Isolating nucleic acidsGeneticsAcids Res2021EnzymesGenomicsResearchBiotechnologyProteinMechanismsAmplificationProteinsSequenceGeneticSequencesEvolutionAcid extractionDeoxyribonucleicRecombinationLaboratory of MoleBackbonePredictionProfessorPhosphateVariety of molecularDetectionEvolutionary biologyBiologistsCore FacilitySearchModuleAnalysisBiological2022NucleotidesPrimersComputationalPrinciplesContentStructural biology
Isolating nucleic acids1
- Despite phenol′s incomplete inhibition of RNase activity, it possesses characteristics that make it useful for isolating nucleic acids when combined with chloroform and alcohol. (sigmaaldrich.com)
Genetics2
- Twentieth-century developments in biology and statistics established genetics as a science and led to the discovery of causal loci for many single-gene disorders. (cdc.gov)
- The essential biochemistry, genetics, cell biology, and metabolic pathways underlying the survival and success of all living organisms. (queensu.ca)
Acids Res9
- Nucleic Acids Res. (crossref.org)
- However, chemical and enzymatic probing has lead to the idea that these tRNAs have retained the basic cloverleaf structure of canonical tRNAs but that they lack several conserved tertiary interactions leading to a weaker three-dimensional structure (4Watanabe Y.-I. Kawai G. Yokogawa T. Hayashi N. Kumazawa Y. Ueda T. Nishikawa K. Hirao I. Miura K.-I. Watanabe K. Nucleic Acids Res. (ncsu.edu)
- 22: 5378-5384Crossref PubMed Scopus (56) Google Scholar, 5Yokogawa T. Watanabe Y.-I. Kumazawa Y. Ueda T. Hirao I. Miura K.-I. Watanabe K. Nucleic Acids Res. (ncsu.edu)
- 19: 6101-6105Crossref PubMed Scopus (68) Google Scholar, 6Wakita K. Watanabe W. Yokogawa T. Kumazawa Y. Nakamura S. Ueda T. Watanabe K. Nishikawa K. Nucleic Acids Res. (ncsu.edu)
- The diseases associated with mitochondrial tRNA mutations may arise from failure in the processing of the tRNA (13Levinger L. Jacobs O. James M. Nucleic Acids Res. (ncsu.edu)
- As a result of this dual role, mitochondrial Met-tRNAMet must be recognized by the mitochondrial Met-tRNA transformylase (MTFmt) and be brought as fMet-tRNAMet to the ribosome for translational initiation (19Spencer A.C. Spremulli L.L. Nucleic Acids Res. (ncsu.edu)
- E. coli BL21 cells, carrying the pET15-bovine MTFmt plasmid construct, were grown as described (19Spencer A.C. Spremulli L.L. Nucleic Acids Res. (ncsu.edu)
- 33: 241-252Crossref PubMed Scopus (30) Google Scholar, 35Yarian C.S. Basti M.M. Cain R.J. Ansari G. Guenther R.H. Sochacka E. Czerwinska G. Malkiewicz A. Agris P.F. Nucleic Acids Res. (ncsu.edu)
- However, small amounts of the aminoacylated 8U→C mutated hmtRNAMet could be isolated, permitting a limited investigation of additional steps in translation.In the mammalian mitochondrial system, the Met-tRNAMet must be formylated by the mitochondrial transformylase (MTFmt) to be used in initiation (19Spencer A.C. Spremulli L.L. Nucleic Acids Res. (ncsu.edu)
Enzymes2
- They would then label it using, for example, radioisotopes or enzymes, and subject it to hybridization with the nucleic acids of the pathogen to be tested. (iso.org)
- You are unlikely to find references to a single compound, but may be able to find useful books on classes of compounds, such as carbohydrates, nucleic acids, proteins or enzymes. (ucsb.edu)
Genomics2
- The Molecular Biology and Functional Genomics Core Facility offer a range of services combining advice and access to cutting-edge techniques to meet gene analysis needs. (ircm.qc.ca)
- COVID-19 has become the most widespread global pandemic in the past 100 years, and it is also the first time that molecular biology genomics has been applied on a super-large scale in response to an emerging outbreak. (iso.org)
Research13
- Source code developed for this project is available at https://github.com/bio-ontology-research-group/phenomenet-vp , and analysis results at http://www.cbrc.kaust.edu.sa/onto/pvp/ . (plos.org)
- To connect practical aspects of structural and chemical biology to theory, and thereby creating coherence to further the student's understanding and aptitude to research at the interface of traditional academic disciplines. (le.ac.uk)
- I have been teaching a variety of courses here at Queen's for over 20 years, from introductory biology to the commercialization of biological research at the graduate level. (queensu.ca)
- As a scientist, I have 30 years of experience conducting research on various molecular problems using a range of organisms, from bacteria to plants. (queensu.ca)
- Nucleic Acids Research, 38(9), 3094–3105. (ncsu.edu)
- NUCLEIC ACIDS RESEARCH, 36(20), 6548–6557. (ncsu.edu)
- The discovery of DNA in 1953 enabled a new era in molecular biology, moving pathogen research forward from the morphological level to the molecular level. (iso.org)
- M. Madan Babu is the Endowed Chair of Biological Data Science in the Department of Structural Biology at St Jude Children's Research Hospital. (epfl.ch)
- Madan is the Chief Editor of Molecular Systems Biology and an Executive Editor of Nucleic Acids Research. (epfl.ch)
- This provides the opportunity for ecologists, evolutionary biologists and molecular biologists to incorporate bioinformatic analyses into their existing research program to approach their research questions from an interdisciplinary angle. (lu.se)
- Several downstream analyses are performed and their utility in applied ecology, evolutionary biology and molecular biology research will be discussed with guest speakers. (lu.se)
- Nucleic Acids Research 2015, June 19. (lu.se)
- Nucleic Acids Research 2014, Jul 22. (lu.se)
Biotechnology2
- Most major advances in microbiology have occurred within the past 150 years, and several important subdisciplines of microbiology have developed during this time, including microbial ecology, molecular biology, immunology, industrial microbiology, and biotechnology. (researchgate.net)
- Madan did his post-doc from the NCBI, NIH (2006), received his Ph.D. from the MRC Lab of Molecular Biology and Trinity College, Cambridge University, UK (2004) and his undergraduate degree from the Centre for Biotechnology at Anna University in India (2001). (epfl.ch)
Protein7
- Since 2001 he had been working at the Laboratory of protein and nucleic acids of IMBB as: laboratory assistant - junior researcher - researcher - senior researcher. (org.kz)
- Between 2016 and 2022 - Leading researcher at the Laboratory of protein and nucleic acids of IMBB. (org.kz)
- I'm happy to have identified a new one in ProNIT, whose thermodynamic data for protein-DNA interactions is "very useful for understanding the principles of molecular recognition. (genengnews.com)
- Computational studies and Bioinformatics is an interdisciplinary field that progresses and applies computational methods to explore biological data collections such as protein samples, cell populations and genetic sequences for newer predictions or find out new Biology. (wikicfp.com)
- It plays an invaluable role in protein biochemistry, and is making an increasing contribution to modern molecular biology and to the interpretation of DNA sequences. (the-scientist.com)
- The advent in recent years of rapid nucleic acid sequencing techniques has taken much of the drudgery away from direct amino acid sequencing of large proteins, and has instead put more emphasis on the importance of determining at least limited amounts of sequence directly from the protein of interest. (the-scientist.com)
- The RNA encoded information is than translated into protein , via bio-synthetic mechanism in the cytoplasm of the cell. (herbs2000.com)
Mechanisms3
- My laboratory works on the genetic and molecular mechanisms that regulate aging and aging-related disease. (berkeley.edu)
- Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)
- The need to understand the molecular mechanisms in species has made the PCR an indispensable tool for understanding the functioning of these biological systems. (intechopen.com)
Amplification6
- LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. (neb.com)
- Recently published, ISO/TS 5798 provides recommendations for the design, development, verification, validation and implementation of analytical tests for detecting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) using nucleic acid amplification. (iso.org)
- ISO/TS 5798 mainly focuses on the use of nucleic acid amplification methods for diagnosis and screening of SARS-CoV-2 and proposes requirements and recommendations for the design, development, validation, verification and implementation of testing methods. (iso.org)
- But it also covers the steps of the overall process of pre-examination, examination and post-examination for the analysis of nucleic acid amplification method for the detection of SARS-CoV-2, and specifies the methods and comprehensive assessment indicators including accuracy, limit of detection, inclusivity, and specificity, etc. (iso.org)
- RESUME Une PCR (amplification en chaîne par polymérase) diagnostique avec des amorces spécifiques d'espèces et un séquençage direct ont été utilisés pour la détermination des membres du complexe Ano- pheles maculipennis dans les régions du nord-ouest et du centre de la République islamique d'Iran. (who.int)
- After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
Proteins3
- We are interested in understanding at the molecular level how recombination occurs and what roles are played by the many proteins involved in DNA recombination, repair and replication. (brandeis.edu)
- Antibodies recognize specific configurations (epitopes, or antigenic determinants) on the surfaces of antigens (eg, proteins, polysaccharides, nucleic acids). (msdmanuals.com)
- Applications are invited for two postdoctoral positions to develop computational approaches to study (1) the phenomena of liquid-liquid phase separation of heterogenous mixtures of nucleic acids and proteins occurring inside the Cell nucleus and (2) the organization of chromatin with subnucleosome resolution inside megabase scale domains. (lu.se)
Sequence6
- A nucleic acid sequence is the order of nucleotides within a DNA (GACT) or RNA (GACU) molecule that is determined by a series of letters. (wikipedia.org)
- Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its sequence. (wikipedia.org)
- many molecules have several possible three-dimensional structures, so predicting these structures remains out of reach unless obvious sequence and functional similarity to a known class of nucleic acid molecules, such as transfer RNA (tRNA) or microRNA (miRNA), is observed. (wikipedia.org)
- A common problem for researchers working with RNA is to determine the three-dimensional structure of the molecule given only a nucleic acid sequence. (wikipedia.org)
- Using synchronized cells undergoing recombination that is initiated at a specific site on a chromosome by an inducible endonuclease, we use physical monitoring techniques (Southern blots, PCR analysis) to follow the sequence of molecular events that occur in real time. (brandeis.edu)
- Basically, technicians synthesize a single-stranded nucleic acid sequence complementary to the DNA or RNA of a specific pathogen as a probe. (iso.org)
Genetic4
- Methods of molecular biology and genetic engineering to create structures that allow to express recombinant genes. (org.kz)
- All genetic information needed for life and the inheritance of characters is encoded in biomolecules known as nucleic acids. (herbs2000.com)
- In this way the monomers are interlinked to form a nucleic acid polymer holding all the genetic information necessary for life. (herbs2000.com)
- Genetic information is stored along the nucleic acid chain because all the bases in the nucleotides form hydrogen bonds with each other in a specific way - this ensures what is called base pairing. (herbs2000.com)
Sequences1
- Secondary structure can be predicted from one or several nucleic acid sequences. (wikipedia.org)
Acid extraction2
Deoxyribonucleic1
- There are two forms of nucleic acid - DNA or deoxyribonucleic acid and RNA or ribonucleic acid, both of these forms are the basis of all life and occur in all living cells. (herbs2000.com)
Recombination1
Laboratory of Mole1
- Before joining St Jude, Madan was a Programme Leader at the MRC Laboratory of Molecular Biology in Cambridge, UK (2006-2020). (epfl.ch)
Backbone1
- This is partly because the extra oxygen in RNA increases the propensity for hydrogen bonding in the nucleic acid backbone. (wikipedia.org)
Prediction1
- This article is about the computational prediction of nucleic acid structure. (wikipedia.org)
Professor2
- Ms. Shweta Singh Assistant Professor Biyani Groups of colleges, Jaipur explains about basic introduction to nucleic acids which are important for cellular life and survival. (gurukpo.com)
- I am a Professor in the Department of Biology at Queen's University. (queensu.ca)
Phosphate3
- There are three potential metal binding groups on nucleic acids: phosphate, sugar, and base moieties. (wikipedia.org)
- One flat, heterocyclic, nitrogen rich organic base and lastly one phosphate group - this last unit of the nucleic acid polymer is responsible for the acidic nature of the nucleic acid as it is very negative in charge. (herbs2000.com)
- The addition of the phosphate group also covalently connected to the sugar unit completes the basic component of the nucleic acid polymer. (herbs2000.com)
Variety of molecular2
- Phenol solution can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols. (sigmaaldrich.com)
- Beyond the variety of molecular implementations of such patterns, we emphasize the unitary principles associated with them, across scales in space and time, within a general mechanochemical framework. (mpi-cbg.de)
Detection2
- Nucleic acid testing, which operates on this molecular level, has gradually become the mainstream of pathogen detection. (iso.org)
- Whole-genome SNP analysis applied directly to stool for genotyping Shiga toxin-producing Escherichia coli : An advanced molecular detection method for foodborne surveillance and outbreak tracking. (cdc.gov)
Evolutionary biology1
Biologists1
- Finding useful thermodynamic information of interest to molecular biologists is not particularly easy. (genengnews.com)
Core Facility1
- 5′-32P-GAACCGGACCGAAGCCCG, obtained from the Nucleic Acids Core Facility at University of North Carolina) was added to the 1M7-treated, DMSO-treated, or untreated RNA (2 pmol), and the samples were incubated at 65 °C for 5 min and then at 35 °C for 20 min for primer annealing. (ncsu.edu)
Search2
- To locate books on your topic, use UC Library Search ( https://search.library.ucsb.edu/discovery/search?vid=01UCSB_INST%3AUCSB&lang=en . (ucsb.edu)
- For more general searches, try a subject search on 'biochemistry' or 'molecular biology', then browse through the suggested alternative subject headings to see if a more specific one is recommended before browsing the titles under 'Biochemistry' itself. (ucsb.edu)
Module2
- Welcome to the Nucleic Acid Techniques Module. (hawaii.edu)
- In this module you will learn about techniques that researchers use to study the nucleic acids, DNA and RNA. (hawaii.edu)
Analysis3
- Topics covered include extraction method selection, basic extraction steps, and nucleic acid analysis. (cdc.gov)
- We used standard molecular biology techniques for nucleic acid preparation and analysis. (cdc.gov)
- The study of biological complexity is a new frontier that requires high-throughput molecular technology, high speed computer memory, new approaches to data analysis, and the integration of interdisciplinary skills. (intechopen.com)
Biological4
- Major technological advances in affordable nucleic acid sequencing have allowed for an explosion of sequencing data and molecular tools available for researchers in biological sciences. (lu.se)
- The successful candidate will have strong experience in molecular modelling and simulations and statistical mechanics and will hold a PhD/DPhil in physics, chemistry, quantitative biology, applied mathematics, or a closely related field, with a strong interest in biological systems. (lu.se)
- Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)
- 1974. In: Biological handbooks: Biology data book, Volume III, second edition. (cdc.gov)
20221
Nucleotides2
- Nucleic acids are formed when nucleotides come together through phosphodiester linkages between the 5' and 3' carbon atoms. (wikipedia.org)
- The basic components of the nucleic acids, the nucleotides are structurally formed from three major units. (herbs2000.com)
Primers1
Computational1
Principles1
- This basic Molecular Biology course series will introduce the scientific background for molecular diagnosis, the principles of molecular biology laboratory practice, and common methods. (cdc.gov)
Content1
- Content Tutorials: important background information, detailed text and illustrations that describe and depict the molecular biology significance, practical uses, and procedures. (hawaii.edu)