• A novel approach to tritium-labeled antisense oligonucleotides (ASO) was established by conjugating N -succinimidyl propionate, as well as maleimide-derivatives, to the 3′-end of ASOs targeting metastasis-associated lung adenocarcinoma transcript 1 (Malat1) containing amino- or sulfhydryl-linkers. (springeropen.com)
  • Using viral RNA, Stephenson and Zamecnik ( 1978 ) demonstrated in 1978 that translation of proteins could be efficiently inhibited in vitro by competitive hybridization with antisense oligonucleotides (ASO). (springeropen.com)
  • An interesting addition to the protection of antisense oligonucleotides is to modify the terminal linkages from the natural 3'-5' to 3'-3' and/or 5'-5' linkages. (biosearchtech.com)
  • Steidl U, Haas R, Kronenwett R. Intercellular adhesion molecular 1 on monocytes mediates adhesion as well as trans-endothelial migration and can be downregulated using antisense oligonucleotides. (jamanetwork.com)
  • [1] Uhlmann E, Peyman A. Antisense oligonucleotides: a new therapeutic principle. (org.ua)
  • Antisense oligodeoxynucleotides (AS-ODNs) have been widely used to determine gene function, validate drug targets and as novel therapeutics for human diseases. (uni-regensburg.de)
  • Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs. (elsevierpure.com)
  • We investigated about targeting for new antimalarial drugs using antisense (AS) oligodeoxynucleotides (ODNs). (elsevierpure.com)
  • Dive into the research topics of 'Antisense oligodeoxynucleotides: useful tool for search and assessment of new targets for anti-malarial drugs. (elsevierpure.com)
  • a) Antisense effect of oligodeoxynucleotides with inverted terminal internucleotidic linkages: a minimal modification protecting against nucleolytic degradation, J.F.R. Ortigao, H. Rosch, H. Selter, A. Frohlich, A. Lorenz, M. Montenarh and H. Seliger, Antisense Res. (biosearchtech.com)
  • Decrease in TGF-β2 expression in LCC2 cells was achieved by treatment with antisense oligodeoxynucleotides and confirmed by TGF-β2 immunoblot analysis. (elsevierpure.com)
  • Antisense TGF-β2 oligodeoxynucleotides enhanced the NK sensitivity of LCC2 cells in the presence of tamoxifen. (elsevierpure.com)
  • To this goal we used Lyn and Raf-1 antisense (AS) oligodeoxynucleotides (ODN) to inhibit the expression of these proteins and tyrphostin AG490 to specifically block the activation of Jak2. (utmb.edu)
  • b) Oligonucleotide analogs with terminal 3'-3'-internucleotidic and 5'-5'-internucleotidic linkages as antisense inhibitors of viral gene-expression, H. Seliger, A. Frohlich, M. Montenarh, J.F.R. Ortigao and H. Rosch, Nucleosides & Nucleotides, 10, 469-477, 1991. (biosearchtech.com)
  • Oligodeoxyribonucleotides" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • In the present study, we designed an antisense oligodeoxynucleotide of DNMT1 (AS/MT: 5′-CGGTAC GCGCCGGCATCT-3′) and demonstrated successful inhibition of DNMT1 expression by AS/MT at the protein level, using gastric cancer cell lines in vitro. (elsevierpure.com)
  • Incubation of PAEC with an antisense oligodeoxyribonucleotide of calpastatin prevented CSE-induced increases in calpastatin protein and CSE-induced decreases in calpain activity, eNOS gene transcription, activity and protein content of eNOS, and NO release. (elsevierpure.com)
  • Kronenwett R, Steidl U, Kirsch M, Sczakiel G, Haas R. Oligodeoxyribonucleotide uptake in primary human hematopoietic cells is enhanced by cationic lipids and depends on the hematopoietic cell subset. (jamanetwork.com)
  • This graph shows the total number of publications written about "Oligodeoxyribonucleotides" by people in this website by year, and whether "Oligodeoxyribonucleotides" was a major or minor topic of these publications. (umassmed.edu)
  • They found that silencing BrFER1 or BrANJ1 resulted in inhibition of the ROS increase, and compromised SI and UI pollen rejection, which was consistent with the phenotype observed in plants with BrRBOHF silenced by antisense technology (AS-BrRBOHF). (biomedcentral.com)
  • In conclusion, the inhibition of DNA methylation by DNMT1 by an antisense oligodeoxynucleotide influences cell morphology and adhesion, as well as cell growth in gastric cancer cells in vitro. (elsevierpure.com)
  • Objective: We investigated the association between zif268 and the extinction of conditioned fear by using antisense oligodeoxynucleotide (ODN) of zif268 and the fear-potentiated startle paradigm. (ntnu.edu.tw)
  • Targeted approaches for gene knockdown emerged in the 1980s with techniques such as homologous recombination, trans-cleaving ribozymes, and antisense technologies. (wikipedia.org)
  • Notably, siRNA suppress non-targeted genes due to (1) the partial complementarity to mRNA 3'-untranslated regions (3'-UTR), and (2) the antisense activity of the sense strand. (thno.org)
  • a) Antisense effect of oligodeoxynucleotides with inverted terminal internucleotidic linkages: a minimal modification protecting against nucleolytic degradation, J.F.R. Ortigao, H. Rosch, H. Selter, A. Frohlich, A. Lorenz, M. Montenarh and H. Seliger, Antisense Res. (biosearchtech.com)
  • Intra-amygdaloid injection of the antisense ODN of zif268 blocked the facilitation effect of DCS on the extinction of conditioned fear. (ntnu.edu.tw)
  • Subsequent control experiments indicated that administration of vehicle or zif268 sense ODN did not alter the facilitation of DCS and that the blockage effect of zif268 antisense ODN was not due to lasting damage to the amygdala. (ntnu.edu.tw)
  • Sato W, Takei Y, Yuzawa Y, Matsuo S, Kadomatsu K, Muramatsu T. Midkine antisense oligodeoxyribonucleotide inhibits renal damage induced by ischemic reperfusion. (ac.ir)
  • Methods: Male adult Wistar rats were injected DCS (15 mg/kg, IP) 15 min prior to the extinction training, administered with antisense or sense ODN (800 pmol) of zif268 and subjected for fear-potentiated startle paradigm (FPS) and Western blot. (ntnu.edu.tw)
  • Therefore, the reader who lacks familiarity with genetic techniques would do well to read the paper by Tao and Johns carefully because it provides an excellent description of rationale for use of antisense oligodeoxyribonucleotides and is an excellent example of the appropriate way to use them as research tools. (silverchair.com)