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  • Mice
  • Urethane-induced lung tumors in mice frequently harbor mutations in the Kras oncogene, and in this study, we use this model to address the regulation of p27 during tumorigenesis. (fhcrc.org)
  • cells
  • Resistance to chemotherapeutic agents and promotion of transforming activity mediated by embryonic stem cell-expressed Ras (ERas) signal in neuroblastoma cells. (biomedsearch.com)
  • In the present study, we investigated the expression and function of embryonic stem cell-expressed Ras (ERas), a novel Ras family protein previously reported as the specific expression gene in embryonic stem cells (ES cells), in neuroblastoma cell lines. (biomedsearch.com)
  • MDCK cells undergoing Ras-induced EMT remodel the extracellular matrix (ECM) via diminished expression of basement membrane constituents (collagen type IV and laminin 5), up-regulation of extracellular proteases (MMP-1, kallikreins -6 and -7), and increased production and secretion of ECM constituents (SPARC, collagen type I, fibulins -1 and -3, biglycan, and decorin). (edu.au)
  • Lysates of such desensitized cells were quantitatively immunoprecipitated with an antiserum recognizing both Sos1 and Sos2 proteins or a specific anti-Sos2 antiserum. (umassmed.edu)
  • These data are consistent with the hypothesis that the dissociation of Grb2 from Sos proteins caused by insulin in 3T3-L1 cells mediates p21ras deactivation and desensitization. (umassmed.edu)
  • Following treatment with bioactive agents, the expression of miR-let7-a was specifically induced in cancer cells but not in normal cells and it was associated with K-ras inhibition. (ox.ac.uk)
  • A. Okura, H. Arakawa, H. Oka, T. Yoshinari, and Y. Monden, Effect of genistein on topoisomerase activity and on the growth of (val 12) Ha-ras-transformed NIH 3T3 cells, Biochem Biophys Res Commun 157: 183-189(1988). (springer.com)
  • Gene
  • What does this gene/protein do? (cancerindex.org)
  • The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. (cancerindex.org)
  • pharmacological
  • therefore, it is reasonable to speculate that a pharmacological approach that curtails Ras activity may represent a sensible approach to inhibit melanoma growth. (curehunter.com)
  • immunohistochemical
  • A-C ) β-Galactosidase staining at pH 6, ( D-F ) p53 immunohistochemical staining, ( G-I ) p21 immunohistochemical staining, and ( J-L ) MCM2 immunohistochemical staining, in sections of frozen (β-galactosidase) or formalin-fixed paraffin-embedded pancreatic tissue. (nih.gov)
  • disassembly
  • Taken together, these data indicate that insulin, EGF, and PDGF all cause disassembly of Sos proteins from signaling complexes during p21ras desensitization, but at least two mechanisms are involved. (umassmed.edu)
  • PDGF action triggers both mechanisms of Grb2 disassembly, which probably operate in concert with GAP to attenuate p21ras signaling. (umassmed.edu)
  • Disassembly of Son-of-sevenless proteins from Grb2 during p21ras desen" by Andrew D. Cherniack, Jes K. Klarlund et al. (umassmed.edu)
  • The disassembly of Grb2 from Sos proteins slightly precedes the time course of p21ras deactivation in response to insulin. (umassmed.edu)
  • inhibition
  • Sulforaphane, quercetin and catechins complement each other in elimination of advanced pancreatic cancer by miR-let-7 induction and K-ras inhibition. (ox.ac.uk)
  • These data demonstrate that sulforaphane, quercetin and GTC complement each other in inhibition of PDA progression by induction of miR-let7-a and inhibition of K-ras. (ox.ac.uk)
  • suppresses
  • This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). (cancerindex.org)
  • either the PIP2 binder HS1 or the Rac blocker SCH51344 restores the ability of EMS1 to bind the complex and suppresses the oncogenicity of Ras. (edu.au)
  • Nucleotide
  • Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. (umassmed.edu)
  • SEQUENCE
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Among AS ODNs tested, 17mer AS ODN centered around codon 12 of v-Ki- rasinhibited the v-Ki-Ras expression and v-Ki-Ras-induced anchorage-independent growth, while its sense sequence did not affect it. (naver.com)
  • insulin
  • Furthermore, elevated amounts of Grb2/Shc complexes persisted throughout p21ras desensitization by insulin. (umassmed.edu)
  • However, immunoblotting of anti-Son-of-sevenless (Sos) 1 and 2 immunoprecipitates with anti-Grb2 antisera revealed that p21ras desensitization in response to insulin and PDGF, but not EGF, is associated with a marked decrease in cellular complexes containing Sos and Grb2 proteins. (umassmed.edu)
  • Immunoblot analysis of these precipitates revealed that insulin causes a marked hyperphosphorylation of Sos1 and a 50% decrease in Grb2 associated with Sos proteins under these conditions. (umassmed.edu)
  • Similarly, anti-Grb2 immunoprecipitates of such lysates revealed the presence of decreased Sos1 protein due to insulin action. (umassmed.edu)