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  • gene
  • We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptospordium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. (eurekamag.com)
  • Toxoplasma
  • Oocyst stage of Toxoplasma gondii is characterized by a durable wall that confers a strong protection to this protozoan parasite in face of harsh environmental conditions. (blogspot.com)
  • protein
  • We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptospordium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. (eurekamag.com)
  • excystation
  • These observations not only highlight the risk that aquatic biofilms pose in regards to Cryptosporidium outbreaks from water distribution systems, but further indicate that even simple biofilms are able to stimulate oocyst excystation and support the extracellular multiplication and development of Cryptosporidium within aquatic environments. (edu.au)
  • Giardia
  • Recipient(s) will receive an email with a link to 'Total and infectious Cryptosporidium oocyst and total Giardia cyst concentrations from distinct agricultural and urban contamination sources in Eastern Canada' and will not need an account to access the content. (iwaponline.com)
  • Specificity
  • SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was analyzed. (asm.org)
  • Specificity to wall of T. gondii oocyst was confirmed and revealed absence of cross reactivity with bradyzoite cyst wall and tachyzoites. (blogspot.com)
  • inactivation
  • Experiments conducted on days with different levels of solar insolation identified rapid inactivation of oocysts in tap water (up to 90% inactivation within the first hour). (semanticscholar.org)
  • capsule
  • Laminin was also detected on the outer surface of the oocyst capsule, incorporated within the capsule and associated with sporozoites forming within the oocysts. (biomedcentral.com)
  • Thereafter, laminin secreted by midgut epithelial cells and/or haemocytes, binds to the outer surface of the oocyst capsule and that some passes through and is incorporated into the developing oocysts. (biomedcentral.com)
  • wall
  • Oocysts had a rough outer wall with apparent random depressions. (semanticscholar.org)
  • The oocyst wall is composed of 2 layers: an osmiophilic outer layer consisting of a rough external and smooth internal surface, and a relatively thick, electron-lucent inner layer. (semanticscholar.org)
  • microscopy
  • These microscopy observations were further supported by flow cytometric analyses, where excysted oocyst populations were detected in 1, 3 and 6 day-old Cryptosporidium-exposed biofilms, but not in biofilm-free controls. (edu.au)
  • strain
  • Ten 36-day-old chickens were separated into two groups: control and experimental, orally inoculated with oocysts of the T. gondii strain M7741 genotype III. (scielo.br)
  • interactions
  • Genes differentially regulated during the ookinete-to-oocyst transition may determine the development of the parasite within the mosquito host, as well as, participating directly in parasite/mosquito interactions. (imperial.ac.uk)