We describe here a method for DNA extraction from coastal water and a multiplex polymerase chain reaction (PCR) for O1 and O139 serogroups. (umd.edu)
12. Changing characteristics of Vibrio cholerae: emergence of multidrug resistance and non-O1, non-O139 serogroups. (nih.gov)
The polyclonal antibodies were prepared by injecting dosage of dead cells of V. cholerae to New Zealand white rabbits and following all the procedures the polyclonal antisera were prepared which was further used to identify the pathogenic O1 and O139 serogroups of V. cholerae fast and cost effectively. (ac.bd)
To determine whether this genomic motif and chitin-induced natural transformation might enable the exchange of serogroup-specific gene clusters between different O serogroups of V. cholerae, a strain of V. cholerae O1 El Tor was co-cultured with a strain of V. cholerae O139 Bengal within a biofilm on the same chitin surface immersed in seawater, and O1-to-O139 transformants were obtained. (nih.gov)
The causative organism, V. cholerae serogroups O1 and O139, is a short, curved, motile, aerobic, gram-negative bacillus that produces enterotoxin, a protein that induces hypersecretion of an isotonic electrolyte solution by the small-bowel mucosa. (msdmanuals.com)
However, mild or asymptomatic infection is much more common with the currently predominant El Tor biotype and with non-O1 and non-O139 serogroups of V. cholerae . (msdmanuals.com)
Cholera, caused by Vibrio cholerae serogroups O1 and O139, is one of the diseases reportable to the World Health Organization under the current International Health Regulations. (health.gov.au)
Cholera is an infectious, diarrheal, strictly human disease caused by bacteria from serogroups O1 and O139 of the species Vibrio cholerae. (pasteur.fr)
As previously described, E. coli strains harbouring the Stx 2e gene, usually belonging to serogroups O138, O139, O141 or O147 and expressing adhesins F18, are described as typical ethiological agents of OD. (pigprogress.net)
1]. There are 240 serogroups of V. cholera, only -O1 and O139 -are pathogenic. (researchkey.net)
Serogroup8
Testing at CDC identified the isolate as toxigenic V. cholerae serogroup O139, resistant to trimethoprim-sulfamethoxazole. (cdc.gov)
These strains could not be identified as any of the 138 known types of V. cholerae and have been designated as a new serogroup, O139 (5). (cdc.gov)
Thus, the O139 strains continue to evolve, and the adult population continues to be more susceptible to O139cholera, which suggests a lack of adequate immunity against this serogroup. (cdc.gov)
only a few cases have been attributable to O139 serogroup strains. (cdc.gov)
Since the initial emergence of V. cholerae O139 in 1992, we have monitored cholera outbreaks caused by this serogroup in Bangladesh and neighboring regions and have conducted several studies to characterize O139 strains. (cdc.gov)
For cholera surveillance of aquatic environments and ballast water, total DNA extraction, followed by V. cholerae PCR, and O1/O139 serogroup and tcpA/ctxA genes by multiplex PCR offers an efficient system, permitting risk analysis for cholera in coastal areas. (umd.edu)
Serogroup conversion of the O1 recipient by the O139 donor was demonstrated by comparative genomic hybridization, biochemical and serological characterization of the O-antigenic determinant, and resistance of O1-to-O139 transformants to bacteriolysis by a virulent O1-specific phage. (nih.gov)
No cases of serogroup O139 have been reported in Australia. (health.gov.au)
Strains12
The reemerged O139 strains belong to a single ribotype corresponding to one of two ribotypes that caused the initial O139 outbreak in 1993. (cdc.gov)
The new O139 strains carry a copy of the Calcutta type CTX Calc prophage in addition to the CTX ET prophage carried by the previous strains. (cdc.gov)
In this study, we examined the current epidemiology of cholera in Bangladesh and analyzed V. cholerae O139 isolated from the recent outbreak to investigate the origin of the recent epidemic strains as well as to characterize possible genetic changes in O139 vibrios that might have contributed to the recent resurgence of V. cholerae O139. (cdc.gov)
Characterization of Aeromonas trota strains that cross-react with Vibrio cholerae O139 Bengal. (nih.gov)
In the past decade the importance of non-O1 and non-O139 strains of Vibrio cholerae has been highlighted globally. (who.int)
A total of 95 800 stool samples submitted over 1999-2012 yielded 3668 strains of V. cholerae, of which 6% were non-O1 and non-O139 V cholerae. (who.int)
Illnesses caused by strains of V. cholerae other than toxigenic V. cholerae O1 or O139 should not be reported as cases of cholera. (cdc.gov)
V. cholerae non-O1/non-O139 strains are frequently isolated from aquatic ecosystems worldwide. (umd.edu)
The specificity of multiplex O1/O139 PCR was investigated by analysing 120 strains of V. cholerae, Vibrio and other Bacteria species. (umd.edu)
The choice of antibiotics is determined by the susceptibility patterns of the local strains of V cholerae O1 or O139. (medscape.com)
Out of 18 typical strains detected, the most of them belonged to O139 and were equipped with fimbrial adhesins F18. (pigprogress.net)
Bengal4
Vibrio cholerae O139 Bengal first emerged during 1992 and 1993 and caused large epidemics of cholera in Bangladesh, India, and neighboring countries ( 1 - 3 ). (cdc.gov)
Vibrio cholerae O139 Bengal: emergence of a new epidemic strain of cholera. (nih.gov)
Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples. (nih.gov)
Epidemiology & molecular biology of Vibrio cholerae O139 Bengal. (nih.gov)
Toxigenic1
This report documents the first case of cholera imported into the United States that was caused by this organism, the newly described toxigenic Vibrio cholerae O139 strain. (cdc.gov)
Serotype1
The methods for recognizing V. cholerae serotype O1 and O139 are crucial as it is sensitive as well as repeatable because up to 60%-75% of all cholera cases are subclinical. (ac.bd)
Polymerase chain re1
3. Clinical characteristics of non-O1/non-O139 Vibrio cholerae isolates and polymerase chain reaction analysis of their virulence factors. (nih.gov)
19931
I was involved in the epidemic caused by O139 in 1993. (sciencewatch.com)
Strain1
Furthermore, O139 vibrios may have originated from more than one progenitor strain ( 8 ). (cdc.gov)
Epidemic2
A total of 63 V. cholerae O139 isolates obtained from the recent cholera epidemic were analyzed. (cdc.gov)
The first epidemic of Vibrio cholerae O139. (nih.gov)
Laboratoire national2
Les donnees d'echantillons de selles ayant permis la croissance de V. cholerae non-OI et non-O139 isoles dans un laboratoire national specialise entre 1999 et 2012 onteteanalyseesetevalueesretrospectivement pour leur resistance a Tarn picilline, latetracycline,au chloramphenicol, au co-trimoxazole et a I'ofloxacine.Au total, 95800 echantillonsde selles soumis entre 1999 et 2012 ontproduit3668 souches de V. cholerae, parmi Iesquelles6%etaient des souches de V. cholerae non-OI etnon-O139. (who.int)
Les données d'échantillons de selles ayant permis la croissance de V. cholerae non-O1 et non-O139 isolés dans un laboratoire national spécialisé entre 1999 et 2012 ont été analysées et évaluées rétrospectivement pour leur résistance à l'ampicilline, la tétracycline, au chloramphénicol, au co-trimoxazole et à l'ofloxacine. (who.int)
Emergence1
Emergence of a new cholera pandemic: molecular analysis of virulence determinants in Vibrio cholerae O139 and development of a live vaccine prototype. (nih.gov)
Vulnificus1
The CDC provides recommendations on treatment regimens for V. vulnificus , V. parahaemolyticus and V. cholerae non-O1, non-O139 [ 4 ]. (biomedcentral.com)
Isolates1
ToxR genes were present in 18 of the isolates, but the lack of ctxA genes indicated that they were nonpathogenic O1/O139. (ac.bd)
Stool1
Data of stool specimens yielding growth of non-O1 and non-O139 V. cholerae isolated at a national referral laboratory from 1999 to 2012 were retrospectively analysed and evaluated for resistance to ampicillin, tetracycline, chloramphenicol, co-trimoxazole and ofloxacin. (who.int)
Infection4
Descriptions of the symptoms associated with V. cholerae O139 infection suggest it is indistinguishable from cholera caused by V. cholerae O1 and should be treated with the same rapid fluid replacement (7). (cdc.gov)
Comparison of O-specific polysaccharide responses in patients following infection with Vibrio cholerae O139 versus vaccination with a bivalent (O1/O139) oral killed cholera vaccine in Bangladesh. (harvard.edu)
Typical examples of the more than 30 infectious diseases emerging since 1970s are HIV/AIDS, Vibrio cholerae O139 infection, enterohemorrhagic Escherichia coli infection, severe acute respiratory syndrome (SARS), and avian influenza. (fujipress.jp)
Similarly, Vibrio cholerae O1 and O139 can also lead to toxico-infection. (safdynamics.com)
Cholera caused1
The epidemiology of cholera in Bangladesh changed again recently, and a large outbreak of cholera caused predominantly by V. cholerae O139 occurred in the capital city Dhaka and adjoining areas. (cdc.gov)
Antimicrobial2
This study aimed to evaluate the frequency and antimicrobial susceptibility profile of non-OI and non-O139 V. cholerae in Pakistan. (who.int)
13. In vitro antimicrobial effect of cefazolin and cefotaxime combined with minocycline against Vibrio cholerae non-O1 non-O139. (nih.gov)
19941
During 1994 to the middle of 1995, in most northern and central areas of Bangladesh, the O139 vibrios were replaced by a new clone of V. cholerae O1 of the El Tor biotype, whereas in the southern coastal regions the O139 vibrios continued to exist ( 4 - 6 ). (cdc.gov)
Bangladesh3
During March and April 2002, a resurgence of Vibrio cholerae O139 occurred in Dhaka and adjoining areas of Bangladesh with an estimated 30,000 cases of cholera. (cdc.gov)
During late 1995 and 1996, cases of cholera attributable to both V. cholerae O1 and O139 were again detected in various regions of Bangladesh. (cdc.gov)
The transient disappearance and reemergence of V. cholerae O139 in Bangladesh have raised questions regarding the origin of the reemerged O139 vibrios. (cdc.gov)
19991
Au total, 95 800 échantillons de selles soumis entre 1999 et 2012 ont produit 3668 souches de V. cholerae, parmi lesquelles 6 % étaient des souches de V. cholerae non-O1 et non-O139. (who.int)
Disease caused1
Cholera is an acute diarrheal disease caused by Vibrio cholerae O1 and O139. (usm.my)