• Studies showed that the phosphorylation state of OLIG2 at Ser30 determines the fate of cortical progenitor cells, in which cortical progenitor cells will either differentiate into astrocytes or remain as neuronal progenitors. (wikipedia.org)
  • Knock-down of chick Shh did not affect the proliferation (BrdU) or survival (cCasp3) of neural progenitor cells and ventral neural patterning (Olig2 and Nkx2.2). (elifesciences.org)
  • Hafler B.P., Surzenko N., Beier K.T., Punzo C ., Trimarchi J.M., Kong J.H., Cepko C.L. (2012) Transcription factor Olig2 defines subpopulations of retinal progenitor cells biased toward specific cell fates. (umassmed.edu)
  • Recombinant protein encompassing a sequence within the N-terminus region of human OLIG2. (thermofisher.com)
  • We next localized SLC7A10 protein by immunohistochemistry in the lateral globus pallidus, which was also genetically labeled for Olig2. (biomedcentral.com)
  • SLC7A10 protein was also immunolabeled in the Olig2-astrocytes of the LGP, especially in their fine processes. (biomedcentral.com)
  • A lately developed and validated immunotagging technique makes use of three transcription issue antibodies to concurrently isolate enriched neuronal (NeuN+), astrocyte (paired field protein 6 (PAX6)+NeuN-), and oligodendrocyte progenitor ( OLIG2 +NeuN-) nuclei populations from non-diseased, recent (unfixed) snap-frozen postmortem human temporal neocortex tissue. (aetox.com)
  • Protein expression of OLIG2 , a novel marker in RMS, was investigated utilizing antibody EP112 (Cell Marque). (aetox.com)
  • Phosphorylation at a triple serine motif (Ser10, Ser13 and Ser14) on the other hand was shown to regulate the proliferative function of OLIG2. (wikipedia.org)
  • It is further found that the phosphorylation of triple-serine motif in OLIG2 is present in several glioma lines and is more tumorigenic than the unphosphorylated status. (wikipedia.org)
  • Both SUMOylation and triple serine motif (TSM) phosphorylation of Olig2 are required for the antiapoptotic function. (climat2050.org)
  • OLIG2 overexpression is a good surrogate marker for IDH mutation with an AUC of 0.90, but predicts poorly (AUC = 0.55) for 1p/19q co-deletion, a class-defining chromosomal alteration for oligodendroglioma. (wikipedia.org)
  • OLIG2 is well known for determining motor neuron and oligodendrocyte differentiation, as well as its role in sustaining replication in early development. (wikipedia.org)
  • During embryogenesis, OLIG2 first directs motor neuron fate by establishing a ventral domain of motor neuron progenitors and promoting neuronal differentiation. (wikipedia.org)
  • OLIG2 then switches to promoting the formation of oligodendrocyte precursors and oligodendrocyte differentiation at later stages of development. (wikipedia.org)
  • Apart from functioning as a neurogenic factor in specification and the differentiation of motor neurons and oligodendrocytes, OLIG2 also functions as an anti-neurogenic factor at early time points in pMN progenitors to sustain the cycling progenitor pool. (wikipedia.org)
  • The role of phosphorylation has been highlighted recently to account for the multifaceted functions of OLIG2 in differentiation and proliferation. (wikipedia.org)
  • 2013 ) Olig2 targets chromatin remodelers to enhancers to initiate oligodendrocyte differentiation. (academictree.org)
  • Oligodendrocyte transcription factor (OLIG2) is a basic helix-loop-helix (bHLH) transcription factor encoded by the OLIG2 gene. (wikipedia.org)
  • We have reported that the transcription factor Olig2 labels a subpopulation of astrocytes (Olig2-astrocytes), which show distribution patterns different from those of GFAP-expressing astrocytes (GFAP-astrocytes) in the adult brain. (biomedcentral.com)
  • OLIG2 (Oligodendrocyte transcription factor-2) is a transcription factor that is expressed in the pMN domain of the ventral ventricular zone in the embryonic spinal cord . (shu.edu)
  • In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, in human embryonic stem cell (hESC) line BG01 by homologous recombination. (ca.gov)
  • C ) Quantification of the relative intensity of Shh ISH signal in motor neurons, % marker + (BrdU, Nkx2.2, Olig2, and cCasp3) cells relative to uninjected side and % motor columns relative to uninjected side of the spinal cord. (elifesciences.org)
  • In a study using the U12-1 cell line for controlled expression of OLIG2, researchers showed that OLIG2 can suppress the proliferation of U12-1 by transactivating the p27Kip1 gene and can inhibit the motility of the cell by activating RhoA. (wikipedia.org)
  • Unbiased classification of gene expression profiles and subsequent gene ontology analyses revealed that the majority of Olig2-astrocytes belonged to an astrocytic cluster that is enriched for transporter-related genes. (biomedcentral.com)
  • To complement the in silico data analyses, we differentially isolated Olig2- and GFAP-astrocytes from the same frozen section of the lateral globus pallidus using laser microdissection and compared their gene expression by quantitative reverse transcription PCR. (biomedcentral.com)
  • The Olig2 gene thus seems to play a role in generating astrocytic heterogeneity, and the regional heterogeneity of Olig2-astrocyte distribution implies that each subpopulation has specific and different functions. (biomedcentral.com)
  • The reason we focused on the LGP is that Olig2- and GFAP-astrocytes intermingled and occupied mutually exclusive territories in this nucleus, with possible contrast in function and gene expression. (biomedcentral.com)
  • 21 disease terms (MeSH) has been reported with OLIG2 gene. (cdc.gov)
  • The following product was used in this experiment: OLIG2 Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-85734, RRID AB_2792873. (thermofisher.com)
  • B ) IHC analyses of Olig2, Sox2, and Ki67 in E12.5 Shh- cKO mutant embryo and control littermates (cervical level). (elifesciences.org)
  • No significant difference in the expression of Sox2, Olig2 and Ki67 within the spinal cord. (elifesciences.org)
  • C ) Quantification of the number of LMCm (Isl1 + /FoxP1 + ), LMCl (Hb9 + /FoxP1 + or Lhx1 + /FoxP1 + ), MMC (Hb9 + /Lhx3 + ) and HMC (Hb9 + /Isl1 + ) neurons, Olig2 + , Sox2 + , Ki67 + cells and total MNs at cervical level in E12.5 mouse embryonic spinal cord. (elifesciences.org)
  • These tumors also display EMA staining at the surfaces of ependymal rosettes/canals, as well as in a dot-like or ring-like pattern adjacent to tumor nuclei ( Image D ). Neurofilaments, synaptophysin, OLIG2, SOX10, and NeuN are usually negative. (cap.org)
  • The clear and round cells were positive for S100, synaptophysin and Olig2 (Figure 1k ). (upmc.edu)
  • OLIG2 is universally expressed in glioblastoma and other diffuse gliomas (astrocytomas, oligodendrogliomas and oligoastrocytomas), and is a useful positive diagnostic marker of these brain tumors. (wikipedia.org)
  • Although in normal brain tissue OLIG2 expresses mostly on oligodendrocytes but not on mature astrocytes, in adult glioma, OLIG2 expresses on both IDH1 or IDH2-mutant adult lower grade astrocytoma and oligodendroglioma on similar levels, but it is expressed on a lower level on IDH-wildtype glioblastoma. (wikipedia.org)
  • Here, to uncover the specific functions of Olig2-astrocytes, we first analyzed public single-cell RNA-seq databases of adult mouse brains. (biomedcentral.com)
  • We confirmed that Olig2 and GFAP mRNAs were preferentially expressed in the Olig2- and GFAP-astrocytes, respectively, indicating that the laser microdissection method yielded minimal cross-contamination between two types of cells. (biomedcentral.com)
  • The Olig2-astrocytes expressed significantly higher levels of SLC7A10 mRNA than the GFAP-astrocytes, corroborating the in silico data. (biomedcentral.com)
  • SLC7A10 co-localized with Olig2-genetic labeling, especially on the fine processes of Olig2-astrocytes. (biomedcentral.com)
  • Taken together, our findings suggest that SLC7A10 exerts specific functions in Olig2-astrocytes of the adult brain. (biomedcentral.com)
  • and second, mRNA expression analysis using differentially isolated Olig2- and GFAP-astrocytes from a single section of the LGP by laser microdissection in combination with immunohistochemistry methods. (biomedcentral.com)
  • We identified differentially expressed genes in the Olig2- and GFAP-astrocytes. (biomedcentral.com)
  • Among them, mRNA for SLC7A10, a neutral amino acid transporter, was preferentially expressed in the Olig2-astrocytes. (biomedcentral.com)
  • Our outcomes point out that TFAP2B, ALK and a novel marker OLIG2 might function surrogate markers for PAX3/7-FOXO1 standing what is very helpful in circumstances the place poor high quality tumour tissue will not be appropriate for dependable genetic analyses or reveals inconclusive consequence. (aetox.com)
  • In survival analysis, higher mRNA levels of OLIG2 were associated with better overall survival, but this association was completely dependent on IDH mutation status. (wikipedia.org)
  • This side of anti-neurogenicity of OLIG2 later plays a bigger role in malignancies like glioma. (wikipedia.org)
  • In particular, OLIG2 is selectively expressed in a subgroup of glioma cells that are highly tumorigenic, and is shown to be required for proliferation of human glioma cells implanted in the brain of severe combined immunodeficiency (SCID) mice. (wikipedia.org)
  • Though the molecular mechanism behind this tumorigenesis is not entirely clear, more studies have recently been published pinpointing diverse evidence and potential roles for OLIG2 in glioma progression. (wikipedia.org)
  • It is believed that OLIG2 promotes neural stem cell and progenitor cell proliferation by opposing p53 pathway, which potentially contributes to glioma progression. (wikipedia.org)
  • Olig2-Dependent Reciprocal Shift in PDGF and EGF Receptor Signaling Regulates Tumor Phenotype and Mitotic Growth in Malignant Glioma. (uc.edu)
  • Another phosphorylation site Ser147 predicted by bioinformatics was found to regulate motor neuron development by regulating the binding between OLIG2 and NGN2. (wikipedia.org)
  • Olig2 SUMOylation enhances its genetic targeting ability, which in turn occludes p53 recruitment to promoter for DNA-damage responses. (climat2050.org)
  • 2008 ) Ultraconserved elements in the Olig2 promoter. (neurotree.org)
  • The expression of OLIG2 is mostly restricted in central nervous system, where it acts as both an anti-neurigenic and a neurigenic factor at different stages of development. (wikipedia.org)
  • Most importantly, GFP expression recapitulated endogenous Olig2 expression when R-Olig2 was induced by sonic hedgehog and retinoic acid, and GFP-positive cells could be purified by fluorescence-activated cell sorting. (ca.gov)
  • Instead, the corresponding reductions in RPAIN and Olig2 expression suggest that during the acute phase of withdrawal or abstinence, molecular indices of white matter degeneration may worsen and that additional therapeutic measures may be required for optimum recovery. (ashdin.com)
  • Our evaluation revealed that every one three immunohistochemical markers are related to the presence of PAX3/7-FOXO1 fusion: TFAP2B, OLIG2 and ALK. (aetox.com)
  • Finally, the numbers of OPCs, evaluated with the markers Tcf4 and Olig2, were increased from day 2 (Olig2) or day 7 (Tcf4) post-injury (P ≤ 0.05). (lu.se)
  • OLIG2 has been shown to directly repress the p53 tumor-suppressor pathway effector p21WAF1/CIP1, suppress p53 acetylation and impede the binding of p53 to several enhancer sites. (wikipedia.org)
  • OLIG2 is a novel immunohistochemical marker related to the presence of PAX3/7-FOXO1 translocation in rhabdomyosarcomas. (aetox.com)
  • Instead, it significantly reduced RPAIN (P=0.002) and Olig2 (P=0.02) relative to responses after 8 weeks of continuous ethanol exposure. (ashdin.com)
  • The luciferase plasmid, WT Flag-Olig2, 3KR Flag-Olig2, AQ Flag-Olig2, and/or His-SUMO1 into Neuro-2a cells using Lipofectamine 3000. (climat2050.org)
  • Thus, SUMOylation is important for Olig2 to function as an antiapoptotic factor in genotoxic stress. (climat2050.org)
  • Our work uncovers a SUMOylation-dependent regulatory Rabbit Polyclonal to TUT1 mechanism of Olig2 in regulating cancer survival. (climat2050.org)
  • OLIG2 is well recognized for its importance in cancer research, particularly in brain tumors and leukemia. (wikipedia.org)
  • Olig2-Cre) mutant embryos (n = 4) (lower panel) and control littermates (n = 4) (upper panel). (elifesciences.org)
  • Factor de transcripción de estructura hélice-bucle-hélice básica requerido para la diferenciación de los OLIGODENDROCITOS y las neuronas motoras en la médula espinal y para el desarrollo de las neuronas motoras somáticas en el rombencéfalo. (bvsalud.org)