• Presumably, such biased nucleosome occupancy facilitates the origination of new exons by increasing the splice strength of the ancestral nonexonic regions through driving a local difference in GC content. (pnas.org)
  • Our partner EpiCypher has developed the largest portfolio of fully defined and homogenous purified and recombinant nucleosomes on the market incorporating different histone modifications, site mutations and variants, see links above. (biocat.com)
  • In April 2015, he founded Nucleosantos Therapeutics, a start-up that is working on the discovery and development of exogenous Nucleosome-Binding Molecules. (omicsonline.org)
  • He discovered the 3-bp and 10-bp periodicity in the DNA sequences, as well as the rules determining the curvature of DNA molecules and their bending within nucleosomes. (wikipedia.org)
  • Thus, association of DNA with nucleosomes may not necessarily preclude template activity, although such association is probably of a dynamic rather than a static nature. (sciencemag.org)
  • Recent structural analyses using in vitro -reconstituted model nucleosome arrays based on the strong 601 DNA nucleosome positioning sequence ( 6 ) have led to the proposal of two models for the 30-nm chromatin fiber that differ in topology, dimension, and nucleosome packing density. (pnas.org)
  • The second model was derived from tight constraints obtained from measurements of the physical dimensions of long nucleosome arrays visualized by EM ( 8 ). (pnas.org)
  • The key difference between the two 30-nm chromatin fiber models is that the interdigitated model was derived from nucleosome arrays saturated with linker histone, whereas the two-start helix model was derived from a tetra-nucleosome core array in the absence of linker histone. (pnas.org)
  • Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). (wikipedia.org)
  • The nucleosome hypothesis was proposed by Don and Ada Olins in 1974 and Roger Kornberg. (phys.org)
  • Chromatin organization is central to many conserved biological processes, but it is generally unknown how the underlying nucleosomes are arranged in situ. (springer.com)
  • The other is known as the "bulge" or "loop-recapture" mechanism and it involves the dissociation of DNA at the edge of the nucleosome with reassociation of DNA inside the nucleosome, forming a DNA bulge on the octamer surface. (wikipedia.org)
  • Mice that have T cells directed towards the nucleosome can elicit a response to other antigens such as dsDNA and histone via a mechanism known as antigen spreading. (wikipedia.org)
  • It is also possible that certain DNA elements could regulate remodeling by inhibiting the interaction of nucleosomes with the remodeling enzyme. (pubmedcentralcanada.ca)
  • Nucleosome placement, usually measured by quantifying protection of DNA from enzymatic digestion, can regulate accessibility. (nature.com)
  • Arriving from elsewhere - very likely from another nearby nucleosome - the DNA meets the "spool" at upper right, wraps around the back of it, circles diagonally downward across the front face, and then around the back again, exiting at lower left toward a third (also not shown) nucleosome. (natureinstitute.org)
  • Remarkably, the same is not true of zinc-finger nucleases (ZFNs), which cleave equally well at nucleosome-occupied and nucleosome-depleted sites. (pnas.org)