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Gene AmplificationIn Situ Hybridization, FluorescenceNucleic Acid Amplification TechniquesGenes, erbB-2Receptor, erbB-2Gene DosagePolymerase Chain ReactionPhosphonoacetic AcidDihydroorotaseDrug ResistanceTetrahydrofolate DehydrogenaseBase SequenceGenes, mycMolecular Sequence DataBlotting, SouthernImmunohistochemistryBreast NeoplasmsDNA, NeoplasmGene Expression Regulation, NeoplasticReceptor, Epidermal Growth FactorChromosomes, Human, Pair 17Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)Nucleic Acid HybridizationTissue Array AnalysisMutationOncogenesMethotrexateDNA PrimersParaffin EmbeddingChromogenic CompoundsTumor Markers, BiologicalSensitivity and SpecificityAspartate CarbamoyltransferaseGenes, erbB-1Chromosome AberrationsDNANeuroblastomaRNA, MessengerAneuploidyTumor Cells, CulturedPrognosisSequence Analysis, DNAAmaranthusChorionDNA, BacterialIn Situ HybridizationFibroblast Growth Factor 3Proto-OncogenesCell LineProto-Oncogene ProteinsCell Line, TumorReverse Transcriptase Polymerase Chain ReactionAdenocarcinomaKaryotypingRNA, NeoplasmProto-Oncogene Proteins c-mdm2DNA ProbesCricetinaeOncogene ProteinsGene RearrangementCricetulusLung NeoplasmsChromosome MappingCyclin D1Cloning, MolecularAspartic AcidComparative Genomic HybridizationProto-Oncogene Proteins c-mycDNA ReplicationDNA Mutational AnalysisGene ExpressionNeoplasm StagingCarcinoma, Ductal, BreastNeoplasm ProteinsDrug Resistance, NeoplasmChromosomes, Human, Pair 11DNA Topoisomerases, Type IIOligonucleotide Array Sequence AnalysisEsophageal NeoplasmsAntineoplastic AgentsCarcinoma, Squamous CellPlasmidsChromosomes, Human, Pair 7Gene Expression ProfilingNuclear ProteinsRepetitive Sequences, Nucleic AcidDNA, ProtozoanImmunoenzyme TechniquesGenesReproducibility of ResultsNeoplasm InvasivenessAntibodies, Monoclonal, HumanizedChromosomes, Human, Pair 20Genes, p53Proto-Oncogene Proteins c-metDNA Copy Number VariationsCarcinomaGenes, rasDNA-Binding ProteinsElectrophoresis, Agar Gel
Whole genome amplifMultiple displacement amplificationGenomeParaffin-embeddedPolymeraseGenomicMethodsAnalysisVariety
Whole genome amplif11
  • 2. Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues. (nih.gov)
  • 9. A whole-genome amplification protocol for a wide variety of DNAs, including those from formalin-fixed and paraffin-embedded tissue. (nih.gov)
  • Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. (justia.com)
  • In certain embodiments, whole genome amplification (WGA) is carried out such that a reaction plateau is not reached. (justia.com)
  • Our Whole Genome Amplification (WGA) product line provides a robust and accurate method of amplifying nanogram quantities of starting material into microgram yields with minimal allele drop out. (sigmaaldrich.com)
  • We continue to expand our WGA product line to offer you a range of solutions for whole genome amplification from a variety of starting material, including buccal swabs, fresh tissue samples, frozen tissue samples, blood cards, peripheral blood and formalin-fixed, paraffin-embedded (FFPE) tissues. (sigmaaldrich.com)
  • The GenomePlex ® Whole Genome Amplification (WGA) kits have been developed for use as a high-throughput system for the rapid and highly representative, amplification of genomic DNA from trace amounts of starting material. (sigmaaldrich.com)
  • The GenomePlex ® Single Cell Whole Genome Amplification Kit (WGA4) is designed to amplify the genome of a single cell resulting in a million-fold amplification yielding microgram quantities of genomic DNA. (sigmaaldrich.com)
  • Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. (sigmaaldrich.com)
  • The Single Cell WGA kit includes all the reagents necessary for cell lysis and subsequent whole genome amplification. (sigmaaldrich.com)
  • The SeqPlex-I DNA and RNA Amplification Kits for whole genome amplification (WGA) and whole transcriptome amplification (WTA) are designed to facilitate Illumina ® next-generation sequencing (NGS) from extremely small quantities or degraded/highly fragmented DNA and RNA. (sigmaaldrich.com)
Multiple displacement amplification2
  • 19. Comparison of yield and genotyping performance of multiple displacement amplification and OmniPlex whole genome amplified DNA generated from multiple DNA sources. (nih.gov)
  • Suitable WGA techniques include primer extension PCR (PEP), degenerated oligonucleotide primed PCR, ligation-mediated PCR (LMP), the T7-based linear amplification of DNA (TLAD), and multiple displacement amplification (MDA). (justia.com)
Genome1
Paraffin-embedded1
  • 17. Feasibility of archival non-buffered formalin-fixed and paraffin-embedded tissues for PCR amplification: an analysis of resected gastric carcinoma. (nih.gov)
Polymerase1
  • However, the GenomePlex ® technology provides amplification efficiency with WGA DNA Polymerase. (sigmaaldrich.com)
Genomic1
  • The GenomePlex ® WGA kits utilize a proprietary amplification technology based upon random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable OmniPlex ® Library molecules flanked by universal priming sites. (sigmaaldrich.com)
Methods1
  • Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids. (justia.com)
Analysis1
  • 3. Multiple strand displacement amplification of DNA isolated from human archival plasma/serum: identification of cytokine polymorphism by pyrosequencing analysis. (nih.gov)
Variety1
  • The Single Cell WGA kit has been validated with numerous downstream applications and is suitable for amplification from a variety of sources including plant, polycarbonate fixed cells, lymphocytes, renal cancer cells, epithelial cells, leukemia cells, and fibroblast amniotic cells. (sigmaaldrich.com)