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  • oocytes
  • in their absence, the oocytes complete maturation and can be fertilized, but no nucleoli are formed in the zygote or embryo, leading to developmental failure. (biologists.org)
  • Fully grown mammalian oocytes contain specific nucleoli, termed the nucleolus precursor bodies (NPBs), which exhibit distinct structural and functional differences compared with nucleoli in somatic cells ( supplementary material Fig. S1 ). (biologists.org)
  • Nucleoplasm
  • The authors used fluorescence recovery after photobleaching (FRAP) and inverse FRAP (iFRAP) to show that nucleostemin tagged with green fluorescent protein shuttled rapidly and bidirectionally between the nucleolus and the nucleoplasm of cultured CHO and U2 OS cells. (sciencemag.org)
  • protein
  • The nucleolus ultrastructure can be visualized through an electron microscope , while the organization and dynamics can be studied through fluorescent protein tagging and fluorescent recovery after photobleaching ( FRAP ). (princeton.edu)
  • Prof. Antebi from the Max Planck Institute for Biology of Ageing in Cologne notes that "the nucleolus contains a protein highly conserved in evolution called fibrillarin, which also decreases during infection. (mpg.de)
  • They observed that long-lived mutants with small nucleoli also show reduced levels of a nucleolar protein called fibrillarin, which aids in the assembly of the protein factory. (innovations-report.com)
  • Researchers have known for some time that protein folding helpers, so-called chaperones, move into the nucleolus under certain circumstances. (innovations-report.com)
  • We were able to show that stressing cells by heating them to 43°C, results in the transport of the misfolded luciferase protein together with the chaperones into the nucleolus. (innovations-report.com)
  • Together they were able to show that the misfolded luciferase protein behaved differently within the nucleolus than in the rest of the cell. (innovations-report.com)
  • Nucleostemin, a protein found in the nucleoli of highly proliferative cells (such as stem cells and some cancer cell lines), may help regulate cell proliferation. (sciencemag.org)
  • The more protein that a cell needs, the larger the nucleolus is likely to be. (reference.com)
  • Researchers have determined that the protein nucleophosmin (NPM1) serves as glue that holds proteins and RNA together in the nucleolus and showed how NPM1's structure makes it ideal for the job. (phys.org)
  • NPM1 is an important regulatory protein that is abundant in the nucleolus. (phys.org)
  • That suggests that NPM1 is probably not the only protein contributing to phase separation in the nucleolus, but our studies show that it certainly is a very important player. (phys.org)
  • NORs
  • In principle, the labelling patterns correspond to the patterns of the silver-stained nucleolus organizing regions (NORs) in the same species. (springer.com)
  • Schubert I (1984) Mobile nucleolus organizing regions (NORs) in Allium (Liliaceae s. lat. (springer.com)
  • molecules
  • The nucleolus is home to a vast array of proteins, RNA and other molecules, but until this study its molecular foundation was unknown. (phys.org)
  • NPM1 forms networks of interactions with other molecules in the nucleolus, loosely gluing nucleolar components together. (phys.org)
  • The nucleolus performs a specialized function, and NPM1 seems to have evolved to assist in the process by being able to phase separate with these two important and very different types of nucleolar molecules," said corresponding author Richard Kriwacki, Ph.D., a member of the St. Jude Department of Structural Biology. (phys.org)
  • NM95
  • MAb NM95 recognizes an antigen associated with the nucleoli in human cells. (novusbio.com)
  • Catalog number key for antibody number 0095, Anti-Nucleoli (NM95) The prefix indicates conjugation, followed by the antibody number and size suffix. (biotium.com)
  • fragmentation
  • Such disruption of nucleolar activity can lead to misfolded RNAs, misassembled ribonucleoprotein complexes, and fragmentation of the nucleolus. (frontiersin.org)
  • Here is presented new details to the hypothesis, explaining how the disrupted chromatin can lead to subsequent disruption of the nucleolus, even nucleolar fragmentation, which results in ineffective nucleolar functioning, misfolded RNAs, misassembled or incompletely assembled ribonucleoprotein (RNP) complexes, and stabilization of nucleolar components in autoantigenic conformations. (frontiersin.org)
  • vitro
  • High power TEM of part of a pronuclear nucleolus in a stage 1b embryo in vitro , 17 hours post-insemination (original magnification x51,710). (ehd.org)
  • Mitotic
  • The prolymphocytoid variant of MCL was characterized by morphologic features similar to the paraimmunoblastic variant of small lymphocytic lymphoma with sheets of intermediate-sized to large cells with a single prominent nucleolus associated with a brisk mitotic rate. (thefreedictionary.com)
  • found
  • The nucleolus (also called nucleole ) is a non-membrane bound structure [ 1 ] composed of proteins and nucleic acids found within the nucleus . (princeton.edu)
  • When we analyzed muscle biopsies from individuals older than sixty years that underwent modest dietary restriction coupled with exercise - a common way to prolong lifespan and increase health - we found that they had smaller nucleoli in their muscle cells after the intervention than before. (innovations-report.com)
  • They found that the SR peptides targeted the nucleolus. (alzforum.org)
  • embryo
  • It is commonly accepted that, as the mammalian embryo develops, NPBs are gradually transformed into nucleoli that are typical for differentiated cells ( Fléchon and Kopecny, 1998 ). (biologists.org)
  • pathway
  • All of the mutants showed smaller nucleoli than their shorter-lived relatives, independent of the pathway that led to their longevity. (innovations-report.com)
  • Here, we will also discuss evidence for crosstalk between the NF-κB pathway and nucleoli, the role that this cross-talk has in the anti-tumour effects of NSAIDs and ways forward to exploit this crosstalk for therapeutic purpose. (mdpi.com)