Odorant binding proteins. Medical search. Frequent questions

Odorant-binding proteins (OBPs) are small (10 to 30 kDa) soluble proteins secreted by auxiliary cells surrounding olfactory receptor neurons, including the nasal mucus of many vertebrate species and in the sensillar lymph of chemosensory sensilla of insects. (wikipedia.org)
OBPs are characterized by a specific protein domain that comprises six α-helices joined by three disulfide bonds. (wikipedia.org)
Although the function of the OBPs as a whole is not well established, it is believed that they act as odorant transporters, delivering the odorant molecules to olfactory receptors in the cell membrane of sensory neurons. (wikipedia.org)
OBPs are small proteins on the order of 14 kDa in size. (wikipedia.org)
OBPs' have a characteristic signature that is recognized by a conserved pattern of six cysteines that are connected in the protein by three disulfide bridges. (wikipedia.org)
They are generally believed to increase the solubility of hydrophobic odorants by binding them and transporting them across the aqueous sensillum lymph to receptors in the dendrites, and several studies support a role for OBPs in olfactory perception in vivo. (wikipedia.org)
Odorant binding proteins (OBPs) are involved in either olfactory or nonolfactory cue sensation depending on their spatial and temporal expression. (bioone.org)
Olfactory sensors based on odor-binding proteins (OBPs) have been utilized to analyze pollutants in food and air samples, while their application for the detection of typical odor-causing compounds in drinking water is rarely reported, partly due to the lack of knowledge about the binding properties of odorants. (springeropen.com)
The predicted amino acid sequences had the highest degree of sequence similarity to the Drosophila melanogaster OBPs named pheromone binding protein-related proteins (PBPRPs). (elsevierpure.com)
Within a sensillum, ORNs are accompanied by several support cells, which secrete odorant binding proteins (OBPs) into the sensillum lymph [ 6 , 7 ]. (ijbs.com)
Insects' odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are essential for the chemical recognition during behavioral responses or other physiological processes. (slu.se)
Odorant binding proteins (OBPs) are small proteins secreted by auxiliary cells surrounding olfactory receptor neurons in the antennae of insects. (frontiersin.org)
then used another molecular technique, fluorescence binding assay, to show that these OBPs, when recombinantly expressed in E. coli bacteria, selectively bind to molecules that are known to be components of the moth's female sex pheromone. (frontiersin.org)

The olfactory receptors of terrestrial animals exist in an aqueous environment, yet detect odorants that are primarily hydrophobic. (wikipedia.org)
The aqueous solubility of hydrophobic odorants is greatly enhanced via odorant-binding proteins, which exist in the extracellular fluid surrounding the odorant receptors. (wikipedia.org)
Some odorant binding proteins are hypothesized to hasten odor response termination by extracting odorant molecules from the sensillar lymph or from receptors themselves. (wikipedia.org)
Schymura D, Forstner M, Schultze A, Kröber T, Swevers L, Iatrou K, Krieger J. Antennal expression pattern of two olfactory receptors and an odorant binding protein implicated in host odor detection by the malaria vector Anopheles gambiae . (ijbs.com)
Odor-detection in the malaria mosquito Anopheles gambiae involves large families of diverse proteins, including multiple odorant binding proteins (AgOBPs) and olfactory receptors (AgORs). (ijbs.com)
The receptors AgOR1 and AgOR2, as well as the binding protein AgOBP1, have been implicated in the recognition of human host odors. (ijbs.com)
members of this family include the calcium-sensing receptor, the GABA(B) receptor, some odorant receptors and some taste receptors. (nih.gov)
Atomic structures of the ligand-binding core of the original metabotropic glutamate receptor 1 obtained using X-ray crystallography provide a foundation for determining the initial receptor activation of this important family of G-protein-coupled receptors. (nih.gov)
In this talk I will review current efforts at computationally characterizing the molecular determinants in the largest membrane-bound receptor family, the G-protein-coupled receptors (GPCRs). (univr.it)
I will focus on odorant receptors, which constitute more than half GPCRs. (univr.it)
Odorant receptors (ORs), the largest subfamily of G proteincoupled receptors, detect odorants in the nose. (bmbreports.org)
Odorant receptors (ORs) are G protein-coupled receptors (GPCRs) that are essential for detecting and distinguishing among odorants. (bmbreports.org)
Olfactory receptors are integral membrane proteins that belong to the seven transmembrane (TM), rhodopsin-like G-protein coupled receptor family. (embl.de)
The main aim of the project is to study the genes that encode the olfactory receptors [ proteins that bind to odorant molecules ]. (fapesp.br)
The results also show that there is a certain amount of redundancy in ligand binding to receptors. (blogspot.com)
Closely related olfactory receptors molecules tend to bind similar odorants. (blogspot.com)
The more kinds of active receptors present in the sensory neurons of the nasal cavity, the greater the capacity to detect low concentrations of odorant. (blogspot.com)

This family is composed of pheromone binding proteins (PBP), which are male-specific and associate with pheromone-sensitive neurons and general-odorant-binding proteins (GOBP). (wikipedia.org)
Obp76a, better known as LUSH, is located trichoid sensilla and is necessary for normal response of the odor receptor Or67d to its pheromone ligand cis-vaccenyl acetate (cVA), although responses of Or67d to cVA have been detected in the absence of Obp76a LUSH has also been found to bind cVA in vitro and is known to bind other insect pheromones, short-chain alcohols, and phthalates. (wikipedia.org)
Predicted to enable pheromone binding activity and small molecule binding activity. (mcw.edu)

Seven genes were identified with sequence similarity to insect odorant-binding protein (OBP) genes. (elsevierpure.com)
Insect OS-D-like proteins, also known as chemosensory (CSP) or sensory appendage proteins (SAP), are broadly expressed in various insect tissues, where they are thought to bind short to medium chain length fatty acids and their derivatives. (gc.ca)
Although their specific function remains uncertain, OS-D-like members have been isolated from sensory organs (including the sensillum lymph in some cases), and a role in olfaction similar to that of the insect odorant binding proteins (OBP) has been suggested for some.We have identified 15 new OS-Dlike sequences: four from cDNA clones described herein and 11 from sequencedatabases. (gc.ca)
and sensory neuron membrane proteins, SNMPs) were identified, and phylogenetic analyses were performed with these genes from Q. mendeli and other model insect species. (bvsalud.org)
Symbiont-induced odorant binding proteins mediate insect host hematopoiesis. (brodericklab.com)

Each chemosensory neuron type in C . elegans expresses multiple chemoreceptors that are likely tuned to different odorants, a subset of which can be behaviorally discriminated [ 27 , 28 ]. (plos.org)

Binding of small molecules to their targets triggers complex pathways. (univr.it)
These conclusions are based on the assumption that each olfactory receptor can bind to a single odorant molecule-or a small number of related molecules. (blogspot.com)

SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)

found that the deletion of the sole abundant OBP, Obp28a, in ab8 sensilla of Drosophila does not reduce the magnitude of their olfactory responses, suggesting that Obp28a is not required for odorant transport and that ab8 sensilla do not require an abundant OBP. (wikipedia.org)
Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster. (expasy.org)

An antennae specific odorant-binding protein is involved in Bactrocera dorsalis olfaction. (mpg.de)
Odorants can also be perceived by entering the nose posteriorly through the nasopharynx to reach the olfactory receptor via retronasal olfaction. (medscape.com)

In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
The valuable information to the binding of OBP2a to typical odorants in this study would provide a theoretical foundation for the development of OBP-based odor detection biosensors to achieve quick detection in drinking water, further helping the improvement of water treatment processes in the water industry. (springeropen.com)
2-Methylisoborneol (2-MIB) and geosmin (GSM), the main earthy/musty odorants in China, have odor thresholds of 10 ng/L and 4 ng/L, respectively [ 10 ]. (springeropen.com)

Insects detect and discriminate volatile odorants by means of olfactory receptor neurons (ORN) located in sensory structures called olfactory sensilla. (ijbs.com)

Altogether, the results demonstrate that both sexes express the two olfactory receptor types as well as the binding protein AgOBP1 but there is a significant sexual dimorphism concerning the number and distribution of these cells. (ijbs.com)

These gene products are here referred to as gustatory PBP-related proteins (GPBPRPs) 1-7. (elsevierpure.com)
In contrast to RNAi, CRISPR-based gene manipulation requires a guide RNA and a programmable Cas endonuclease protein. (technologynetworks.com)
From this cleavage and repair process, sequence insertions or deletions (indels) often cause errors in translation, leading to functional gene, and subsequent protein, knockdown. (technologynetworks.com)
Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions. (antibodiesinc.com)
3] In addition, mutations of the gene encoding chromodomain-helicase DNA-binding protein 7 (CHD7) have been found in some patients with Kallmann syndrome or idiopathic hypogonadotropic hypogonadism. (medscape.com)
In one patient, isolated bioinactive luteinizing hormone (LH) was present as a result of a homozygous mutation in the LH beta subunit gene, which prevented binding of LH to its receptor. (medscape.com)
Homozygous mutations in GPR54, a gene encoding a G protein-coupled receptor, which binds kisspeptin 1, have been reported as a cause of hypogonadotropic hypogonadism. (medscape.com)

This may suggest gender-specific differences in the ability to detect distinct odorants, specifically human host-derived volatiles. (ijbs.com)
Since each receptor is presumably capable of binding a specific odorant, it seems very likely that mice can detect a large number of different smells. (blogspot.com)
2007) decided to test this by surveying 377 individuals for their ability to detect four odorants: isoamyl acetate, isovaleric acid, L-carvone, and cineole. (blogspot.com)
The authors then tried to correlate ability to detect low levels of these odorants with the presence of specific markers for alleles of olfactory receptor genes. (blogspot.com)

As a first step in ultimately combating this bee-barian, researchers in the lab of Leonard Foster at the University of British Columbia in Canada have published a protein atlas in Molecular & Cellular Proteomics that details 1,433 differentially expressed proteins across the various developmental stages of V. destructor. (asbmb.org)

Analysis of food proteins and peptides by mass spectrometry-based techniques. (cnr.it)

A theoretical study on predicted protein targets of apple polyphenols and possible mechanisms of chemoprevention in colorectal cancer. (cnr.it)

A hypothesis on the capacity of plant odorant-binding proteins to bind volatile isoprenoids based on in silico evidences. (cnr.it)

2022) Context-dependent reversal of odorant preference is driven by inversion of the response in a single sensory neuron type. (plos.org)

At some point, we will want to try to define the molecular mechanism that is underlying that interaction, and knowing which proteins are expressed in which stages in the Varroa will be important for that eventual understanding. (asbmb.org)
Our results indicate that context-dependent recruitment of alternative intracellular signaling pathways within a single sensory neuron type conveys opposite hedonic valences, thereby providing a robust mechanism for odorant encoding and discrimination at the periphery. (plos.org)

Giordano D, De Masi L, Argenio MA, Facchiano A. (2021)Structural Dissection of Viral Spike-Protein Binding of SARS-CoV-2 and SARS-CoV-1 to the Human Angiotensin-Converting Enzyme 2 (ACE2) as Cellular Receptor. (cnr.it)

172 Computational analysis and prediction of proteins that undergo domain swapping. (ncbs.res.in)
Computational Approaches for Decoding Select Odorant-Olfactory Receptor Interactions Using Mini-Virtual Screening. (ncbs.res.in)
Computational tools to study RNA-protein complexes. (ncbs.res.in)
Predicting the stability of mutant proteins by computational approaches: an overview. (cnr.it)

In this study, we have explored the expression of these olfactory proteins, as well as the ubiquitous odorant receptor heteromerization partner AgOR7, in the thirteen flagellomeres (segments) of female and male antenna. (ijbs.com)
GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. (antibodiesinc.com)

Bulk Order Inquiry for Anti-Green Fluorescent Protein Antibody ------- (please add any order requirements, including desired quantity, timing, etc. (antibodiesinc.com)

Molecular Docking Simulations on Histone Deacetylases (HDAC)-1 and -2 to Investigate the Flavone Binding. (cnr.it)

In addition, we analyzed the chemical-competitive binding of a widely expressed protein, SfruOBP31, to host plant odorants, sex pheromones, and insecticides. (slu.se)
Further ligands binding assay revealed a broad functional related binding spectrum of SfruOBP31 to host plant odorants, sex pheromones, and insecticides, suggesting its potential function in food, mate seeking, and pesticide resistance. (slu.se)

These proteins were initially identified on the basis of their ability to bind with moderate-affinity radioactively labeled odorants. (wikipedia.org)
Their structures have been investigated to explore new bio-inspired repellents against mosquitoes, with potentially improved OBP binding affinity, selectivity, and reduced volatility. (wikipedia.org)
The binding affinity of OBP2a to fatty aldehydes including hexanal, heptanal, 2-octenal, decanal, and β-cyclocitral increased with the increase of hydrophobicity of aldehydes. (springeropen.com)
DEELIG: A Deep Learning Approach to Predict Protein-Ligand Binding Affinity. (ncbs.res.in)

Active enhancers strengthen insulation by RNA-mediated CTCF binding at chromatin domain boundaries. (ncbs.res.in)
In addition to quantifying the mites' proteins, the researchers found that males and females expressed proteins involved in chromatin remodeling differently. (asbmb.org)

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. (antibodiesinc.com)
Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund's adjuvant. (antibodiesinc.com)

Bioinformatics analysis of mutations sheds light on the evolution of Dengue NS1 protein with implications in the identification of potential functional and druggable sites. (ncbs.res.in)

We show that these opposing responses are driven via engagement of distinct odorant-directed signal transduction pathways within AWC. (plos.org)

Predicted to act upstream of or within G protein-coupled receptor signaling pathway and sensory perception of smell. (jax.org)

Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)
Collation and analyses of DNA-binding protein domain families from sequence and structural databanks. (ncbs.res.in)
A neighbor joining distance phenogram identified several protein similarity classes that were characterized by highly conserved sequence motifs, including (A) N-terminal YTTKYDN(V/I)(N/D)(L/V)DEIL, (B) central DGKELKXX(I/L)PDAL, and (C) C-terminal KYDP. (gc.ca)

According to Foster, Varroa's use of odorant-binding proteins is likely key to their predatory interactions. (asbmb.org)

Part of what that might be pointing to is the mites exposing different parts of the DNA to express more sex-specific proteins. (asbmb.org)
Biochemical characterization and homology modeling of a purine-specific ribonucleoside hydrolase from the archaeon Sulfolobus solfataricus: Insights into mechanisms of protein stabilization. (cnr.it)
Olfactory transduction is believed to be initiated by the binding of odorants to specific receptor proteins in the cilia of olfactory receptor cells. (embl.de)

Koganezawa, M & Shimada, I 2002, ' Novel odorant-binding proteins expressed in the taste tissue of the fly ', Chemical Senses , vol. 27, no. 4, pp. 319-332. (elsevierpure.com)

2016. An Approach to Function Annotation for Proteins of Unknown Function (PUFs) in the Transcriptome of Indian Mulberry. . (ncbs.res.in)

They are incapable of producing a full-length functional receptor protein. (blogspot.com)

A census of actin-associated proteins in humans. (ncbs.res.in)

Performance of Web tools for predicting changes in protein stability caused by mutations. (cnr.it)

Bioinformatics comparisons of RNA-binding proteins of pathogenic and non-pathogenic Escherichia coli strains reveal novel virulence factors. (ncbs.res.in)

Anatomy6

Organisms1

Chemicals and Drugs6

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Psychiatry and Psychology2

Phenomena and Processes4

Information Science3

Health Care1

OBPs13

Receptors17

Pheromone3

Insect5

Chemosensory1

Molecules2

Structural Classifi2

Drosophila2

Olfaction2

Odor3

Insects1

Sexual dimorphism1

Gene7

Detect4

Developmental stages1

Peptides1

Targets1

Volatile1

20221

Mechanism2

20211

Computational4

Ubiquitous2

Antibody1

Molecular1

Pheromones2

Affinity4

Chromatin2

Fluorescent2

Identification1

Distinct1

Receptor signali1

Sequence3

Interactions1

Specific3

Chemical1

Transcriptome1

Functional1

Humans1

Mutations1

Coli1