While CNVs usually cover large DNA sequence variations, single nucleotide polymorphisms or SNPs are random, single base substitutions found throughout the genome. (jove.com)
These features of the RC-PCR make the technology a unique application to target single nucleotide polymorphisms (SNPs) in fragmented and low copy number DNA and yield results from samples in which no or limited data are obtained with standard DNA typing methods. (ojp.gov)
To compare single nucleotide polymorphisms (SNPs) in the 3′-untranslated region (3′UTR) of human leukocyte antigen (HLA)-G in placentas between spontaneous preterm birth and preeclampsia pregnancies. (biomedcentral.com)
Recently, associations between preeclampsia and SNPs in the 3′ untranslated region (3′UTR) of HLA-G have been observed [ 13 ]. (biomedcentral.com)
The aim of the present study was to determine the association of -344T/C and -470C/T Single Nucleotide Polymorphisms (SNPs) of the CYP11B2 and their resulted haplotypes with EH and Plasma Aldosterone Concentration (PAC) in southern population of Iran. (ephysician.ir)
What are single nucleotide polymorphisms (SNPs)? (medlineplus.gov)
Single nucleotide polymorphisms, frequently called SNPs (pronounced "snips"), are the most common type of genetic variation among people. (medlineplus.gov)
They occur almost once in every 1,000 nucleotides on average, which means there are roughly 4 to 5 million SNPs in a person's genome. (medlineplus.gov)
When SNPs occur within a gene or in a regulatory region near a gene, they may play a more direct role in disease by affecting the gene's function. (medlineplus.gov)
Parallel independent component analysis (parallel-ICA) was used to examine the relationship between language-related regions extracted from resting-state fMRI and 16 single nucleotide polymorphisms (SNPs) spanning 5 RD-related genes. (nih.gov)
In previous research, eleven SNPs in the promoter regions of four candidate genes were selected. (umd.edu)
The aim of this study was to identify any impact of CYP2A13 single-nucleotide polymorphisms (SNPs) on CYP2A13 expression in human lung. (aspetjournals.org)
To date, more than 20 single-nucleotide polymorphisms (SNPs) have been identified in CYP2A13 (Zhang et al. (aspetjournals.org)
Sequences9
Find regions of similarity between this sequence and other sequences using BLAST. (nih.gov)
Finds sub-sequences or patterns in the sequence and highlights the matching regions. (nih.gov)
The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
In some PCR reactions multiple sequences have identical nucleotide sequences, for example if five PCR products have identical sequences a 5 will be found in this column. (cdc.gov)
The DNA sequences including the 3′UTR region of HLA-G were amplified via PCR in a final reaction volume of 50 μl, using the Multiplex PCR Master Mix (Qiagen, Valencia, CA, USA) and 10 pmol of each primeralong with 100 ng of genomic DNA using SimpliAmp™ Thermal Cycler(Life Technologies, Carlsbad, CA, USA). (biomedcentral.com)
The possible hypoxia response element (HRE) sequences in 5' non-coding Region before ATG start codon of Sema4D were screened, followed by point mutagenesis and luciferase assay analysis. (jcancer.org)
This technology involves breaking the entire genome into small segments, sequencing the segments, and then reassembling the sequences using intensive computational techniques to provide the base-by-base sequence of the entire genome or more limited regions, such as the expressed portion of the genome known as the exome. (msdmanuals.com)
Promoter region sequences were extracted oil palm genome spanning from 1500 nucleotides (nt) upstream to 1000 nt downstream of every annotated transcription start sites (TSS). (bvsalud.org)
Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
Sequence9
Finds sub-sequence or patterns in the sequence and highlights the matching region. (nih.gov)
Links in the FEATURES table will also highlight the corresponding region of the sequence. (nih.gov)
In addition, a third conserved intronic sequence that is known to be functionally important in splicing is the so-called branch point site (BPS) which is usually located very close to the end of the intron, at most 40 nucleotides before the terminal dinucleotide. (ijbs.com)
Complete nucleotide sequence of the 5' noncoding region of rabbit beta-globin mRNA. (wikidata.org)
1% difference in nucleotide sequence from the prototype vaccine virus ( 1 ). (cdc.gov)
Our study describes the sequence characteristics of 5' non-coding region of Sema4D, enhances our understanding of the regulatory mechanism of Sema4D and benefits the development of a possible anti-angiogenesis therapeutic strategy for malignancies. (jcancer.org)
The nucleotide sequence of the methylamine utilization (mau) gene region from Methylobacterium extorquens AM1 was determined. (caltech.edu)
Anticipated orthologous ORFs in the regions above were defined as BLAT (Kent 2002) or SSearch (Pearson and Lipman 1988) sequence matches. (ubc.ca)
Array comparative genomic hybridization (aCGH) is a type of microarray now routinely used to identify deleted or duplicated regions of DNA sequence in specific chromosomes on a genome-wide basis. (msdmanuals.com)
Cyclic4
3′,5′-cyclic-nucleotide phosphodiesterases (EC 3.1.4.17) are a family of phosphodiesterases. (wikipedia.org)
The systematic name for this enzyme is 3′,5′-cyclic-nucleotide 5'-nucleotidohydrolase. (wikipedia.org)
3′,5′-cyclic-nucleotide phosphodiesterases in rod cells are oligomeric, made up of two heavy catalytic subunits, α (90 kDa) and β (85 kDa,) and two lighter inhibitory γ subunits (11 kDa each). (wikipedia.org)
Ligand-binding domain subregions contributing to bimodal agonism in cyclic nucleotide-gated channels. (sfu.ca)
Single12
It is a hepatotropic, approximately 7.2 kb single-stranded positive-sense RNA virus whose genome contains three open reading frames (ORF1, ORF2, and ORF3), 5′ and 3′ untranslated regions (UTRs), and a poly(A) tract at the 3′ end. (nature.com)
Here, using the dRNA-seq technique in combination with ANNOgesic analysis, we successfully mapped and annotated transcription start sites (TSS) of both E. faecalis V583 and E. faecium AUS0004 at single nucleotide resolution. (frontiersin.org)
Genomic variations in a population can arise due to single nucleotide changes in the DNA or structural changes to chromosomes. (jove.com)
These variations can be a single nucleotide or a larger chromosomal variation. (jove.com)
The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1% of the population. (jove.com)
Our findings indicated that TT, as the two-single-nucleotide polymorphism haplotype in promoter region of CYP11B2 gene, is associated with plasma aldosterone concentration in the southern population of Iran. (ephysician.ir)
Each SNP represents a difference in a single DNA building block , called a nucleotide. (medlineplus.gov)
An extensive expression marker map was created and additional single nucleotide polymorphism markers were developed. (usda.gov)
This process helps identify single or multiple nucleotide variations as well as areas of insertion or deletion. (msdmanuals.com)
Identification of oil palm cis-regulatory elements based on DNA free energy and single nucleotide polymorphism density. (bvsalud.org)
using the combination of DNA free energy and single nucleotide polymorphism (SNP) density approaches. (bvsalud.org)
Multiple distinct single- and double-nucleotide substitution signatures were discernible. (lu.se)
Polymorphisms1
In addition, we determined polymorphisms in the promoter and all coding regions of FCN2 in 40 Gabonese. (nih.gov)
Genome6
The initial PCR reaction amplifies a region of the HCV genome (External primers). (cdc.gov)
Partial sequencing of the virus capsid protein coding region (VP1) of the poliovirus genome at the MDH laboratory identified it as a vaccine-derived poliovirus (VDPV). (cdc.gov)
Additional sequencing of the entire poliovirus genome at the CDC polio laboratory confirmed that this strain was a VDPV, with 2.3% divergence in the VP1 region from the parent Sabin type 1 strain. (cdc.gov)
The method works very well for most of the genome, but, as previously noted, some regions are left out. (jax.org)
Mutations in other regions of the genome may also be informative for known VOI/VOC detection. (who.int)
CoV-2 genome and can be targeted as a region for detection of VOC/VOI. (who.int)
Protein2
Nucleotide excision repair relies on specific protein complexes to recognize damaged regions of DNA and flag them for removal and repair. (jove.com)
Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
Genomic2
It can be accessed online at www.helmholtz-hiri.de/en/datasets/enterococcus through an instance of the genomic viewer JBrowse. (frontiersin.org)
DNA polymerase fills the gap with new nucleotides, and then the DNA ligase enzyme seals the edges between the new and old DNA. (jove.com)
Genes4
We built a set T of flanking regions of genes with 1-3 introns from 21st and 22nd chromosomes extracted from GenBank to define positions having conserved properties, namely hydrophobicity, that are potentially essential for recognition by spliceosome. (ijbs.com)
The aim of the current study was to extend these structural findings and to examine links between putative RD-genes and functional connectivity of language-related regions in healthy controls (n = 27) and schizophrenia (n = 28). (nih.gov)
Thus, RD-genes influence functional connectivity in language-related regions, but no RD-gene uniquely affected network function in schizophrenia as compared to controls. (nih.gov)
A region of 10 genes or less was deemed an acceptable gap between anchors, and thus variability in the gene order was allowed. (ubc.ca)
Substitutions2
therefore, we sequenced the coding region of NAT2 in 456 chromosomes from 13 populations from the Americas, and two from Siberia, detecting nine substitutions and 11 haplotypes. (unibo.it)
Base substitutions in these regions were almost exclusively of cytosine at TpC dinucleotides. (lu.se)
Polio2
Polioviruses are isolated in polio laboratories from faecal samples of acute flaccid paralysis cases The Eastern Mediterranean Region Polio Laboratory Network was established in 1991 by WHO and national governments. (who.int)
The Eastern Mediterranean Region Polio Laboratory Network is subject regularly to accreditation visits and all the network laboratories are accredited. (who.int)
Cytosine1
For example, a SNP may replace the nucleotide cytosine (C) with the nucleotide thymine (T) in a certain stretch of DNA. (medlineplus.gov)
Genetic1
While associations between individual human brain regions and environmental or genetic factors have been investigated, how brain age is associated with those factors remains unclear. (nature.com)
Chromosomal1
Biparental and association mapping were used to locate chromosomal regions for dryland and drought adaptation. (usda.gov)
Gene4
5'-untranslated region (5'UTR), the hypervariable region 1 (HVR1), non-structural gene 5a (NS5a) and non-structural gene 5b (NS5b). (cdc.gov)
In European-American and Asian regions where starch-based diets are common, the population shows a higher copy number of gene AMY1, a gene which is involved in starch metabolism. (jove.com)
As a proangiogenic response effecter, the interaction of Sema4D with plexin-B1 is dependent on the COOH-terminal PDZ-binding motif of plexin-B1, which binds two guanine nucleotide exchange factors (GEFs) for Rho and is mediated by the activation of Rho-initiated (Ras homolog gene family) pathways ( 4 - 8 ). (jcancer.org)
Attenuated huntingtin gene CAG nucleotide repeat size in individuals with Lynch syndrome. (lu.se)
Assay1
Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
Sabin1
All isolates differed from the Sabin type 2 reference strain by 6-13 nucleotide changes, and all were detected in the National Capital Region and Region 4, which had conducted supplementary immunization activities. (who.int)
Viruses2
All wild viruses are subjected to nucleotide sequencing to monitor the virus transmission pattern and relationships. (who.int)
Map 1: the map describes national viruses isolation, intratypic differentiation and nucleotide sequencing laboratories. (who.int)
Isolates1
partial sequencing of the VP1 region of these three isolates has indicated they also are VDPV type 1. (cdc.gov)
Orthologs1
Un-ambiguous orthologs were identified and synteny blocks anchored to these regions. (ubc.ca)
Polymerase1
Hepatitis C RNA: Testing for HCV RNA by reverse-transcriptase ¬polymerase-chain reaction (RT-PCR) amplification of the 5' noncoding region was performed on anti-HCV positive samples. (cdc.gov)
Population2
Generally, only a nucleotide variation that is found in more than 1% of the population is referred to as SNP. (jove.com)
Analysis of nucleotide diversity of NAT2 coding region reveals homogeneity across Native American populations and high intra-population diversity. (unibo.it)
Mutations2
Furthermore, we developed a targeted ultra-deep sequencing approach to assess the dynamics of clinically relevant mutations in the RdRp-region and to detect insertions in the HVR-domain in the HEV genomes. (nature.com)
Amino acid mutations of the core region (Nos. (wjgnet.com)
Change2
[ 13 ] About 98% of diagnosed patients have the G1138A mutation, resulting in a G-to-A DNA nucleotide point change. (medscape.com)
One percent of cases have a G-to-C DNA point change at nucleotide 1138, causing the G1138C mutation. (medscape.com)
Distinct1
As a consequence, we suggest that elements of these distinct regions will likely be responsible for the hairiness or uniform coat of hair that is so distinct. (ubc.ca)
Approaches1
Bioinformatics approaches outlined a low nucleotide identity of P. parmentieri and P. wasabiae with other species, while P. carotovorum subsp. (mdpi.com)
Proteins2
NER employs a set of specialized proteins that first scan DNA to detect a damaged region. (jove.com)
Intracellular signaling proteins that are defined by the presence of a NUCLEOTIDE-binding region and LEUCINE-rich repeats. (bvsalud.org)
Primers2
Region-specific primers are attached to a universal tail and contain a blocker at the 3 -end to prevent extension. (ojp.gov)
During RC-PCR, functional region-specific, tailed index PCR primers are generated and extended, followed by multiplex amplification of the target regions. (ojp.gov)
Human1
Using this new approach, we not only identified several insertions of human (AHNAK, RPL18) and viral origin (RdRp-derived) in the HVR-region isolated from an exemplary sample but detected a variant containing two different insertions simultaneously (AHNAK- and RdRp-derived). (nature.com)
Specific2
Using our new profiles based on hydrophobicity properties we pointed out specific properties for regions surrounding splice sites. (ijbs.com)
It is known that certain lifestyle habits are associated with accelerated atrophy in specific brain regions. (nature.com)
Pattern2
Highly repetitive segments, where the same nucleotide or pattern (e.g. (jax.org)
Anti-correlation pattern between free energy and SNP density was observed on the predicted regions of CREs. (bvsalud.org)
Frequently1
There were three nucleotide variants (T471C/A600G/C862T) frequently detected in cancer cell lines. (jcancer.org)
Brain1
To date, it is still unclear how smoking and alcohol consumption is associated with brain structural aging, especially when the morphology of all the brain regions is considered. (nature.com)
Central3
The binding site for PDE α and β subunits are likely to be in the central region of the PDE γ subunits. (wikipedia.org)
Su estructura general consta de una cualquiera de las variedades de dominios efectores en su N-terminal, como el dominio de reclutamiento de caspasa (CARD), el dominio central de unión a nucleótidos, y un número variable de repeticiones ricas en leucina C-terminales. (bvsalud.org)
Their general structure consists of any of a variety of effector domains at their N-termini such as a caspase recruitment domain (CARD), a central nucleotide-binding domain, and a variable number of C-terminal leucine-rich repeats. (bvsalud.org)
Virus2
Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)
West African sub-region with consideration of the mented RNA virus belonging to the Arenaviridae origin of the virus, its properties/strains, epidemiol- family. (folkhalsomyndigheten.se)