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  • Tumor
  • It binds tumor necrosis factor (TNF)-receptor-associated factors (TRAFs), activates NFkappaB and triggers the transcription factor activating protein-1 (AP-1) via the c-Jun N-terminal kinase (JNK) cascade, but its specific contribution to AP-1 has not been elucidated fully. (biomedsearch.com)
  • kinase
  • Abstract -The aim of this study was to elucidate the upstream signaling mechanism that mediates the fluid shear stress activation of mitogen-activated protein kinases (MAPKs), including c-Jun NH 2 -terminal kinase (JNK) and extracellular signal-regulated kinases (ERKs), in vascular endothelial cells (ECs). (ahajournals.org)
  • Protein kinase A inhibits lysophosphatidic acid induction of serum response factor via alterations i. (biomedsearch.com)
  • mitogen-activat
  • Upon binding to their receptors, peptide growth factors initiate a cascade of signaling events which leads to the activation of cellular Ras, which in turn functions as a molecular switch to transmit mitogenic signals to a cascade of cytoplasmic kinases ( 35 ), ultimately resulting in the activation of mitogen-activated protein kinases (MAPKs) ( 20 ). (asm.org)
  • Genotype
  • B) Heatmap representation of inter-genotype genetic distances based upon external surface loops only or remaining (non-loop) backbone for the L1 proteins of the indicated Alpha-7 and Alpha-9 genotypes. (nih.gov)
  • cellular
  • Homogeneously staining chromosomal regions contain amplified copies of an abundantly expressed cellular oncogene (c-myc) in malignant neuroendocrine cells from a human colon carcinoma. (abnova.com)
  • The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. (abnova.com)
  • The interaction and function between c-Jun protein and Jun B protein increase the repertoire of possible regulatory complexes by LMP1 that could play an important role in the regulation of transcription of specific cellular genes in the process of genesis of nasopharyngeal carcinoma. (biomedsearch.com)
  • nitric oxide sy
  • Previous studies have shown that shear stress induces several genes that encode proteins implicated in atherosclerosis and other vascular diseases, including MCP-1, intercellular adhesion molecule-1, heparin-binding EGF-like growth factor, PDGF, nitric oxide synthase, and c- fos proto-oncogene. (ahajournals.org)
  • genes
  • Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. (abnova.com)
  • replication
  • The integrated subgenomic HPV fragments express viral oncoproteins and carry an origin of DNA replication that is capable of initiating bidirectional DNA re-replication in the presence of HPV replication proteins E1 and E2, which ultimately leads to rearrangements within the locus of the integrated viral DNA. (nih.gov)
  • We also confirmed that the replicating HPV episomes that expressed the physiological levels of viral replication proteins could induce genomic instability in the cells with integrated HPV. (nih.gov)
  • We detected the same pattern of co-localization of E1 and the other replication proteins as earlier, which once more demonstrates that these foci are active DNA replication initiation centers of the integrated HPV. (nih.gov)
  • This is supported by the fact that the E2 protein is not required for the unwinding or the helicase activity of E1 during DNA replication elongation and, likewise, the fact that polα is not required for DNA repair processes. (nih.gov)
  • The percentage of the E1/E2 positive cells with the characteristic pattern of viral DNA replication was estimated to be 5% to 10%, which represents a fairly large fraction of cells in S-phase within the transfected cell population. (nih.gov)
  • induce
  • Overexpression of the E2 protein in cervical cancer cells can induce growth arrest and/or apoptotic cell death, suggesting that E2 might be useful in the treatment of this disease. (openrepository.com)
  • VP22-E2 fusion proteins induce apoptosis in transiently transfected HPV-transformed cervical carcinoma cell lines. (openrepository.com)
  • VP22-E2 proteins produced in bacterial cells can also enter cervical cancer cells and induce apoptosis in a dose-dependent manner. (openrepository.com)
  • Retinoblastoma Protein
  • For example, passage through G 1 phase in quiescent and proliferating cells exhibits several important molecular differences, including expression of the cyclin-inhibitory protein p27 kip1 ( 3 ), Cdc6 ( 42 ), and Fos and related proteins ( 14 ), and formation of a complex between E2F and the retinoblastoma protein (Rb)-related p130 ( 19 , 34 ). (asm.org)
  • transactivation
  • We found that although different types of E2 proteins all exhibited transactivation and repression activities, depending on the sequence context of the E2-binding sites, HPV-16 E2 shows stronger transcription activity and greater DNA-binding affinity than those displayed by the other E2 proteins. (psjhealth.org)
  • MAPK
  • Thus, p60src plays a critical role in the shear stress activation of MAPK pathways and induction of Activating Protein-1 (AP-1)/TRE and Elk-1/SRE-mediated transcription in ECs. (ahajournals.org)
  • genotypes
  • Phylogenetic relationship between the major capsid proteins of the Alpha-7 and Alpha-9 genotypes. (nih.gov)
  • A) Amino acid sequences of the L1 major capsid proteins representing both VLP and pseudoviruses of the Alpha-7 (HPV18, HPV39, HPV45, HPV59, HPV68) and Alpha-9 (HPV16, HPV31, HPV33, HPV35, HPV52, HPV58) genotypes and the control BPV . (nih.gov)
  • A phylogenetic representation of the amino acid sequences used for the Alpha-7 and Alpha-9 VLP and pseudovirus L1 proteins demonstrates the close relationship between certain genotypes within each of these two species groups (Fig. 3A). (nih.gov)
  • differentially
  • To investigate whether E2 proteins encoded by high risk HPVs may function differentially from E2 proteins encoded by low risk HPVs and animal papillomaviruses, we conducted comparative DNA-binding and transcription studies using electrophoretic mobility shift assays and cell-free transcription systems reconstituted with purified general transcription factors, cofactor, RNA polymerase II, and with E2 proteins encoded by HPV-16, HPV-18, HPV-11, and bovine papillomavirus type 1 (BPV-1). (psjhealth.org)
  • It was also shown that shear stress differentially regulates JNK and ERK by signaling that involves PTx-insensitive G protein-dependent and G i2 -dependent pathways, respectively. (ahajournals.org)
  • Transfection
  • Transfection with a plasmid in which E6 and E7 were the only intact open reading frames (ORFs) induced an indefinite life-span in the keratinocytes with an efficiency similar to that of the entire early region of the viral DNA. (nih.gov)
  • The E6 and E7 proteins were detected in the lines induced by the E6E7 DNA and by co-transfection of the E6 and E7 plasmids. (nih.gov)
  • Transfection studies suggest that JNK interaction protein (JIP) could inhibit the heterodimer form of c-Jun and Jun B through blocking the AP-1 signaling pathway triggered by LMP1. (biomedsearch.com)
  • polymerase
  • Co-immunostaining of HPV18 E1 (Alexa Fluor 568, second column) together with the following proteins are shown: HPV18 E2, PCNA, RPA, polymerase α/primase, and Daxx (Alexa Fluor 488, first column). (nih.gov)
  • Amino
  • Interestingly, in quiescent cells the restriction point is identical to the G 1 arrest point induced by other conditions, including protein synthesis inhibitors and amino acid deprivation ( 29 ). (asm.org)
  • synthesis
  • The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. (abnova.com)
  • milk
  • Milk provides substantial amounts of tryptophan easily hydrolyzed from α-lactalbumin in milk´s whey protein fraction. (biomedcentral.com)
  • binds
  • Mitotic kinesin-like protein 2 binds and colocalizes with papillomavir" by Ting Yu, Yu-Cai Peng et al. (umassmed.edu)
  • closely related
  • Our studies thus categorize HPV-18 E2 and BPV-1 E2 in the same protein family, a finding consistent with the available E2 structural data that separate the closely related HPV-16 and HPV-18 E2 proteins but classify together the more divergent BPV-1 and HPV-18 E2 proteins. (psjhealth.org)