Oct4 nanog. Medical search. Frequent questions

genes: Nanog, Oct4 and Sox2 (Figure 1 ). (lu.se)
To understand the relationship between motif syntax and transcription factor binding, we train a deep learning model that uses DNA sequence to predict base-resolution binding profiles of four pluripotency transcription factors Oct4, Sox2, Nanog, and Klf4. (nih.gov)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)
Life Biosciences, a startup co-founded by David Sinclair, is exploring the regenerative capacity of three Yamanaka factors (Oct4, Sox2 and Klf4). (scientificamerican.com)
Enhances the reprogramming efficiency of mouse embryonic fibroblasts transfected with OCT4, SOX2, KLF4, and c-MYC (Li et al. (stemcell.com)
In this study, Jean-Marc Lemaitre and his team firstly confirmed that this was not possible using the batch of four genetic factors (OCT4, SOX2, C MYC and KLF4) traditionally used. (eurekalert.org)
For instance, OCT4, SOX2, and NANOG are the transcription factors analyzed in most studies. (atlasantibodies.com)
Conversely, REAC treatment elicited a biphasic effect on a number of stemness-related genes, leading to early transcriptional increase of Oct4, Sox2, cMyc, Nanog, and Klf4 within 6-20 h, followed by a downregulation at later times. (nih.gov)
The reporter responds to the activity of stemness master transcriptional factors SOX2 and OCT4 through six concatenated repeats of a SOX2/OCT4 response element (SORE6) [ 14 ]. (springer.com)
Calpain-6 expression depended on the stem-cell transcription network that involves Oct4, Nanog, and Sox2 and was activated by hypoxia. (hal.science)
L'expression de la calpaïne-6 dépend d'un programme génique de cellules souche qui implique Oct4, Nanog et Sox2 et est activée par l'hypoxie. (hal.science)
Further, treatment of colon cancer or hepatoblastoma cells grown under cancer stem cell conditions reduced sphere formation in number and size as well as expression of the stemness markers SOX2, NANOG, and OCT4. (uni-muenchen.de)
enhances iPSC reprogramming four-fold and accelerates the appearance of iPSC colonies in combination with 4F (Oct4, Sox2, c-Myc and Klf4). (tocris.com)
With the exception of Myc (which was only present in a subset of benign, borderline, and malignant tumors), Oct4, Nanog, Sox2, and Klf4 were detectable at variable levels across both normal and diseased tissues. (medscape.com)
Semi-quantitative evaluation of our immunohistochemical staining revealed that protein expression of Oct4, Nanog, Myc, and Sox2, but not Klf4, was significantly increased in benign, borderline, and malignant vascular tumors relative to non-diseased vascular tissue controls. (medscape.com)

NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency. (nih.gov)
Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. (mendeley.com)
The Human Nanog pGreenZeo Differentiation Reporter co-expresses dscGFP (destabilized copGFP, 2-hour half-life) and zeomycin resistance from the human Nanog promoter/enhancer elements, enabling tracking of pluripotency/differentiation using GFP fluorescence and selection for the desired cells using zeomycin. (reportergene.com)
Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. (bdbiosciences.com)
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. (bdbiosciences.com)
Nanog and transcriptional networks in embryonic stem cell pluripotency. (bdbiosciences.com)
Our data demonstrated that EGCG inhibited the expression of pluripotency maintaining transcription factors (Nanog, c-Myc and Oct-4) and self-renewal capacity of pancreatic CSCs. (weeksmd.com)
The team subsequently focused on the examination of effects of centrosome depletion on stem cell properties to find that the centrosome loss led to downregulation of regulators of pluripotency OCT-4 and NANOG and a concomitant increase in the expression of proteins which marked the initiation of differentiation program (namely p53, PAX-6, brachyury etc. (databasefootball.com)

Here are their technical properties: AECs have multipotent differentiation potential and expression of pluripotent stem cell specific transcription factors including Oct4 and Nanog. (parentsguidecordblood.org)
The genetic regulatory network consisting of the Oct4, Nanog, and TET transcription factors (TF's) is understood to control cell fate, specifically the transitions between somatic and pluripotent states. (aps.org)
We conclude that the effective rate of DNA methylation increases stability (relative size of the basin of attraction) of the somatic cell state, while increasing DNA de-methylation rate, which is controlled by Nanog-guided TET protein, increases stability of the pluripotent state. (aps.org)
whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)

Inhibition of Nanog by shRNA enhanced the inhibitory effects of EGCG on self-renewal capacity of CSCs. (weeksmd.com)

HiPSC express pluriotency markers OCT4, Nanog, LIN28 and SSEA-4. (cellapplications.com)

They then added two additional factors (NANOG and LIN28) that made it possible to overcome this barrier. (eurekalert.org)

By crossreferencing methylation patterns to genome-wide mapping of histone H3 lysine (K) 4/27 trimethylation and binding of Oct4, Nanog, and Polycomb proteins on gene promoters, we found that promoter DNA methylation is the only marker of this group present on approximately 30% of genes, many of which are silenced in mES cells. (ca.gov)
In demethylated mutant mES cells, we saw upregulation of a subset of X-linked genes and developmental genes that are methylated in wild-type mES cells, but lack either H3K4 and H3K27 trimethylation or association with Polycomb, Oct4, or Nanog. (ca.gov)
Novel Roles of Nanog in Cancer Cells and Their Extracellular Vesicles. (nih.gov)
NANOG regulates epithelial-mesenchymal transition via AMPK/mTOR signalling pathway in ovarian cancer SKOV-3 and A2780 cells. (nih.gov)
NANOG confers resistance to complement-dependent cytotoxicity in immune-edited tumor cells through up-regulating CD59. (nih.gov)
Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. (stemcell.com)
ERK-Mediated Loss of miR-199a-3p and Induction of EGR1 Act as a "Toggle Switch" of GBM Cell Dedifferentiation into NANOG- and OCT4-Positive Cells. (harvard.edu)

OCT4/ Nanog protein expression also correlated positively with fibrosis stage in liver biopsies from patients with chronic HBV or HCV infection . (bvsalud.org)

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-ß1-Induced OCT4/Nanog Pathway. (bvsalud.org)
In conclusion, HBV and HCV independently and cooperatively promote liver fibrogenesis through a TGF-ß1-induced OCT4/Nanog-dependent pathway. (bvsalud.org)

We used CRISPR -Cas9 to knock out OCT4 and Nanog to evaluate their effects on HBV-, HCV-, or TGF-ß1-induced liver fibrogenesis. (bvsalud.org)

Nanog, as a key cancer stem cell marker in tumor progression. (nih.gov)

Promoter CpG methylation contributes to ES cell gene regulation in parallel with Oct4/Nanog, PcG complex, and histone H3 K4/K27 trimethylation. (ca.gov)

OCT4 and Nanog guide RNA independently suppressed HBV-, HCV-, HBV/HCV-, and TGF-ß1-induced α-SMA, TIMP-1 , and Col1A1 expression and reduced Huh7.5.1, LX2, primary hepatocyte , and primary human HSC migratory capacity. (bvsalud.org)
Of these subpopulations, the adherent AECs have the lowest expression of Oct4 and Nanog. (parentsguidecordblood.org)

Octamer binding transcription factor 4 (OCT4) and Nanog are direct targets of the profibrogenic TGF-ß1 signaling cascade. (bvsalud.org)

While active enhancers associate with TFs, only a minority of regions marked by NANOG, OCT4, H3K27ac and H3K4me1 function as enhancers, with activity changing markedly with culture conditions. (biorxiv.org)

The data on expression and clinical impact of cancer stem cell markers SOX2, NANOG and OCT4 in lung cancer is still lacking. (sagepub.com)
The aim of our study was to compare SOX2, NANOG and OCT4 mRNA expression levels in whole blood between advanced small-cell lung cancer (SCLC) patients and healthy controls, and to correlate mRNA expression with progression-free survival (PFS) after first-line chemotherapy and overall survival (OS) in advanced SCLC patients. (sagepub.com)
SOX2, NANOG and OCT4 mRNA expression levels were determined using TaqMan qPCR in whole blood collected prior to chemotherapy. (sagepub.com)
2. Embryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma. (nih.gov)
3. Transcriptional regulation of nanog by OCT4 and SOX2. (nih.gov)
4. [LincRNA-ROR functions as a ceRNA to regulate Oct4, Sox2, and Nanog expression by sponging miR-145 and its effect on biologic characteristics of colonic cancer stem cells]. (nih.gov)
8. Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4. (nih.gov)
12. SUMOylation represses Nanog expression via modulating transcription factors Oct4 and Sox2. (nih.gov)
These outcomes were accompanied by higher expressions of self-renewal genes or proteins or both, including OCT4, SOX2, NANOG, and cytokine receptor IL6ST, as well as pluripotency and the cell fate regulator cyclin D1. (nih.gov)
SOX2, NANOG and OCT4) and by extrinsic pathways (i.e. (aging-us.com)
Adult somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) with the overexpression of key reprogramming genes (OCT4, KLF4, SOX2, cMYC, NANOG and LIN28). (sigmaaldrich.com)
In this study, Jean-Marc Lemaitre and his team firstly confirmed that this was not possible using the batch of four genetic factors (OCT4, SOX2, C MYC and KLF4) traditionally used. (eurekalert.org)
2. Falsified one (1) figure for the real-time RT-PCR data for endogenous SOX2 expression in human iPSCs derived from dermal (HiPS-E1) and cardiac (HiPS-E2) fibroblasts and iPSCs generated from peripheral blood mononuclear cells derived from coronary artery disease patients (HiPS-ECP1, HiPS-ECP2, and HiPS-ECP3) by substituting real-time RT-PCR data for endogenous OCT4 expression in the forementioned cell lines. (nih.gov)
After passaged, a subpopulation of these cells showed low expression of SSEA-4 but were negative for other important ESC surface markers such as Tra-1-60, Tra-1-81, and transcription factors like octamer-binding transcription factor 4 (Oct4), SRY(sex determining region Y)-box 2 (Sox2), and Nanog. (molvis.org)
1999). POU5F1 (OCT4), SOX2, and NANOG bind the promoter of the FGF2 gene and POU5F1 (Babaie et al. (reactome.org)
Here we show that a combination of modified reprogramming factors (OySyNyK), in which the transactivation domain of the Yes-associated protein is fused to OCT4, SOX2 and NANOG respectively, could be used to establish a highly efficient and rapid reprogramming system for iPSC generation. (nih.gov)
Expression of the pluripotent markers Sox2 and Oct4 , and the primitive germ cell markers Blimp1 and Stella were not detected. (oncotarget.com)
G354Organism:HumanVector:Refer to Individual Virus PagesPromoter:PGKGene:Oct4, Sox2, Nanog, Lin28 and GFP control virus.Accession Number:Q01860, P48431, Q9H9S0 and Q9H9Z2Titer:1x10^7 IU/mlShipping. (topsan.org)
G351Organism:HumanVector:pLenti-III-EF1α-Polytronic-OSNLGPromoter:EF1αGene:Oct4, Sox2, Nanog, Lin28 and GFPAccession Number:Q01860, P48431, Q9H9S0 and Q9H9Z2Titer:1x10^7 IU/mlShipping Condition:Dry. (topsan.org)
Our analyses reveal the ESC TRN is rich in feedback, with global feedback structure critically dependent on Nanog, Oct4 and Sox2, which collectively participate in over two thirds of all feedback loops. (soton.ac.uk)
Significant association of combination of OCT4, NANOG, and SOX2 gene polymorphisms in susceptibility and response to treatment in North Indian breast cancer patients. (cdc.gov)
The overexpressed SOX2 enhances the expression of key pluripotent factors (OCT4 and NANOG) and suppresses differentiation lineage factors (HOPX and NKX2-1), driving cancer cells toward a stem-like state. (nih.gov)
Recent ChIP experiments of human and mouse embryonic stem cells have elucidated the architecture of the transcriptional regulatory circuitry responsible for cell determination, which involves the transcription factors OCT4, SOX2, and NANOG. (nih.gov)
A search for the OCT4-SOX2-NANOG network motif in other species reveals that it is unique to mammals. (nih.gov)
With a kinetic modeling approach, we ascribe function to the observed OCT4-SOX2-NANOG network by making plausible assumptions about the interactions between the transcription factors at the gene promoter binding sites and RNA polymerase (RNAP), at each of the three genes as well as at the target genes. (nih.gov)
The switch stabilizes the expression levels of the three genes, and through their regulatory roles on the downstream target genes, leads to a binary decision: when OCT4, SOX2, and NANOG are expressed and the switch is on, the self-renewal genes are on and the differentiation genes are off. (nih.gov)
Increasing the binding strength of NANOG to OCT4 and SOX2, or increasing its basal transcriptional rate, leads to an irreversible bistable switch: the switch remains on even when the activating signal is removed. (nih.gov)
We also suggest tests that could discriminate between a variety of feedforward regulation architectures of the target genes by OCT4, SOX2, and NANOG. (nih.gov)

In these multinucleate cells, activation of human pluripotency genes such as Oct4 and Nanog occurs rapidly (24hrs) and efficiently (70% of single heterokaryons). (ca.gov)
We hypothesize that expression of pluripotency genes (Nanog, oct4, Llf4, etc.) continue to be expressed in the entire ectoderm after gastrulation, which ensures a gradual ectodermal patterning process to the different domains. (nih.gov)
Depending on the cellular context, AHR silences the expression of pluripotency genes Oct4 and Nanog and potentiates differentiation, whereas curtailing cellular plasticity and stemness. (nih.gov)

Transcriptional inhibition of OCT4 and Nanog via inhibiting GLI1 was important for penfluridol-induced stemness and proliferation inhibition. (nature.com)
6. Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression. (nih.gov)
13. MicroRNA 630 Represses NANOG Expression through Transcriptional and Post-Transcriptional Regulation in Human Embryonal Carcinoma Cells. (nih.gov)
We found a specific interaction of DNMTs during early differentiation with the PAF1 (polymerase-associated factor 1) transcriptional elongation complex, which binds to promoters of the pluripotency and known DNMT target genes encoding OCT4 and NANOG, thereby providing a possible molecular link for the silencing of these genes during differentiation. (nih.gov)
In the first part of the thesis we investigate the possibility that fluctuations in the transcription factor Nanog { which is central to the embryonic stem cell transcriptional regulatory network (ESCTRN) { regulate population variability by controlling important feedback mechanisms. (soton.ac.uk)
We develop chemical master equation, chemical Langevin equation and reaction rate equation models of the reporter system to determine how this might disturb normal Nanog transcriptional control. (soton.ac.uk)

Octamer‑binding transcription factor 4 (OCT4) and circulating tumor cells (CTCs) are key factors associated with tumor metastasis and drug resistance in cancer. (spandidos-publications.com)
Octamer-binding transcription factor 4 (OCT4), located on chromosome 6p21 in the human genome, is a stem cell marker that serves a crucial role in the carcinogenesis of several types of cancer, including pancreatic cancer, ovarian cancer and breast cancer ( 17 - 20 ). (spandidos-publications.com)

These analyses indicate that Nanog fluctuations regulate population heterogeneity by transiently activating different regulatory subnetworks, driving transitions between a Nanog-expressing, feedback-rich, robust and self-perpetuating pluripotent state and a Nanog-diminished, feedback-sparse and differentiation-sensitive state. (soton.ac.uk)

17. Fibroblast growth factor receptor 2 homodimerization rapidly reduces transcription of the pluripotency gene Nanog without dissociation of activating transcription factors. (nih.gov)
The genetic regulatory network consisting of the Oct4, Nanog, and TET transcription factors (TF's) is understood to control cell fate, specifically the transitions between somatic and pluripotent states. (aps.org)

Previous studies demonstrated the prognostic value of OCT4-positive (OCT4 + ) CTCs in patients with cancer ( 16 , 21 ). (spandidos-publications.com)

18. Coexpression of gene Oct4 and Nanog initiates stem cell characteristics in hepatocellular carcinoma and promotes epithelial-mesenchymal transition through activation of Stat3/Snail signaling. (nih.gov)
Resveratrol inhibits pluripotency maintaining factors (Nanog, Sox-2, c-Myc and Oct-4) and drug resistance gene ABCG2 in CSCs. (greenmedinfo.com)
Exposure to VPA resulted in distorted marker gene expression, characterized by a relative increase in NANOG and OCT4 and a reduction in PAX6. (uni-koeln.de)

During DNA damage, p53 is activated (via Ser315 phosphorylation) and binds the Nanog promoter to repress its expression. (aging-us.com)

We conclude that the effective rate of DNA methylation increases stability (relative size of the basin of attraction) of the somatic cell state, while increasing DNA de-methylation rate, which is controlled by Nanog-guided TET protein, increases stability of the pluripotent state. (aps.org)

Summarizing, DRD2 antagonists such as penfluridol induce UPR signaling and suppress the GLI1/OCT4/Nanog axis in ccRCC cells to reduce their growth through inducing autophagy-mediated apoptosis and stemness inhibition. (nature.com)
Inhibition of Nanog by shRNA enhances the inhibitory effects of resveratrol on self-renewal capacity of CSCs. (greenmedinfo.com)

Human pancreatic CSCs expressing high levels of CD133, CD24, CD44, ESA, and aldehyde dehydrogenase also express significantly more Nanog, Oct-4, Notch1, MDR1 and ABCG2 than normal pancreatic tissues and primary pancreatic cancer cells. (greenmedinfo.com)

Oct4 expressed in 100% of tumor tissue samples. (acbjournal.org)

However, recent evidence suggests that these reporters may not give a faithful reflection of endogenous Nanog expression because the introduction of the reporter construct can perturb the kinetics of the underlying regulatory network. (soton.ac.uk)
Our analyses indicate that the reporter construct can weaken the strength of autoactivatory feedback loops that are central to Nanog regulation, and thereby qualitatively perturbs endogenous Nanog dynamics. (soton.ac.uk)

In clinical ccRCC specimens, positive correlations of DRD2 with GLI1, OCT4, and Nanog were observed and their expressions were correlated with worse prognoses. (nature.com)

Similarly, CSCs from Kras(G12D) mice express significantly higher levels of Nanog and Oct-4 than pancreatic tissues from Pdx-Cre mice. (greenmedinfo.com)

The present prospective study aimed to investigate the prevalence of OCT4‑positive (OCT4 + ) CTCs and the potential association with the clinical features and survival of patients with metastatic castration‑resistant prostate cancer (mCRPC) treated with abiraterone + prednisone. (spandidos-publications.com)
Notably, results of a previous study demonstrated that OCT4 + CTCs are associated with advanced stage and distant metastasis in patients with non-small-cell lung cancer ( 21 ). (spandidos-publications.com)
Systematic expression alteration analysis of master reprogramming factor OCT4 and its three pseudogenes in human cancer and their prognostic outcomes. (cdc.gov)

To investigate the role of Nanog further we therefore sought to model in detail the dynamics of Nanog expression in heterozygous fluorescent knock-in reporter cell lines. (soton.ac.uk)

No significant differences were observed for NANOG or OCT4 expression levels when comparing SCLC patients and healthy controls neither when analysing survival outcomes in SCLC patients. (sagepub.com)

Notably, CTC + /OCT4 + occurrence was associated with visceral metastasis and high levels of LDH. (spandidos-publications.com)
In conclusion, OCT4 + CTCs were highly prevalent in patients with mCRPC and associated with visceral metastasis and increased levels of LDH. (spandidos-publications.com)

These results question the efficacy of commonly used reporter strategies and therefore have important implications for the design and use of synthetic reporters in general, not just for Nanog. (soton.ac.uk)

Thus, the presence of OCT4 + CTCs may serve as an independent prognostic factor for patients with mCRPC treated with abiraterone + prednisone. (spandidos-publications.com)

P=0.016) in CTC + /OCT4 + compared with CTC + /OCT4 ‑ and CTC ‑ patients. (spandidos-publications.com)

The majority of studies characterising heterogeneity in Nanog expression have used live-cell fluorescent reporter strategies. (soton.ac.uk)

Anatomy48

Organisms12

Diseases23

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Psychiatry and Psychology2

Phenomena and Processes59

Disciplines and Occupations3

Information Science6

Health Care2

Sox216

Pluripotency8

Pluripotent4

CSCs1

Markers1

LIN281

Cells7

Protein1

Pathway2

CRISPR1

Stem cell1

Promoter1

Expression2

Direct1

Activity1

SOX228

Pluripotency genes3

Transcriptional6

Octamer-binding transcriptio2

Differentiation1

Transcription factors2

Prognostic value1

Gene3

Repress1

Pluripotent state1

Inhibition2

CD441

Tumor1

Endogenous2

Correlations1

Tissues1

Cancer3

Investigate1

Significant1

Levels2

Results1

Factor1

Patients1

Studies1