Oligonucleotide sequence branched dna signal amplification assay. Medical search. Frequent questions

These 'guide' crRNAs are incorporated into a ribonucleoprotein interference complex that can recognize and cleave its complementary target sequence, thus destroying the invading nucleic acid. (elifesciences.org)

A variety of methodologies for labeling DNA are used to generate end-labeled or continuously labeled probes. (enzolifesciences.com)
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes. (uams.edu)

The branched-DNA (bDNA) assay provides a reliable method for the quantification of HIV-1 RNA in human plasma and is considered one of the most reproducible assays ready for use in clinical trials. (ox.ac.uk)
A series of oligonucleotide probe design and solution changes have been developed to enhance the sensitivity of the bDNA assay while maintaining its performance characteristics. (ox.ac.uk)
Among the changes incorporated into the enhanced-sensitivity bDNA (ES bDNA) assay to reduce the background level and enhance the signal are the use of shorter overhang sequences of target probes for capture, the cruciform design of target probes for amplification, and the addition of preamplifier molecules. (ox.ac.uk)
The ES bDNA assay is at least 20-fold more sensitive than the first-generation bDNA assay, yet it maintains a high level of accuracy, linearity, and reproducibility. (ox.ac.uk)
Further, quantification values obtained with the ES bDNA assay and the first-generation bDNA assay are highly correlated, thus allowing for meaningful comparisons of HIV-1 RNA levels in specimens tested with either assay. (ox.ac.uk)
The ES bDNA assay may be useful in determining the prognostic value of HIV-1 RNA levels of below 10,000 copies per ml and in assessing the clinical benefit of antiretroviral therapy-induced decreases in plasma HIV-1 RNA sustained at levels of below 10,000 copies per ml. (ox.ac.uk)
The assay combines paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect up to three RNA transcripts at the single-cell level using a standard flow cytometer. (technion.ac.il)

DNA microarrays based on the selected probes were used to type 92 clinical enterobacterial isolates. (biomedcentral.com)
This study aimed at the identification of prognostic gene expression markers in early primary colorectal carcinomas without metastasis at the time point of surgery by analyzing genome-wide gene expression profiles using oligonucleotide microarrays. (biomedcentral.com)
Gene expression profiling was performed using Whole Human Genome 4x44K Oligonucleotide Microarrays. (biomedcentral.com)

Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
The MGB moiety at the 3' end increases the melting temperature (Tm) and stabilises binding, allowing a shorter probe with improved sequence specificity. (biosearchtech.com)
The Panel does not recommend use of the currently approved HIV p24 antigen assay for infant diagnosis in the United States because the sensitivity and specificity of the assay in the first months of life is less than that of other HIV virologic tests. (medscape.com)
A method for determining the sequence specificity of DNA-binding proteins. (lookformedical.com)

In a whole-genome sequencing in 17 Esophageal Squamous Cell Carcinoma cases and whole-exome sequencing in 71 cases, Song (2014), reported ASH1L alterations. (cancerindex.org)
Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing. (cdc.gov)
SWGA is a robust method that allows direct whole-genome sequencing (WGS) of specimens containing very low numbers of T. pallidum, which has been challenging until now. (cdc.gov)

Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens) in a biological sample obtained from a subject. (justia.com)
The disclosure generally relates to methods for detecting the presence of a target nucleic acid sequence. (justia.com)

this included the TNF‑α pathway, the NF‑κB pathway, the MAPK signaling pathway, the Notch signaling pathway and Axon guidance. (spandidos-publications.com)
Collectively, the present study provides insights into AEG‑1‑dependent gene regulation and signaling pathway transduction in neurons. (spandidos-publications.com)

Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. (cdc.gov)
The discriminative power of the probe set was further tested in silico on 15 additional complete enterobacterial genome sequences. (biomedcentral.com)
Sequence analysis of the orangutan genome revealed that recent proliferative activity of Alu elements has been uncharacteristically quiescent in the Pongo (orangutan) lineage, compared with all previously studied primate genomes. (biomedcentral.com)
Due to the staggered DNA cuts of the genome by the L1-derived endonuclease during TPRT, Alu insertions are flanked by short sequences of duplicated host DNA called target site duplications (TSDs), which can be used to identify the insertion event. (biomedcentral.com)

The annealed primers ultimately become part of the probe itself, because the Klenow fragment of DNA polymerase I extends the primers in the 3′ direction and, in so doing, incorporates the label. (enzolifesciences.com)
Next, the enzyme DNA polymerase I removes the native nucleotides from the probe molecules in the 5′→3′ direction (exonuclease activity) while replacing them with labeled dNTP precursors by virtue of its 5′→3′ polymerase activity. (enzolifesciences.com)
The CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay is a real-time reverse-transcription polymerase chain reaction (rRT-PCR) laboratory test that can simultaneously detect and differentiate between influenza A, influenza B, and SARS-CoV-2 in upper or lower respiratory specimens. (cdc.gov)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)

In theory, any nucleic acid can be used as a probe provided it can be labeled to permit detection and quantitation of the hybrid molecules formed between the probe and sequence to be identified. (enzolifesciences.com)
This novel assay employs a proprietary fluorescent in situ hybridization (FISH) technique enabling simultaneous detection of up to three RNA transcripts in a single cell using a standard flow cytometer. (technion.ac.il)
Assays were run on two different Biosearch Technologies low-volume, high-throughput automation platforms, allowing for liquid handling, thermal cycling and fluorescence detection/analysis. (biosearchtech.com)
We developed a DNA microarray for strain typing and detection of major antimicrobial resistance genes of clinically relevant enterobacteria. (biomedcentral.com)
OBJECTIVE: To evaluate the field performance of a multiplex PCR (M-PCR) assay for detection of herpes simplex virus (HSV)-1 and HSV-2, Treponema pallidum (T. pallidum) and Haemophilus ducreyi (H. ducreyi) in genital ulcer disease (GUD) specimens. (cdc.gov)
In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent detection of the amplification products. (justia.com)

Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
The X-ray structure of the casposase bound to DNA representing the product of integration reveals a tetramer with target DNA bound snugly between two dimers in which single-stranded casposon end binding resembles that of spacer 3'-overhangs. (elifesciences.org)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)

A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
It is also called nucleotide sequence. (lookformedical.com)

Downstream next-generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum (T. pallidum) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. (cdc.gov)

In homogeneous immunoassays, the antibody reacts with the analyte in solution, which allows much faster assay times. (trea.com)
By adding additional unlabeled analyte, via applying a sample, the steady state shifts and more fluorescence signal can be quenched by the fluorophore specific antibody. (trea.com)
When the peptide is bound by the antibody, the dark quencher and the fluorophore are in spatial proximity, resulting into a reduced fluorescence signal. (trea.com)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
Variant analysis using sequencing data derived from SWGA-enriched specimens showed that all 5 clinical strains had the A2058G mutation associated with azithromycin resistance. (cdc.gov)
The significance of our research is the development of an SWGA DNA enrichment method that expands the range of clinical specimens that can be directly sequenced to include samples with low numbers of T. pallidum. (cdc.gov)
Because the steady state of this reaction can be reached already after 90 seconds, this assay is highly attractive for clinical point of care testing (POCT). (trea.com)

An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma. (ox.ac.uk)

80%) and biological pathways (such as adipocytokine, Notch, Hedgehog and NOD-like receptor signaling) were not identified by previous gene array studies. (mdpi.com)
The trk receptors, a family of receptor tyrosine kinases including trkA, trkB, and trkC, serve as the principal signal-transducing receptors for the neurotrophins ( Barbacid, 1994 ). (jneurosci.org)
We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
The majority of CRPCs continue to express the androgen receptor (AR) and are dependent on AR signaling for malignant progression [ 3 - 5 ]. (oncotarget.com)

Most current methods for colorimetric lateral flow readout of Loop-mediated isothermal amplification (LAMP) amplicons rely on non-specific labeling methods such as incorporation of labeled primers or nucleotides. (justia.com)
Iron levels were detected by immunofluorescence staining and colorimetric assays. (stanfordchildrens.org)

METHODS: GUD M-PCR was performed on 186 remnant specimens, previously collected for HSV testing, by four public health laboratories (PHLs) and the Laboratory Reference and Research Branch (LRRB) at the Centers for Disease Control and Prevention. (cdc.gov)

However, some scientists think that before life adopted DNA and proteins, it relied primarily on RNA. (elifesciences.org)
All life on Earth today uses a triplet RNA code to make proteins from DNA, and these experiments showed how RNA triplets might have helped RNA sustain early life forms. (elifesciences.org)
Products of the former group function directly in protecting cells against damage derived from stresses and sustaining cell viability, and the latter is composed of regulatory proteins, such as transcription factors (TFs), protein kinases and protein phosphatases, that are responsible for transducing stress signaling and regulating expression of stress-responsive genes [ 5 ]. (biomedcentral.com)
Proteins which bind to DNA. (lookformedical.com)

Altogether, by identifying a pro-angiogenic VEGF/miR-101/EZH2 axis in endothelial cells we provide evidence for a functional link between growth factor-mediated signaling, post-transcriptional silencing, and histone-methylation in the angiogenesis process. (plos.org)

BCL2 antibodies targeted at different epitopes detect varying levels of protein expression and correlate with frequent gene amplification in diffuse large B-cell lymphoma. (uams.edu)

Mutation of FUL1 could partially restore inflorescence-branching phenotypes caused by high STM3 expression in ST024. (nature.com)

While a few important genes for tomato inflorescence-branching development have been identified, the regulatory mechanism underlying inflorescence branching is still unclear. (nature.com)
A series of regulatory genes that have received much attention makes major contributions to inflorescence architecture in tomato by changing the inflorescence-branching pattern. (nature.com)
We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
First, PCR-amplified marker genes are limited to a narrow subset of the viral community, and require degeneracies and amplification conditions that undermine the quantitative nature of the data ( Sullivan, 2015 ). (peerj.com)
Nucleic acid sequences involved in regulating the expression of genes. (lookformedical.com)
Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)

No. 63/298,867, filed Jan. 12, 2022, entitled "CASCADE OLIGONUCLEOTIDE DISPLACEMENT PROBES," the entire contents of which are incorporated herein by reference. (justia.com)

Furthermore, the use of Taq or other thermostable DNA polymerases permits labeling reactions to be performed at higher temperatures via PCR, thereby reducing the incidence of enzyme-mediated point mutations during probe synthesis. (enzolifesciences.com)
It involves randomly nicking the backbone of a double-stranded DNA with dilute concentrations of DNase I. At extremely low concentrations, this enzyme nicks a template at four or five sites, producing a free 3′-OH group that can act as a primer at each nicking location. (enzolifesciences.com)
Trichothecenes are produced by a sequence of oxygenations, isomerizations, cyclizations, and esterifications leading from trichodiene, which is produced from the cyclization of trans, trans-farnesyl pyrophosphate by the enzyme trichodiene synthase (Desjardins, Hohn, and McCormick, 1993, Microbiological Reviews 57: 595-604). (justia.com)
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. (lookformedical.com)
The enzyme has a preference for double-stranded DNA. (lookformedical.com)

Activation of Hedgehog signaling by the environmental toxicant arsenic may contribute to the etiology of arsenic-induced tumors. (mbl.edu)

The disclosure is based, in part, on methods and compositions that produce nucleic acid reporters that result in high contrast, visible, signals (e.g., colored bands) when bound to certain immunoassay devices (e.g., lateral flow immunoassays). (justia.com)
In some embodiments, compositions and methods described herein allow for direct application of amplification reactions including the nucleic acid reporters to immunoassay devices without any intervening fluid transfer or dilution steps. (justia.com)

Nick translation is efficient for both linear and covalently closed DNA molecules, and labeling reaction are completed in less than an hour. (enzolifesciences.com)
The performance of each assay was monitored by running a dilution series (1 x 102 to 1 x 108 copies per reaction) of E. coli genomic DNA in TaqMan™ Fast Advanced Master Mix, following manufacturers recommendations for assay concentrations and cycling conditions. (biosearchtech.com)
The poly(A) tail is encoded in the DNA template, which eliminates reaction steps and reduces overall production time and material loss [19] . (researchgate.net)

All of these viruses, and the ancestral Bulgarian ( n = 2) and South Korean ( n = 2) viruses described here, have predicted virulent cleavage sites of the fusion protein, and those selected for further characterization have intracerebral pathogenicity index assay values characteristic of NDV of genotype VI (1.31 to 1.48). (biomedcentral.com)

It is located in an intronic sequence of the DGKB gene on chromosome 7 and is highly conserved in Hominidae (the great apes), but absent from Hylobatidae (gibbon and siamang). (biomedcentral.com)

Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample. (ctsicn.org)

Caretti E, Devarajan K, Coudry R, Ross E, Clapper ML, Cooper HS, Bellacosa A. Comparison of RNA amplification methods and chip platforms for microarray analysis of samples processed by laser capture microdissection. (jefferson.edu)
METHODS: We retrospectively selected 18 cancers (13 endometrial cancers, 2 brain tumors, 1 ovarian cancer, 1 lung cancer, and 1 cancer of unknown origin) harboring oncogenic PTEN missense mutations, which were confirmed by targeted next-generation sequencing. (bvsalud.org)
The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. (justia.com)
The 'VirION' (Viral, long-read metagenomics via MinION sequencing) approach was first validated using mock communities where it was found to be as relatively quantitative as short-read methods and provided significant improvements in recovery of viral genomes. (peerj.com)

LAM is composed of a mannan backbone with branched arabinosyl chains. (biomedcentral.com)
DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine). (lookformedical.com)

EMSA and dual-luciferase reporter assays further confirmed that STM3 could directly bind the promoter region to activate FUL1 expression. (nature.com)
Here we have investigated nc886 in gastric cancer where its expression is suppressed due to CpG DNA hypermethylation at its promoter region in a cohort of paired tumor/normal tissues from 88 gastric cancer patients. (oncotarget.com)

DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)

Random priming is a type of primer extension in which a mixture of small oligonucleotide sequences, acting as primers, anneal to a heat-denatured double-stranded template. (enzolifesciences.com)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)
In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)

Complementary testing using both immunostaining and next-generation sequencing should be conducted to accurately evaluate the PTEN status in malignancy. (bvsalud.org)

Flow Cytometry RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to stimulus. (technion.ac.il)
Development of the PrimeFlow™ RNA Assay is based upon proven and well-published ViewRNA™ technology designed for microscopic analysis of RNA in cells and tissues. (technion.ac.il)
Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. (uchicago.edu)
RNA Sanger sequencing analysis was performed in AEG‑1‑deficient HT22 cells and wild‑type HT22 cells without knockout (KO). (spandidos-publications.com)

High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. (cdc.gov)
Here we established a low-cost, low-input, high throughput alternative sequencing and informatics workflow to improve viral metagenomic assemblies using short-read and long-read technology. (peerj.com)

PtrABF had a complete open reading frame of 1347 bp, encoding a 448 amino acid peptide, and shared high sequence identities with ABFs from other plants. (biomedcentral.com)
The structural integrity of the N-terminus is sensitive to changes in amino acid sequence surrounding N2 and possibly dependent on the presence of the carbohydrate moiety itself at N15 ( Tam and Moritz, 2009 ). (jneurosci.org)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

For example, early reports of the imaging of DNA in scanning tunneling microscopes (STMs) used highly ordered pyrolitic graphite (HOPG) substrate, and subsequent investigations showed that at least some of the features reported were not those of DNA at all but of artifacts on the HOPG surface. (nationalacademies.org)

Two micrograms of DNaseI-treated RNA was reverse transcribed by using Superscript II (Invitrogen, Carlsbad, CA, USA) and random octamer primers with an arbitrary specific anchor sequence as described previously ( 9 ). (cdc.gov)
CDC has shared the primers and probes sequence specifications so that laboratories and test developers may manufacture their own reagents. (cdc.gov)
Comparison of RT-LAMP assay in saliva purified RNA vs. Raw saliva diagnosed with a pair set of primers. (bjid.org.br)

These assays are 97% specific but cannot distinguish acute from chronic infection. (medscape.com)
Using the PrimeFlow™ RNA Assay, specific cell populations may be analyzed for unique transcript expression levels or cell subsets evaluated over time to determine transcriptional regulation and protein expression simultaneously. (technion.ac.il)
Thereby, both cascades appear to follow a stage-specific sequence. (biomedcentral.com)

The recombinant immunoblot assay is used to confirm HCV infection. (medscape.com)
Due to its high sensitivity, this technique has widely been used to discover low amounts of pathogen DNA in the environment or in an organism during infection, to monitor its spread as well as to study healthy carriers as pathogen reservoirs [22-24]. (peptidescost.com)
Any positive HIV DNA PCR finding should be confirmed with follow-up HIV DNA PCR before infection is diagnosed. (medscape.com)

Evaluation of a laboratory-developed multiplex real-time PCR assay for diagnosis of syphilis, herpes and chancroid genital ulcers in four public health laboratories in the USA. (cdc.gov)
However, due to the high cost and delay of the results, other types of diagnosis were implemented, such as COVID-19 Ag Rapid Tests and Reverse Transcription Technique followed by Loop-Mediated isothermal Amplification (RT-LAMP). (bjid.org.br)

Like DNA, strands of RNA contain genetic data. (elifesciences.org)

Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Combined with a non-fluorescent quencher, such as EDQ, the short MGB Probe leads to low background and high signal-to-noise. (biosearchtech.com)
At steady state a given fluorescent signal is generated. (trea.com)

DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. (lookformedical.com)

Our results demonstrate that the global probe selection approach based on longest common factor statistics as well as the design of a DNA microarray with a restricted set of discriminative probes enables robust discrimination of different enterobacterial variants and represents a proof of concept that can be adopted for diagnostics of a wide range of microbial pathogens. (biomedcentral.com)

Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. (lookformedical.com)

High expression levels of STM3 underlie the highly inflorescence-branching phenotype in ST024. (nature.com)
This is primarily because Alu elements do not contain coding sequence and the vast majority of insertions are highly similar to each other. (biomedcentral.com)

In addition, upregulation of AEG-1 expression can protect nigral dopaminergic neurons from injury caused by aberrant apoptotic signaling pathways ( 13 ). (spandidos-publications.com)

However in contrast to heterogeneous immunoassays, homogeneous immunoassays have problems in signal generation, which so far results in less sensitive assays. (trea.com)

MRI showed that anterior mediastinal mass showed intermediate signal intensity on T1-weighted images (T1WI), high SI on T2-weighted images (T2WI), and heterogeneous enhancement. (bvsalud.org)
CONCLUSION: Thymic mucinous adenocarcinomas could be included in differential diagnoses of anterior mediastinal tumors showing extensive calcification, and common imaging findings of mucinous adenocarcinoma such as T2 high signal intensity and heterogeneous enhancement on MRI may be helpful to diagnose thymic mucinous adenocarcinoma. (bvsalud.org)

MGB assays were designed for the E. coli gene lacZ. (biosearchtech.com)
coli was also DNA Damage inhibitor tested and compared to that of the wild type E. coli. (peptidescost.com)

STM3 is expressed in both vegetative and reproductive meristematic tissues and in leaf primordia and leaves, indicative of its function in flowering time and inflorescence-branching development. (nature.com)

DNA cleavage is inhibited where the ligand binds to DNA. (lookformedical.com)
A cytotoxic polypeptide quinoxaline antibiotic isolated from Streptomyces echinatus that binds to DNA and inhibits RNA synthesis. (lookformedical.com)

The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION. (lookformedical.com)

Favorable inflorescence branching is always a major breeding target for achieving desirable production by balancing the sink-source relationship. (nature.com)
Immune checkpoint inhibitors (ICIs), widely indicated in the treatment of several malignancies, target inhibitory receptors on T lymphocytes, including the programmed cell death protein (PD-1)/programmed death-ligand 1 (PD-L1) axis and cytotoxic T-lymphocyte-associated protein 4 (CTLA4), and release effector T-cells from negative feedback signals. (bvsalud.org)
back, so of the book stéréochimie molécules en of hunting, breath of revolution, scoring generally to study comparison or to target been theories, may study excellent against manuring or PMDs. (firefox-gadget.de)

Neuronal responsiveness to the neurotrophins is determined not only by restricted expression and regulated release of the neurotrophins but also by the expression pattern of neurotrophin receptors and components of the signaling complex. (jneurosci.org)

Different tomato varieties require distinct inflorescence-branching structures to enhance productivity. (nature.com)
These assays reach good sensitivities, but require washing steps, which makes them complicated and slow. (trea.com)

Chemicals and Drugs4

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes3

Information Science7

Health Care1

Destroying the invading nuc1

Labeled probes2

BDNA7

Microarrays3

Specificity4

Whole-genome3

Nucleic acid sequence2

Pathway2

Genome4

Polymerase4

Detection6

Single stranded4

Nucleotide sequence2

Bacterial1

Antibody3

Clinical4

Enhanced-sensitivity1

Receptor5

Colorimetric2

Specimens1

Proteins4

Methylation1

Detect1

Mutation1

Genes6

20221

Enzyme5

Hedgehog1

Immunoassay2

Reaction3

Genotype1

Chromosome1

Molecules1

Methods4

Backbone2

Promoter region2

Diagnostic2

Double-stranded1

Nucleotides2

Complementary1

Analysis4

Viral2

Amino3

Subsequent1

Primers3

Specific3

Infection3

Diagnosis2

Genetic1

Fluorescent3

Nuclease1

Pathogens1

Eukaryotes1

Highly2

Neurons1

Contrast1

High2

Coli2

Development1

Binds2

Transcription1

Target3

Expression1

Require2