• wgSNP comparison is performed to identify single nucleotide polymorphisms (SNPs) that distinguish isolates in a genotype-matched cluster. (cdc.gov)
  • SNPs result from mutations at a single position in the DNA sequence. (cdc.gov)
  • JBS Sequencing polymerase or Thermosequenase™) as 3'-end chain terminators in Sanger sequencing [1] (Fig. 1) and single nucleotide polymorphisms (SNPs) genotyping by single base pair extension (SBE) [2] (Fig. 2). (jenabioscience.com)
  • The single basepair extension (SBE) assay is a reliable method for the detection of a priori known locations of single nucleotide polymorphisms (SNPs) [2] . (jenabioscience.com)
  • 2020). The two extensively studied non-synonymous DBP single nucleotide polymorphisms (SNPs) rs7041 and rs4588 occur in different populations (Santos et al. (h3africa.org)
  • Single nucleotide polymorphisms (SNPs) are single base pair mutations at specific locations in the coding and non-coding regions of the genome that are found in more than 1% of a population [1] . (jenabioscience.com)
  • Single nucleotide polymorphisms (SNPs) are detected by enzymatic elongation of a primer by a SNP-complementary, fluorescently labeled ddNTP. (jenabioscience.com)
  • Although the functional significance of nearly all of this sequence is unknown, 387 of the novel contigs fall within 315 distinct protein-coding genes, and the rest appear to be intergenic. (nih.gov)
  • A total of 110 clinical isolates of A. baumannii , collected in a recent 2-year period, were tested for carbapenem antibiotic susceptibility, followed by a molecular analysis of carbapenemase genes. (frontiersin.org)
  • To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. (embl-heidelberg.de)
  • For this, they collected 514 blood samples of malaria-infected patients from the eight malaria-endemic states in the country, isolated DNA from them and amplified the three genes. (thehindubusinessline.com)
  • The genes were then sequenced and nucleotide composition of the samples was compared. (thehindubusinessline.com)
  • Among the three genes, nucleotide composition of glutamate dehydrogenase was almost the same across the samples. (thehindubusinessline.com)
  • In the present study, the genetic variability of E. dispar strains based on the SREDP gene locus was analyzed using RFLP-nested PCR and sequencing of the obtained genes. (scialert.net)
  • The relationship between functional single nucleotide polymorphisms (SNP) of the FCGR2A and FCGR3A genes and the response to anti-tumour necrosis factor (TNF)α therapy (infliximab) in patients with rheumatoid arthritis (RA) was assessed. (bmj.com)
  • Blood and the pleural fluid (purulent aspect) samples were subjected to qPCR (16S rRNA and dsb genes) and sequencing, which identified Ehrlichia canis and Anaplasma platys coinfection. (bvsalud.org)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • This multifaceted analysis showed a similarity between the sequences from the patient and the dentist that was comparable to what has been observed for cases that have been epidemiologically linked (Los Alamos National Laboratory, unpublished data). (cdc.gov)
  • However, sequencing revealed only five animals, including the bovine which died, with a similarity of the amino acid sequences with AmRio 2 strain of A. marginale . (scielo.br)
  • Nucleotide sequence similarity dot plot of the de novo transcriptome of Plodia interpunctella granulovirus (this study) versus the assembled Plutella xylostella granulovirus genome (NCBI reference sequence NC_002593.1). (biomedcentral.com)
  • When the biologists get an unknown sequence, in general they would compare this unknown sequence (denoted as query sequence) with the known database of sequences (denoted as database sequences) to find the similarity scores and then identify the evolutionary relationships among them. (hindawi.com)
  • Find regions of similarity between this sequence and other sequences using BLAST. (nih.gov)
  • Amplicons were cloned, sequenced, and used in BLAST searches against the NCBI nucleotide database to determine the closest fungal relative based on sequence similarity. (cdc.gov)
  • Centers for Disease Control and Prevention, Fort Collins, Colo- nucleotide identity with ZIKV. (folkhalsomyndigheten.se)
  • The complete nucleotide sequence of 15,611 bases has been determined, and the gene structures were examined. (embl-heidelberg.de)
  • HBV is characterized by a genetic heterogeneity and 8 genotypes (A to H) can be classified based on comparison of complete HBV genomes and according to the criterion of ≥ 8% differences in the complete nucleotide sequence of the viral genome [4-6]. (who.int)
  • Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)
  • Although isolates of Phylogenetic analysis on the TPI sequences confirmed the G. duodenalis from humans and various animals are mor- formation of distinct groups. (cdc.gov)
  • Although the viral sequences from the dentist and the patient could be distinguished from each other, they were closer than what has been observed for pair-wise comparisons of sequences taken from the other North American isolates studied (3). (cdc.gov)
  • Genetic relatedness of the isolates was characterized by multilocus sequence typing. (frontiersin.org)
  • Most of these CRAB isolates (77.62%) were classified as clone complex 92 (CC92), and sequence type (ST) 92 was the most prevalent STs, followed by ST195, ST136, ST843, and ST75. (frontiersin.org)
  • Each of these 2,690 loci are analyzed and assigned a number such that isolates that have the same sequence at a locus will have the same number assigned for that locus. (cdc.gov)
  • Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
  • Thus, one of the sequences reported here would be a logical choice as a reference strain for inclusion in future studies of North American HSV-2 isolates. (emmes.com)
  • Our study provides scientific evidence for the conserved nature of glutamate dehydrogenase sequences in Indian isolates which can be used as a potential biomarker for diagnosis of malaria," said Dr. Praveen Kumar Bharti, leader of the research team. (thehindubusinessline.com)
  • The polymorphism of the Serine-Rich Entamoeba dispar Protein (SREDP) gene among 8 isolates obtained from Iranian cyst carriers were analyzed by a nested PCR-RFLP followed by sequencing of the PCR products. (scialert.net)
  • From those isolates, six distinct DNA patterns were observed after PCR-RFLP of the nested PCR, whereas sequencing showed 8 different patterns among the isolates. (scialert.net)
  • Ghosh reported 5 different SREDP sequencing patterns from 5 isolates and showed high genetic variation among the E. dispar in compare with E. histolytica (Ghosh et al . (scialert.net)
  • The DNA was extracted for PCR amplification in this study after recovery and re-cultivation of the frozen isolates in Robinson medium (Robinson, 1968). (scialert.net)
  • Sequencing was conducted on 23 influenza (H1N1)pdm09 isolates that were recovered from positive samples. (who.int)
  • There were two different mismatches in the probe target sites of the HA gene sequences of all isolates ( n = 23) with additional mismatches only at position 7 (template binding site) identified for all eight negative real-time RT-PCR isolates. (who.int)
  • What is Whole Genome Sequencing (WGS) and what analyses are performed using WGS data? (cdc.gov)
  • Whole Genome Sequencing (WGS) generates DNA sequence data for the entire M. tuberculosis genome, which can be used for various applications. (cdc.gov)
  • The purpose of this training video is to provide state and local tuberculosis (TB) control program staff with information from CDC's Division of TB Elimination (DTBE) on the methods for performing whole-genome single nucleotide polymorphism (SNP) comparison and building phylogenetic trees. (cdc.gov)
  • Tuberculosis whole-genome single nucleotide polymorphism (SNP) Training. (cdc.gov)
  • In 2018, CDC's Division of TB Elimination (DTBE) began universal whole-genome sequencing (WGS) (i.e., sequencing an M. tuberculosis isolate for each U.S. case of culture-confirmed TB). (cdc.gov)
  • A training module on "Whole-genome sequencing for investigation of recent TB transmission in the United States: Current uses and future plans" is available here. (cdc.gov)
  • We used next-generation sequencing techniques to determine whole-genome sequences of four viral samples from two subjects in the Herpevac Trial. (emmes.com)
  • Each of the HSV-2 genome sequences was initially obtained using next-generation sequencing and completed with Sanger sequencing. (emmes.com)
  • The enzymatic dideoxy chain termination sequencing method of Sanger [1] relies on the linear amplification of a single-stranded template DNA using a 5'-fluorescently labeled primer and a modified Taq polymerase. (jenabioscience.com)
  • We genotyped the DBP gene by Sanger sequencing and the single nucleotide polymorphisms were identified using the BioEdit tool. (h3africa.org)
  • EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • DNA isolation, genotyping of simple tandem repeat polymorphisms, PCR amplification and sequencing were performed using standard techniques 2 and all simple tandem repeat polymorphisms were amplified in the presence of 10% glycerol and 5% DMSO after labelling one oligonucleotide primer with 32 P and analysed on a 6% polyacrylamide gel. (bmj.com)
  • An analysis of encoded amino acid sequence by triplets with substituted nucleotides revealed that 8 changes were polymorphic, and 1 was a mutation substituting GTG (valine) for ATG (methionine) at 236 position. (aaem.pl)
  • Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
  • Approximately 80% of patients with CS, 60% patients with BRRS, 20% of patients with PS/PSL syndrome will have a PTEN mutation identifiable by sequencing. (cincinnatichildrens.org)
  • Deletion/duplication analysis of PTEN will detect a mutation in about 10% of individuals with Bannayan-Riley-Ruvalcaba syndrome. (cincinnatichildrens.org)
  • Mitochondrial DNA is 10 times more prone to mutation than somatic DNA. (medscape.com)
  • The process of agent-induced mu- the mutation - either through faulty not a mutation and general y does tagenesis consists of three parts: the DNA repair of the mutagen-induced not alter the linear sequence of nu- induction of DNA damage, the sen- or spontaneous DNA damage, or cleotides. (who.int)
  • A mutation is defined as a sing of the DNA damage by the cel by replicating past the unrepaired change in the sequence or number (the DNA damage response), and DNA damage, thereby introducing a of nucleotides in the DNA. (who.int)
  • cell detects it by means of the DNA result in a mutation. (who.int)
  • Other types repair the damage but instead pro- mutagens produce DNA damage, of DNA damage are oxidized or frag- cess it into a mutation, or direct the and they might more appropriately mented bases and the intercalation cell to undergo apoptosis. (who.int)
  • possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)
  • The most common mutation in FAP is a deletion of five building blocks of DNA (nucleotides) in the APC gene. (medlineplus.gov)
  • This mutation changes the sequence of the building blocks of proteins (amino acids) in the resulting APC protein. (medlineplus.gov)
  • Fungal rRNA sequencing of indoor and occupational air samples. (cdc.gov)
  • In this pilot study, rRNA sequencing was utilized to examine the diversity of fungal species present in indoor and occupational environments. (cdc.gov)
  • For molecular analyses, DNA was collected and rRNA sequences were amplified with universal fungal primers. (cdc.gov)
  • Design and test primers for this sequence using Primer-BLAST. (nih.gov)
  • Recently, metagenomics a new approach to identify culturable and non culturable bacteria with the help of 16S rDNA sequences isolate total DNA from HCH contaminated soil and amplified 16S rDNA through universal primers and confirmed its total population with denaturing gradient gel electrophoresis and sequencing. (ispub.com)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • In the second step, the temperature decreases (45-60°C), depending on the primers, to permit annealing (strengthening) of the target DNA primers. (medscape.com)
  • We describe a general approach to rapidly obtain the DNA sequence encoding the variable region of any immunoglobulin chain using the polymerase chain reaction and a mixture of upstream primers corresponding to the leader sequence, and one downstream primer designed from the conserved nucleotide sequence of the constant region. (lu.se)
  • Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are required by the histone chaperone FACT complex to incorporate nucleosomes containing the histone variant H2A.X. Further, phosphorylation of H2A.X at S139 (γ-H2AX) is required for repair-mediated DNA demethylation and transcription activation. (nature.com)
  • Active DNA demethylation also requires GADD45A (growth arrest and DNA damage protein 45 alpha) and TET1 (ten-eleven translocation methylcytosine dioxygenase 1), which sequentially oxidize 5-methylcytosine (5mC) to 5-carboxylcytosine (5caC) 18 , 19 and are cleared through DNA repair mechanisms. (nature.com)
  • Sequence homology searches identify PhnC, PhnK, PhnL, and, possibly, PhnN proteins as members of nucleotide-binding proteins of the binding protein-dependent transport systems. (embl-heidelberg.de)
  • Correlation between a coat protein amino-terminal sequence and a ribosome-binding DNA sequence from phiX 174. (wikidata.org)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • We find useful ranges of properties for DNA polymerase-based recorders, providing guidance for future protein engineering attempts. (mit.edu)
  • Analysis of the protein structure of this gene revealed that it folded into similar protein structure across the samples, confirming that it could be a potential biomarker for malaria. (thehindubusinessline.com)
  • The nucleotide sequences of Serine-Rich Entamoeba histolytica Protein (SREHP) gene have already exhibited stable and significant polymorphism in the gene studies. (scialert.net)
  • The first nucleotide sequences have been shown the stable and significant polymorphism in the Strain Specific Gene (SSG) and the Serine-Rich E. histolytica Protein (SREHP) gene. (scialert.net)
  • This precision and versatility mean that the approach could be applied to molecules beyond DNA, such as protein building blocks called peptides, which could help advance proteomics as well as biomedical and clinical research. (scitechdaily.com)
  • And here opens a new Window, where we can see the amino acid sequence of the File- of the protein fbaB. (futurelearn.com)
  • Little genetic drift was detected in viruses shed by the same subjects following repeated reactivation events, suggesting strong selective pressure on the viral genome to maintain sequence fidelity during reactivations from its latent state within an individual host. (emmes.com)
  • A team of researchers from Indian Council of Medical Research's Jabalpur-based National Institute of Research in Tribal Health (NIRTH) has identified a genetic sequence in the body of malaria parasite that promises to help develop a more sensitive diagnostic test for the disease. (thehindubusinessline.com)
  • Scientists can determine DNA's sequence of nucleotides - which encodes genetic information - by analyzing how each one perturbs this current as it passes through. (scitechdaily.com)
  • Whole exome sequencing was carried out on an Italian family with moyamoya-complicated neurofibromatosis type 1 to identify putative genetic modifiers independent of the NF1 locus and potentially involved in moyamoya pathogenesis. (cancerindex.org)
  • Without a clear pattern of signs and symptoms, the clinical suspicion may be insufficient to prompt genetic analysis. (medscape.com)
  • Histone variants differ from the canonical histones in their amino acid sequence and have specific and fundamental functions that cannot be performed by canonical histones. (nature.com)
  • Analysis of expression and amino acid sequence of the allergen Mag 3 in two species of house dust mites-Dermatophagoides farinae and D. pteronyssinus (Acari: Astigmata: Pyroglyphidae). (aaem.pl)
  • In this work, we aimed to detect expression of this important allergen and for the first time analyze to the amino acid sequence in other species of house dust mite - Dermatophagoides pteronyssinus [DP]. (aaem.pl)
  • However, the presence of amino acid sequence difference in this allergen might suggest that there exist other isoforms which can make difficult both diagnosis as well as immunotherapy in persons who produce allergic response to this allergen. (aaem.pl)
  • The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
  • The next thing we're going to learn is to visualise the nucleotides and amino acid sequence of this gene in a separate Window. (futurelearn.com)
  • Total 38 nucleotides and 36 proteins sequences of linA and linB were used for constructing the phylogeny. (ispub.com)
  • Moreover, speci®c hy- found in narrow minor groove regions in a variety dration patterns seem to play a role in nucleotide sequence recognition by proteins (Otwinowski of oligonucleotide duplexes (Prive et al. (lu.se)
  • Usually, the NOE and NMRD in B-DNA is expected to be less ``integral'' than methods can only provide bounds on the residence water molecules buried inside proteins. (lu.se)
  • DNA Electronics develops scalable semiconductor technology for real-time nucleic acid detection, "which enables faster, simpler, and more cost-effective DNA analysis platforms," it said in a statement. (genomeweb.com)
  • WHO Collaborating Centre for Orthopoxvirus Diagnosis and Repository for Variola Virus Strains and DNA, State Research Centre of Virology and Biotechnology "VECTOR", Koltsovo, Novosibirsk Region, Russian Federation. (who.int)
  • The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
  • We assess our methods by assembling two Staphylococcus aureus strains that were sequenced on the Illumina platform. (nih.gov)
  • To determine the relatedness of the HIV strains from both persons, DNA was extracted from their peripheral blood mononuclear cells (PBMC). (cdc.gov)
  • DNA, serological detection of variola virus, antiviral agents and animal models of smallpox. (who.int)
  • This analysis enabled detection of 9 single base changes. (aaem.pl)
  • Subsequent detection of the incorporated ddNTP is performed by mass spectrometry of the extended primer, thus revealing the nucleotide base at that position on the template strand [3] . (jenabioscience.com)
  • What's particularly exciting is that this increased detection capability with SICS may be transferable to other solid-state and biological nanopore methods, which could significantly improve diagnostic and sequencing applications," Leitão says. (scitechdaily.com)
  • The sensitivity of DNA sequencing is over 99% for the detection of nucleotide changes, small deletions and insertions in the regions analyzed. (cincinnatichildrens.org)
  • The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)
  • 2001) recently discussed phylogenetic relationships inferred from parsimony analysis of nucleotide sequences of the internal transcribed spacer regions (ITS) of the 18S-26S nuclear ribosomal DNA of 34 species of Salsola and related genera ( Halothamnus Jaubert & Spach, Climacoptera Botschantzev, Girgensohnia Bunge, Halocharis Moquin-Tandon, and Haloxylon Bunge) and four species from representative outgroups (tribes Camphorosmeae and Atripliceae). (efloras.org)
  • These ribosomal sequences was functional for the phylogenetic analysis and molecular taxonomic of any bacteria. (ispub.com)
  • Using 100 bp paired-end reads and minimal manual curation, we produce a finished genome sequence for the previously undescribed isolate SGH-10-168. (nih.gov)
  • The sequence of each subject's well-documented primary isolate was compared with the sequence of the isolate from their fifth or sixth episode of recurrent disease. (emmes.com)
  • 1999) From gels to chips: Minisequencing primer extension for analysis of point mutations and single nucleotide polymorphisms. (jenabioscience.com)
  • 2011) Clinical relevance of multiple single-nucleotide polymorphisms in Pneumocystis jirovecii Pneumonia: development of a multiplex PCR-single-base-extension methodology. (jenabioscience.com)
  • Genotyping of FCGR2A -R131H and FCGR3A -F158V polymorphisms was performed by allele-specific PCR and PCR sequence-based typing, respectively. (bmj.com)
  • Needleman and Wunsch [ 1 ] proposed a dynamic programming method (abbreviated to NW algorithm) to solve the global alignment problem between two sequences in 1970. (hindawi.com)
  • Phylogenetic Analysis and Paleontology Columbia University Press, New York. (berkeley.edu)
  • On the basis of 16S rDNA phylogenetic analysis of bacteria was done. (ispub.com)
  • The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I. (wikidata.org)
  • 7- 9 All reported mutations are single nucleotide alterations leading to splice site, missense, nonsense, or frameshift mutations, or intra-exonic deletions and insertions of up to four nucleotides, which have been detected through exonic PCR amplifications and sequencing. (bmj.com)
  • In contrast to the abundance mutations in SDHB and SDHD , only a single multiply affected family and an isolated case, containing a single nucleotide initiation codon and a splice site mutations in SDHC , respectively, have been described by Niemann et al . (bmj.com)
  • Using a DNA polymerase to record intracellular calcium levels has been proposed as a novel neural recording technique, promising massive-scale, single-cell resolution monitoring of large portions of the brain. (mit.edu)
  • Human microbiome analysis can be used in combination with traditional human DNA analysis techniques such as short-tandem-repeat sequencing and single nucleotide polymorphism analysis to provide more robust results in cases where people are suspected of having been involved with bioterrorism or biocrime. (news-medical.net)
  • 2000) Single nucleotide polymorphism analysis by MALDI-TOF mass spectrometry. (jenabioscience.com)
  • 2017 to March 2018, there was circulation of both detect a single target in a very small concentration of DNA influenza A and B, with influenza A(H1N1)pdm09 again or RNA because it uses a fluorescent dye that binds to predominating in Viet Nam. (who.int)
  • The predicted size of the COMT transcript was 1.8-2.0 kb, which could be confirmed from Northern hybridization analyses of the isolated rat liver mRNA. (lu.se)
  • G. microti and nucleotide sequence characterization of The taxonomy of Giardia at the species level is compli- amplified TPI fragment. (cdc.gov)
  • The relatedness of the sequences was analyzed by several computer-based methods in collaboration with Los Alamos National Laboratory. (cdc.gov)
  • Human identification using DNA-based methods provides analysis of stable, inherited biomarkers that are related to an individual's ancestry and phenotype, for example. (news-medical.net)
  • Phylogenetic analyses using Bayesian methods recovered a monophyletic grouping of all Dendrobium species (D. nobile, D. huoshanense, D. officinale, D. pendulum, D. strongylanthum and D. chrysotoxum). (plos.org)
  • however, the etiological agents remain uncharacterized due to the limitations of traditional sampling and analysis methods. (cdc.gov)
  • CONCLUSIONS: Compared to traditional methods of analysis, our data demonstrate a much wider diversity of fungi that may possibly contribute to personal exposure and adverse health effects in home and occupational environments. (cdc.gov)
  • A secondary goal that should be sought by a bioinformatics facility is to make algorithms and analysis methods accessible for non-computational biologists. (lu.se)
  • In bioinformatics, the sequence alignment has become one of the most important issues. (hindawi.com)
  • Cybulski TR, Boyden ES, Church GM, Tyo KEJ, Kording KP (2017) Nucleotide-time alignment for molecular recorders, PLoS Computational Biology 13(5):e1005483. (mit.edu)
  • The primer and probe sets in the HA gene were checked for mismatches, and phylogenetic analyses were performed to determine the molecular epidemiology of these viruses. (who.int)
  • Logistic regression analyses were used to determine factors independently associated with polytobacco use among men only (due to low use among women). (who.int)
  • Primer extension analysis showed that the transcription start point of the rat liver COMT mRNA was 450 bp upstream from the. (lu.se)
  • It relies on the extension of a primer, designed to bind one nucleotide upstream of the polymorphic spot, by a matching ddNTP. (jenabioscience.com)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
  • Over recent decades, advances in technologies such as massive parallel sequencing, atomic force microscopy, mass spectrometry and bioinformatics now enable high-level microbial characterization for various applications including the identification of perpetrator(s), body fluid assessment, and the tracking of infectious agents. (news-medical.net)
  • 3, 10 However, analyses of SDHC in four series of patients with paraganglioma or pheochromocytoma 6, 8, 11, 12 yielded no definitive SDHC mutations. (bmj.com)
  • Promoter analysis of PTEN will identify mutations in about 10% of patients with Cowden syndrome. (cincinnatichildrens.org)
  • A Pho box-like promoter sequence is also found upstream of the gene cluster starting at phnA, which is consistent with the observation of phosphate regulation of the Phn+ response. (embl-heidelberg.de)
  • HBV-DNA was detectable in sera from 3 patients out of the 40 patients who were positive for hepatitis B core antibody. (who.int)
  • Specific DNA present in small amounts in a clinical specimen are amplified by PCR so they become detectable. (medscape.com)
  • About half (54.3%) of the patients had elevated alanine aminotransfe-rase levels and detectable serum HBV DNA. (who.int)
  • Such tools are also used to characterize the human microbiome as an application in forensics for human identification, the characterization of bodily fluids, and the analysis of time-since-death composition. (news-medical.net)
  • Deletion analysis demonstrated that at least 13 kilobases of DNA information is required for E. coli to express the phosphonate utilization phenotype (Phn+). (embl-heidelberg.de)
  • Information regarding gene content, polycistronic transcription units, sequence insertion or deletion, transition or transversion, and nucleotide repeats may help resolve evolutionary relationships in the kingdom Plantae (Viridiplantae) 6 , 7 , 8 . (plos.org)
  • The identity of the clone and the accuracy of the sequence was verified by direct aa sequencing of the tryptic peptides derived from the purified rat liver enzyme. (lu.se)
  • Finding a solution for sequencing peptides has been a significant challenge due to the complexity of their "license plates", which are made up of 20 characters ( amino acids ) as opposed to DNA's four nucleotides," says Radenovic. (scitechdaily.com)
  • A comparative taxonomic and phytogeographic analysis (S. L. Mosyakin 2002) also suggests the place of origin of the Salsola generic aggregate is somewhere in the Tethyan region of south-central Asia (probably northern coasts of the ancient Tethys, or adjacent inland lacustrine habitats). (efloras.org)
  • Here, we report the complete cp genome sequence of Dendrobium nobile from Northeast India (Orchidaceae, Asparagales), bearing the GenBank accession number KX377961, which will provide valuable information for future research on orchid genomics and evolution, as well as the medicinal value of orchids. (plos.org)
  • Subsequent fluorescent visualization of the product reveals the incorporated nucleotide and hence the SNP genotype. (jenabioscience.com)
  • Contamination of reagents or specimens with target DNA from the environment must be avoided because such contamination can produce false-positive results. (medscape.com)
  • In March, Ion Torrent launched its electronic sequencer that reads DNA on a semiconductor chip by measuring the release of protons as nucleotides get incorporated by DNA polymerase. (genomeweb.com)
  • In contrast to 2H and 17O, the 1H relaxation dispersion is dominated by labile DNA protons and therefore provides little information about DNA hydration. (lu.se)
  • Intraspecific and interspecific pairwise distances (K2P) were 0-0.5% and 4.9-5.2%, respectively, and each species was recovered as a distinct, well supported group of sequences (i.e., species) in the ML analysis. (bioone.org)
  • An analysis including barcodes of Afrotropical Eccopsis (four species), Afrotropical Paraeccopsis (one species), and Neotropical Eccopsis (two species) failed to recover Eccopsis as monophyletic. (bioone.org)
  • Oligo Synthesizers are machines used to custom-build DNA molecules to contain a particular sequence of nucleotides. (digitaljournal.com)
  • Oligo Synthesizers can create specific DNA molecules for use in the treatment of a variety of diseases by replacing a faulty or damaged section of DNA with a repaired section. (digitaljournal.com)
  • Currently, the passage of molecules through a nanopore and the timing of their analysis are influenced by random physical forces, and the rapid movement of molecules makes achieving high analytical accuracy challenging. (scitechdaily.com)
  • Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)
  • The amplified segment may be observed using electrophoretic gel or can be identified by Southern blot analysis, using specific DNA probes for that segment. (medscape.com)
  • Nucleotide clusters in deoxyribonucleic acids. (wikidata.org)
  • This versatile method could greatly impact DNA analysis, proteomics, and clinical research. (scitechdaily.com)
  • The synthesis of the complementary DNA strand starts at the specific priming site and ends with the incorporation of a chain-terminating ddNTP that is randomly introduced instead of its corresponding dNTP . (jenabioscience.com)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • Wu's son, Ray J. Wu, became the Liberty Hyde Bailey Professor of Molecular Genetics and Biology at Cornell University, and developed the first method for sequencing DNA and studying transgenic plants. (wikipedia.org)
  • Analysis of phenylpropanoid metabolites in transgenic tobacco showed that lignin content was affected when PAL activity is reduced to 20-25% of wild type levels (Bate et al . (scialert.net)
  • The possible hypoxia response element (HRE) sequences in 5' non-coding Region before ATG start codon of Sema4D were screened, followed by point mutagenesis and luciferase assay analysis. (jcancer.org)
  • The process of mutagenesis be called DNA-damaging agents. (who.int)
  • In the context of the process of a necessary role in the potential mu- agents were found to be mutagens, mutagenesis described above, the tagenic activity of such compounds, leading to the conclusion that carcin- term "mutagen" refers to an agent or even that DNA, as opposed to ogens were general y not mutagenic. (who.int)
  • Biswal D, Konhar R, Debnath M, Parameswaran S, Sundar D, Tandon P. Chloroplast Genome Sequence Annotation of Dendrobium nobile (Asparagales: Orchidaceae), an Endangered Medicinal Orchid from Northeast India. (plos.org)
  • Our data support the concept that chromatin opening during transcriptional initiation involves intermediates with DNA breaks that subsequently require DNA repair mechanisms to ensure genome integrity. (nature.com)
  • Direct determination of DNA nucleotide sequences: structure of a fragment of bacteriophage phiX172 DNA. (wikidata.org)
  • The nucleotide and amino acid sequences of the N (5') terminal region of gene G of bacteriophage phiphiX 174. (wikidata.org)
  • The structure of the DNA of bacteriophage x-174. (wikidata.org)
  • Studies on bacteriophage fd DNA. (wikidata.org)
  • Finds sub-sequences or patterns in the sequence and highlights the matching regions. (nih.gov)
  • Finds sub-sequence or patterns in the sequence and highlights the matching region. (nih.gov)
  • The practice of ongoing, systematic environmental monitoring and analysis is recommended because it can aid in identifying trends, patterns, or associations that can point to suspected factors which may contribute to on-farm contamination. (cdc.gov)