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  • vitro
  • This kit allows the quantitation of apoptotic cells in vitro by DNA affinity-mediated capture of free nucleosomes followed by their anti-histone-facilitated detection. (bio-medicine.org)
  • We propose that different reconstitution protocols in vitro alter the energy landscape for nucleosome binding and so alter positional preference. (ens-lyon.fr)
  • spool
  • Arriving from elsewhere - very likely from another nearby nucleosome - the DNA meets the "spool" at upper right, wraps around the back of it, circles diagonally downward across the front face, and then around the back again, exiting at lower left toward a third (also not shown) nucleosome. (natureinstitute.org)
  • structural
  • Scientists do not completely understand how nucleosomes manage to remain stable and labile at the same time, according to the Research Collaboratory for Structural Bioinformatics. (reference.com)
  • core
  • Is there an influence of DNA or the nucleosome core on the secondary structure of histone tails? (dkfz.de)
  • 1 ) with ∼100 bp of the rice CentO tandem repeat sequence (red) folding once around the nucleosome core that includes CenH3 (yellow). (pnas.org)
  • Nucleosomes are the basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound around a histone protein core. (phys.org)
  • The first model was constructed by using the crystal structure at 9 Å of a tetra-nucleosome core array and is of a two-start helix type ( 7 ). (pnas.org)
  • Luger K, Mader AW, Richmond RK, Sargent DF, Richmond TJ: Crystal structure of the nucleosome core particle at 2.8 A resolution. (springer.com)
  • Davey CA, Sargent DF, Luger K, Maeder AW, Richmond TJ: Solvent mediated interactions in the structure of the nucleosome core particle at 1.9 a resolution. (springer.com)
  • The box represents the promoter, and dots indicate occupied nucleosome positions, with nucleosome positions N-1 (the core promoter), N-2, and N-3 at the top, middle, and bottom, respectively. (nih.gov)
  • exonic
  • By identifying recently evolved exons in human but not in macaque using matched RNA sequencing, we found that higher exonic nucleosome occupancy also existed in macaque regions orthologous to these exons. (pnas.org)
  • Notably, previous studies in mammalian cell lines, or model organisms like Caenorhabditis elegans and Drosophila melanogaster , have found relatively higher levels of nucleosome occupancy at exonic regions than at intronic regions ( 7 ⇓ ⇓ ⇓ - 11 ). (pnas.org)
  • structures
  • After all, we have yet to encounter any structures in the cell nucleus that are not preeminently functioning, dynamic forms , and the nucleosome proves no different. (natureinstitute.org)
  • explored seven structures of the RNAPII-nucleosome complex, in which RNAPII pauses at four locations on the nucleosome. (sciencemag.org)
  • Here we report the cryo-electron microscopy structures of RNAPII-nucleosome complexes in which RNAPII pauses at the superhelical locations SHL(−6), SHL(−5), SHL(−2), and SHL(−1) of the nucleosome. (sciencemag.org)
  • facilitates
  • It has been speculated that HELLS, which belongs to the SNF2 family ATPase, facilitates DNA methylation by DNMT3b through nucleosome remodeling, but HELLS on its own fails to exhibit such an activity. (pnas.org)
  • arrays
  • The second model was derived from tight constraints obtained from measurements of the physical dimensions of long nucleosome arrays visualized by EM ( 8 ). (pnas.org)
  • reveals
  • Brower-Toland BD, Smith CL, Yeh RC, Lis JT, Peterson CL, Wang MD (2002) Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA. (springer.com)