• This and other data suggest that GPR99 is an important receptor for the in vivo actions of LTE4 but not LTD4 or LTC4 The GPR17 receptor, also termed the uracil nucleotide/cysteinyl leukotriene receptor, was initially defined as a receptor for LTC4, LTD4, and uracil nucleotides. (wikipedia.org)
  • A small number of drugs targeted to nucleotide metabolism, by virtue of their effect on folate biosynthesis and recycling, have been successfully used against apicomplexan parasites such as Plasmodium and Toxoplasma for many years, although resistance is now a major problem in the prevention and treatment of malaria. (pubmedcentralcanada.ca)
  • Replication of DNA and its transcription to the various RNA species necessitate a constant and substantial supply of the constituent nucleotides, a particularly demanding requirement in the case of rapidly dividing cells or pathogens, for example cancer cells or malaria parasites. (pubmedcentralcanada.ca)
  • The nucleotide molecule contains three functional groups: a base, a sugar, and a phosphate (see diagram). (encyclopedia.com)
  • The base editor variant, sgRNA protospacer and PAM consensus nucleotide, and incidence of base editing are indicated. (nih.gov)
  • If such intervention depletes the dNTP pool sufficiently, DNA replication is arrested, but even the induction of nucleotide imbalances can lead to misincorporation of base types during replication, leading to fatal genetic damage [ 1 ]. (pubmedcentralcanada.ca)
  • Figure 1: Quantitative base-induced DNA cleavage (QBIC) is a technique that allows DNA to be cleaved at any thymine site. (phys.org)
  • A research team led by Hiroki Ueda and colleagues from the Laboratory for Synthetic Biology at the RIKEN Quantitative Biology Center has now developed a chemical-based, non-enzymatic recombination technique that instead uses a DNA base analogue called 5-ethynyluracil to cleave DNA at any site containing the nucleotide thymine. (phys.org)
  • The technique developed by Ueda and his co-workers, which is called quantitative base-induced DNA cleavage (QBIC), starts with the generation of DNA fragments containing 5-ethynyluracil in place of thymine-two molecules with similar structures. (phys.org)
  • At each site, Rns recognizes asymmetric nucleotide sequences in two regions of the major groove and binds along one face of the DNA helix. (asm.org)
  • Analysis of Rns-nucleotide interactions at each site suggests that binding sites for Rns and related virulence regulators are not easily identified because they do not bind palindromic or repeated sequences. (asm.org)
  • In this chemical bath, all the nucleotides containing 5-ethynyluracil become cleaved, introducing gaps near the cleaved ends. (phys.org)
  • they found that GPR17-bearing cells did not respond to these CysLTs or nucleotides but did find that cells expressing both CysLTR1 and GPR17 receptors exhibited a marked reduction in binding LTC4 and that mice lacking GPR17 were hyper-responsive to igE-induced passive cutaneous anaphylaxis. (wikipedia.org)