Nucleoside monophosphate kinase. Medical search. Frequent questions

Abstract Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of malignancy. (nanoker-society.org)
Introduction Uridine-cytidine kinase 2 (UCK2) is an enzyme that catalyses the conversion of uridine and cytidine to their monophosphate form of uridine and cytidine in an alternative salvage pathway of pyrimidine biosynthesis [1]. (nanoker-society.org)
A pyrimidine nucleoside is one in which the nucleobase is a pyrimidine, such as cytosine in cytidine. (biologyonline.com)
Cytidine is a nucleoside consisting of cytosine and ribose sugar linked by β-N 1 -glycosidic bond. (biologyonline.com)
Nucleosides such as cytidine can be produced by de novo synthesis pathways in the liver. (biologyonline.com)
Cytidine, just as the other nucleosides, can give rise to nucleotides. (biologyonline.com)
Cytidine can form cytidine monophosphate (CMP, i.e. cytidine with a single phosphate group), cytidine diphosphate (CDP, i.e. cytidine with two phosphate groups), and cytidine triphosphate (CTP, i.e. cytidine with three phosphate groups). (biologyonline.com)
KP-1461 , a cytidine analogue belonging to the class of nucleoside reverse transcriptase inhibitors, has a potential use as a treatment for HIV/AIDS. (biologyonline.com)
Fricke J, Neuhard J, Kelln RA, Pedersen S: The cmk gene encoding cytidine monophosphate kinase is located in the rpsA operon and is required for normal replication rate in Escherichia coli. (drugbank.com)
Myeloid-specific ablation of LPS-induced cytidine monophosphate kinase 2 (CMPK2), which is rate limiting for mtDNA synthesis, reduced ARDS severity without a direct effect on IL-6. (lu.se)

Purines, such as adenine and guanine nucleotides, are derived from the nucleotide inosine monophosphate (IMP) since purines are synthesized as ribonucleotides and not as free nucleobases (as opposed to pyrimidines that are synthesized first as a free base). (biologyonline.com)
These mechanisms were developed using structurally conserved amino acid residues within hydrogen-bonding distance of a nucleotide in the active sites of crystallised kinases of a particular mechanistic class. (biomedcentral.com)
Our data collectively demonstrated that carboxylesterase 1 (CES1) and cathepsin A (CatA) are enzymes involved in hydrolyzing RDV to its alanine intermediate Met X, which is further hydrolyzed to the monophosphate form by histidine triad nucleotide-binding protein 1 (HINT1). (inrae.fr)
Histidine triad nucleotide-binding protein 1 (Hint 1) then removes the amino acid moiety, which is followed by hydrolysis of the methoxyl group at the O(6)-position of the guanine base by adenosine deaminase-like protein 1 (ADAL1) to give 2'-deoxy-2'-fluoro-2'-C-methylguanosine-5'-monophosphate. (inrae.fr)
When phosphorylated by kinases, the nucleoside is converted into a nucleotide. (biologyonline.com)
Thus, a nucleotide is a nucleoside with a phosphate group. (biologyonline.com)
Differences in expression profiles, substrate specificities, kinetic properties and subcellular localization among the AK (adenylate kinase) isoenzymes have been shown to be important for maintaining a proper adenine nucleotide composition for many different cell functions. (rhea-db.org)
Briozzo P, Golinelli-Pimpaneau B, Gilles AM, Gaucher JF, Burlacu-Miron S, Sakamoto H, Janin J, Barzu O: Structures of escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity. (drugbank.com)

Nucleoside diphosphate kinase is the primary enzyme involved in phosphorylation of the diphosphate to the active triphosphate, PSI-352666. (inrae.fr)

In enzymology, a nucleoside-phosphate kinase (EC 2.7.4.4) is an enzyme that catalyzes the chemical reaction ATP + nucleoside phosphate ⇌ {\displaystyle \rightleftharpoons } ADP + nucleoside diphosphate Thus, the two substrates of this enzyme are ATP and nucleoside monophosphate, whereas its two products are ADP and nucleoside diphosphate. (wikipedia.org)
The systematic name of this enzyme class is ATP:nucleoside-phosphate phosphotransferase. (wikipedia.org)
This enzyme is also called NMP-kinase, or nucleoside-monophosphate kinase. (wikipedia.org)
Nucleoside monophosphate binding induces further changes that render the enzyme catalytically capable of facilitating a transfer of the phosphoryl group from ATP to nucleoside monophosphate. (wikipedia.org)
This enzyme mechanism is an example of catalysis by approximation: the nucleoside-phosphate kinase binds the substrates to bring them together in the correct position for the phosphoryl group to be transferred. (wikipedia.org)
They are involved in pharmaceutically important enzyme reactions including kinases and phosphodiesterases. (edu.au)
OMP is then decarboxylated by the enzyme OMP decarboxylase to yield uridine monophosphate (UMP). (biologyonline.com)
Highly selective for virus-infected cells because of its high affinity for viral thymidine kinase enzyme. (medscape.com)
The role of the C8-H of ATP in the binding and/or phosphoryl transfer on the enzyme activity of a number of kinase and synthetase enzymes has been elucidated. (biomedcentral.com)
The intrinsic catalysis rate mediated by each kinase enzyme is complex, yielding apparent K M values ranging from less than 0.4 μM to more than 1 mM for ATP in the various kinases. (biomedcentral.com)
We have demonstrated the mechanism by which the enzyme activities of Group 2 kinases, shikimate kinase (SK) and adenylate kinase 1 (AK1), are controlled by the C8-H of ATP. (biomedcentral.com)
The lability of the C8-H mediated by active site residues co-ordinated to the purine ring of ATP therefore plays a significant role in explaining the broad K M range associated with kinase steady state enzyme activities. (biomedcentral.com)
The 5'-nucleotidase (NT5) family of enzyme dephosphorylates non-cyclic nucleoside monophosphates to produce nucleosides and inorganic phosphates. (diva-portal.org)
A new subfamily of short bacterial adenylate kinases with the Mycobacterium tuberculosis enzyme as a model: a predictive and experimental study. (rhea-db.org)
A sequence comparison with 52 different forms of adenylate kinases (AK) suggests that the enzyme from M. tuberculosis belongs to a new subfamily of 'short' bacterial AKs. (rhea-db.org)
Schultz CP, Ylisastigui-Pons L, Serina L, Sakamoto H, Mantsch HH, Neuhard J, Barzu O, Gilles AM: Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli. (drugbank.com)
Although the rate of incorporation of triphosphate 5 into RNA by E 64d small molecule kinase inhibitor PV RdRP was slower than RTP and natural nucleoside triphosphates, both 5 and diphosphate 6 were much more potent inhibitors of this enzyme. (irjs.info)

When the diet contains nucleotides, the body digests them by nucleotidases to produce nucleosides and phosphates. (biologyonline.com)

These are nucleotides consisting of a purine base linked to a ribose to which one monophosphate group is attached. (ymdb.ca)
The article was also received and subsequently accepted and published by Nucleosides, Nucleotides this website and Nucleic Acids [3]. (peptide-solubility.com)
Adenosine kinase in red blood cells converts adenosine into adenine nucleotides. (turkupetcentre.net)
PV RdRP incorporated 5 into RNA very slowly relative to the correct nucleotides[36] and with a low apparent = 50 M) whereas monophosphate 7 and nucleoside 3 lacked any detectable inhibitory activity. (irjs.info)

3 This offers a potentially powerful tool for high-throughput screening of kinase inhibitors, obviating the need for expensive antibodies or labelled substrates. (edu.au)
PSI-353661 is active against replicons harboring the NS5B S282T or S96T/N142T amino acid alterations that confer decreased susceptibility to nucleoside/tide analogs as well as mutations that confer resistance to non-nucleoside inhibitors of NS5B. (inrae.fr)

This process involves a specific transmembrane nucleoside carrier system that is reversible, nonconcentrative, and bidirectionally symmetrical. (nih.gov)
The intracellular uptake of adenosine is mediated by a specific transmembrane nucleoside transport system. (globalrph.com)

Nucleoside analogs are phosphorylated initially by viral thymidine kinase to eventually form a nucleoside triphosphate. (medscape.com)
FUNCTION: ATP dependent phosphorylation of adenosine and other CC related nucleoside analogs to monophosphate derivatives. (linuxfocus.org)

FUNCTION: Catalyzes the phosphorylation of pyrimidine nucleoside CC monophosphates at the expense of ATP. (expasy.org)
Definition noun plural: cytidines cy·ti·dine, ˈsaɪtɪdiːn A pyrimidine nucleoside that has cytosine attached to the pentose sugar ribose Details Overview A nucleoside is a nucleobase with a five-carbon sugar (either ribose or deoxyribose). (biologyonline.com)

This effect serves to concentrate acyclovir monophosphate into virus-infected cells. (medscape.com)
To become active and inhibit viral DNA synthesis acyclovir must be phosphorylated to acyclovir monophosphate by specific HSV-1 thymidine kinases (tk) and then phosphorylated to acyclovir triphosphate, the active form of acyclovir able to inhibit viral DNA-polymerases, by host cell kinases. (biomedcentral.com)

Adenosine monophosphate formed by phosphorylation of adenosine is incorporated into the high-energy phosphate pool. (nih.gov)

Eventually, uridine diphosphate (UDP) and uridine triphosphate (UTP) are produced down the biosynthetic pathway by kinases and dephosphorylation of ATPs. (biologyonline.com)

Similar catalytic domains are present in a variety of proteins, including: ATP synthase Myosin, and other molecular motor proteins G protein and other proteins involved in signal transduction Helicases for unwinding DNA and RNA Pyrimidine metabolism When a phylogenetic tree composed of members of the nucleoside-phosphate kinase family was made, it showed that these enzymes had originally diverged from a common ancestor into long and short varieties. (wikipedia.org)
We hypothesized that gene silencing of NT5 enzymes to increase the intracellular availability of AMP would increase AMP-activated protein kinase (AMPK) activity and metabolism. (diva-portal.org)
We then examined the pharmacological effects of the inhibition of the N-methyl-D-aspartate (NMDA) glutamate receptor and protein kinase Cγ (PKCγ) on alcohol drinking in wild-type mice. (elsevierpure.com)
Results: We identified that NMDA glutamate receptor-mediated downregulation of intracellular PKCγ-neurogranin- calcium-calmodulin dependent protein kinase type II signaling is correlated with reduced cyclic adenosine monophosphate response element binding activity in ENT1 -/- mice. (elsevierpure.com)
By targeting electron transport chain complex 1 and independently of AMP-activated protein kinase. (lu.se)
By targeting electron transport chain complex 1 and independently of AMP-activated protein kinase (AMPK) or NF-κB, metformin blocked LPS-induced and ATP-dependent mitochondrial (mt) DNA synthesis and generation of oxidized mtDNA, an NLRP3 ligand. (lu.se)

Synthetic purine nucleoside analogue with activity against a number of herpesviruses, including herpes simplex and varicella-zoster. (medscape.com)
Adenosine is an endogenous purine nucleoside, composed of nucleobase adenine and β-D-ribose moieties. (turkupetcentre.net)
Adenosine is rapidly catabolized by adenosine deaminases to inosine, which can be further deribosylated by purine nucleoside phosphorylase to hypoxantine . (turkupetcentre.net)
Diagnosis of purine nucleoside phosphorylase deficiency is by DNA analysis. (msdmanuals.com)
Treatment of purine nucleoside phosphorylase deficiency is with bone marrow or stem cell transplantation. (msdmanuals.com)

T4 Polynucleotide Kinase can catalyze the transfer of the γ-phosphate group of ATP to the 5´-hydroxyl end and 3´-monophosphate nucleoside of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA). (cellagentech.com)
T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of Pi from the gamma position of ATP to the 5´ -hydroxyl terminus of (ds and ss) DNA, RNA and nucleoside 3´-monophosphates. (alpha-zyme.com)

IMP is then converted into either adenosine monophosphate (AMP) or guanosine monophosphate (GMP). (biologyonline.com)

Background: Mice lacking type 1 equilibrative nucleoside transporter (ENT1 -/- ) exhibit increased ethanol-preferring behavior compared with wild-type littermates. (elsevierpure.com)
Dipyridamole elevates extracellular adenosine concentrations by inhibiting equilibrative nucleoside transporters (ENTs). (turkupetcentre.net)

In addition, we investigated in vivo cyclic adenosine monophosphate response element binding activity using cyclic adenosine monophosphate response element-β-galactosidase mice in an ENT1 -/- background. (elsevierpure.com)
It has a central role in cell metabolism and signalling as such and as the backbone for ATP (adenosine triphosphate) and cAMP (cyclic adenosine monophosphate). (turkupetcentre.net)

As for the nitrogenous base (or nucleobase) component, the common bases are adenine (A), guanine (G), cytosine (C), and uracil (U). Depending on the number of phosphates that make up the compound, the ribonucleotide may be designated as monophosphate (having only one phosphate group), diphosphate (having two phosphate groups), and triphosphate (having three phosphate groups). (biologyonline.com)

Using a combination of ATP deuterated at the C8 position (C8D-ATP) as a molecular probe with site directed mutagenesis (SDM) of conserved amino acid residues in shikimate kinase and adenylate kinase active sites, we have elucidated a mechanism by which the ATP C8-H is induced to be labile in the broader kinase family. (biomedcentral.com)

Catalysis of the reaction: ATP + nucleoside monophosphate = ADP + nucleoside diphosphate. (virtualflybrain.org)

We have developed a Eu(III) receptor that selectively binds to ADP, providing a long-lived luminescence signal that enables real-time monitoring of kinase activity. (edu.au)
Also displays broad nucleoside diphosphate CC kinase activity. (expasy.org)
A phosphoramidate prodrug of 2'-deoxy-2'-alpha-fluoro-beta-C-methyluridine-5'-monophosphate, PSI-7851, demonstrates potent anti-hepatitis C virus (HCV) activity both in vitro and in vivo. (inrae.fr)
MZB is a nucleoside of the imidazole class, and was found to have weak antimicrobial activity against Candida albicans , but it proved ineffective against experimental candidiasis [ 1 ]. (hindawi.com)

AICA-Riboside, CAS 2627-69-2, is a cell-permeable nucleoside compound whose phosphorylated metabolite activates AMPK and acts as a regulator of de novo purine synthesis. (emdmillipore.com)

Once inside the cell, adenosine is rapidly phosphorylated by adenosine kinase to adenosine monophosphate, or deaminated by adenosine deaminase to inosine. (globalrph.com)

The monophosphate then is metabolized into the triphosphate active form by cellular kinases. (medscape.com)
RDV is taken up in the target cells and metabolized in multiple steps to form the active nucleoside triphosphate (TP) (GS-443902), which in turn acts as a potent and selective inhibitor of multiple viral RNA polymerases. (inrae.fr)
The monophosphate is then consecutively phosphorylated to diphosphate and triphosphate by cellular phosphotransferases. (inrae.fr)

RT "Cloning and expression of the adenosine kinase gene from rat and RT human tissues. (linuxfocus.org)

A nucleoside is a nucleobase with a five-carbon sugar (either ribose or deoxyribose). (biologyonline.com)

The s/s-U template was used E 64d small molecule kinase inhibitor for primer extension by the PV RdRP (3Dpol) in the presence of Mn2+. (irjs.info)
Significantly, both 3 and 1 considerably decreased the E 64d small molecule kinase inhibitor titer of poliovirus. (irjs.info)

This study suggests P. sidoides is potentially a source of anti-tubercular compounds and the Mtb kinase platform has significant potential as a tool for the subsequent screening of P. sidoides extracts and plant extracts in general, for compound identification and elaboration by selected extract target inhibitor profiling. (biomedcentral.com)
Adenosine monophosphate is a strong basic compound (based on its pKa). (ymdb.ca)

Although less conserved, the earliest sporulation genes of sensory histidine kinases could not be positively assigned among 59 histidine kinase genes in the genome (Figure 8). (peptide-solubility.com)

SIMILARITY: Belongs to the adenylate kinase family. (expasy.org)
TMPK from Mycobacterium tuberculosis belongs to the NMPK family, which was chosen as potential targets for the development of new antimicrobials (see "Nucleoside monophosphate kinases" project). (pasteur.fr)
Adenosine monophosphate, also known as adenylic acid or 5'-AMP, belongs to the class of organic compounds known as purine ribonucleoside monophosphates. (ymdb.ca)
belongs to the class of organic compounds known as purine ribonucleoside monophosphates. (ymdb.ca)

After the pyrimidine ring forms, 5-phospho-α-D-ribosyl 1-pyrophosphate (PRPP), a ribose phosphate, reacts to orotate to form orotidine-5-monophosphate (OMP). (biologyonline.com)

Following the evolution of long and short varieties of NMP-kinases, smaller changes in the amino acid sequences resulted in the differentiation of subcellular localization. (wikipedia.org)

Adenosine is antagonized competitively by methylxanthines such as caffeine and theophylline, and potentiated by blockers of nucleoside transport such as dipyridamole. (nih.gov)

Intracellular adenosine is rapidly metabolized either via phosphorylation to adenosine monophosphate by adenosine kinase, or via deamination to inosine by adenosine deaminase in the cytosol. (nih.gov)
Since adenosine kinase has a lower K m and V max than adenosine deaminase, deamination plays a significant role only when cytosolic adenosine saturates the phosphorylation pathway. (nih.gov)

The aim of this study was to develop a platform of Mycobacterium tuberculosis (Mtb) kinase enzymes that may be used for the identification of therapeutically relevant ethnobotanical extracts that will allow drug target identification, as well as the subsequent isolation of the active compounds. (biomedcentral.com)
It has been demonstrated that the adenyl moiety of ATP plays a direct role in the regulation of ATP binding and/or phosphoryl transfer within a range of kinase and synthetase enzymes. (biomedcentral.com)

Our data support the hypothesis that the unique properties of RDV prodrug not only allow lung-specific accumulation critical for the treatment of respiratory viral infection such as COVID-19, they also enable efficient intracellular metabolism of RDV and its Met X to monophosphate and successive phosphorylation to form the active TP in disease-relevant cells. (inrae.fr)

60 known mutations) results in accumulation of adenosine , which is converted to its ribonucleotide and deoxyribonucleotide (dATP) forms by cellular kinases. (msdmanuals.com)

UMP-CMP kinase CC subfamily. (expasy.org)

We are developing luminescent europium(III) complexes 2-6 capable of reversibly binding and discriminating between nucleoside tri-, di- and monophosphate anions in aqueous biological media (Figure 1). (edu.au)
The 50% inhibitory concentration (IC 50 ) of an aqueous root extract of P. sidoides against the kinases indicated SK has an IC 50 of 1.2 μg/ml and GK 1.4 μg/ml. (biomedcentral.com)

Chemicals and Drugs7

Phenomena and Processes2

Information Science2

Cytidine10

Nucleotide8

Diphosphate kinase1

Enzyme17

Produce nucleosides1

Nucleotides4

Inhibitors2

Specific transmembrane nucleoside2

Analogs2

Pyrimidine nucleoside2

Acyclovir monophosphate2

Phosphorylation of adenosine1

Uridine1

Protein6

Purine nucleoside5

Polynucleotide Kinase2

Guanosine monophosphate1

Equilibrative nucleoside2

Cyclic2

Phosphates1

Shikimate1

Catalysis1

Activity4

Synthesis1

Deaminated by adenosine1

Triphosphate3

Gene1

Nucleobase1

Small molecule2

Compound2

Histidine1

Belongs4

Ribose1

Amino acid1

Dipyridamole1

Adenosine deaminase2

Enzymes2

Intracellular1

DATP1

Subfamily1

Aqueous2