• Dos genes mais expressos nos tumores dos indivíduos portadores de diabetes melito, três deles, FAM3D, do inglês Family with Sequence Similarity number 3 member D, neuropeptídeo Y (NPY), e proteína de ligação do cálcio S100A8, foram estudados por reação em cadeia da polimerase em tempo real. (usp.br)
  • Three genes were chosen for RT-qPCR: Family with Sequence Similarity number 3 member D (FAM3D), neuropeptide Y (NPY), and calcium-binding protein S100A8. (usp.br)
  • Setenta genes mais diferencialmente expressos (expressão aumentada ou reduzida) foram selecionados a partir dos diferentes tratamentos e várias redes moleculares foram identificadas utilizando o Ingenuity Pathway Analysis (IPA). (usp.br)
  • This analysis unravels six clusters of genes showing no significant variation in mRNA expression levels whereas they are submitted to translational regulation. (cnrs.fr)
  • Fine-scale analysis of rare variants in XLID families leads us to propose four additional genes, PTCHD1, WDR13, FAAH2, and GSPT2, as candidates for XLID causation and the identification of further deletions and duplications affecting X chromosome genes but without apparent disease consequences. (edu.au)
  • High frequency genes were subjected to survival analyses. (springer.com)
  • Accumulated data from high-throughput analyses by cDNA microarray provide an accurate landscape of gene expression in HCC, and revealed a lot of pathogenic and prognostic genes for HCCs, thus enabled us to delineate some of the key events that might dominate tumor development and progression. (springer.com)
  • This provides a relatively low-cost microarray that may be customized for each study, and avoids the costs of purchasing often more expensive commercial arrays that may represent vast numbers of genes that are not of interest to the investigator. (wikipedia.org)
  • Through DNA microarray analysis and quantitative PCR verification, we have identified additional IL-17A-inducible genes-IL-19, CXCL-1, -2, -3, -5, and -6 - in well-differentiated normal human bronchial epithelial cells. (elsevier.com)
  • Researches have been conducted for the identification of differentially expressed genes (DEGs) by generating and mining of cDNA expressed sequence tags (ESTs) for more than a decade. (biomedcentral.com)
  • Here we presented a different approach which searched for multi-cancer DEG candidates by analyzing all pertinent ESTs in all categories and narrowing down the cancer biomarker candidates via integrative analysis with microarray data and selection of secretory and membrane protein genes as well as incorporation of network analysis. (biomedcentral.com)
  • The Affymetrix ATH1 array provides a robust standard tool for transcriptome analysis, but unfortunately does not represent all of the transcribed genes in Arabidopsis thaliana. (mpg.de)
  • With the tiling arrays we could identify 368 new E2F-regulated genes, with a large fraction including an E2F motif in the promoter. (mpg.de)
  • Functional genomics, broadly defined as the comprehensive analysis of genes and their products, have become a recent focus of the life sciences ( 1 ). (mcponline.org)
  • DNA microarrays (cDNA and oligonucleotide) permit the parallel assessment of thousands of genes and have been utilized in gene expression monitoring ( 7 ), polymorphism analysis ( 8 ), and DNA sequencing ( 9 ). (mcponline.org)
  • We hypothesized that for human and mouse, many orthologous genes expressed in a similarly tissue-specific manner in both human and mouse gene expression data, are likely to be co-regulated by orthologous TFs that bind to DNA sequence motifs present within noncoding sequence conserved between these genomes. (biomedcentral.com)
  • Here, we applied a genome wide microarray based expression analysis to identify DDIT3 target genes and functions. (gu.se)
  • Promoters of target genes showed no common sequence motifs, reflecting that DDIT3 forms heterodimers with several alternative transcription factors that bind to different motifs. (gu.se)
  • In this study, we utilize fluorescent activated cell sorting (FACS) of cells from transgenic zebrafish coupled with microarray analysis to globally analyze expression of cell type specific genes. (umassmed.edu)
  • Microarray analysis of GFP+ versus GFP- cells isolated from Tg(fli1:egfp)(y1) embryos identifies genes expressed in hematopoietic, vascular and pharyngeal arch tissue, consistent with the expression of the fli1:egfp transgene in these cell types. (umassmed.edu)
  • Our results demonstrate that this is a robust approach for identification of cell type specific genes as well as for global analysis of cell type specific gene expression in zebrafish embryos. (umassmed.edu)
  • Probands from eight families with at least two affected individuals were screened by direct sequencing of 27 candidate genes. (qub.ac.uk)
  • Combined analysis of gene expression array data and array-based comparative genomic hybridization data have been used in a series of 26 pediatric brain tumors to define up- and downregulated genes that coincide with losses, gains, and amplifications involving specific chromosome regions. (elsevier.com)
  • To facilitate the analysis of the two data sets, we have developed a custom overlay tool that defines genes that are underexpressed in regions of deletions and overexpressed in regions of gain, across the genome and specifically within regions showing recurrent involvement in medulloblastomas. (elsevier.com)
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • The results of cDNA expression array revealed up-regulation of cell cycle inhibitory genes such as cyclin-dependent kinase 4 inhibitor, and interferon-related genes such as STAT2 and 3, while down-regulation of cyclin-dependent kinase 2 and cyclin E. Up-regulated production of p21WAF-1 and Mcl-1/EAT was also confirmed by Western blotting. (elsevier.com)
  • In this study, transcriptional profiles of these cell lines were created using oligonucleotide microarrays representing over 12,000 genes. (elsevier.com)
  • Tumor tissue is also evaluated by real-time polymerase chain reaction to confirm drug effects on expected targets and on EGFR expression, activity, and affected signaling pathways in the disease state and by microarray analysis to define expression phenotypes correlating with outcome, distinguish responders from nonresponders, and determine effects of drug treatment on gene expression in disease. (bioportfolio.com)
  • Emerging genetic sequence data and phenotype association studies are expected to enable disease risk prediction and guide subsequent therapeutic approaches in individual cases. (aspetjournals.org)
  • Intensity modeling and hierarchical clustering analysis identified a 171-gene expression signature that correlated with metastatic phenotype and highlighted several GTPase signaling components. (elsevier.com)
  • Gene expression in surgically resected and microdissected samples of non-small-cell lung cancers (18 squamous cell carcinomas and nine adenocarcinomas), matched normal bronchial epithelium, and peripheral lung tissue from both smokers (n = 22) and non-smokers (n = 5) was studied using the Affymetrix U133A array. (uni-regensburg.de)
  • Later, Affymetrix developed Modified Partitioning Around Medoids (MPAM) [ 19 ] as the default algorithm for analysis of the 10K chip. (biomedcentral.com)
  • Using high-density oligonucleotide array chip (Affymetrix 6.0) to perform parental and proband samples concurrently on three chips and interpreted as a trio set, a de novo 3.2Mb deletion from bands q12.2 to q13 on chromosome 16 (from 52.08 to 55.3Mb) of paternal origin was identified. (elsevier.com)
  • The results from the array studies highlighted the complexity of rearrangements of chromosome 22 compared with the low frequency of alterations involving the other chromosomes. (aacrjournals.org)
  • Our new capability to broadly define human variability at the genetic level and to associate genetic variability with functional diversity has largely been enabled by this recent technical revolution, including large scale functional genomic, genetic, and proteomic analyses. (aspetjournals.org)
  • By combining proteomic and transcriptional analyses of the same samples, however, it may be possible to understand the complex mechanisms influencing protein expression in human cancer. (mcponline.org)
  • Tumor tissue is obtained at baseline (at the original or confirmatory transurethral resection of the bladder tumor) and at the time of cystectomy for analysis of drug-specific and tissue-based biomarkers by western blot, immunohistochemistry, and gene array techniques. (bioportfolio.com)
  • Combined data from OA's expression array and function prediction data suggest that OA might play a role in several pathologic conditions, including hypertension, diabetes, and immune system disorders. (medscimonit.com)
  • This project will develop methods to utilize new highly parallel DNA sequencing platforms to investigate structural variations in the genomes of cancer cells. (genome.gov)
  • Next-generation sequencing technologies are now being exploited not only to analyse static genomes, but also dynamic transcriptomes in an approach termed RNA-seq. (ucl.ac.uk)
  • Net reclassification improvement analysis was performed to compare the prognostic accuracy of GEP with the 7th edition clinical Tumor-Node-Metastasis (TNM) classification and chromosome 3 status. (cdc.gov)
  • This chapter covers amplified- and nonamplified-probe techniques, postamplification detection and analysis, clinical applications of these techniques. (asmscience.org)
  • The expression and prognostic value of alanine-glyoxylate and serine-pyruvate aminotransferase (AGXT) was further evaluated in HCC datasets in Oncomine and an independent HCC tissue array cohort. (springer.com)