Nucleotide caenorhabditis elegans proteins. Medical search. Frequent questions

Using a forward genetic screen, we identified two alleles of smc-5 that exacerbate UV sensitivity in Caenorhabditis elegans. (uni-koeln.de)

The structure of the nervous system of the nematode Caenorhabditis elegans. (academicinfluence.com)
Nondisjunction Mutants of the Nematode CAENORHABDITIS ELEGANS. (academicinfluence.com)
Toward a physical map of the genome of the nematode Caenorhabditis elegans. (academicinfluence.com)
We found that between 5 and 10% of all L. stagnalis maternal transcripts (~300-400 genes) are also present in the equivalent arthropod (Drosophila melanogaster), nematode (Caenorhabditis elegans), urochordate (Ciona intestinalis) and chordate (Homo sapiens, Mus musculus, Danio rerio) datasets. (nottingham.ac.uk)
Its excellent performance was proved in an objective competition based on the genome of the nematode Caenorhabditis elegans. (plob.org)

The repeat is found in a variety of proteins that include, vitellogenin receptor from Drosophila melanogaster, low-density lipoprotein (LDL) receptor [ ( PUBMED:6091915 ) ], preproepidermal growth factor, and nidogen (entactin). (embl.de)
At the Drosophila neuromuscular junction (NMJ), the small GTPase Rab3 controls the distribution of CAZ proteins across release sites, thereby regulating the efficacy of individual AZs. (eneuro.org)
As the Drosophila homologue of mammalian DENN/MADD and Caenorhabditis elegans AEX-3, Rab3-GEF is a guanine nucleotide exchange factor (GEF) for Rab3 that stimulates GDP to GTP exchange. (eneuro.org)
We show here that Rab3-GEF is required for controlling the protein composition of release sites in the Drosophila neuromuscular junction (NMJ). (eneuro.org)
Caenorhabditis elegans , Drosophila melanogaster , Homo sapiens , Arabidopsis thaliana , Saccharomyces cerevisiae , Schizosaccharomyces pombe and Encephalitozoon cuniculi . (biomedcentral.com)

An examination revealed the presence of two serpin genes, each coding for two or three likely alternative reactive centre exon cassettes, respectively, also in the Caenorhabditis elegans genome, The occurrence of such serpin genes in some groups of metazoa reflects a parsimonious way to enlarge the adaptive ability of these organisms to cope with a plethora of different serine and cysteine proteases. (uni-bielefeld.de)
More than half of all human genes are alternatively spliced, so it is expected that alternative pre- mRNA splicing will be responsible for the identification of more than 90 percent of all human proteins. (genomicglossaries.com)
Alternative splicing is the primary mechanism by which a limited number of protein-coding genes can generate proteome diversity. (biologists.com)
As the genomes of different organisms are sequenced and annotated it is becoming apparent that the complexity of an organism does not depend on the total number of protein-coding genes. (biologists.com)
While the majority of these conserved maternal transcripts ("COMATs") have housekeeping gene functions, they are a non-random subset of all housekeeping genes, with an overrepresentation of functions associated with nucleotide binding, protein degradation and activities associated with the cell cycle. (nottingham.ac.uk)
This facilitates large-scale experiments by making it possible to tag hundreds of genes with fluorescent proteins. (phys.org)
Finally, the group modeling diseases in C. elegans, directed by Dr. Cerón at IDIBELL, intends to use nested CRISPR to replace genes in C. elegans with their human counterparts. (phys.org)
mGene is a computational tool for the genome-wide prediction of protein coding genes from eukaryotic DNA sequences. (plob.org)
The evaluated developmental version of mGene exhibited the best prediction performance (in terms of the average between sensitivity and specificity) for the multiple-genome prediction tasks on all four evaluation levels (considering, nucleotides, exons, transcripts and genes). (plob.org)

Aside from binding to membranes, the activated FERM domain of ERM proteins can also bind the guanine nucleotide dissociation inhibitor of Rho GTPase (RhoDGI), which suggests that in addition to functioning as a cross-linker, ERM proteins may influence Rho signalling pathways. (embl.de)
The active conformation is promoted by guanine-nucleotide exchange factors, and the inactive state by GTPase-activating proteins (GAPs) which stimulate the intrinsic GTPase activity of small G proteins. (embl-heidelberg.de)

We were able to recover known consensus nucleotide sequences for 12 RBPs and discovered novel binding preferences for 3 of the RBPs namely, Scp160p, Sik1p and Tdh3p. (columbia.edu)
In the early 1990s, molecular biologist Douglas Prasher, at the Marine Biology Laboratory, used GFP to design probes, a technology involving fragments of DNA to detect the presence of nucleotide sequences. (asu.edu)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

The data for these analyses comes from segregating populations of Saccharomyces cerevisiae (baker's yeast) as well as Caenorhabditis elegans (roundworm). (columbia.edu)
The National Human Genome Research Institute (NHGRI) has supported a number of sequencing projects aimed at producing draft assemblies of roundworm (Caenorhabditis) genomes. (genome.gov)
CB5161 -- and subsequent analyses to compare the resulting data to the existing roundworm genomes should allow for more thorough annotation of the C. elegans genome. (genome.gov)
He established the roundworm Caenorhabditis elegans as a model organism for the investigation of developmental biology, and founded the Molecular Sciences Institute in Berkeley, California, United States. (academicinfluence.com)

Recently, improved bioinformatics applied to Argonaute HITS-CLIP enables identification of binding sites with single nucleotide resolution. (wikipedia.org)
It has since been found to be a bona-fide tumor suppressor, but its exact function remained elusive until, in 2018, a series of single nucleotide polymorphisms were identified as the reason for a striking breath malodor of patients in a Dutch hospital. (olsztyn.pl)

HITS-CLIP of the RNA-binding protein Argonaute has been performed for the identification of microRNA targets by decoding microRNA-mRNA and protein-RNA interaction maps in mouse brain, and subsequently in Caenorhabditis elegans, embryonic stem cells and tissue culture cells. (wikipedia.org)
When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)
MicroRNAs (miRNAs) are short non-coding RNAs, typically 21-23 nucleotides long, that function in post-transcriptional gene regulation typically through translation inhibition and/or mRNA degradation ( 1 ). (amegroups.org)
Fertilization also leads to changes in mRNA translation and protein stability, which support a period of development driven off of maternal stockpiles. (elifesciences.org)

We show that the Caenorhabditis elegans egl-2 gene encodes an eag K + channel and that a gain-of-function mutation in egl-2 blocks excitation in neurons and muscles by causing the channel to open at inappropriately negative voltages. (jneurosci.org)
The genome is approximately 97Mb in size, and encodes over 19,000 proteins. (genome.gov)
Oligophrenin-1 encodes a rhoGAP protein involved in X-linked mental retardation. (embl-heidelberg.de)
This gene is highly expressed in fetal brain and encodes a protein of relative molecular mass 91K, named oligophrenin-1, which contains a domain typical of a Rho-GTPase-activating protein (rhoGAP). (embl-heidelberg.de)

The powerful genetics of C. elegans will allow me to study the cellular pathways that contribute to that spreading of these toxic molecules. (uw.edu)
The genetics of Caenorhabditis elegans. (academicinfluence.com)
Jeremy Vicencio et al, Efficient Generation of Endogenous Fluorescent Reporters by Nested CRISPR in Caenorhabditis elegans, Genetics (2019). (phys.org)

The amino-acid sequence of the FERM domain is highly conserved among ERM proteins and is responsible for membrane association by direct binding to the cytoplasmic domain or tail of integral membrane proteins. (embl.de)
For cytoskeleton-membrane cross-linking, the dormant molecules becomes activated and the FERM domain attaches to the membrane by binding specific membrane proteins, while the last 34 residues of the tail bind actin filaments. (embl.de)
Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human neurons. (jove.com)

Rapid GTPase activating protein (GAP)-mediated inactivation (RhoA flux) is proposed to limit RhoA zone dimensions. (cipsm.de)
GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. (embl-heidelberg.de)
By enhancing their GTPase activity, GAP proteins inactivate small Rho and Ras proteins, so inactivation of rhoGAP proteins might cause constitutive activation of their GTPase targets. (embl-heidelberg.de)
In particular, we propose that Arg 85 and Asn 194 are involved in binding G proteins and enhancing GTPase activity. (embl-heidelberg.de)
These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. (senescence.info)

Here we identify Rab3-GEF as a second protein that acts in conjunction with Rab3 to control AZ protein composition. (eneuro.org)
Our results indicate that loss of Rab3-GEF results in the development of NMJs that are structurally and functionally indistinguishable from NMJs that lack the protein Rab3. (eneuro.org)
Our studies reveal that Rab3 and Rab3-GEF act within the same molecular mechanism and support a model wherein Rab3-GEF serves as a Rab3 effector to control active zone protein composition. (eneuro.org)

In contrast, the DNA damage accumulation and sensitivity of smc-5 mutant strains to replication impediments are suppressed by mutations in the C. elegans BRCA1/BARD1 homologs, brc-1 and brd-1. (uni-koeln.de)
In previous work, we found that gain-of-function mutations that hyperactivate GEM-1 (an SLC16A transporter protein) can bypass the requirement for GON-2 (a TRPM channel protein) during the initiation of gonadogenesis in C. elegans. (cipsm.de)
However, even though it allows the creation or correction of mutations consisting of a single or few nucleotides with relative ease, it still possesses limitations for larger fragments of DNA in the genome. (phys.org)

This paper claims to identify a long-sought ligand for the receptor protein tyrosine phosphatase Lar that mediates its functions in neuromuscular junction development, mushroom body development, and photoreceptor axon targeting. (sciety.org)
Receptor-like protein-tyrosine phosphatases (RPTPs) have cytoplasmic phosphatase domains and cell adhesion molecule (CAM)-like extracellular domains that interact with cell-surface ligands and/or co-receptors. (sciety.org)
use an assay in which alkaline phosphatase-tagged cell surface proteins are used to stain live embryos overexpressing other cell surface proteins to identify Sticks and stones (Sns) as a possible ligand for the Lar receptor protein tyrosine phosphatase. (sciety.org)
Caenorhabditis elegans protein phosphatase ptp-1. (embl.de)

Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
For example, the group is currently working on the tagging of these fluorescent proteins with peptides that allow their degradation in a controlled manner. (phys.org)

Homo sapiens OPRTase and ODCase activities lower to a greater extent when heated than the fused protein does. (wikipedia.org)

The deduced 4660-aa sequence, expected to constitute a mature protein of M(r) 516,715, consists of a probable N-terminal signal peptide sequence (25 aa), an extracellular region (4400 aa), a single transmembrane domain (22 aa), and a C-terminal cytoplasmic tail (213 aa). (embl.de)
We can use Caenorhabditis Elegans as the model system because the nematode secretes the ECVs outside into the extracellular environment. (uw.edu)
I have found that transgenic nematodes (Caenorhabditis elegans) that express human versions of the toxic proteins characteristic of Alzheimer's disease (Amyloid beta) are transported by extracellular vesicles (ECVs). (uw.edu)

This presentation will summarize the work on SELENBP1 performed in the Klotz lab regarding the enzymatic activity of SELENBP1, the role of selenium, the role of transition metal ions and the search for a function of this protein, using isolated SELENBP1 mutants, cell culture models and a model organism, Caenorhabditis elegans. (olsztyn.pl)
The genome of C. elegans was the first of any multicellular organism to be sequenced in its entirety. (genome.gov)
The group of Dr. Cerón at the Bellvitge Biomedical Research Institute (IDIBELL) used the model organism Caenorhabditis elegans to optimize the technique, leading to the development of a method called nested CRISPR. (phys.org)

Non-receptor tyrosine-protein kinase TYK2. (embl.de)

green fluorescent protein (GFP) fusions revealed egl-2 expression in a subset of neurons and muscles that could explain the mutant behavioral defects. (jneurosci.org)
Caenorhabditis elegans senses and navigates its complex chemical environment using a small subset of sensory neurons [ 22 - 24 ]. (plos.org)

The ab-initio version was best on nucleotide, exon and transcript level, and only slightly worse than Augustus on the gene level. (plob.org)

PAR-CLIP, for identifying the binding sites of cellular RNA-binding proteins (RBPs) and microRNA-containing ribonucleoprotein complexes (miRNPs) in tissue culture cells. (wikipedia.org)
The first study focused on inferring the in vitro binding specificity of RNA-binding proteins (RBPs). (columbia.edu)
The third study focused on post-transcriptional regulatory networks in yeast, by mapping the regulatory activity level of RNA binding proteins (RBPs) as a quantitative trait in so-called "aQTL" analysis. (columbia.edu)

The conservation of functionalities involved in nucleotide binding and catalysis also provide insights into the mechanistic features of members of the family of actin-related proteins. (rcsb.org)

Certain species-specific SSR motifs were identified, along with a hexa-nucleotide motif shared only with the other two Compositae species (sunflower (Helianthus annuus) and horseweed (Conyza canadensis)) included in the study. (researchgate.net)
The approximately 40% of KOGs that are represented in six or seven species are enriched in proteins responsible for housekeeping functions, particularly translation and RNA processing. (biomedcentral.com)
Proteins found in any species of insect. (lookformedical.com)

Photoreceptor cyclic nucleotide-gated (CNG) channels regulate Ca2+ influx in rod and cone photoreceptors. (cipsm.de)
Proteins that regulate vesicle exocytosis cluster at AZs, composing the cytomatrix at the active zone (CAZ). (eneuro.org)

We present evidence that the Snail-related transcription factor CES-1 of Caenorhabditis elegans coordinates these processes in a specific cell lineage. (cipsm.de)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)

These transcripts may code for eight different Sp4 protein isoforms with different biological functions, which dependent on the splice pattern - either may be secreted. (uni-bielefeld.de)

Filopodin, a slime mold protein that binds actin and which is involved in the control of cell motility and chemotaxis. (embl.de)
ERM proteins are made of three domains, the FERM domain, a central helical domain and a C-terminal tail domain, which binds F-actin. (embl.de)
This work highlights both structural and mechanistic similarities with the small and trimeric G proteins and restricts the types of mechanisms responsible for the considerable enhancement of ATP hydrolysis associated with actin polymerization. (rcsb.org)
Members of the Rho family of small G proteins transduce signals from plasma-membrane receptors and control cell adhesion, motility and shape by actin cytoskeleton formation. (embl-heidelberg.de)

In microorganisms, these two domains are separate proteins, but, in multicellular eukaryotes, the two catalytic sites are expressed on a single protein, uridine monophosphate synthase. (wikipedia.org)

The test-tube system confirmed previous suggestions that a protein called Puf3 forms a bridge between Ccr4-Not and mRNAs. (elifesciences.org)
Spermatogenesis produces haploid sperm capable of penetrating the oocyte, whereas oogenesis produces differentiated oocytes that are stockpiled with maternal nutrients, proteins, and mRNAs, and have outer layers that protect the embryo and enable fertilization. (elifesciences.org)

Like all other GTPases, Rho proteins act as molecular switches, with an active GTP-bound form and an inactive GDP-bound form. (embl-heidelberg.de)

High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) is a variant of CLIP for genome-wide mapping protein-RNA binding sites or RNA modification sites in vivo. (wikipedia.org)

Since their discovery in Caenorhabditis elegans , miRNAs have been extensively investigated as master regulators of gene expression in a variety of animal, plant, and human models. (amegroups.org)

The structural maintenance of chromosomes (SMC) proteins form distinct complexes that maintain genome stability during chromosome segregation, homologous recombination, and DNA replication. (uni-koeln.de)

Focal-adhesion kinases (FAKs), cytoplasmic protein tyrosine kinases involved in signalling through integrins. (embl.de)

The backbone dynamics of the amyloid precursor protein (APP) transmembrane helix was compared to those of other transmembrane domains. (cipsm.de)

Here we report that the behavioral defects in the C. elegans egl-2 mutant are caused by a gain-of-function ( gf ) mutation in an eag-like K + channel. (jneurosci.org)
Here, we show that the behavioral response of Caenorhabditis elegans to bacterially produced medium-chain alcohols switches from attraction to avoidance when presented in the background of a subset of additional attractive chemicals. (plos.org)

FERM domains are found in a number of cytoskeletal-associated proteins that associate with various proteins at the interface between the plasma membrane and the cytoskeleton. (embl.de)
This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. (senescence.info)

Cells cope with UV-induced DNA damage through two general strategies to repair the damaged nucleotides and to promote cell cycle progression in the presence of UV-damaged DNA. (uni-koeln.de)
Talin, a cytoskeletal protein concentrated in regions of cell-substratum contact and, in lymphocytes, of cell-cell contacts. (embl.de)
Using DNA recombinant technology, scientists combine the Gfp gene to a another gene that produces a protein that they want to study, and then they insert the complex into a cell. (asu.edu)

The SAD1/RAD53 protein kinase controls multiple checkpoints and DNA damage-induced transcription in yeast. (academicinfluence.com)

Protein-tyrosine phosphatases PTPN3 and PTPN4, enzyme that appear to act at junctions between the membrane and the cytoskeleton. (embl.de)
Protein-tyrosine phosphatases PTPN14 and PTP-D1, PTP-RL10 and PTP2E. (embl.de)

For instance, the genomic insertion of a gene that produces a fluorescent protein such as the widely-used GFP suffers from poor efficiency and involves complicated cloning steps. (phys.org)

A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)

Green fluorescent protein (GFP) is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light. (asu.edu)
The protein has 238 amino acids, three of them (Numbers 65 to 67) form a structure that emits visible green fluorescent light. (asu.edu)
Gfp refers to the gene that produces green fluorescent protein. (asu.edu)
John W. Hasting and James G. Morin, who later researched aequorin, termed the protein as green fluorescent protein in 1971. (asu.edu)
Moreover, since nested CRISPR is modular, additional peptides or proteins of interest can also be integrated in combination with these fluorescent proteins. (phys.org)

Direct binding between the two proteins was confirmed in vitro, although it requires extensive multimerization of Lar, and is conserved between PTPRF/D and Nephrin homologues. (sciety.org)

From 1979 to 1992, many researchers studied various aspects of GFP, including the use of Nuclear Magnetic Resonance to study the amino acids of the protein, the use of X-rays to study its crystal, and the evolution of GFP. (asu.edu)

Nearly all these proteins are subunits of known or predicted multiprotein complexes, in agreement with the balance hypothesis of evolution of gene copy number. (biomedcentral.com)

Model organisms with a short life cycle such as C. elegans enable researchers to explore both the possibilities and limitations of CRISPR. (phys.org)

The raw wash is then run through a size exclusion column to separate the ECVs, and the different size samples are either run through a BCA Protein Assay to determine amount of protein in each sample based on an absorbance reading, or run through an SDS-PAGE Protein Gel and treated with antibodies to isolate the aβ protein. (uw.edu)

Selenium-binding protein 1 (SELENBP1) was identified some thirty years ago as a selenium-containing protein that does not count among the 25 known human selenoproteins harboring selenocysteine residues. (olsztyn.pl)

Anatomy10

Organisms6

Diseases1

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Psychiatry and Psychology1

Phenomena and Processes36

Information Science4

Health Care1

Sensitivity in Caenorhabditis elegans1

Nematode Caenorhabditis elegans5

Drosophila5

Genes9

Guanine2

Sequences4

Roundworm4

Single-nucleotide2

MRNA8

Encodes4

Genetics3

Membrane proteins3

GTPase5

Rab33

Mutations3

Phosphatase4

Degradation2

Homo1

Extracellular3

Organism3

Receptor1

Neurons2

Exon1

RBPs3

Catalysis1

Species3

Regulate2

Processes1

Specificity1

Transcripts1

Actin4

Multicellular1

MRNAs2

Molecular1

Vivo1

Extensively1

Stability1

Cytoplasmic1

Amyloid1

Behavioral2

Plasma membrane2

Cell3

Kinase1

Tyrosine2

Genomic1

Sequence1

Fluorescent5

Vitro1

Amino1

Complexes1

Model1

Assay1

Human1