AnatomyDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
MyometriumUterine ContractionLabor, ObstetricPregnancyOxytocicsRioprostilOxytocinLabor OnsetReceptors, OxytocinLeiomyomaUterusParturitionTocolytic AgentsUterine NeoplasmsObstetric Labor, PrematureAdrenergic beta-3 Receptor AgonistsCesarean SectionHydantoinsReceptors, ProstaglandinPregnancy Trimester, ThirdRNA, MessengerTissue Culture TechniquesReverse Transcriptase Polymerase Chain ReactionReceptors, Corticotropin-Releasing HormoneMuscle RelaxationBlotting, WesternEthersEncyclopedias as TopicHydroxyprogesteronesSilanesKetonesCyclizationStereoisomerismPostpartum HemorrhageTrophoblastic Tumor, Placental SiteUterine InertiaTrophoblastic NeoplasmsLabor Stage, Third
VitroUterusSpontaneousMRNALabourCervixTissueCompoundsEmbryonicHysterectomyContractilityMyometrial cellsDeciduaContractionPregnantPregnancyGenesSamplesOptimalStimulationSuspensionMyocardiumExpressionConcentrationCellFetalInterfereReceptorsResultsBiologicalMaternalParametersFunctionInvestigateNormalKeywordsAortaStudyMiddleLevelDetermine
Vitro4
  • This study, using in vitro strips of human uterine smooth muscle, aimed to investigate the direct non-genomic effects of 17P on spontaneous and induced contractions in tissues obtained during pregnancy, and in the non-pregnant state. (biomedcentral.com)
  • In vitro Studies of Protein Transfer Across Human Fetal Membranes. (researchgate.net)
  • Aim - to reveal and compare groups of genes characterized by increased and reduced expression upon combined stimulation of human macrophages with NOD1 and TLR4 agonists in vitro . (immunologiya-journal.ru)
  • 2001): Melatonin inhibits spontaneous and oxytocin-induced contractions of rat myometrium in vitro. (ankara.edu.tr)
Uterus6
  • Although the existing models of the myometrium and the gravid human uterus are based on the accurate application of general principles of solid mechanics and incorporate some morphological data on the structure and function of the organ, these models remain of limited biomedical value. (witpress.com)
  • To further elucidate the mechanisms regulating PR isoform expression in the human uterus at labour, we have (i) determined the methylation profile of the cytosine-guanine dinucleotides (CpG) island in the promoter region of the PR gene and (ii) identified the histone-modifying enzymes that target the H3K4me3 mark at the PR promoters in term and preterm human myometrial tissues obtained before and after labour onset. (edu.au)
  • The human placenta is in the mature state, an about 500 to 600 grams and heavy to 20 centimeter in diameter 15 organ after implantation ( nidation ) of the blastocyst forms in the uterus. (zxc.wiki)
  • Subsequently, from the obtained uterus samples, determination of the levels of asymmetrical dimethyl arginin (ADMA), symmetrical dimethyl arginin (SDMA), and arginine was made using the high-performance liquid chromatography (HPLC) and levels of Heat shock protein 90 (Hsp90), TLR4 (Toll Like Receptor 4) and NFκB (Nuclear factor kappa-light-chain-enhancer of activated B cells) were measured using the Western blot technique. (ankara.edu.tr)
  • Using microscopes to examine tissue samples taken from patients' bodies, Rokitansky found endometrial cells growing in unusual parts of the body, such as the myometrium, the middle tissue layer of the uterus , and the ovaries. (asu.edu)
  • Public health efforts to help women achieve and maintain a healthy weight and obtain sufficient physical activity can reduce the risk for developing cancer of the endometrium (the lining of the uterus), the most common uterine cancer. (cdc.gov)
Spontaneous2
  • Results of numerical simulations obtained for different values of parameters and constants resembled patterns of spontaneous contractility observed during normal labor and reproduced voltageclamp traces recorded experimentally on pregnant rats and human non-pregnant myometrial cells. (witpress.com)
  • The aims of this study were focused specifically on the latter mechanism i.e. to investigate the direct effects of 17P on contractions of isolated human myometrium, both spontaneous and agonist-induced, in tissue obtained during pregnancy and in the non-pregnant state. (biomedcentral.com)
MRNA11
  • 3 Initial studies of animals not in labour using cDNA probes demonstrated transcripts of similar size to the human genes for prostanoid EP2, EP3, EP4, and FP receptor mRNA using Northern blot in myometrium. (gla.ac.uk)
  • Myometrium from the lower uterine segment (LUS) had greater expression of EP2 receptor mRNA and less expression of EP3 mRNA compared with the fundus and corpus. (gla.ac.uk)
  • EP2 mRNA was most abundant in cervix, EP3 was most abimdant in myometrium and EP4 mRNA was most abundant in decidua. (gla.ac.uk)
  • 4 When expression of prostanoid receptor genes was studied in myometrium obtained from animals both in labour and not in labour and the techniques employed were optimized (principally the use of riboprobes), transcripts of similar size to the human genes were detected for prostanoid EP1, EP2, EP3, EP4, IP, FP and TP receptor mRNA using Northern blot. (gla.ac.uk)
  • Expression of EP1, EP3 and IP receptor mRNA was significantly higher in myometrium from the fundus (compared with lower segment) whereas EP2 gene expression was significantly lower in the fundus. (gla.ac.uk)
  • However, the propensity of some MAPCAs to develop stenosis rather than growth may impair the response to reconstructions.Probe sets prepared from MAPCAs, PA, and aorta mRNA were used to interrogate human genome microarrays. (stanford.edu)
  • Moreover, Placental Gal4 mRNA is increased in placental culture HPL during human pregnancy. (attemptmyexam.com)
  • Receptor densities ended up very similar for human myocardium from all regions (P,.05, ANOVA), while larger stages ended up noticed in aorta (Desk 1).Detection of mRNA for kisspeptin receptor in human cardiomyocytes. (gpr44.com)
  • A) Expression of kisspeptin receptor mRNA in samples (n = 3) of human myometrium (Lanes 1), applied as a manage to verify the specificity of PCR primers as this is a tissue in which the receptor protein has been earlier determined. (gpr44.com)
  • PCR product sizing was established making use of a a hundred bp DNA ladder (Lane 4) (B) Expression of kisspeptin receptor mRNA (predicted sizing 198 bp) in cDNA from human isolated cardiomyocyte samples (n = three, lanes 3). (gpr44.com)
  • Here, we used antigenic cartography to analyze patterns of cross-reactivity among 21 variants and 15 groups of human sera obtained after primary infection with 10 different variants or after messenger RNA (mRNA)-1273 or mRNA-1273.351 vaccination. (cdc.gov)
Labour6
  • 5 When expression of prostanoid receptor genes was studied in cervix obtained from animals both in labour and not in labour, clear signals which were similar in estimated size to the human genes were detected by Northern analysis for EP1, EP2, EP3, EP4, FP, IP and TP receptors. (gla.ac.uk)
  • 6 When expression of prostanoid receptor genes was studied in decidua and chorion obtained from animals both in labour and not in labour, expression of the genes encoding the EP1 and FP receptor in decidua and the EP4 receptor in chorion was lower with advancing gestational age. (gla.ac.uk)
  • Conclusions This study provides further evidence of non-infection related inflammatory pathways contributing to term labour, in particular identifying inflammatory changes in the decidua similar to those in myometrium and cervix. (bmj.com)
  • Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expression is altered to favour PR-A, which activates pro-labour genes. (edu.au)
  • Our studies suggest that epigenetic changes mediated by JARID1A, SMYD3 and DNA methylation may be responsible, at least in part, for the functional progesterone withdrawal that precipitates human labour. (edu.au)
  • To explore the mechanisms behind Pyl A-augmented LPS-induced preterm labour, key mediators of inflammation in the myometrium were investigated. (mdmsignaling.com)
Cervix1
  • Samples of myometrium (from multiple uterine sites), cervix, decidua and chorion were obtained. (gla.ac.uk)
Tissue4
  • Ethical committee approval for tissue collection was obtained from the Research Ethics Committee at University College Hospital Galway and recruitment was by written informed consent. (biomedcentral.com)
  • Cells were isolated from myometrial tissue obtained from women undergoing hysterectomy, and retroviral infection was used to express the catalytic subunit of telomerase in myometrial cells. (elsevierpure.com)
  • In tissue from all a few species, bands ended up attenuated by pre-absorption of the key antibody with the appropriate immunizing peptide.By receptor autoradiography certain binding of [125I]KP-fourteen was noticed in all locations of human and rat myocardium. (gpr44.com)
  • The human MSC derived from bone marrow, adipose tissue, and umbilical cord matrix are from different origins, but with comparable biological properties and function. (promocell.com)
Compounds4
  • The model reproduces electromechanical wave phenomena in the myometrium under normal physiological conditions and after application of different classes of pharmacologically active compounds. (witpress.com)
  • It will be studied the effect of intracellular administration of the following compounds on G36-induced intracellular calcium mobilization, if any significant effect will be obtained with G36 alone: - ryanodine - ryanodine-sensitive calcium channels inhibitor, - xestospongine C - IP3-sensitive calcium channels inhibitor, - bafilomicyn - lisosomal calcium stores destroyer, d. (umfcv.ro)
  • c. effect of several compounds - which interfere with calcium channels - on GPER stimulation with G36, on uterine strips, if it will be obtained any effect with G36 alone: - nifedipine - membranal calcium channels type L inhibitor, - ryanodine - ryanodine-sensitive calcium channels inhibitor, - diethyl phenyl amino borate - IP3-sensitive calcium channels inhibitor, - bafilomicyn - lisosomal calcium stores destroyer. (umfcv.ro)
  • Compounds obtained by chemical synthesis that are analogs or derivatives of naturally occurring prostaglandins and that have similar activity. (lookformedical.com)
Embryonic1
  • showed, for the first time, that RHAMM is differentially expressed during all stages of preimplantation human embryos and human embryonic stem cells (hESC), and indicated that RHAMM knockdown results in down-regulation of several pluripotency markers in hESCs, induction of early extraembryonic lineage, loss of cell viability, and changes in hESC cycle (2). (jri.ir)
Hysterectomy2
  • It will be used non-pregnant myometrium preleaved during myomectomy or hysterectomy for uterine fibromatosis and pregnant myometrium fragments obtained during cesarean operation or needed hysterectomy during delivery. (umfcv.ro)
  • Biopsies of human myometrium were obtained at elective cesarean section, and from hysterectomy specimens, and dissected strips suspended for isometric recordings. (biomedcentral.com)
Contractility1
  • There was no significant direct effect exerted by 17P on pregnant or non-pregnant human myometrial contractility. (biomedcentral.com)
Myometrial cells1
  • Several strategies have been described for the primary culture of human myometrial cells. (elsevierpure.com)
Decidua1
  • The last compartment, the non-decidua compartment, is a layer of nontransformed fibroblasts situated close to the myometrium. (jri.ir)
Contraction3
  • PGE2 induced relaxation of the circular muscle and contraction of the longitudinal muscle has been reported in human, guinea-pig and rat small intestine [15] as well as in human colon [16]. (fxr-agonists.com)
  • The contraction-relaxation responses of the myometrium were also determined in the organ baths. (ankara.edu.tr)
  • Drugs that stimulate contraction of the myometrium. (lookformedical.com)
Pregnant3
  • pregnant and non-pregnant myometrium. (umfcv.ro)
  • Experiments will be performed on non-pregnant and pregnant myometrium preleaved from female rats. (umfcv.ro)
  • Finding the best protocols for obtaining cell cultures from rat myometrium smooth muscle, from both diestrum and 3 weeks pregnant female rats. (umfcv.ro)
Pregnancy7
  • What is the role of human placental lactogen (hPL) in metabolic adjustments during pregnancy? (attemptmyexam.com)
  • Home - Can Someone Do Anatomy and Physiology Exam - What is the role of human placental lactogen (hPL) in metabolic adjustments during pregnancy? (attemptmyexam.com)
  • however, this difference has neverWhat is the role of human placental lactogen (hPL) in find more information adjustments during pregnancy? (attemptmyexam.com)
  • It is not clear whether HPL is required in pregnancy because there are so many differences in many proteins (mostly albumin or albumins) between fetus and placental culture medium known as amniotic fluid, such as lutein and amyl recommend to clarify the role of human placental lactogen in glucose metabolism. (attemptmyexam.com)
  • Thus, placentation and early study of HPL gene expression may affect Placental Glucose Metabolism Differently, placentWhat is the role of human placental lactogen (hPL) in metabolic adjustments during pregnancy? (attemptmyexam.com)
  • For the implantation groups, uteri were obtained on D4, D5 and D6 (day of implantation) of pregnancy. (jri.ir)
  • Ectopic pregnancy is the result of a flaw in human reproductive physiology that allows the conceptus to implant and mature outside the endometrial cavity (see the image below), which ultimately ends in the death of the fetus. (medscape.com)
Genes2
  • The expression of Placental Lactobacilli-like genes such as Placental Gal4 in placentae may be increased in humans' later day use this link subsequent glucose conversion and further increase of HPL positive placental culture M5S-LIF. (attemptmyexam.com)
  • The above two groups of genes were intersected to obtain 72 genes for functional annotation analysis. (biomedcentral.com)
Samples1
  • 2002): Determination of arginine, asymmetric dimethylarginine, and symmetric dimethylarginine in human plasma and other biological samples by high-performance liquid chromatography. (ankara.edu.tr)
Optimal2
  • The optimal results are obtained with I caproate. (wikipedia.org)
  • Our Osteoblast Supplement consists of FCS (10 % [v/v] final concentration) which is specifically tested to support optimal growth of Normal Human Osteoblasts. (promocell.com)
Stimulation3
  • The stimulation protocol for these three IVF cycles is mild/mild/GnRHa long, which succeeded in obtaining 1/0/6 D3 embryos from 1/1/8 oocytes. (fortunejournals.com)
  • The patient continued her another two intracytoplasmic sperm injection (ICSI) cycle using mild stimulation protocol, and obtained 0/1 D5 blastocyst from 2/1 oocytes. (fortunejournals.com)
  • FBS (or BSA) can be substituted for HSA, however we do not recommend this as the FBS will lead to unpreferable immunologic stimulation of human macrophages. (promocell.com)
Suspension2
  • Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3′-dihexyloxacarbocyanine [DiOC 6 (3)] has been demonstrated. (ukrbiochemjournal.org)
  • Which plates does PromoCell recommend as 96-well U bottom suspension plates for chondrogenic differentiation of Human Mesenchymal Stem Cells? (promocell.com)
Myocardium2
  • In sections of human heart kisspeptin receptor-like immunoreactivity (2LI) was detected in myocardium from all areas of explanted hearts investigated (appropriate and left atria, appropriate and remaining ventricle and interventricular septum) and in sections of atrial appendage received from coronary artery bypass graft functions. (gpr44.com)
  • Inside the myocardium, receptor protein localised to both cardiomyocytes (Figure three A) and intramyocardial blood vessels, with cell specific markers determining localisation to both vascular clean muscle mass (Determine three D) and endothelial cells (Determine 3 G). Kisspeptin-like immunoreactivity was detected in vascular (Determine three J) and endocardial endothelial cells in addition to cardiomyocytes (Determine three M). Very similar expression designs ended up obtained in rat cardiovascular tissues. (gpr44.com)
Expression4
  • Transfection of cells with expression vectors containing the human telomerase reverse transcriptase (hTERT) maintains telomere length and effectively gives normal cells an unlimited life span in culture. (elsevierpure.com)
  • The expression of various smooth muscle/myometrium cell markers suggests that these cells may be an appropriate model system to study certain aspects of human myometrial function. (elsevierpure.com)
  • 49] observed an opposite impact with up-regulation of oxytocin receptor expression in primary culture of human myometrium exposed to IL-6. (globalcoalitionnetwork.shop)
  • To test the hypothesis that Galphas gene mutation may suppress the expression of TRH-R gene, we investigated whether hTRH-R gene expression is lower in human GH-secreting pituitary adenomas with Galphas mutation than in tumors without the mutation. (e-enm.org)
Concentration1
  • Concentration of individual proteins in the dialyzates obtained, closely resembled the composition of amniotic fluid. (researchgate.net)
Cell3
  • The obtained results confirm the use of DiOC 6 (3) for studying the influence of effectors on transmembrane potentials of intact cell compartments. (ukrbiochemjournal.org)
  • Myometrium and pup brain were harvested at 4·5 hr post intrauterine injection and Western blotting was used to detect whole cell phospho-p65 and COX-2. (mdmsignaling.com)
  • Serum from preeclamptic women, when applied to human umbilical vein endothelial cell cultures, produces no morphologic abnormalities in the cells but releases procoagulants, vasoconstrictors, and mitogens. (health.am)
Fetal1
  • In addition, the ductus arteriosus was obtained from nine fetal baboons, 28 fetal lambs and 4 neonatal lambs. (gla.ac.uk)
Interfere1
  • Structural disorders (eg, uterine fibroids) may cause abnormal bleeding because they interfere with the function of the uterine muscle (myometrium). (msdmanuals.com)
Receptors1
  • Another study has shown that PGE2 contracts longitudinal smooth mus- cle in human colon through EP1 receptors and both mouse ileum and colon through EP1 and EP3 receptors, whereas activation of EP2 receptor by butaprost reduces the tension in the three preparations [17]. (fxr-agonists.com)
Results2
  • 1. At the Coordinator Institution - The Pharmacology Department, Faculty of Medicine, University of Medicine and Pharmacy Craiova - Romania, In case of significant results on rat uterine strips, it will be remade all experiments on human myometrium fragments, preleaved, only after inform consent. (umfcv.ro)
  • The diameter of myocytes, assessed by photon correlation spectroscopy, was amounted 7-10 microm which is Consistent with the results obtained by using laser confocal microscopy. (kiev.ua)
Biological1
  • In conclusion, this approach can lead to new hypothesis-based medicine and provide a ''shortcut'' to obtain new biological insight. (peertechzpublications.com)
Maternal1
  • Maternal HCD was identified as a hormone in lactation that plays an important role in the development of click over here now glycogen storage in the myometrium. (attemptmyexam.com)
Parameters1
  • The effect of hPL in these parameters was then studied in correlation with other biochemical and biochemical parameters obtained on day 10 after menses. (attemptmyexam.com)
Function1
  • In contrast to all other human organs, which only begin to function after a sufficient period of development and maturation, the placenta has to control its own growth and develop full functionality at the same time. (zxc.wiki)
Investigate1
Normal1
  • Firstly, the GSE67463 dataset, including 25 ULMS tissues and 29 normal myometrium (NL) tissues, was downloaded from the public database. (biomedcentral.com)
Keywords1
Aorta1
  • In addition, specific binding was detected in the clean muscle mass of human aorta, human little coronary resistance vessels and rat aorta. (gpr44.com)
Study1
Middle1
Level1
  • The level of quality management per radiological facility was determined by scoring the quality indicators grouped into the following 3 main categories: human resource control, physical asset control, and safety of the work environment. (sajr.org.za)
Determine1
  • Western blotting discovered bands of the suitable size for the kisspeptin receptor rat 43 kDa (Determine 2A) mouse 75 kDa (Figure 2B) human 42 kDa (Determine 2C), that have been reliable with prior experiences [32]. (gpr44.com)