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  • Genes
  • Dos genes mais expressos nos tumores dos indivíduos portadores de diabetes melito, três deles, FAM3D, do inglês Family with Sequence Similarity number 3 member D, neuropeptídeo Y (NPY), e proteína de ligação do cálcio S100A8, foram estudados por reação em cadeia da polimerase em tempo real. (usp.br)
  • Three genes were chosen for RT-qPCR: Family with Sequence Similarity number 3 member D (FAM3D), neuropeptide Y (NPY), and calcium-binding protein S100A8. (usp.br)
  • Setenta genes mais diferencialmente expressos (expressão aumentada ou reduzida) foram selecionados a partir dos diferentes tratamentos e várias redes moleculares foram identificadas utilizando o Ingenuity Pathway Analysis (IPA). (usp.br)
  • We performed microarray analysis to identify genes overexpressed in 7 metastatic lymph nodes from OSCC patients, compared to 1 normal lymph node and 5 salivary glands from non-cancer patients. (cancerindex.org)
  • Of 4 genes identified by microarray analysis, annexin A8 (ANXA8) and desmoglein 3 mRNA were detected by qRT-PCR in metastatic lymph nodes but not in normal lymph nodes. (cancerindex.org)
  • Microarray analysis of differentially expressed genes regulating lipid metabolism during melanoma progression. (cancerindex.org)
  • Therefore, we used BRB (Biometrics Research Branch) class comparison tool with multivariate analysis to identify differentially expressed genes in human cutaneous melanomas, compared with benign nevi and normal skin derived from the microarray dataset (GDS1375). (cancerindex.org)
  • This provides a relatively low-cost microarray that may be customized for each study, and avoids the costs of purchasing often more expensive commercial arrays that may represent vast numbers of genes that are not of interest to the investigator. (wikipedia.org)
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • Analysis of custom genomic regions of interest (e.g. specific genes, multiple variants and/or complete pathways). (wikipedia.org)
  • Synthetic biology) An example of a recent application is testing of the BRCA1 and BRCA2 breast cancer risk genes The FlexArrayer is an in-house custom oligonucleotide synthesis instrument. (wikipedia.org)
  • In 2011, the genome of an exceptional bladder cancer patient whose tumor had been eliminated by the drug everolimus was sequenced, revealing mutations in two genes, TSC1 and NF2. (wikipedia.org)
  • A computational screen based on promoter sequences recognised by the sigma factor sigma 70 and on Rho-independent terminators predicted 24 putative sRNA genes, 14 of these were verified experimentally by northern blotting. (wikipedia.org)
  • From this set of candidate genes, microarray analysis and northern blotting confirmed the existence of 17 previously undescribed sRNAs, many of which bind to the chaperone protein Hfq and regulate the translation of RpoS (Sigma 38). (wikipedia.org)
  • Esr41 sequence is not present in nonpathogenic E. coli K12, but the sRNA can induce cell motility in K12 as well, suggesting that target genes controlled by Esr41 are present in both E.coli. (wikipedia.org)
  • PROBES
  • Each DNA spot contains picomoles (10−12 moles) of a specific DNA sequence, known as probes (or reporters or oligos). (wikipedia.org)
  • Many types of arrays exist and the broadest distinction is whether they are spatially arranged on a surface or on coded beads: The traditional solid-phase array is a collection of orderly microscopic "spots", called features, each with thousands of identical and specific probes attached to a solid surface, such as glass, plastic or silicon biochip (commonly known as a genome chip, DNA chip or gene array). (wikipedia.org)
  • Arrays from commercial vendors may have as few as 10 probes or as many as 5 million or more micrometre-scale probes. (wikipedia.org)
  • The probes are synthesized prior to deposition on the array surface and are then "spotted" onto glass. (wikipedia.org)
  • A common approach utilizes an array of fine pins or needles controlled by a robotic arm that is dipped into wells containing DNA probes and then depositing each probe at designated locations on the array surface. (wikipedia.org)
  • These arrays may be easily customized for each experiment, because researchers can choose the probes and printing locations on the arrays, synthesize the probes in their own lab (or collaborating facility), and spot the arrays. (wikipedia.org)
  • Current cancer research makes use primarily of DNA microarrays in which an arrayed series of microscopic spots of pre-defined DNA oligonucleotides known as probes are covalently attached to a solid surface such as glass, forming what is known as a gene chip. (wikipedia.org)
  • After amplifying one DNA oligoprobe of interest, it can be used to create 100 different probes on 100 different sets of microspheres, each with the capability of capturing 100 potential targets (if using a 100-plex array). (wikipedia.org)
  • microarrays
  • Play media Microarrays can be fabricated using a variety of technologies, including printing with fine-pointed pins onto glass slides, photolithography using pre-made masks, photolithography using dynamic micromirror devices, ink-jet printing, or electrochemistry on microelectrode arrays. (wikipedia.org)
  • DNA microarrays evolved from Southern blotting which allows for detection of a specific DNA sequence in a sample of DNA. (wikipedia.org)
  • High array density/multiplex: Compared to flat microarrays, SAT allows one to perform parallel measurements. (wikipedia.org)
  • microscopic
  • The alternative bead array is a collection of microscopic polystyrene beads, each with a specific probe and a ratio of two or more dyes, which do not interfere with the fluorescent dyes used on the target sequence. (wikipedia.org)
  • siRNA
  • The program is a comprehensive real time PCR primer and probe search and analysis tool, and also does other tasks such as siRNA and molecular beacon searches, open reading frame and restriction enzyme analysis etc. (wikipedia.org)
  • bioinformatics
  • John D. Offerman, Wojciech Rychlik (2003) Oligo Primer Analysis Software in Introduction to bioinformatics: a theoretical and practical approach. (wikipedia.org)
  • These experimental and bioinformatics analyses favor the hypothesis that exRNAs play a role in numerous biological processes. (wikipedia.org)
  • high-throughput
  • In this study, we used high-throughput phosphorylation array to test two pancreatic cancer cell lines (PC-1 cells with a low, and PC-1.0 cells with a high potential for invasion and metastasis). (cancerindex.org)
  • Suspension array technology (or SAT) is a high throughput, large-scale, and multiplexed screening platform used in molecular biology. (wikipedia.org)
  • Rapid/high throughput: In multiplex analysis, a 100-plex assay can be analyzed in every 30 seconds. (wikipedia.org)
  • In 2010 BGI Shenzhen was certified as meeting the requirements of ISO9001:2008 standard for the design and provision of high-throughput sequencing services, The same year BGI bought 128 sequencing machines and claimed to be the world's largest genome center. (wikipedia.org)
  • coli
  • Deep sequencing of RNA expressed during chemical stress and high cell density fermentation discovered 253 novel intergenic transcripts adding to roughly 200 intergenic sRNAs previously described in E. coli. (wikipedia.org)
  • BGI did the genome sequencing for the deadly 2011 Germany E. coli O104:H4 outbreak in three days under open licence. (wikipedia.org)
  • Genomics
  • The genomics era began in the 1990s, with the generation of DNA sequences of many organisms. (wikipedia.org)
  • pinyin: Huádà Jīyīn), known as the Beijing Genomics Institute prior to 2008, is one of the world's genome sequencing centers, headquartered in Shenzhen, Guangdong, China. (wikipedia.org)
  • That year it bought Complete Genomics of Mountain View, California, a major supplier of DNA sequencing technology, for US$118 million. (wikipedia.org)
  • computational
  • Here, we performed a computational analysis that integrated time-course EMT transcriptomic data with public cistromic data and identified three synergistic master TFs (ETS2, HNF4A and JUNB) that regulate the transition through the partial-EMT state. (cancerindex.org)