RatsGenesPhosphorylationMiceMRNA and proteinNeuronsKinasePromoterInduceCellsVitroTranscription FactorsMeSHImmunocytochemistryStimulationCFosActivationKinasesHumansImmunohistochemistryStriatalImmunocytochemicalPathwaysTumorSerumBehavioralCell-cycleHeterodimerPathwayComplexesSubfamilyExpression in the ratNucleusComplexInductionAntibodiesRegulationCellularApoptosisChromosomeTranscriptionalOverexpressionSignificantlyMorphineLeucine
Rats8
- Artificial rearing causes changes in maternal behavior and c-fos expression in juvenile female rats. (mcmaster.ca)
- We found a significant increase, in both gene and protein expression, of the neurotrophin brain-derived neurotrophic factor (BDNF), in the prefrontal cortex (PFC) of adult TPH2-deficient (TPH2 −/− ) male and female rats in comparison to wild type (TPH2 +/+ ) counterparts. (frontiersin.org)
- Experiment 1 assessed the expression of the immediate-early gene c-fos in rats that discriminated novel from familiar objects in the dark (Group Novel). (elsevierpure.com)
- In protein expression analysis, protein kinase C (PKC), growth-associated protein 43 (GAP43) and phosphorylated at serine 41 GAP43 (p-GAP43) were significantly increased after voluntary exercise compared with those in rats without exercise. (fujita-hu.ac.jp)
- Acute administration of naloxone and chronic administration of morphine did not change the expression of Fos protein and NADPH-d positive neurons, and there was no expression of Fos/NADPH-d double-labeled neurons in the spinal cord of rats. (unboundmedicine.com)
- Light has been shown to induce Fos-like immunoreactivity (Fos-LI) in the SCN of rats and hamsters. (ox.ac.uk)
- Initial immunohistochemical measurements of the marker of neural activation, Fos, confirmed that in halothane-anesthetized rats fenfluramine (10 mg/kg i.v.) evoked cellular responses in cortical regions which were attenuated by pre-treatment with the 5-HT synthesis inhibitor p-chlorophenylalanine (300 mg/kg i.p. once daily for 2 days). (ox.ac.uk)
- Phosphorylation of cAMP response element-binding protein in the extended amygdala of male rats is induced by novel environment and attenuated by estrous female-bedding. (nel.edu)
Genes6
- c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. (wikipedia.org)
- Although the pattern of corticosterone release was similar between the genotypes, neuronal activation in response to stress, quantified by the expression of the immediate early genes activity regulated cytoskeleton associated protein ( Arc ) and Fos Proto-Oncogene ( cFos ), was blunted in both sexes of animals lacking brain serotonin. (frontiersin.org)
- Local calcium entry through CRAC channels activates expression of c-fos- and nuclear factor of activated T cells (NFAT)-dependent genes. (ox.ac.uk)
- Although c-fos and NFAT often interact to activate gene expression synergistically, they can be activated independently of one another to regulate distinct genes. (ox.ac.uk)
- Although placental protein 14, leukemia inhibitory factor and 17-beta hydroxysteroid dehydrogenase displayed highly upregulated levels in PcDNA (P-activated genes), there was little or no up-regulation in IcDNA. (umassmed.edu)
- Regulators of the cell cycle and transcription, such as retinoblastoma, c-fos, and c-jun genes, were also greatly underexpressed in IcDNA compared with PcDNA. (umassmed.edu)
Phosphorylation14
- In the present study, we examined the role of protein tyrosine phosphorylation and the mitogen-activated protein kinases, ERK1/2,in mediating BK-induced AP-1 and DNA replication in cultured rat mesangial cells. (nih.gov)
- BK (10(-9) to 10(-7) M) stimulated a rapid increase in tyrosine phosphorylation of multiple proteins with an estimated molecular mass of 120-130, 90-95, and 44-42 kDa. (nih.gov)
- 0.05) in phosphorylation of Elk-1, a transcription factor required for growth factor-induced c-fos transcription. (nih.gov)
- In accord with the stimulation of Elk-1 phosphorylation, BK induced c-fos gene expression and the production of Fos/AP-1 complexes. (nih.gov)
- AP-1 pathway and that BK mitogenic signaling is critically dependent on protein tyrosine phosphorylation. (nih.gov)
- Phosphorylation of the C terminus of c-Fos has been implicated in serum response element-mediated repression of c-fos transcription after its induction by serum growth factors. (nih.gov)
- We now provide evidence that two growth-regulated, nucleus- and cytoplasm-localized protein kinases, 90-kDa ribosomal S6 kinase (RSK) and mitogen-activated protein kinase (MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. (nih.gov)
- The major phosphopeptides derived from biosynthetically labeled c-Fos correspond to phosphopeptides generated after phosphorylation of c-Fos in vitro with both RSK and MAP kinase. (nih.gov)
- This study suggests a role for nuclear RSK and MAP kinase in modulating newly synthesized c-Fos phosphorylation and downstream signaling. (nih.gov)
- Its activity is also regulated by posttranslational modification caused by phosphorylation by different kinases, like MAPK, CDC2, PKA or PKC which influence protein stability, DNA-binding activity and the trans-activating potential of the transcription factors. (wikipedia.org)
- The expression and localization of these proteins may be related to the underlying mechanisms of exercise-induced paralysis recovery, that is, neuronal plasticity and remodeling of cortical connections through the phosphorylation of GAP43 by interaction with PKC. (fujita-hu.ac.jp)
- Mdm2 Phosphorylation Regulates Its Stability and Has Contrasting Effects on Oncogene and Radiation-Induced Tumorigenesis. (umassmed.edu)
- Phosphorylation of tyrosine 14 on caveolin-1 regulates CRAC channel-evoked c-fos activation without impacting the NFAT pathway or Orai1 activity. (ox.ac.uk)
- Therefore, the aim of the present study was to measure D1 and D2 dopamine receptor expression and related signaling, such as CREB and ERK protein levels and phosphorylation, in the hippocampus and other brain regions of mice with post-natal CRF over-expression (CRF-OE mice). (nel.edu)
Mice5
- The transgenic mice thus generated are viable, demonstrating that there are c-fos dependent and independent pathways of cell proliferation, but display a range of tissue-specific developmental defects, including osteoporosis, delayed gametogenesis, lymphopenia and behavioral abnormalities. (wikipedia.org)
- It was found that overexpression of c-fos from class I MHC promoter in transgenic mice leads to the formation of osteosarcomas due to increased proliferation of osteoblasts whereas ectopic expression of the other Jun and Fos proteins does not induce any malignant tumors. (wikipedia.org)
- Activation of the c-Fos transgene in mice results in overexpression of cyclin D1, A and E in osteoblasts and chondrocytes, both in vitro and in vivo, which might contribute to the uncontrolled growth leading to tumor. (wikipedia.org)
- Corticotropin-releasing factor (CRF) over-expression down-regulates hippocampal dopamine receptor protein expression and CREB activation in mice. (nel.edu)
- We found a region-specific down-regulation of both D1 and D2 protein expression, without altered CRF receptor protein expression, in the hippocampus in CRF-OE mice. (nel.edu)
MRNA and protein2
- The c-fos mRNA and protein is generally among the first to be expressed and hence referred to as an immediate early gene. (wikipedia.org)
- Challenge of rGMCs with 1 or 10 μM HgCl 2 or Pb 2 acetate increased HSPA5 mRNA and protein levels. (bioone.org)
Neurons3
- Noxious stimulation of the cornea induced c-Fos in neurons sending projections to parabrachial nuclei, but not thalamic nuclei. (elsevierpure.com)
- Morphine withdrawal increased the expression of Fos protein, NADPH-d positive, and Fos/NADPH-d double-labeled neurons, and they were observed in all the laminae of the rat spinal cord. (unboundmedicine.com)
- Fenfluramine-induced Fos was demonstrated in numerous glutamatergic pyramidal neurons (Fos/excitatory amino acid carrier 1 (EAAC1) co-labeled), but also a small number of GABA interneurons (Fos/glutamic acid decarboxylase (GAD)(67) colabeled). (ox.ac.uk)
Kinase6
- In contrast, protein kinase C inhibition or depletion had no effect on BK-induced c-fos mRNA, AP-1-DNA binding activity, or DNA synthesis. (nih.gov)
- The exact mechanism by which c-Fos contributes to apoptosis is not clearly understood, but observations in human hepatocellular carcinoma cells indicate that c-Fos is a mediator of c-myc-induced cell death and might induce apoptosis through the p38 MAP kinase pathway. (wikipedia.org)
- cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. (cshlpress.com)
- Cyclin-dependent kinase 2 (CDK2) is a key mediator for EGF-induced cell transformation mediated through the ELK4/c-Fos signaling pathway. (nih.gov)
- In addition to the scavengers of reactive oxygen species and calcium, inhibitors specific for transcription (actinomycin D), protein kinase C (RO-31-8220), and MAP kinase (PD 98059) also blocked the cadmium -induced overexpression of the proto-oncogenes in the tumor cells. (cdc.gov)
- Further, the cadmium -induced overexpression of the proto-oncogenes is dependent on transcriptional activation as well as on pathways involving protein kinase C and MAP kinase. (cdc.gov)
Promoter5
- This double action could be enabled by differential protein composition of tumour cells and their environment, for example, dimerisation partners, co-activators and promoter architecture. (wikipedia.org)
- Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. (nih.gov)
- Thus, OSM (but not IL-6) stimulates c-Fos, which participates in maximal activation of TIMP-1 transcription, likely in cooperation with other factors such as SP1 or as yet unidentified mechanisms involving the +1 to +47 region of the promoter. (uea.ac.uk)
- Component of the SMAD3/SMAD4/JUN/FOS complex required for synergistic TGF-beta-mediated transcription at the AP1 promoter site. (icr.ac.uk)
- Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. (bvsalud.org)
Induce3
- This experiment examined whether exposure to an inter-temporal choice would induce neuronal activation in these areas, as indicated by enhanced expression of the Fos protein. (elsevierpure.com)
- Brief pulses of light administered to animals otherwise in constant darkness were found to induce Fos-LI. (ox.ac.uk)
- Phorbol 13-myristate 12-acetate could also induce c-Fos but could not regulate the TIMP-1 reporter gene constructs. (uea.ac.uk)
Cells11
- Cells differentiating into neuroectoderm undergo apoptosis in the absence of functional retinoblastoma family proteins. (mcmaster.ca)
- Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
- All taken together, these observations underline the complexity of the mechanisms responsible for the selective destruction of proteins within cells. (cnrs.fr)
- Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
- Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. (cshlpress.com)
- In immune cells, calcium entry occurs through store-operated Ca(2+) release-activated Ca(2+) (CRAC) channels, comprised of STIM1 and Orai1 proteins. (ox.ac.uk)
- We examined whether female pheromone, which would be contained in female-soiled bedding, affected the expression of phosphorylated cAMP response element-binding protein-like (pCREB) immunoreactive cells in the extended amygdala. (nel.edu)
- This confirmed that the overexpression of the proto-oncogenes in the tumor cells required elevated intracellular levels of reactive oxygen species and calcium. (cdc.gov)
- Exposure of the nontransformed BALB/c-3T3 cells to 20 microM cadmium chloride for 1 h caused elevated intracellular levels of superoxide anion, hydrogen peroxide, and calcium, with corresponding increases in the expression levels of c-fos, c-jun, and c-myc. (cdc.gov)
- As in the case of the tumor cells, treating the nontransformed cells with the various modulators prior to their exposure to cadmium chloride resulted in inhibition in the expression of the proto-oncogenes. (cdc.gov)
- 2. Dai, T. et al: Stress-activated protein kinases bind directly to the delta domain of c-Jun in resting cells: implications for repression of c-Jun function. (signalchem.com)
Vitro3
- In vitro studies have shown that Jun-Fos heterodimers are more stable and have stronger DNA-binding activity than Jun-Jun homodimers. (wikipedia.org)
- The c-myc protein is localized in the nucleus and can physically associate with RB protein in vitro, hence c-myc may functionally antagonize RB function. (wdv.com)
- Intended Use Human c Fos ELISA Kit allows for the in vitro quantitative determination of c Fos , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids. (biobool.com)
Transcription Factors3
- It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. (wikipedia.org)
- The c-Fos and c-Jun oncoproteins and the p53 tumor suppressor protein are short-lived transcription factors. (cnrs.fr)
- RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)
MeSH1
- Proto-Oncogene Proteins c-mdm2" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
Immunocytochemistry1
- Fos immunocytochemistry, NADPH-d histochemistry, Fos/NADPH-d double-labeling, intrathecal injection, antisense oligonucleotides (AS-ONs) techniques, and RT-PCR were used. (unboundmedicine.com)
Stimulation1
- We used immunocytochemical detection of c-Fos protein as an index of neuronal activation after noxious ocular stimulation. (elsevierpure.com)
CFos1
- The peptide is homologous with the corresponding sequence derived from cFos protein human, rat, mouse, hamster and cat. (biosensis.com)
Activation6
- Moreover, c-Fos, c-Jun and p53 turnovers are regulated upon activation of intracellular signalling cascades. (cnrs.fr)
- While there may also be a relative increase in c-fos activation in the extended-hippocampal system to object recognition in the dark, there was no evidence that this recognition memory problem required an intact hippocampus. (elsevierpure.com)
- On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. (icr.ac.uk)
- Cadmium -induced cell transformation and tumorigenesis are associated with transcriptional activation of c-fos, c-jun and c-myc proto-oncogenes: role of cellular calcium and reactive oxygen species. (cdc.gov)
- c-Jun is a proto-oncogene that forms a complex with c-Fos which regulates transcription from promoters containing AP-1 activation elements (1). (signalchem.com)
- Environmental injury has been associated with endoplasmic reticulum (ER) stress, a response characterized by activation of the unfolded protein response, proteasomal degradation of proteins, and induction of HSPA5, also known as GRP78 or BiP. (bioone.org)
Kinases1
- The protein encoded by this gene is phosphorylated by the kinases, MAPK1 and MAPK8. (nih.gov)
Humans1
- It is encoded in humans by the FOS gene. (wikipedia.org)
Immunohistochemistry1
- Specificity This antiserum shows a high level of specificity for c-FOS confirmed by immunohistochemistry. (biosensis.com)
Striatal1
- This mixture also reduced amphetamine-induced fos expression in striatal areas. (ox.ac.uk)
Immunocytochemical1
- Measurements of regional brain tyrosine levels, catecholamine and indoleamine synthesis (L-DOPA and 5-HTP accumulation, respectively) were used to identify an effective paradigm to test in neurochemical, behavioral and fos immunocytochemical models. (ox.ac.uk)
Pathways1
- Are there multiple proteolytic pathways contributing to c-Fos, c-Jun and p53 protein degradation in vivo? (cnrs.fr)
Tumor3
- Several studies have raised the idea that c-Fos may also have tumor-suppressor activity, that it might be able to promote as well as suppress tumorigenesis. (wikipedia.org)
- nc886's tumor suppressive role is corroborated by the induction of well-known oncogenes such as FOS, NF-κB, and MYC upon its knockdown. (oncotarget.com)
- Its ability to ubiquitinate p53 is regulated by TUMOR SUPPRESSOR PROTEIN P14ARF. (umassmed.edu)
Serum1
- Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element. (nih.gov)
Behavioral1
- Immunohistochemical localization of c-Fos protein after behavioral test occurred in the area surrounding the infarction core in the exercise group. (fujita-hu.ac.jp)
Cell-cycle1
- Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle. (wdv.com)
Heterodimer1
Pathway1
- More generally, our findings reveal that modular regulation by a scaffolding protein provides a simple, yet effective, mechanism to tunnel a local signal down a specific pathway. (ox.ac.uk)
Complexes1
- 1. Halazonetis, T D. et al: c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinitie. (signalchem.com)
Subfamily1
- Fos subfamily. (biosensis.com)
Expression in the rat1
- Intrathecal injection of nNOS antisense oligonucleotides (nNOS-AS) inhibited the increase of Fos protein and NMDA(1A)R mRNA expression in the rat spinal cord during morphine withdrawal and decreased the scores of morphine withdrawal symptoms. (unboundmedicine.com)
Nucleus2
- Outside the temporal region, Group Novel showed relatively increased c-fos activity in the anterior medial thalamic nucleus and the anterior cingulate cortex. (elsevierpure.com)
- Photic regulation of Fos-like immunoreactivity in the suprachiasmatic nucleus of the mouse. (ox.ac.uk)
Complex3
- Here, we show that the lipid raft scaffolding protein caveolin-1 interacts with the STIM1-Orai1 complex to increase channel activity. (ox.ac.uk)
- Fos and Jun nuclear factors bound constitutively to this site as identified by supershift analysis in electrophoretic mobility shift assays, and oncostatin M (but not IL-6) induced an additional "complex 2" that contained c-Fos and JunD. (uea.ac.uk)
- OSM stimulated a rapid and transient increase in c-Fos mRNA and nuclear protein that coincided with complex 2 formation. (uea.ac.uk)
Induction1
- Phases at which light causes phase shifts of the circadian system were permissive for Fos-LI induction (CT 16 and 24), while phases in which light does not cause phase shifts were not permissive (CT 6 and 9). (ox.ac.uk)
Antibodies1
- The importance of c-fos in biological context has been determined by eliminating endogenous function by using anti-sense mRNA, anti-c-fos antibodies, a ribozyme that cleaves c-fos mRNA or a dominant negative mutant of c-fos. (wikipedia.org)
Regulation2
- Light regulation of Fos-LI was dependent upon the phase of the circadian cycle in which the light pulse was administered. (ox.ac.uk)
- These results have implications regarding the possible functional roles of Fos in the circadian system and add to our understanding of light regulation of circadian physiology in the mouse. (ox.ac.uk)
Cellular3
- Product Description google Sheep anti-Cellular oncogene fos (c-Fos) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen. (biosensis.com)
- Based on these data, we conclude that the cadmium -induced overexpression of cellular proto-oncogenes is mediated by the elevation of intracellular levels of superoxide anion, hydrogen peroxide, and calcium. (cdc.gov)
- Molecular chaperones are known to play a role in stabilizing protein structure and function during events of cellular stress. (bioone.org)
Apoptosis1
- Fas ligand (FASLG or FasL) and the tumour necrosis factor-related apoptosis-inducing ligand (TNFSF10 or TRAIL) might reflect an additional apoptotic mechanism induced by c-Fos, as observed in a human T-cell leukaemia cell line. (wikipedia.org)
Chromosome1
- Mayr D, Hirschmann A, Marlow S, Horvath C, Diebold J. Analysis of selected oncogenes (AKT1, FOS, BCL2L2, TGFbeta) on chromosome 14 in granulosa cell tumors (GCTs): a comprehensive study on 30 GCTs combining comparative genomic hybridization (CGH) and fluorescence-in situ-hybridization (FISH). (cancercentrum.se)
Transcriptional1
- Proteína transcriptional, que actúa en trans, de los elementos promotores que se encuentran en las repeticiones largas del extremo (LTR) del HTLV-I y HTLV-II. (bvsalud.org)
Overexpression2
- In osteosarcoma and endometrial carcinoma, c-Fos overexpression was associated with high-grade lesions and poor prognosis. (wikipedia.org)
- The proto-oncogenes c-fos and c-jun were overexpressed in 100% (10 out of 10) of the cell lines, while a statistically significant overexpression of c-myc was observed in 40% (4 out of 10) of the cell lines. (cdc.gov)
Significantly2
- Also, in a comparison between precancerous lesion of the cervix uteri and invasive cervical cancer, c-Fos expression was significantly lower in precancerous lesions. (wikipedia.org)
- Abrogated PDGF signalling in CD248(-/-) HSC was confirmed by significantly reduced c-fos expression in CD248(-/-) HSC compared with wild-type HSC. (ox.ac.uk)
Morphine1
- TY - JOUR T1 - NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal. (unboundmedicine.com)
Leucine1
- c-Fos is a 380 amino acid protein with a basic leucine zipper region for dimerisation and DNA-binding and a transactivation domain at C-terminus, and, like Jun proteins, it can form homodimers. (wikipedia.org)