nyu.edu)
  • Secondary outcomes were cycle start follicle stimulation hormone level (FSH), total gonadotrophin (GND) dose, number oocytes, mature oocytes (MII), fertilization rate (2PN), and number of embryos biopsied (BX). (nyu.edu)
  • Students will freeze, thaw and analyse sperm samples, oocytes and embryos Students will gain a better understanding of media components by making and designing their own media. (monash.edu)
  • Military members who wish to preserve their fertility before deployment often will freeze sperm, oocytes, or embryos to safeguard their options for future family building. (reprotech.com)
  • Because gender-affirming hormone replacement therapy (HRT) has the possibility to negatively impact future fertility, many trans people choose to preserve and protect their fertility before beginning HRT and will work with a fertility clinic or sperm bank to freeze oocytes, embryos, or sperm for future family building. (reprotech.com)
  • Embryo cryopreservation, also known as embryo freezing, is the freezing and storage of embryos for future use. (icloudhospital.com)
  • Embryo cryopreservation entails in vitro fertilization , a method in which eggs are extracted from a woman's ovary and joined with sperm in a laboratory to generate embryos. (icloudhospital.com)
  • Other times, embryo cryopreservation allows women who are receiving medical procedures that may compromise fertility to freeze embryos and use them later. (icloudhospital.com)
  • Cryotec is an innovative method and product line developed by Dr. Masashige Kuwayama and REPROLIFE Inc. for the vitrification and warming of oocytes and embryos. (reprolife.jp)
  • this means that oocytes and embryos of all stages can be treated using the same protocol, leading to greater efficiency and convenience. (reprolife.jp)
  • Cryotec is a safe and effective method even for lower-grade oocytes and embryos that would be difficult to vitrify using traditional methods. (reprolife.jp)
  • Cryotec can be used to safely vitrify and warm oocytes and embryos that are sensitive due to the patient's age, constitution, or medical conditions. (reprolife.jp)
  • His advancements in solution composition, device design, and simplified protocols significantly improve success rates for oocytes and embryos. (reprolife.jp)
  • Hands-on sessions are focused on all steps needed to implement successfully a PGT program, including: vitrification and warming of biopsied embryos, laser assisted hatching, tubing of cells for genetic analysis and validation of the number of cells biopsied. (embryotools.com)
  • Dr. Henri Woelders, of Wageningen Livestock Research (WLR) and the Centre for Genetic Resources, the Netherlands (CGN) presented on the potential of cryopreservation and use of various types of germplasm in pigs and chicken, focussing on embryos, gonads, primordial germ cells and semen, and including some highlights from the EU Horizon 2020 IMAGE project (LINK). (wur.nl)
  • Utrecht can do ovum pick-up from mares and collaborate with Avantea, Italy for in vitro production and cryopreservation of embryos. (wur.nl)
  • The Evaluation of Various Conditions in the Cryopreservation of Mouse Embryos - Rapid and Slow Method of Cryopreservation, Culture Media and Cell Stages. (ecerm.org)
Fertility preservation8
Human oocytes2
Vitro fertilization4
  • The egg retrieval process for oocyte cryopreservation is the same as that for in vitro fertilization. (wikipedia.org)
  • 11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)
  • Evidence-based outcomes after oocyte cryopreservation for donor oocyte in vitro fertilization and planned oocyte cryopreservation: a guideline. (nyu.edu)
  • Effect of a Baseline Ovarian Cyst on the Outcome of in Vitro Fertilization-embryo Transfer. (ecerm.org)
Sperm10
  • Semen cryopreservation (commonly called sperm banking or sperm freezing ) is a procedure to preserve sperm cells. (iiab.me)
  • Treatment of sperm with heparin binding proteins prior to cryopreservation showed decreased cryoinjury and generation of ROS. (iiab.me)
  • Some evidence suggests an increase in single-strand breaks, condensation and fragmentation of DNA in sperm after cryopreservation. (iiab.me)
  • Scientists have tried for years to save those eggs before they dwindle, but while sperm has been successfully frozen for more than 60 years, the "experimental" label was lifted from oocyte cryopreservation by the American Society for Reproductive Medicine (ASRM) only last October, thanks to a new flash-freezing process called vitrification. (washingtonian.com)
  • Abnormalities in sperm DNA prevent the introduction of damaged DNA into the oocyte. (reprotech.com)
  • Some programs also offer ovarian tissue or testicular tissue cryopreservation for adolescents who meet certain criteria and do not wish to do ovarian stimulation for oocyte collection or produce a sperm sample. (reprotech.com)
  • Because of the relatively high cost and high success rate of sperm cryopreservation options for men, embryo cryopreservation is reserved for use in maintaining fertility in women before or after cancer. (icloudhospital.com)
  • Two day course based on different types of oocyte injection, sperm capture and immobilization, oocyte rotation, IMSI and PICSI. (embryotools.com)
  • Dr. Bernard Roelen of the Faculty of Veterinary Medicine, Utrecht University, explained what can be expected from production of sperm and oocytes in vitro, on the basis of the latest international scientific progress in mice. (wur.nl)
  • Embryonic stem cells (ESCs) from the 'inner cell mass' of an early embryo give rise to all future cells and tissue of an animal, including the primordial germ cells, which ultimately form the germ cells (sperm or oocytes). (wur.nl)
Maturation5
  • Immature oocytes have been grown until maturation in vitro, but it is not yet clinically available. (wikipedia.org)
  • Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
  • The protocol include all the procedures from in vitro maturation, vitrification, warming, and post-warming culture. (jove.com)
  • After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
  • Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
Retrieval5
Immature oocytes1
  • Illustrative example of progress made is the world-first foal born in 2017 after development of an improved vitrification protocol for equine immature oocytes. (wur.nl)
Centers2
Techniques of vitrification1
  • As we further perfect our own techniques of vitrification, we will be increasingly more confident in our ability to offer young women with a viable option for future family planning in addition to embryo freezing and donor gametes. (pacificfertilitycenter.com)
Production and cryopreservation1
  • The theoretical aspects of IVF, embryo production and cryopreservation will be covered in the previous unit reinforced by extensive practical training The theoretical knowledge gained in slow cooling and vitrification techniques will also enhanced by practical training. (monash.edu)
Blastocysts3
Biopsy1
  • Dr. Charlene Alouf gave an impressive talk with unique insight through many publications on whether to Biopsy or re-biopsy following vitrification. (ivfmeeting.com)
Donor oocytes7
  • Improvements in oocyte cryopreservation techniques and establishment of cryopreserved donor oocyte banks have led to improved access to and lower cost of donor oocytes, upending the traditional practice of fresh oocyte donation. (biomedcentral.com)
  • The objective of this study was to examine national trends in utilization and live birth rates with fresh versus cryopreserved donor oocytes. (biomedcentral.com)
  • A retrospective analysis of 2013 through 2015 aggregate U.S. national data reported by the Society for Assisted Reproductive Technology which included 30,160 IVF cycles with either fresh or cryopreserved donor oocytes was performed. (biomedcentral.com)
  • Over the three-year study period fresh donor oocytes produced stable live birth rates per recipient cycle start while those with cryopreserved oocytes significantly declined year-by-year. (biomedcentral.com)
  • Despite rising popularity of cryopreserved donor oocytes, prospective patients should be counselled that fresh donor oocytes still represent standard of care due to higher live birth rates. (biomedcentral.com)
  • Though ASRM recommended against routine donor oocyte banking until more robust clinical data on safety and efficacy of cryopreserved donor oocytes were generated, multiple commercial cryopreserved oocyte donor banks were nevertheless concurrently established in the U.S. Based on 2013 U.S. national data, we previously reported that live birth rates for recipients of donated cryopreserved oocytes were lower than those of fresh oocytes [ 2 ]. (biomedcentral.com)
  • Figure 1 demonstrates that live birth rates per recipient cycle start were higher with fresh donor oocytes in each of the studied years. (biomedcentral.com)
ICSI1
Pregnancy outcomes5
  • To investigate whether there was a difference in pregnancy outcomes between modified natural cycle frozen-thawed embryo transfer (NC-FET) cycles and artificial cycles (AC)-FET in women who all had regular menstrual cycles. (tjoddergisi.org)
  • These findings suggest that although both FET protocols are equally effective in terms of pregnancy outcomes in women with regular menstrual cycles, NC-FET is more favorable because it requires no medication, has no adverse events, and has a significant cost reduction. (tjoddergisi.org)
  • However, some controversy exists because some investigators reported better pregnancy outcomes with AC-FET, (1,2,5,13) whereas the results of some retrospective studies demonstrated the superiority of NC-FET (14,15) . (tjoddergisi.org)
  • This single-centre, large-sample retrospective trial was designed to investigate the contribution of strengthened LPS to pregnancy outcomes for groups with low progesterone levels on the FET day in artificial endometrium preparation cycles. (biomedcentral.com)
  • Women who are not ready to become pregnant now but who want to optimize their pregnancy outcomes later should consider freezing their eggs. (sbivf.com)
Formation of ice crystals1
  • Vitrification, the most commonly used method, involves rapid freezing, reducing the formation of ice crystals and potential damage to the eggs. (parkwayeast.com.sg)
Pronuclear1
  • Vitrification has also become the method of choice for pronuclear oocytes, although prospective randomized controlled trials are still lacking. (wikipedia.org)
Fertilization1
  • Fertilization and in vitro Development of Bovine Oocytes Following Round Spermatid Injection. (ecerm.org)
Cycles7
  • A 5-10% live birth rate per oocyte in women under the age of 35 years translates to a respectable chance of having a baby in the future from one to two treatment cycles in the present (10-20 oocytes can be expected to be cryopreserved per cycle). (pacificfertilitycenter.com)
  • The chemical, clinical, and ongoing pregnancy rates, miscarriage and live birth rates were compared in these two distinct FET cycles to identify predictive factors and to influence the reproductive outcome. (tjoddergisi.org)
  • While it is true that obtaining a maximum number of 15-20 oocytes for vitrification (probably in two cycles of stimulation) is relatively easy in young women, this may be more difficult in a patient with a compromised ovarian reserve, which may even be aggravated if the patient has undergone surgery", said Dr. Cobo. (ivi-fertility.com)
  • method: 77% vitrification, 4% slow cooling, 19% both), 604 thaw and 465 transfer cycles. (nyu.edu)
  • The results indicated that the serum progesterone level on the FET day was one of the risk factors predicting the chances of pregnancy in artificial endometrium preparation cycles, and strengthened LPS in patients with low progesterone on the FET day might help to provide a reasonable pregnancy outcome in artificial cycles, although further prospective evidence is needed to confirm this possibility. (biomedcentral.com)
  • Shady Grove Fertility, whose network of clinics span DC, Maryland, and Virginia, performed 131 cryopreservation cycles for women banking their own eggs in 2012-up from 44 in 2010. (washingtonian.com)
  • Yildiz S, Turkgeldi E, Kalafat E, Keles I, Gokyer D, Ata B. Do live birth rate and obstetric outcomes vary between immediate and delayed embryo transfers following freeze-all cycles? (artfertilityclinics.com)
Metaphase1
  • Vitrification is associated with higher survival rates and enhanced development compared to slow-cooling when applied to oocytes in metaphase II (MII). (wikipedia.org)
Poor IVF outcomes1
  • Given that omega-3 is essential for embryo development at a cell signalling level, it could contribute to the reason behind poor IVF outcomes in many obese women. (fertilityfirst.com.au)
Woman's1
Frozen embryo2
  • In fact, pregnancy rates are increased following frozen embryo transfer, and perinatal outcomes are less affected, compared to embryo transfer in the same cycle as ovarian hyper stimulation was performed. (acimc.org)
  • However, in the frozen embryo transfers, there were no significant differences in clinical outcomes between the two groups. (ecerm.org)
Freeze2
Fetal2
  • The progressive loss of oocytes from fetal life through menopause is a normal process. (ldysinger.com)
  • Until now, follow-up studies concerning fetal health after cryopreservation have not been available in sufficient number and results are still awaited. (uni-luebeck.de)
Method10
Survival rates3
  • This review presents some of the most noticeable aspects related with the oocyte cryopreservation procedures, emphasizing their evolution in the bovine, which points towards the critical points determining the reduced survival rates of female gametes to freezing and vitrification. (camkkinases.com)
  • It features simplified protocols, embryologist-friendly product designs, and focuses on achieving high survival rates in cryopreservation. (reprolife.jp)
  • His unwavering dedication drives innovation for high survival rates and stress-free vitrification for embryologists. (reprolife.jp)
Semen2
  • Semen can be used successfully indefinitely after cryopreservation. (iiab.me)
  • He showed that cryopreservation of poultry semen is applicable for long term conservation of rare breeds, and may also be used for maintaining breeding lines. (wur.nl)
Vitrify2
Concentrations3
  • Vitrification is much faster but requires higher concentrations of cryoprotectants to be added. (wikipedia.org)
  • The researchers analysed the fatty acid concentrations in the follicular fluid surrounding the growing oocyte and discovered that women who were obese had lower levels of saturated fatty acids and polyunsaturated omega-3 fatty acids. (fertilityfirst.com.au)
  • Another factor to consider is the impact fatty acids can have during oocyte and embryo cryopreservation, high lipid concentrations contributing to cryodamage. (fertilityfirst.com.au)
Versus1
Bovine1
Genetic2
  • However, it is also possible that such genetic defects result in a smaller complement of oocytes during gonadal development. (ldysinger.com)
  • The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species conservation, and increase the research in biotechnology. (jove.com)
Freezing10
Significantly2
  • Vitrification significantly minimized that damage so that thaw rates are almost identical to using fresh eggs. (washingtonian.com)
  • To test this hypothesis, we here report two additional years of national outcome data that have become available since our original publication, demonstrating that the par between fresh and cryopreserved oocyte over these 2 years has actually significantly widened. (biomedcentral.com)
Donors2
Blastocyst1
  • Collected MII oocytes were vitrified and divided into four groups: untreated, 10 mM caffeine (CA), 10 μM MG132 (MG), and 10 mM caffeine +10 μM MG132 (CA+MG). After warming, the MPF activity of oocytes and their blastocyst formation and implantation rates in the CA, MG, and CA+MG groups were much higher than those in the untreated group. (molcells.org)
Cryoprotectants1
  • Cryopreservation causes the cessation of cellular activity through the removal of water and the addition of cryoprotectants. (reprotech.com)
Gametes1
  • Oocyte vitrification (cryopreservation) has the potential to rescue gametes from deceased animals, it would make oocyte banking for research possible, and superior female genetics could be cryopreserved. (wur.nl)
Women4
  • however, this initial study has revealed a reasonable explanation for why many women with high BMIs may see adverse outcomes when undergoing IVF treatment. (fertilityfirst.com.au)
  • In normal women, at approximately 37.5 years of age, an accelerated atresia of the oocytes begins 1 , 2 ( Figure 1 ). (ldysinger.com)
  • In this regard, a previous study, also led by Dr. Cobo, showed that young women achieved better outcomes when they had oocytes vitrified to preserve fertility prior to surgical removal of the ovarian endometrioma. (ivi-fertility.com)
  • Recognizing the need for safer oocyte cryopreservation, Dr. Kuwayama provides opportunities for women facing age-related infertility and those undergoing cancer treatment. (reprolife.jp)
Process3
  • The expertise and experience of the medical professionals overseeing the process can greatly impact the outcome. (a4fertility.com)
  • What happens before Embryo Cryopreservation process? (icloudhospital.com)
  • Prof. Tom Stout of the Faculty of Veterinary Medicine, Utrecht University, explained in detail the process of in vitro production (IVP) and cryopreservation and transfer of horse embryo's. (wur.nl)
Successfully applied2
  • Vitrification has been developed and successfully applied in IVF treatment with the first live birth following vitrification of oocytes achieved in 1999. (wikipedia.org)
  • Cryopreservation of mammalian oocytes has been successfully applied for long-term storage of surplus eggs in mammalian assisted reproductive technology (ART), including in humans. (molcells.org)
Routine2
  • Although the first establishment of pregnancy by using a cryopreserved (frozen) oocyte was reported in 1986, the routine use of cryopreserved oocytes for achieving pregnancy became possible later after a long time. (bebekistiyorum.com)
  • Three day course for those embryologists who are new in vitrification or for those who are already performing vitrification in their daily routine, and even thought they just want to update their knowledge and learn some tips and tricks to improve results. (embryotools.com)
Specimens1
  • 1 The images in Panel B - which shows histologic specimens of oocytes (arrows) taken from patients at birth, at the age of 25 years, and at the age of 50 years - are adapted from Erickson 8 and are reprinted with the permission of the publisher. (ldysinger.com)
Factors2
  • Thus, intrinsic factors governing the developmental potential that diminish during oocyte cryopreservation should be explored. (molcells.org)
  • It is clear that the number of vitrified oocytes and the age of the patient are key factors for reproductive success. (ivi-fertility.com)
Liquid nitrogen1
Cattle3
  • Vitrifaction by ultrarapid cooling and warming is considered a standard approach in cattle oocyte preservation. (jove.com)
  • Prof. Ann van Soom of the Faculty of Veterinary Medicine, Ghent University, presented an overview of the major progress made in the past years in vitrification, oocyte and embryo culture in horses, cats and cattle. (wur.nl)
  • Human oocyte vitrification works very well, further work on oocyte vitrification in cattle, horse, cat can learn from human. (wur.nl)
Thaw1
  • This is the largest report to date of AO thaw outcomes from a single U.S. center. (nyu.edu)
Eggs Frozen1
  • According to ASRM, the 1,000-plus reported births from eggs frozen via vitrification show no increased risk of birth defects compared to those conceived naturally. (washingtonian.com)
Rate5
  • The main studied outcome measure was the clinical pregnancy rate per cycle. (tjoddergisi.org)
  • As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)
  • Vitrification, an alternative cryopreservation tool, has recently been shown to prevent ice formation and involves the use of a high concentration of CPAs and extremely high cooling rate. (molcells.org)
  • Thus, in patients under 35 years of age with endometriosis, a 95% success rate was achieved, retrieving approximately 20 oocytes, while the maximum new-born rate was close to 80% in patients over 35 years of age. (ivi-fertility.com)
  • The primary endpoint was the live birth rate, and the secondary endpoints were clinical pregnancy, miscarriage and neonatal outcomes. (biomedcentral.com)
Patients1
  • This research aims to help fertility specialists and endometriosis patients set realistic expectations about their chances of reproductive success based on their vitrified oocytes", explained Dr. Cobo. (ivi-fertility.com)
Adult1
Advances1
  • Also, recent advances in pig embryo cryopreservation were shown and it was demonstrated how mathematic modelling of cellular events during embryo cryopreservation are extremely helpful. (wur.nl)
Decline1